Status
Please wait ...
Gene information
General information
TC000407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11871432..11872879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000407
    iBeetle screen iB_10966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000407 RA
      MEEPIGAQY AVHVALQTL KERCKNLQE MVTTLEEEN 
      LHLRGQLSK SEQSSVSLS DFDKLKAQV AELTENKLQ 
      LLNKVKMVT NENQELWNK LSRLSHVNH NLGTQLSKI 
      NDSLVQYTS KNKETHSPL IRSKTFTQD EPHTKVLQK 
      NIEENGKIC AELEEISLK IINGFAQDK SELDSLYSE 
      IRGAQWGEN IISESCGFQ YDDYESNII ENFNSILEE 
      LKTTKLTVA NQRVALQLA LSKLNNIQE IKRKKVCDN 
      KLAEKKEMV DKSISTVNL CETRESPKT HALPETSLQ 
      DPPETTDMI CPICCKNFN GGLSFQVFQ DHVEKHFTE 
      ETFELL
    CDS sequence
    • >TC000407 RA
      atggaggag cctattggg gcccagtat gccgttcat 
      gtggctttg caaacgctc aaagagcgc tgtaagaac 
      ctgcaagaa atggtgacg accctcgag gaagaaaac 
      ctccatttg cgaggccag cttagtaag agcgagcaa 
      agcagtgtc tcactgtcc gatttcgat aaattaaag 
      gcacaggtg gccgaactg accgaaaat aagctccag 
      ttattgaat aaagtcaag atggtgacg aacgaaaac 
      caggaattg tggaataag ttaagtagg ttgagtcat 
      gtaaatcat aatcttggg acacagttg agtaaaatc 
      aacgattcg ttggtgcaa tacacctcg aaaaataag 
      gagacgcat tcgcctttg atccgctcg aaaaccttc 
      acgcaggac gagccgcat acgaaagtg ctccagaag 
      aatattgaa gaaaatggg aagatttgt gcagagttg 
      gaggagatt tctctgaaa ataatcaat gggttcgct 
      caggataaa agcgaactt gactcgctc tattcggag 
      atcagggga gcgcagtgg ggcgagaat attatatca 
      gagtcctgt gggtttcag tatgacgac tatgaaagc 
      aacatcata gagaacttt aacagtatt ttggaagaa 
      ttaaagacg actaagctg accgtagct aaccagaga 
      gtggccttg caattagcc ttaagtaaa ttaaacaat 
      atacaagag attaaacga aagaaggtc tgtgataat 
      aaactagcg gagaaaaaa gaaatggta gataagagt 
      atatcaacg gttaattta tgcgaaacg cgcgaatct 
      ccaaagact catgctctt ccagaaacc agccttcaa 
      gaccctccg gaaaccaca gacatgata tgccccata 
      tgttgcaag aatttcaac ggtggcttg agctttcaa 
      gttttccaa gaccacgtt gagaaacat tttacagag 
      gaaactttt gaattattg tga
    mRNA sequence
    • >TC000407 RA
      gtttttgcc aaatttctt gaataccac aaacacaca 
      caaaaacag ggacttctc cctgtaatc agtcggttc 
      tttgttcgt ttcaccgtg ctaaagttt cccgtggcg 
      taaagtttg gttatgtca taaagcctc aaagagcat 
      ggaggagcc tattggggc ccagtatgc cgttcatgt 
      ggctttgca aacgctcaa agagcgctg taagaacct 
      gcaagaaat ggtgacgac cctcgagga agaaaacct 
      ccatttgcg aggccagct tagtaagag cgagcaaag 
      cagtgtctc actgtccga tttcgataa attaaaggc 
      acaggtggc cgaactgac cgaaaataa gctccagtt 
      attgaataa agtcaagat ggtgacgaa cgaaaacca 
      ggaattgtg gaataagtt aagtaggtt gagtcatgt 
      aaatcataa tcttgggac acagttgag taaaatcaa 
      cgattcgtt ggtgcaata cacctcgaa aaataagga 
      gacgcattc gcctttgat ccgctcgaa aaccttcac 
      gcaggacga gccgcatac gaaagtgct ccagaagaa 
      tattgaaga aaatgggaa gatttgtgc agagttgga 
      ggagatttc tctgaaaat aatcaatgg gttcgctca 
      ggataaaag cgaacttga ctcgctcta ttcggagat 
      caggggagc gcagtgggg cgagaatat tatatcaga 
      gtcctgtgg gtttcagta tgacgacta tgaaagcaa 
      catcataga gaactttaa cagtatttt ggaagaatt 
      aaagacgac taagctgac cgtagctaa ccagagagt 
      ggccttgca attagcctt aagtaaatt aaacaatat 
      acaagagat taaacgaaa gaaggtctg tgataataa 
      actagcgga gaaaaaaga aatggtaga taagagtat 
      atcaacggt taatttatg cgaaacgcg cgaatctcc 
      aaagactca tgctcttcc agaaaccag ccttcaaga 
      ccctccgga aaccacaga catgatatg ccccatatg 
      ttgcaagaa tttcaacgg tggcttgag ctttcaagt 
      tttccaaga ccacgttga gaaacattt tacagagga 
      aacttttga attattgtg atagagttc ataacttat 
      ttgtttgaa agtattata ttttctgtt ttaatcgat 
      tggatgtta aggttcgca aataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY