Status
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Gene information
General information
TC014664 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13157556..13164080
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014664
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random pattern not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature potentially rounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs pretarsus bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    posterior terminus segment potentially size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014664 RA
      MWKIVIFGF VIACVRCQG FYKVVGPRA VRPNSEYHV 
      AVSIQQTSE PTTVRATLQ GISPNGSIY TSDDRVEVQ 
      PYTSRILRL EVGDVDSGD YKLVVSGKG GVDFYSDYP 
      VEFVEKSYS VFIQTDRAV YKPGSKVLF RAVVLNSQL 
      KPAAEVRNE LLHIFVTDG QGNRIKEWK GIQALRGVF 
      TGEVKLSES PVLGNWNIS VKIHGQTFS KSIEVAEYI 
      LPKFIVNIQ APKHMTFKE NVLVANIQT QYNYGKKVK 
      GEATVTVYP TIFSGVIQP IFQNPIRKV IPIEGSATV 
      TFDIAKELK LTDEYERVV MVDVTVEEA STGRRQNNS 
      VEVHLHKYN YKMDLIKTA DYFKPGLKY TAYVKVSSH 
      EGTPLRSEN REVTVRYGY SRADEVYVT EKHRLDKNG 
      VAKLEYYTP VNVTNTTAL RIEAQYQDL KERISPIPA 
      AVSYSNTFL QVSLETERP IVNLDVEIL VNCTERLRY 
      ISYVLMGRG DVLNANTFQ VDNMHEYRF HFTATHAMV 
      PVAHLIVSY VRDDGELVG DALDIEVDG LLQNYMEIQ 
      VNPVETEPE LDIDIAIRA QPNSYVAIM AVDQNAVKM 
      RPGFDLTHS EVAEELKKY DPAQQSPYS MIMHDSKYH 
      FFWKPGAAN PHSAIYNSG ADLLTNSHV DRHQPTLED 
      IYLRPVFYG TSTVKPDRG FGLPLHTVT RPPLAGPYA 
      FSRIPKPVW NKPKVYLTE EIADTWLFT NFSSGYEGK 
      TSIRRKIPS SLNTWVVTG FSLDPIHGL GLTTTSKKV 
      KVSKSFVVT LDLPFSVQR REILAVPVV VYNYMDKDV 
      NAEVTLHNP EQKFEFAEV SNNVNSTRK VELYRRKKI 
      NIKRNSGTS VSFMIRPLK QDTIEIKVT ANSPKNQDV 
      AIKHLQVTT EGETEYYTK TVLIDLRNN PNYKKSINF 
      TIPQNMVTG SEKIEVSAV GDLLGPTMV HLENLIRLP 
      TGCGEQNLI HLMPNLIIL QYLRYTRQV TPTIQNEAL 
      DLLEKGYQQ QLSYKRKDG SFSAFGMRD EKSSVWVTA 
      YVALTLRQA KGHIYVDEK IIEGCLEWL ANIQGRNGS 
      FVEVGSVIY KEIQSREGN SLALTAFTL LAFIENQKY 
      ASTYSNTIN KGLDYIARY ISEQESIHT IALCSYTLQ 
      LARHPSKQS AFNLLDLRS KSRGNLKWW SKDVPSNEI 
      KNPWNKLPR SIDIETSAY GLLTFLEAN YFEDAIPVL 
      NWLLDQQNS LGGFTSSQD TFVGLWAIY KLVLKLATN 
      VNMQVEFTY GKDQRHNFN VNKNNAMIV QKLQLPKDI 
      REVNVTAQG KGLAVFRVS YEYNMNVTG PWPMFTLDP 
      QVDKNSNKD HLQVSICTG FVSRNLSET PESNMAVME 
      VNLPSGFTA DIDSLPSLE VSQNVQKVE TSNGLTRVT 
      LYFNNVSSV NEYCPTVSA FRTHKVANQ KPVSVIIFD 
      YYDSSRRAR QFYRSRTST LCDICEDED CGDICTSKA 
      NPQVGEDSQ EGKGSSAAL RSSWLGFLV VMFAIVQL
    CDS sequence
    • >TC014664 RA
      atgtggaaa attgtgatt tttggtttt gtgatcgcc 
      tgtgtcagg tgtcaaggg ttttacaag gtcgtgggg 
      ccaagagca gtccgcccc aacagcgaa taccatgtt 
      gccgtttca atccaacaa acatccgaa cctacgaca 
      gtccgagca actcttcaa ggcatttcc ccgaacggt 
      tcgatctac acaagcgac gaccgggta gaagttcag 
      ccttacacg tcccgaatc ctccggttg gaggtcgga 
      gacgtggac tccggcgac tacaaactg gtggtttcc 
      ggaaagggc ggggtggat ttttactcc gactatccg 
      gtagagttt gtggaaaaa agctactcg gtgtttatc 
      caaaccgat cgagcggtt tacaaaccc ggatccaaa 
      gtcttgttc cgggcggtt gtgctcaac tcgcagttg 
      aaaccggcg gcggaagtc cggaatgaa ctactccac 
      atttttgtg acggacggg caagggaat cggatcaag 
      gagtggaaa ggaatccag gcattgaga ggggtcttc 
      accggggaa gtgaaactg agcgagagc ccggttttg 
      ggaaactgg aatatttcg gtcaaaatc cacgggcaa 
      accttcagc aaatcaata gaagtcgct gaatatatt 
      ttaccaaaa ttcattgta aatattcaa gcgcccaaa 
      cacatgact ttcaaggaa aacgtcctt gttgccaat 
      atccaaacc caatacaat tatgggaaa aaagtcaaa 
      ggagaggcc actgtgacc gtttatccc acaattttc 
      tccggggtt attcaacct attttccag aacccgatc 
      cggaaagta atcccgatc gagggttcg gccactgtc 
      acattcgac attgccaaa gaactcaaa ctgactgac 
      gaatacgaa agggttgtc atggtcgat gtaacagtt 
      gaggaagct tcaacgggc cgccgacaa aacaattcg 
      gtcgaagtc catttgcat aaatacaat tataaaatg 
      gatctaatt aaaacagca gattatttc aaaccgggg 
      ctcaaatac acagcttat gtcaaggtt tcaagtcac 
      gaagggact cccctacga tcggaaaat agggaagtt 
      acggtcaga tacggctat tcgcgagct gatgaagtc 
      tatgttact gagaaacac aggttggat aaaaacggt 
      gttgcgaaa ttggagtat tacactccg gtgaatgtt 
      acaaacacc actgctctc aggattgag gctcagtac 
      caggacctc aaggagcgc atttcgcca attcctgct 
      gcggtttcg tacagtaac accttcctg caagtgtcg 
      ctcgaaacc gaaagaccg atcgtaaac ctcgatgtt 
      gaaatttta gtcaattgc actgaacgt ttgagatac 
      ataagctac gtcctgatg ggacgaggc gacgtcctg 
      aatgccaac accttccaa gttgacaac atgcacgaa 
      tatcggttt catttcacg gcaacacat gccatggtg 
      ccggttgcc caccttata gtgtcttac gtgcgtgac 
      gatggagaa ttggtggga gatgccctc gatattgaa 
      gttgatggg cttttgcaa aactacatg gagattcaa 
      gtcaatccg gtggaaacc gaacccgag cttgatatc 
      gacatagcg atcagagct cagcccaat tcgtacgtt 
      gcgataatg gcagttgac cagaacgcg gtcaaaatg 
      agacctggg tttgattta acccacagt gaagtagcg 
      gaagaattg aaaaaatac gacccggcc caacaatca 
      ccatattcc atgataatg cacgacagc aagtaccat 
      ttcttctgg aagccgggg gctgccaac ccacacagt 
      gccatttac aattcgggg gccgactta ttgaccaat 
      tctcacgtc gaccgccac caaccgact ttggaggat 
      atttacttg cggcccgtt ttctacggc actagcacc 
      gtcaaaccc gaccgcggc ttcggcctg cctctccac 
      accgtcacc aggccgccc ctggcaggt ccttacgcc 
      ttcagtcga atccctaag cccgtttgg aacaaaccg 
      aaggtttac ttgactgag gaaatcgcc gatacttgg 
      cttttcacc aacttctcg tcgggttac gaaggcaaa 
      acttcaatt cggcgaaaa ataccaagc tctctgaac 
      acttgggtc gtcactggc ttttccctg gaccccatt 
      catgggctg ggactgacc acaacttcc aagaaagtc 
      aaagtgtcg aaaagcttc gtcgtgacg cttgatttg 
      ccgttttcg gtgcaacgg cgagagatt ttagctgtg 
      cctgtggtc gtttacaat tatatggac aaggatgtg 
      aatgcggaa gtcacactt cacaacccg gaacaaaaa 
      ttcgagttt gctgaagtt tcaaataat gttaactcg 
      accagaaaa gtggagttg tacaggcgc aaaaaaatt 
      aacatcaag cgaaacagc ggcacgtca gtctcattc 
      atgatacgg ccgttgaaa caagacaca atcgagata 
      aaagtgacc gcaaacagt ccgaaaaac caggacgtg 
      gcaattaag catctccaa gtcacgact gaaggagag 
      acggaatat tacacaaaa accgtcctc attgattta 
      cgcaacaac cccaactac aaaaaatca atcaatttc 
      acaatcccc caaaacatg gtgaccggt tcggaaaaa 
      atcgaagtt tcagctgtt ggtgatctc ctaggcccg 
      actatggtc cacctcgaa aatttaatc agacttcca 
      acaggatgc ggtgaacaa aatttgatt catctcatg 
      ccgaattta atcattttg caatatctt cgctacacg 
      agacaagtt accccaacc attcaaaac gaagcgttg 
      gatttgctc gaaaagggt taccaacaa cagttgagt 
      tataaacgc aaggatggg tcgtttagt gcctttgga 
      atgagggat gagaagagc agtgtttgg gttacggct 
      tatgtggct cttacttta aggcaagcc aaaggccat 
      atttacgtt gacgagaaa attatcgaa ggttgtctc 
      gagtggttg gcgaatatt cagggccgg aatgggagt 
      tttgtcgaa gtggggtca gttatttac aaggagatt 
      cagagtcgg gaaggcaat agtttagcc ttgacggct 
      tttaccctc ttggcattt atcgaaaat cagaaatat 
      gcgtccact tatagcaat acaattaac aaaggattg 
      gattacatt gcacgttac atttctgaa caagaatca 
      atccatacg atagctttg tgtagctac actcttcaa 
      ttagcccgt cacccgtcg aaacaaagc gctttcaac 
      ttgcttgac ctccgttca aagagccga ggcaacttg 
      aaatggtgg tctaaggac gtcccaagt aacgagatt 
      aaaaatccc tggaacaaa ttaccgcgt tccatcgat 
      atcgagact tcagcctac ggtctttta accttcctt 
      gaggccaat tacttcgaa gacgcaatc cctgtcctg 
      aactggcta ctagaccag cagaacagt ctcggaggt 
      ttcacctca tcgcaggac actttcgtg ggcctttgg 
      gccatttat aaactagtc ctgaagttg gcaactaac 
      gtaaatatg caagttgag ttcacttac ggcaaagac 
      cagcgccac aatttcaac gttaataaa aacaacgca 
      atgattgtg caaaaactc caactaccg aaagacata 
      cgtgaagta aacgtgacg gctcagggc aaaggcttg 
      gccgttttt agggtctcg tatgagtat aatatgaac 
      gtaaccggg ccttggccc atgtttacg ctggaccca 
      caagtggac aaaaactcg aataaggac cacttacaa 
      gtgtcgatt tgcacagga tttgtgagt cgcaatttg 
      agtgaaacc cctgaaagc aacatggca gtgatggaa 
      gtgaacctc ccgtcgggt ttcaccgcc gatatcgac 
      tccttgcca agcctggaa gtgtcccaa aatgtgcaa 
      aaggtcgaa acgagcaac ggcctgact agagtcacc 
      ctctacttt aacaacgtg tcaagtgta aacgaatac 
      tgcccgaca gtgtccgct tttaggaca cataaagtc 
      gcaaaccaa aaacccgtc tcggttatt attttcgat 
      tattacgac agttcgaga cgtgccagg caattttac 
      cgaagccga acgtcaact ctgtgtgat atatgcgag 
      gatgaggac tgtggtgat atttgcacc tcaaaggcg 
      aacccacaa gtcggggag gacagtcag gaaggcaaa 
      ggcagctcg gctgctctg cgttcgagt tggttgggt 
      ttcctagtt gttatgttt gccattgtg caattgtga
    mRNA sequence
    • >TC014664 RA
      agacacaac tgcgatttt tgcggtgga aaaatacca 
      aactaggga aaaacaaca cacacaaaa atcgccagt 
      ggtcacaaa ttatcgaaa acgtgtctt ttgcgaaat 
      taaaatgac tttgtgatt gtgatgtgg aaaattgtg 
      atttttggt tttgtgatc gcctgtgtc aggtgtcaa 
      gggttttac aaggtcgtg gggccaaga gcagtccgc 
      cccaacagc gaataccat gttgccgtt tcaatccaa 
      caaacatcc gaacctacg acagtccga gcaactctt 
      caaggcatt tccccgaac ggttcgatc tacacaagc 
      gacgaccgg gtagaagtt cagccttac acgtcccga 
      atcctccgg ttggaggtc ggagacgtg gactccggc 
      gactacaaa ctggtggtt tccggaaag ggcggggtg 
      gatttttac tccgactat ccggtagag tttgtggaa 
      aaaagctac tcggtgttt atccaaacc gatcgagcg 
      gtttacaaa cccggatcc aaagtcttg ttccgggcg 
      gttgtgctc aactcgcag ttgaaaccg gcggcggaa 
      gtccggaat gaactactc cacattttt gtgacggac 
      gggcaaggg aatcggatc aaggagtgg aaaggaatc 
      caggcattg agaggggtc ttcaccggg gaagtgaaa 
      ctgagcgag agcccggtt ttgggaaac tggaatatt 
      tcggtcaaa atccacggg caaaccttc agcaaatca 
      atagaagtc gctgaatat attttacca aaattcatt 
      gtaaatatt caagcgccc aaacacatg actttcaag 
      gaaaacgtc cttgttgcc aatatccaa acccaatac 
      aattatggg aaaaaagtc aaaggagag gccactgtg 
      accgtttat cccacaatt ttctccggg gttattcaa 
      cctattttc cagaacccg atccggaaa gtaatcccg 
      atcgagggt tcggccact gtcacattc gacattgcc 
      aaagaactc aaactgact gacgaatac gaaagggtt 
      gtcatggtc gatgtaaca gttgaggaa gcttcaacg 
      ggccgccga caaaacaat tcggtcgaa gtccatttg 
      cataaatac aattataaa atggatcta attaaaaca 
      gcagattat ttcaaaccg gggctcaaa tacacagct 
      tatgtcaag gtttcaagt cacgaaggg actccccta 
      cgatcggaa aatagggaa gttacggtc agatacggc 
      tattcgcga gctgatgaa gtctatgtt actgagaaa 
      cacaggttg gataaaaac ggtgttgcg aaattggag 
      tattacact ccggtgaat gttacaaac accactgct 
      ctcaggatt gaggctcag taccaggac ctcaaggag 
      cgcatttcg ccaattcct gctgcggtt tcgtacagt 
      aacaccttc ctgcaagtg tcgctcgaa accgaaaga 
      ccgatcgta aacctcgat gttgaaatt ttagtcaat 
      tgcactgaa cgtttgaga tacataagc tacgtcctg 
      atgggacga ggcgacgtc ctgaatgcc aacaccttc 
      caagttgac aacatgcac gaatatcgg tttcatttc 
      acggcaaca catgccatg gtgccggtt gcccacctt 
      atagtgtct tacgtgcgt gacgatgga gaattggtg 
      ggagatgcc ctcgatatt gaagttgat gggcttttg 
      caaaactac atggagatt caagtcaat ccggtggaa 
      accgaaccc gagcttgat atcgacata gcgatcaga 
      gctcagccc aattcgtac gttgcgata atggcagtt 
      gaccagaac gcggtcaaa atgagacct gggtttgat 
      ttaacccac agtgaagta gcggaagaa ttgaaaaaa 
      tacgacccg gcccaacaa tcaccatat tccatgata 
      atgcacgac agcaagtac catttcttc tggaagccg 
      ggggctgcc aacccacac agtgccatt tacaattcg 
      ggggccgac ttattgacc aattctcac gtcgaccgc 
      caccaaccg actttggag gatatttac ttgcggccc 
      gttttctac ggcactagc accgtcaaa cccgaccgc 
      ggcttcggc ctgcctctc cacaccgtc accaggccg 
      cccctggca ggtccttac gccttcagt cgaatccct 
      aagcccgtt tggaacaaa ccgaaggtt tacttgact 
      gaggaaatc gccgatact tggcttttc accaacttc 
      tcgtcgggt tacgaaggc aaaacttca attcggcga 
      aaaatacca agctctctg aacacttgg gtcgtcact 
      ggcttttcc ctggacccc attcatggg ctgggactg 
      accacaact tccaagaaa gtcaaagtg tcgaaaagc 
      ttcgtcgtg acgcttgat ttgccgttt tcggtgcaa 
      cggcgagag attttagct gtgcctgtg gtcgtttac 
      aattatatg gacaaggat gtgaatgcg gaagtcaca 
      cttcacaac ccggaacaa aaattcgag tttgctgaa 
      gtttcaaat aatgttaac tcgaccaga aaagtggag 
      ttgtacagg cgcaaaaaa attaacatc aagcgaaac 
      agcggcacg tcagtctca ttcatgata cggccgttg 
      aaacaagac acaatcgag ataaaagtg accgcaaac 
      agtccgaaa aaccaggac gtggcaatt aagcatctc 
      caagtcacg actgaagga gagacggaa tattacaca 
      aaaaccgtc ctcattgat ttacgcaac aaccccaac 
      tacaaaaaa tcaatcaat ttcacaatc ccccaaaac 
      atggtgacc ggttcggaa aaaatcgaa gtttcagct 
      gttggtgat ctcctaggc ccgactatg gtccacctc 
      gaaaattta atcagactt ccaacagga tgcggtgaa 
      caaaatttg attcatctc atgccgaat ttaatcatt 
      ttgcaatat cttcgctac acgagacaa gttacccca 
      accattcaa aacgaagcg ttggatttg ctcgaaaag 
      ggttaccaa caacagttg agttataaa cgcaaggat 
      gggtcgttt agtgccttt ggaatgagg gatgagaag 
      agcagtgtt tgggttacg gcttatgtg gctcttact 
      ttaaggcaa gccaaaggc catatttac gttgacgag 
      aaaattatc gaaggttgt ctcgagtgg ttggcgaat 
      attcagggc cggaatggg agttttgtc gaagtgggg 
      tcagttatt tacaaggag attcagagt cgggaaggc 
      aatagttta gccttgacg gcttttacc ctcttggca 
      tttatcgaa aatcagaaa tatgcgtcc acttatagc 
      aatacaatt aacaaagga ttggattac attgcacgt 
      tacatttct gaacaagaa tcaatccat acgatagct 
      ttgtgtagc tacactctt caattagcc cgtcacccg 
      tcgaaacaa agcgctttc aacttgctt gacctccgt 
      tcaaagagc cgaggcaac ttgaaatgg tggtctaag 
      gacgtccca agtaacgag attaaaaat ccctggaac 
      aaattaccg cgttccatc gatatcgag acttcagcc 
      tacggtctt ttaaccttc cttgaggcc aattacttc 
      gaagacgca atccctgtc ctgaactgg ctactagac 
      cagcagaac agtctcgga ggtttcacc tcatcgcag 
      gacactttc gtgggcctt tgggccatt tataaacta 
      gtcctgaag ttggcaact aacgtaaat atgcaagtt 
      gagttcact tacggcaaa gaccagcgc cacaatttc 
      aacgttaat aaaaacaac gcaatgatt gtgcaaaaa 
      ctccaacta ccgaaagac atacgtgaa gtaaacgtg 
      acggctcag ggcaaaggc ttggccgtt tttagggtc 
      tcgtatgag tataatatg aacgtaacc gggccttgg 
      cccatgttt acgctggac ccacaagtg gacaaaaac 
      tcgaataag gaccactta caagtgtcg atttgcaca 
      ggatttgtg agtcgcaat ttgagtgaa acccctgaa 
      agcaacatg gcagtgatg gaagtgaac ctcccgtcg 
      ggtttcacc gccgatatc gactccttg ccaagcctg 
      gaagtgtcc caaaatgtg caaaaggtc gaaacgagc 
      aacggcctg actagagtc accctctac tttaacaac 
      gtgtcaagt gtaaacgaa tactgcccg acagtgtcc 
      gcttttagg acacataaa gtcgcaaac caaaaaccc 
      gtctcggtt attattttc gattattac gacagttcg 
      agacgtgcc aggcaattt taccgaagc cgaacgtca 
      actctgtgt gatatatgc gaggatgag gactgtggt 
      gatatttgc acctcaaag gcgaaccca caagtcggg 
      gaggacagt caggaaggc aaaggcagc tcggctgct 
      ctgcgttcg agttggttg ggtttccta gttgttatg 
      tttgccatt gtgcaattg tgattggtg ccggcggta 
      cttattgac gaaaccttt aacaaaata attcgctaa 
      tacgttcct cagtttggt agaaaaata gaattgttc 
      ttggtacaa ttatagtta tgtgttacc aactttacc 
      acgcaagtg gtgaaaagt gcgtcataa cattttact 
      aacactact agtcaccgg tctcagtaa ccccttagt 
      tgactgaat taagggtca agtgataaa ca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY