Status
Please wait ...
Gene information
General information
TC030587 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7883773..7889165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030587
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030587 RA
      MSYIRLGLH KWSNVNKQF LVALLSGVV VGFVTAYVI 
      LSPAILLVA LGDPHTSQE MESASGPSI DPGVHRHDE 
      EFHQMENST VADLMFKKV RILCWIMTG PSNHEKRAR 
      HVKATWGKR CNKLIFMSS QKDDSLPAV ALPVGEGRN 
      NLWGKTKEA FKYVYHNHM HEADWFLKA DDDTYVILE 
      NLRYMLLPY RSTDSIYFG CKFKPYVKQ GYMSGGAGY 
      VLSKEAVRK FVELGLNST KCSKSNSGA EDVEMGKCL 
      EAVEVKAGD SRDSVGRGR FFPFVPEHH LIPGHVKKS 
      FWYWQYIYY ESKEGMDCC SDNAVSFHY VTPNQMYVL 
      EYLIYHLRP YGISYQVEM PPELVEEHE KNKMTSL
    CDS sequence
    • >TC030587 RA
      atgagttac ataaggtta gggctacat aaatggtcg 
      aacgtaaac aagcaattt ctggtggcc ctcttgtcg 
      ggtgtcgtg gtggggttt gtgaccgcc tatgtgatt 
      ctctcgccg gcgattctg ctggtggct ttgggggat 
      ccccacacg agtcaggag atggagtct gcgtcgggg 
      ccctcgatc gatccgggg gttcaccga cacgatgaa 
      gagttccac cagatggag aatagtact gttgccgat 
      ttgatgttc aagaaagtg aggatcttg tgctggatt 
      atgacgggg cctagcaac cacgagaag agggcaaga 
      catgtcaag gccacgtgg ggcaagagg tgcaacaag 
      ttgatattc atgagttcg caaaaagat gactcgctt 
      cccgccgtg gccctgccc gtgggcgag ggccgcaac 
      aacctgtgg ggcaagacc aaggaggcg ttcaaatac 
      gtctaccac aaccacatg cacgaggct gattggttc 
      ctcaaagcc gacgacgac acctacgtg atattggag 
      aatttgcgt tacatgctc ctcccatac cgctccacc 
      gactccatc tacttcggt tgcaaattc aaaccgtac 
      gtcaaacag ggttacatg agcggtggc gctggttac 
      gtcctaagt aaggaggcg gtgcgaaaa ttcgtcgaa 
      ctcggcctg aactccacc aagtgttcc aagtccaac 
      agcggtgct gaagacgtc gaaatgggg aagtgtctc 
      gaagcggtg gaggtcaaa gccggggat tcacgtgat 
      tcggtcggg agaggtcgg ttttttccg ttcgtgccc 
      gaacatcac ctgattccg gggcacgtg aagaagagc 
      ttctggtat tggcagtat atttactac gagagtaaa 
      gaggggatg gactgttgt tcggataat gccgtgtcg 
      tttcattac gtcactccg aatcaaatg tacgtcttg 
      gagtacttg atttatcat ttaaggccg tatggaatc 
      tcgtatcag gtggaaatg ccgccggaa ttagtcgaa 
      gagcacgag aaaaataaa atgacttcc ttgtag
    mRNA sequence
    • >TC030587 RA
      tgagtaaaa agtaaacac agaagtggg ttggtgccc 
      ctgcgcagt gtcaaaatg gtgggacgc gaagttgga 
      tgtgtttat gatttcttt tgtgataaa caataattt 
      gtattaaat ccacggatt aaatcgaat taaattaac 
      tcaaaatgc aaaacaaca tgttcctga cgtgattcc 
      tacaacggt ctcattatg agttacata aggttaggg 
      ctacataaa tggtcgaac gtaaacaag caatttctg 
      gtggccctc ttgtcgggt gtcgtggtg gggtttgtg 
      accgcctat gtgattctc tcgccggcg attctgctg 
      gtggctttg ggggatccc cacacgagt caggagatg 
      gagtctgcg tcggggccc tcgatcgat ccgggggtt 
      caccgacac gatgaagag ttccaccag atggagaat 
      agtactgtt gccgatttg atgttcaag aaagtgagg 
      atcttgtgc tggattatg acggggcct agcaaccac 
      gagaagagg gcaagacat gtcaaggcc acgtggggc 
      aagaggtgc aacaagttg atattcatg agttcgcaa 
      aaagatgac tcgcttccc gccgtggcc ctgcccgtg 
      ggcgagggc cgcaacaac ctgtggggc aagaccaag 
      gaggcgttc aaatacgtc taccacaac cacatgcac 
      gaggctgat tggttcctc aaagccgac gacgacacc 
      tacgtgata ttggagaat ttgcgttac atgctcctc 
      ccataccgc tccaccgac tccatctac ttcggttgc 
      aaattcaaa ccgtacgtc aaacagggt tacatgagc 
      ggtggcgct ggttacgtc ctaagtaag gaggcggtg 
      cgaaaattc gtcgaactc ggcctgaac tccaccaag 
      tgttccaag tccaacagc ggtgctgaa gacgtcgaa 
      atggggaag tgtctcgaa gcggtggag gtcaaagcc 
      ggggattca cgtgattcg gtcgggaga ggtcggttt 
      tttccgttc gtgcccgaa catcacctg attccgggg 
      cacgtgaag aagagcttc tggtattgg cagtatatt 
      tactacgag agtaaagag gggatggac tgttgttcg 
      gataatgcc gtgtcgttt cattacgtc actccgaat 
      caaatgtac gtcttggag tacttgatt tatcattta 
      aggccgtat ggaatctcg tatcaggtg gaaatgccg 
      ccggaatta gtcgaagag cacgagaaa aataaaatg 
      acttccttg tagtttgct caaatttga ggaatcact 
      agtcgatat attaccgat gtgagatta tttttaggg 
      tttttgtaa cgattgcca tattttttc gattcgcgg 
      tttcagcta cattctgta ctcatttga aacaatgat 
      tctcttttt gttttttcc ccgtgttca aataatata 
      ctcaaacta gatatttga ttatgaaaa atttacacc 
      agagggcgt attttttgg aatcgcgaa ttatttctt 
      ctatgacgt aaggaacac acctatggt aggtataaa 
      gtacagttg gttttccta aaaatgaca cttgtgaga 
      aatgagaca ttctttttg ggtctgact gtaccacat 
      agatgactg ttgaaagtg acaaccaaa tgcaatctc 
      ttgtatact aattaggga tttttttat ttgaagttt 
      ttattttta aaaacaagt cctatacca aagatatgc 
      tattttaaa gtaagtttt attcgatga tgattttat 
      atctttcag ctttttgta gctgttttg ttgacgtaa 
      tatttttaa tttgtttat attacttta tcaaccatt 
      tacgtaaac tatatcacg ttaaagcgc caataaatg 
      atgcgattt ttgctcaat atgctcaat aaaatatca 
      gttttaaca taattttat ttgtgccgc gaaaacaat 
      catttacgc tctagatac aaaacataa ttattacga 
      gtgtttccg taaattaag tgttgtaaa tcttatttt 
      gttcctgga actgaaaaa cttaattca ttcatttat 
      tagacttag agtcggttt atagaagcg tcattaatt 
      taattggca attaaatgc gtgattaac tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY