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Gene information
General information
TC001718 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8258828..8267950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001718
    iBeetle screen iB_10975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001718 RA
      MMESNHDDL NGKHRKIPD RNNKRIFDV RLTEDTTCG 
      IWVLKGPWL QRFANKKAY VFLYGLLGC VFSAAYAYF 
      NGTITTLEK RFKIPSRTT GIITVGNDL SQLFVSVIL 
      SYYAGRGHR PRWMALGMY TVVLFCLLT ALPHFLCGA 
      GSDALTLTV EYRTHNSSV EPLRKKALC HHKNSTTSD 
      CEIEGGNYS PQLILFIAQ FISGIGGSL YYTLGVSYM 
      DDNIKKSKT PALFSFSYF IRMLGPAVG YGLASICLK 
      LYISPSLTP IIDNNDPRW LGAWWLGWI ILALVIFIF 
      ASLLALFPK TLPRAAVRR ELLKKQICK KNKKCEPEP 
      ELPTSFRDM LVTFKRLVS NPTLMLNNF AAIFYFLGY 
      MPYWIFLPK YIETQYRQS ASASSLITG SAGLVFSAI 
      GILLSGLII SKYKPRARY LAAWNVIVG AISVLGIVS 
      YAYLGCAEN DNRAPLLPN GELNPVTEC NSHCFCDYV 
      KYNPVCSQD GTTTFISAC HAGCRHSKK INGTEIFSD 
      CSCILSNRN KNINSTVHE GGFATPGNC SVDCLSKFY 
      IFLIVVCVL KLSGATGRA SNFLVTVRC VEEKDKPVA 
      MGFGLMLMS LCAFVPSPI LFGIILGKT NNRER
    CDS sequence
    • >TC001718 RA
      atgatggag tcaaatcat gatgatctc aatggaaaa 
      catcgaaag atacctgac agaaacaat aagcgtata 
      tttgacgtt agattaacc gaagacacc acttgtggt 
      atttgggtt ttgaaagga ccttggctc caaagattc 
      gccaataag aaagcctat gtgttcctg tatggatta 
      ctcggatgc gtattttca gcagcgtat gcctatttt 
      aatggtact attacaaca ctggaaaaa cgatttaaa 
      ataccgagc aggacgaca ggcataatt acggttgga 
      aatgactta agtcagtta tttgtctcc gtaattctg 
      agttactac gccgggaga ggacataga ccgaggtgg 
      atggctctg gggatgtat actgtggtc cttttttgt 
      ctattaaca gcattgcct cactttctt tgtggcgct 
      ggatccgac gctttgacg ctcaccgtg gaataccga 
      actcacaat tcctctgtg gaacctttg cgaaagaaa 
      gctttatgt catcacaaa aattcgacc acatctgac 
      tgcgaaata gaaggcggg aattattcg cctcagtta 
      attctcttt attgctcag tttatatcc ggaatcgga 
      ggatcactc tactacact ctcggtgtc tcctacatg 
      gacgacaac atcaagaaa tccaaaaca ccagcttta 
      ttcagtttt tcatacttt attcgaatg ttgggccca 
      gcggttggt tacggtttg gcttcaatt tgcctaaaa 
      ttgtacatt tcaccaagt ctgacccca attatcgac 
      aacaacgac ccgcgatgg ttaggggcc tggtggctt 
      ggttggata attctagcc ttggttatt ttcatattt 
      gcatcgttg ctagccctg tttccgaaa actcttccg 
      cgtgcagct gtacgaaga gagttactc aagaaacaa 
      atatgtaaa aagaataag aaatgtgag cctgaaccc 
      gaattacca acgtctttt agagatatg ttggtaact 
      tttaaacgg ttagtgtcc aatccaacc ctgatgttg 
      aacaatttt gcggcaatt ttctatttt ttgggttac 
      atgccgtat tggattttt ttaccaaag tatatagaa 
      acgcaatat cgtcaatca gcgtctgct tctagtttg 
      ataacagga agtgctggt cttgtattt tctgcgatt 
      ggtattcta ttgtcagga cttattatt tccaagtat 
      aagccacgt gcacgatac ttagcggct tggaacgta 
      attgttgga gcgatttca gttcttggt attgtttca 
      tatgcttac ttaggatgt gctgaaaat gacaacaga 
      gctccttta ctaccaaac ggagaatta aatccagta 
      actgaatgt aattcgcat tgcttttgt gattacgta 
      aaatacaat cctgtgtgt tcgcaagac gggacaacg 
      acttttata tcagcgtgt catgctggc tgtagacat 
      tcgaagaaa atcaatggc acggagatt ttttccgat 
      tgttcctgc attttgtcg aatcggaac aaaaatatt 
      aattctaca gtgcacgaa ggcgggttt gcaactccc 
      ggaaactgc tcggtggat tgtttatcg aaattttat 
      atttttctg attgttgtc tgtgttctg aagctcagt 
      ggggcgact gggagggca tcgaatttt ttagtcaca 
      gtgaggtgt gtagaggag aaagataaa cctgtagcg 
      atgggtttc ggtttgatg ctaatgagt ctttgtgct 
      ttcgttcct tcacccatc cttttcgga ataatctta 
      ggtaagaca aataataga gagcgatag
    mRNA sequence
    • >TC001718 RA
      atagtgttg tttgtaaac gttgcggac aaccgacag 
      aagaattat tacatcaat aaaagatgt cacttgata 
      gtaatgtcc ttagttatt aaggttcaa gtgactgtg 
      ttgtatttt cttaagaat gatggagtc aaatcatga 
      tgatctcaa tggaaaaca tcgaaagat acctgacag 
      aaacaataa gcgtatatt tgacgttag attaaccga 
      agacaccac ttgtggtat ttgggtttt gaaaggacc 
      ttggctcca aagattcgc caataagaa agcctatgt 
      gttcctgta tggattact cggatgcgt attttcagc 
      agcgtatgc ctattttaa tggtactat tacaacact 
      ggaaaaacg atttaaaat accgagcag gacgacagg 
      cataattac ggttggaaa tgacttaag tcagttatt 
      tgtctccgt aattctgag ttactacgc cgggagagg 
      acatagacc gaggtggat ggctctggg gatgtatac 
      tgtggtcct tttttgtct attaacagc attgcctca 
      ctttctttg tggcgctgg atccgacgc tttgacgct 
      caccgtgga ataccgaac tcacaattc ctctgtgga 
      acctttgcg aaagaaagc tttatgtca tcacaaaaa 
      ttcgaccac atctgactg cgaaataga aggcgggaa 
      ttattcgcc tcagttaat tctctttat tgctcagtt 
      tatatccgg aatcggagg atcactcta ctacactct 
      cggtgtctc ctacatgga cgacaacat caagaaatc 
      caaaacacc agctttatt cagtttttc atactttat 
      tcgaatgtt gggcccagc ggttggtta cggtttggc 
      ttcaatttg cctaaaatt gtacatttc accaagtct 
      gaccccaat tatcgacaa caacgaccc gcgatggtt 
      aggggcctg gtggcttgg ttggataat tctagcctt 
      ggttatttt catatttgc atcgttgct agccctgtt 
      tccgaaaac tcttccgcg tgcagctgt acgaagaga 
      gttactcaa gaaacaaat atgtaaaaa gaataagaa 
      atgtgagcc tgaacccga attaccaac gtcttttag 
      agatatgtt ggtaacttt taaacggtt agtgtccaa 
      tccaaccct gatgttgaa caattttgc ggcaatttt 
      ctatttttt gggttacat gccgtattg gattttttt 
      accaaagta tatagaaac gcaatatcg tcaatcagc 
      gtctgcttc tagtttgat aacaggaag tgctggtct 
      tgtattttc tgcgattgg tattctatt gtcaggact 
      tattatttc caagtataa gccacgtgc acgatactt 
      agcggcttg gaacgtaat tgttggagc gatttcagt 
      tcttggtat tgtttcata tgcttactt aggatgtgc 
      tgaaaatga caacagagc tcctttact accaaacgg 
      agaattaaa tccagtaac tgaatgtaa ttcgcattg 
      cttttgtga ttacgtaaa atacaatcc tgtgtgttc 
      gcaagacgg gacaacgac ttttatatc agcgtgtca 
      tgctggctg tagacattc gaagaaaat caatggcac 
      ggagatttt ttccgattg ttcctgcat tttgtcgaa 
      tcggaacaa aaatattaa ttctacagt gcacgaagg 
      cgggtttgc aactcccgg aaactgctc ggtggattg 
      tttatcgaa attttatat ttttctgat tgttgtctg 
      tgttctgaa gctcagtgg ggcgactgg gagggcatc 
      gaatttttt agtcacagt gaggtgtgt agaggagaa 
      agataaacc tgtagcgat gggtttcgg tttgatgct 
      aatgagtct ttgtgcttt cgttccttc acccatcct 
      tttcggaat aatcttagg taagacaaa taatagaga 
      gcgatagaa cgttgtcac tttaggaat tctttccag 
      ataaggcct gtctggtgt ggggaaaga cttgtaccg 
      gaaatggaa actgttggt tatataatg gaaaaacct 
      taagataca caatgaatt tcacagctg cgacattcg 
      ttctgattg gaactctct tcgatgctg gcgtctggt 
      tttttgtca aaggattaa aaatatttg acgaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY