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Gene information
General information
TC012586 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11729444..11732028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012586
      iBeetle screen iB_10977

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax segment sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012586 RA
        MSRVPRLKT ETSQRVVPD PASSRLPRG VSRVTEKSQ 
        RNPRSGVGR QLGVVKPEG TPQPGPSRF QQGVFKQTL 
        AKSVADPGK KVRTVSASH SQIKPPKVQ TKRGEECVV 
        QQMSLETTV QRCPRAEYI GKTMRGVDV APSGVARQL 
        ELTSQQIDP TRGTESAGV NETVSVTST LSITSGGKQ 
        LKKTYKKQT AIASKLAKK PNLNPHHIN RLVKEETED 
        VENTLKMVP PSLVSSSID KFPLKKKLQ KLRLNAEPV 
        SLLTQSVIH PAPTADEVD AEAPPELMA TVMDAVEKG 
        ATPDSAPNQ IFNSLFPEV HEARIRVSS VAPGPLEDV 
        VVPTDPAVR VVDEALSSE MEEFIASEI QNKQELPTD 
        DLCSENNIS ATERAAKGE NKAPEMFLE GEPAPWEDF 
        TVRKISTLV GGDPIDLLD TSDLSALET SADISKELA 
        NGIDYFLDA DVVPYDTKI QIRGSKIPA WFPGYVSAF 
        PHLPELVKV TKSPSEVLH AAWKAVQPK VFPDEPLLI 
        V
      CDS sequence
      • >TC012586 RA
        atgtcccgt gttcctcga cttaaaacg gaaacctcc 
        cagcgtgtg gtcccagac cccgcttca tcccgactc 
        cctcgcgga gtttcccgt gtgactgaa aagtcccag 
        cgcaatccc cggtcggga gtcgggcgt cagctcgga 
        gttgtgaaa cctgaaggg actccgcaa ccagggccc 
        tcgagattc caacaaggc gtcttcaag caaaccttg 
        gccaagtca gtggcggac cctgggaag aaggtgcgg 
        acggtgtct gcctcacac tcacagatt aaacccccc 
        aaagtgcaa acgaagcga ggggaggaa tgtgtggtg 
        cagcagatg tcgctggag acgacggtg cagaggtgc 
        cccagggcg gagtacatc ggcaagacc atgcgaggc 
        gtcgacgtg gccccctcg ggggtggcg cgccagctg 
        gaactcacc tcgcaacag atcgacccg acacgtgga 
        acggaatcg gccggtgtc aacgaaact gtttcggtc 
        actagcacg ctctcgatt acttcggga gggaagcag 
        ttgaagaaa acttacaag aagcagacc gctattgcc 
        tcgaaattg gccaaaaag ccgaatttg aacccgcac 
        cacatcaat cggctggtt aaggaggag accgaagac 
        gtggaaaac accttgaag atggtgcct ccgagtcta 
        gtctcctcg tccatcgac aagtttcct ttgaagaaa 
        aagttgcaa aagttgcgg cttaacgcg gagcctgtg 
        agtctttta actcagtcg gtcatccat cctgcgccc 
        actgctgac gaggtcgat gccgaagcg cccccggag 
        ctgatggcc acggtcatg gatgccgtc gaaaagggg 
        gcgacgccg gactcggcc cctaaccag attttcaac 
        agtctcttc cccgaagtg cacgaggcg cggattagg 
        gtcagttcg gtggctccc gggcctttg gaggatgtt 
        gtcgtcccc acggatccc gccgtgagg gtggtcgac 
        gaggctctg tcttccgaa atggaggag tttattgct 
        tccgaaatc cagaacaag caagagttg cccacggat 
        gacctctgc tctgagaat aatatttcg gcgacggag 
        cgcgcggct aaaggggag aacaaagcc cccgagatg 
        tttctggag ggcgagccg gcgccgtgg gaggacttc 
        accgttcgg aaaatctcc acgttggtg ggaggcgat 
        ccgattgat cttttagac acgagtgat ctctccgca 
        cttgaaact tcagctgat atcagcaag gagttggcc 
        aacggcatc gactatttc ctcgatgcc gatgtcgtg 
        ccgtacgac actaaaatt caaattcgg ggctcgaaa 
        atccccgcg tggttcccc gggtatgtg tcagctttt 
        ccgcactta cctgaactt gttaaagtg accaaaagt 
        ccgagtgag gtacttcac gcggcttgg aaagctgtc 
        caaccgaaa gttttcccg gacgaacct ctcttaatt 
        gtctaa
      mRNA sequence
      • >TC012586 RA
        aatctcaaa cagagagtg ggcttaacc ccagaagtg 
        gatcaagcg agctgcgct ccgccctgt caaattttt 
        cgcaacaac ccacaaaat gtcccgtgt tcctcgact 
        taaaacgga aacctccca gcgtgtggt cccagaccc 
        cgcttcatc ccgactccc tcgcggagt ttcccgtgt 
        gactgaaaa gtcccagcg caatccccg gtcgggagt 
        cgggcgtca gctcggagt tgtgaaacc tgaagggac 
        tccgcaacc agggccctc gagattcca acaaggcgt 
        cttcaagca aaccttggc caagtcagt ggcggaccc 
        tgggaagaa ggtgcggac ggtgtctgc ctcacactc 
        acagattaa accccccaa agtgcaaac gaagcgagg 
        ggaggaatg tgtggtgca gcagatgtc gctggagac 
        gacggtgca gaggtgccc cagggcgga gtacatcgg 
        caagaccat gcgaggcgt cgacgtggc cccctcggg 
        ggtggcgcg ccagctgga actcacctc gcaacagat 
        cgacccgac acgtggaac ggaatcggc cggtgtcaa 
        cgaaactgt ttcggtcac tagcacgct ctcgattac 
        ttcgggagg gaagcagtt gaagaaaac ttacaagaa 
        gcagaccgc tattgcctc gaaattggc caaaaagcc 
        gaatttgaa cccgcacca catcaatcg gctggttaa 
        ggaggagac cgaagacgt ggaaaacac cttgaagat 
        ggtgcctcc gagtctagt ctcctcgtc catcgacaa 
        gtttccttt gaagaaaaa gttgcaaaa gttgcggct 
        taacgcgga gcctgtgag tcttttaac tcagtcggt 
        catccatcc tgcgcccac tgctgacga ggtcgatgc 
        cgaagcgcc cccggagct gatggccac ggtcatgga 
        tgccgtcga aaagggggc gacgccgga ctcggcccc 
        taaccagat tttcaacag tctcttccc cgaagtgca 
        cgaggcgcg gattagggt cagttcggt ggctcccgg 
        gcctttgga ggatgttgt cgtccccac ggatcccgc 
        cgtgagggt ggtcgacga ggctctgtc ttccgaaat 
        ggaggagtt tattgcttc cgaaatcca gaacaagca 
        agagttgcc cacggatga cctctgctc tgagaataa 
        tatttcggc gacggagcg cgcggctaa aggggagaa 
        caaagcccc cgagatgtt tctggaggg cgagccggc 
        gccgtggga ggacttcac cgttcggaa aatctccac 
        gttggtggg aggcgatcc gattgatct tttagacac 
        gagtgatct ctccgcact tgaaacttc agctgatat 
        cagcaagga gttggccaa cggcatcga ctatttcct 
        cgatgccga tgtcgtgcc gtacgacac taaaattca 
        aattcgggg ctcgaaaat ccccgcgtg gttccccgg 
        gtatgtgtc agcttttcc gcacttacc tgaacttgt 
        taaagtgac caaaagtcc gagtgaggt acttcacgc 
        ggcttggaa agctgtcca accgaaagt tttcccgga 
        cgaacctct cttaattgt ctaacatga aaaacgcaa 
        taaacgaga ttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY