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Gene information
General information
TC000531 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10137372..10140229
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000531
    iBeetle screen iB_10968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 2

    egg chamber potentiallydegenerated - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000531 RA
      MIKRGSVEL LDMDSNSYG KNLTLDTFR SDSKSNLIN 
      SKQETTSWG KTSPSKSYC SSSSTSTEN VVSTLERKK 
      ATSFESNVP NRPDDPPLL QGEKVTGQA RDVTYLCPY 
      YGPARGVLT VTNYRLYFR SADKDTPNI IDVPLGVVS 
      RIEKVGGAS SRGENAYGI EVFCKDMRN LRFAHKQEN 
      HSRRDVFEK LQQFAFPLS HKIKLFAFE YSETFPENG 
      WAIYEPIAE LKRMGVNND MWKISRINE QYDICDSYP 
      AVWAIPAQA TEEDIKSVA SYRSRARIP VLSWIHPES 
      QATITRCSQ PLVGVSGKR CREDERYIQ LIMDANAQS 
      HKMFIMDAR PSANAIANK AKGGGYESE DAYQNAELV 
      FLDIHNIHV MRESLRKLK ELCYPNIEE TKWLSGVES 
      TYWLKHIKC ILAGAVRIV DKVENHKTS VLVHCSDGW 
      DRTAQLTAL PMLMLDPYY RTIKGFEVL IEKEWLSFG 
      HKFQQRIGH GDDHHSDAD RSPVFLQFI DCVWQITQQ 
      FPNAFEFND YFLITILDH LYSCRFGTF LCNSERERV 
      QEKLKEQTV SLWSYTNSH LDLYKNPLY WANNNQQRV 
      LIPIASIRH IKLWKAYYC RWNPSMRTQ DPVYQRIRE 
      LLILKEQLE NTVEQCRRE QQSRMARSN VTPA
    CDS sequence
    • >TC000531 RA
      atgataaaa agaggaagt gtcgagtta ttagacatg 
      gacagtaat agctacggt aaaaatcta acattggat 
      accttccgg tcggattcc aaatctaac ttaataaat 
      tcaaaacaa gaaactact agctggggt aagacctcg 
      cccagcaag agctactgc tcctccagt tcaacatca 
      acggaaaat gtagtttcc accctcgag cggaaaaaa 
      gcaacatca ttcgaaagc aacgtcccc aaccggccc 
      gacgacccc cctttactc cagggtgaa aaagtaaca 
      ggccaagca cgggacgtc acttacctt tgcccctat 
      tacggcccc gcaagggga gtcttgacc gtaaccaac 
      tataggttg tatttcaga tcggcagat aaagacacc 
      cctaacatt atcgacgtg ccattgggt gtggtgtca 
      cgtattgag aaagtcggg ggcgcctcc tcgcgtggg 
      gagaacgcc tatggcatc gaagtgttc tgcaaagac 
      atgcgaaac ttgcgtttc gcccataaa caggaaaac 
      cactcgcgc cgcgacgtt ttcgaaaag ttgcaacaa 
      ttcgccttc cccctgtcc cacaaaatc aagctgttc 
      gcgttcgaa tactctgag acttttccc gagaacggg 
      tgggcgatt tatgagcca atcgcggag ctcaagaga 
      atgggggtc aataacgac atgtggaaa atatcgcga 
      ataaatgag cagtacgac atttgcgat agctatccg 
      gctgtttgg gcgattccg gcacaagcc actgaggag 
      gatattaaa tctgtggcg agctatcgc agccgcgct 
      aggattcct gtccttagt tggattcac cccgaatcg 
      caggccacc atcaccagg tgttcgcaa cctttggtt 
      ggggtgagt ggtaaaagg tgccgcgag gacgagaga 
      tacatccaa ctgataatg gatgcgaac gctcagtcg 
      cataaaatg ttcatcatg gatgcgagg cctagcgct 
      aacgcgatc gccaataag gcaaaaggg ggcgggtac 
      gagtctgaa gacgcctac cagaacgca gagctcgtg 
      tttctggac attcacaac attcacgta atgcgggag 
      tctttgcga aagctcaaa gaactgtgc tatcccaac 
      attgaggaa acgaagtgg ctgtcaggg gtcgagtca 
      acctattgg ctgaagcac atcaagtgt attttagcc 
      ggggctgtc cgaatcgtt gataaggtt gaaaatcac 
      aaaacttca gtgttggtg cactgttcg gacggctgg 
      gaccgcaca gcgcaattg acggctttg ccaatgctg 
      atgctagac ccttactat cgcacgatc aaaggtttt 
      gaagttttg attgagaaa gaatggctc tcatttggc 
      cacaaattc caacagcgt atcggacac ggtgatgac 
      catcattcg gatgcagac aggtcacct gtttttctc 
      cagtttatc gactgcgtg tggcagata acgcaacag 
      tttcccaac gctttcgag ttcaatgat tacttttta 
      attacgatt cttgatcat ttgtattcg tgtcggttt 
      ggcacgttt ttgtgtaac agtgaacgg gaaagggtg 
      caagagaaa cttaaagaa cagactgtc tctttgtgg 
      tcttacacc aacagtcat ctagacttg tacaaaaat 
      ccgctctat tgggccaac aataatcag cagagagta 
      ttgattccg attgcaagt attagacat atcaagctg 
      tggaaggct tactattgc aggtggaac cccagcatg 
      cgaacacag gacccagtt taccagcga ataagagag 
      ttgctgata ctgaaggag cagctggaa aataccgta 
      gaacagtgc cgcagggag caacagtca cggatggct 
      cgaagtaac gtgacgcca gcatag
    mRNA sequence
    • >TC000531 RA
      agctgggtt tttgacagg aacaagtga tttgacagc 
      aattaatga tgaaaattg aaaaataaa actaaaata 
      atcaaaaga atgataaaa agaggaagt gtcgagtta 
      ttagacatg gacagtaat agctacggt aaaaatcta 
      acattggat accttccgg tcggattcc aaatctaac 
      ttaataaat tcaaaacaa gaaactact agctggggt 
      aagacctcg cccagcaag agctactgc tcctccagt 
      tcaacatca acggaaaat gtagtttcc accctcgag 
      cggaaaaaa gcaacatca ttcgaaagc aacgtcccc 
      aaccggccc gacgacccc cctttactc cagggtgaa 
      aaagtaaca ggccaagca cgggacgtc acttacctt 
      tgcccctat tacggcccc gcaagggga gtcttgacc 
      gtaaccaac tataggttg tatttcaga tcggcagat 
      aaagacacc cctaacatt atcgacgtg ccattgggt 
      gtggtgtca cgtattgag aaagtcggg ggcgcctcc 
      tcgcgtggg gagaacgcc tatggcatc gaagtgttc 
      tgcaaagac atgcgaaac ttgcgtttc gcccataaa 
      caggaaaac cactcgcgc cgcgacgtt ttcgaaaag 
      ttgcaacaa ttcgccttc cccctgtcc cacaaaatc 
      aagctgttc gcgttcgaa tactctgag acttttccc 
      gagaacggg tgggcgatt tatgagcca atcgcggag 
      ctcaagaga atgggggtc aataacgac atgtggaaa 
      atatcgcga ataaatgag cagtacgac atttgcgat 
      agctatccg gctgtttgg gcgattccg gcacaagcc 
      actgaggag gatattaaa tctgtggcg agctatcgc 
      agccgcgct aggattcct gtccttagt tggattcac 
      cccgaatcg caggccacc atcaccagg tgttcgcaa 
      cctttggtt ggggtgagt ggtaaaagg tgccgcgag 
      gacgagaga tacatccaa ctgataatg gatgcgaac 
      gctcagtcg cataaaatg ttcatcatg gatgcgagg 
      cctagcgct aacgcgatc gccaataag gcaaaaggg 
      ggcgggtac gagtctgaa gacgcctac cagaacgca 
      gagctcgtg tttctggac attcacaac attcacgta 
      atgcgggag tctttgcga aagctcaaa gaactgtgc 
      tatcccaac attgaggaa acgaagtgg ctgtcaggg 
      gtcgagtca acctattgg ctgaagcac atcaagtgt 
      attttagcc ggggctgtc cgaatcgtt gataaggtt 
      gaaaatcac aaaacttca gtgttggtg cactgttcg 
      gacggctgg gaccgcaca gcgcaattg acggctttg 
      ccaatgctg atgctagac ccttactat cgcacgatc 
      aaaggtttt gaagttttg attgagaaa gaatggctc 
      tcatttggc cacaaattc caacagcgt atcggacac 
      ggtgatgac catcattcg gatgcagac aggtcacct 
      gtttttctc cagtttatc gactgcgtg tggcagata 
      acgcaacag tttcccaac gctttcgag ttcaatgat 
      tacttttta attacgatt cttgatcat ttgtattcg 
      tgtcggttt ggcacgttt ttgtgtaac agtgaacgg 
      gaaagggtg caagagaaa cttaaagaa cagactgtc 
      tctttgtgg tcttacacc aacagtcat ctagacttg 
      tacaaaaat ccgctctat tgggccaac aataatcag 
      cagagagta ttgattccg attgcaagt attagacat 
      atcaagctg tggaaggct tactattgc aggtggaac 
      cccagcatg cgaacacag gacccagtt taccagcga 
      ataagagag ttgctgata ctgaaggag cagctggaa 
      aataccgta gaacagtgc cgcagggag caacagtca 
      cggatggct cgaagtaac gtgacgcca gcatagtcc 
      ttgtgtaac caaattttc tgtaaatac tagggctaa 
      aagcatatt atgtatttc atttgtatt ctcgtaaaa 
      taatctgac catgtaatt ttaagttgc aaattttta 
      ctaattata tattaatct acaatgcaa tacccgaat 
      ataattaat aaatttttt gtccgtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY