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Gene information
General information
TC007348 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5813207..5818156
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007348
    iBeetle screen iB_10969

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature complete fluorescence not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature random fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen cuticle ventral potentiallystructure thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen bristle potentially number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment sometimes potentiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment ventral not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007348 RA
      MLPRCYLFA KNVCQKAAV SSCEKNCSI TLNVAVRQL 
      YLLHRNTQI IPLNSHHTR QFTWTQNLL KKRDTGKQT 
      ADQGVDTNE VVSSKTTDK DKKDEPPTP VLKIIAPEI 
      QSNTNTKSK DKVVLDNVL EQSLDKKVA KPVISEPIK 
      KLKEKIIGI SENKNEEGK TQTKKRKRV DLSAQSLER 
      NFITPVRAM SDFLLKPSD LESLPKTKR RSPYENEPP 
      ITVYWRKDV EAKALSIWG SRENLQKEL IKRDIERKK 
      YQQNIFTMK RRLRDYRRE LGRSANVET DGGIMGRSG 
      KVVLTAIAI NGTNCLFKL FAWLYTGSH SMFSECIHS 
      LADTINQLI LAYGIHKSV QVADSYHPY GYTNMKYVA 
      SLISGVGIF CVGTGLSLY HGISGLVNP QPIDDFFWA 
      YFILGGSVV SEGATWLVA YNSIRRGAR NINMSVKDY 
      ILRSQDPSV NVVLLEDFA AVVGVIVAA GCMGLSSVT 
      NSSIPDAIG SLLVGCILG SVASFIIYT NVAALVGRS 
      IPEESLDKI NTELESDVM VRAIHDVKG IDMGNYLVR 
      YKAELDFDG RELTRSYLD KQDLNELLE EARRLENID 
      QFEEFMLKH GENIVDMMG GEIDRIEMK LRKKHPEIR 
      HCDLEIL
    CDS sequence
    • >TC007348 RA
      atgctgcct cgctgttac ttattcgcg aaaaatgtg 
      tgtcagaaa gctgccgtt tccagctgt gagaagaac 
      tgctccatt acattaaat gtggcagtt agacaactt 
      tatttatta cacagaaat actcaaata attccctta 
      aattcacac cacactcga cagtttaca tggacacaa 
      aacttgctt aaaaaacgg gatacaggt aaacaaaca 
      gctgaccag ggagtagac actaacgaa gtagtaagt 
      agtaaaacg acagataaa gataagaag gatgaacca 
      ccaacgccc gtacttaaa ataatagct ccagaaatc 
      cagtctaac actaacact aaatctaaa gataaagtc 
      gtactagat aatgtcttg gaacaaagc ttagacaaa 
      aaagtagcc aagcccgtg atatccgaa cccataaaa 
      aagctaaaa gagaaaata attggtatt agcgaaaac 
      aaaaacgaa gaaggaaaa acacaaacc aaaaagcgg 
      aaacgcgtt gatttatcc gcacaatcg ctcgagcgc 
      aatttcata actcctgta cgtgccatg tccgacttt 
      ttgctgaaa ccatccgat ttggaatca ttgcctaag 
      acaaaacga cgttcccct tacgaaaat gaaccacca 
      attacagtc tactggcgc aaagacgta gaagctaaa 
      gctttaagt atctgggga tcaagggaa aatctccag 
      aaagaactg attaagcga gatatcgag agaaagaaa 
      taccaacaa aatattttt acaatgaag aggcgattg 
      cgtgattat agacgcgaa ctgggtcgc tcggctaat 
      gtggagact gacgggggc attatgggg cggtcgggg 
      aaagtggtc ttgacagca attgccatc aatgggaca 
      aattgtctc tttaaacta ttcgcctgg ttgtacaca 
      gggtcacac agcatgttt tctgaatgt atacactcg 
      ttggctgat actataaat cagctaatt ttggcctac 
      ggcatccat aaatctgtc caggttgcc gactcttat 
      cacccatat gggtacaca aacatgaaa tacgtggcg 
      tccctcatt tccggggtt gggattttt tgcgtaggg 
      acgggtttg tctttatac cacggtatt tcaggtcta 
      gtcaatcct caacccatt gatgacttt ttctgggct 
      tattttatt ttggggggc tcggtagtt tccgagggc 
      gcaacctgg ttagttgcc tacaattcc atccgcaga 
      ggtgcccgt aatattaac atgtcggtg aaggattac 
      attttgcgc tcgcaggat ccaagcgtc aatgttgtt 
      cttctggaa gattttgcg gctgttgtt ggtgtgatc 
      gtggcagct gggtgcatg ggtctgtcc tctgtgacc 
      aatagttct atccctgac gctattggg tcgctgctt 
      gttggctgt attttagga tcagttgca tcgtttatt 
      atttacacg aatgttgct gcacttgtt ggacggtcc 
      ataccagag gaaagtttg gataaaatt aacactgaa 
      ctggagagt gatgttatg gtacgggcg atccatgat 
      gttaaaggt atcgatatg ggcaattat ttggtgcgg 
      tataaagcc gagctggat tttgacggt cgggaatta 
      acgaggtct tatctggac aagcaagac ctcaacgaa 
      ttgcttgaa gaggctcga cgtttggaa aatattgat 
      caatttgag gagtttatg ttgaaacat ggcgaaaat 
      attgttgat atgatggga ggggaaatt gataggatt 
      gaaatgaaa ttaagaaaa aaacatccc gaaattaga 
      cattgtgat ctagaaatt ctttaa
    mRNA sequence
    • >TC007348 RA
      ccaatagct tggctgtat tttacaatc aggcaagac 
      aggaaattt taaatgtcg cctaattgt tgaacgtga 
      agttgtcat tgcgtcaaa aaatttgtc aagttacaa 
      aaagccaaa aaagtgtaa aaaagacct gaaggcgca 
      cgaaatctg tgctaaagt gtgacatct ttagcttcc 
      aatttaata aaattggag taatctttg agtaattct 
      tcaaacaag gttaaattc gatcattcg gtgcttttt 
      tttggttat gtacacctg tcaaacatt ccgttttta 
      aacatataa tttttgtcc gctgtataa attgttaaa 
      aatgctgcc tcgctgtta cttattcgc gaaaaatgt 
      gtgtcagaa agctgccgt ttccagctg tgagaagaa 
      ctgctccat tacattaaa tgtggcagt tagacaact 
      ttatttatt acacagaaa tactcaaat aattccctt 
      aaattcaca ccacactcg acagtttac atggacaca 
      aaacttgct taaaaaacg ggatacagg taaacaaac 
      agctgacca gggagtaga cactaacga agtagtaag 
      tagtaaaac gacagataa agataagaa ggatgaacc 
      accaacgcc cgtacttaa aataatagc tccagaaat 
      ccagtctaa cactaacac taaatctaa agataaagt 
      cgtactaga taatgtctt ggaacaaag cttagacaa 
      aaaagtagc caagcccgt gatatccga acccataaa 
      aaagctaaa agagaaaat aattggtat tagcgaaaa 
      caaaaacga agaaggaaa aacacaaac caaaaagcg 
      gaaacgcgt tgatttatc cgcacaatc gctcgagcg 
      caatttcat aactcctgt acgtgccat gtccgactt 
      tttgctgaa accatccga tttggaatc attgcctaa 
      gacaaaacg acgttcccc ttacgaaaa tgaaccacc 
      aattacagt ctactggcg caaagacgt agaagctaa 
      agctttaag tatctgggg atcaaggga aaatctcca 
      gaaagaact gattaagcg agatatcga gagaaagaa 
      ataccaaca aaatatttt tacaatgaa gaggcgatt 
      gcgtgatta tagacgcga actgggtcg ctcggctaa 
      tgtggagac tgacggggg cattatggg gcggtcggg 
      gaaagtggt cttgacagc aattgccat caatgggac 
      aaattgtct ctttaaact attcgcctg gttgtacac 
      agggtcaca cagcatgtt ttctgaatg tatacactc 
      gttggctga tactataaa tcagctaat tttggccta 
      cggcatcca taaatctgt ccaggttgc cgactctta 
      tcacccata tgggtacac aaacatgaa atacgtggc 
      gtccctcat ttccggggt tgggatttt ttgcgtagg 
      gacgggttt gtctttata ccacggtat ttcaggtct 
      agtcaatcc tcaacccat tgatgactt tttctgggc 
      ttattttat tttgggggg ctcggtagt ttccgaggg 
      cgcaacctg gttagttgc ctacaattc catccgcag 
      aggtgcccg taatattaa catgtcggt gaaggatta 
      cattttgcg ctcgcagga tccaagcgt caatgttgt 
      tcttctgga agattttgc ggctgttgt tggtgtgat 
      cgtggcagc tgggtgcat gggtctgtc ctctgtgac 
      caatagttc tatccctga cgctattgg gtcgctgct 
      tgttggctg tattttagg atcagttgc atcgtttat 
      tatttacac gaatgttgc tgcacttgt tggacggtc 
      cataccaga ggaaagttt ggataaaat taacactga 
      actggagag tgatgttat ggtacgggc gatccatga 
      tgttaaagg tatcgatat gggcaatta tttggtgcg 
      gtataaagc cgagctgga ttttgacgg tcgggaatt 
      aacgaggtc ttatctgga caagcaaga cctcaacga 
      attgcttga agaggctcg acgtttgga aaatattga 
      tcaatttga ggagtttat gttgaaaca tggcgaaaa 
      tattgttga tatgatggg aggggaaat tgataggat 
      tgaaatgaa attaagaaa aaaacatcc cgaaattag 
      acattgtga tctagaaat tctttaagt atgcaaata 
      ataattgtt aaataaata ttacacaga atttgtttt 
      tttttttgt cagaaaaaa ggataagca gtatttaca 
      tttgtgaca aatggcgta accattatt tttatattt 
      tattttact tccgaagct ttgtcagtc tgcctctgt 
      ggtgttaat aaaaactta aaaaattg
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