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Gene information
General information
TC014265 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8132069..8135187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014265
    iBeetle screen iB_10959

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014265 RA
      MRALVVSIW VFINLVFFG MCGYMYLLW SQYWMFMER 
      QTGTTTSTY DQQIYYYNR GFLPNDNKT HNETKLRIK 
      HSLKDNVTV TLIKNSKPR FPNLQGREF ELDTKKYIC 
      LKNDSFKNC SSKSLEFKE KILKELRRV LEDEGNVLK 
      IGAENPYNV QYEGTKGNF YDKTPKEIM CELEKIQVK 
      TLKRGDVAG GPHNLGDFL PKRGLFENR NFNSCAIVA 
      SSGALKDSN LGKKIDAHD LVLRFNHAP TKGFEPDVG 
      RKTTIRVLN SQVVTRKEF NFLESDIYK NVTIVAWDP 
      SNYKASLDD WLEKPEFNL FPTYVEYKK RNDKARFFL 
      INPQSLWEL WDFLQDNSP SRLRRNPLS SGFLGLGIL 
      LPLCNFIDV FEYVPSTRV TKRCHYYDP EDNPACTFG 
      VWHPLAAEK LLTYYINTI DDRTVFQDG YVRILGFKN 
      FKC
    CDS sequence
    • >TC014265 RA
      atgagagct ctagtggta tccatatgg gtttttatc 
      aatttggtg tttttcggt atgtgtgga tacatgtat 
      ctgctgtgg tcgcagtac tggatgttc atggagcgg 
      caaaccgga acaacaact tccacctac gaccagcaa 
      atttactac tacaaccgg gggtttcta ccaaatgat 
      aacaaaacc cataacgaa accaagctt agaatcaag 
      cacagcctt aaagataac gtcacagta actttaatt 
      aaaaacagt aagcccaga ttccccaat ttgcaaggc 
      agggagttc gaattagac acgaaaaaa tatatttgt 
      ttaaaaaat gatagtttt aaaaactgt tcgagcaaa 
      tcgttagag tttaaggaa aaaatcctg aaggagttg 
      cgccgcgtc ctggaggac gagggcaac gttcttaaa 
      ataggcgca gagaacccc tacaacgtg caatatgaa 
      ggcaccaaa ggcaacttc tatgacaaa acgcccaag 
      gaaataatg tgcgaactg gagaaaatt caagtaaaa 
      acgttgaaa cgcggcgac gtggccggg ggtccccac 
      aacctgggc gatttcctc cccaaaagg gggctgttc 
      gagaaccgc aacttcaac tcttgtgcg attgttgcg 
      agctccggc gcgctcaaa gactcaaat ttgggcaag 
      aaaatcgac gctcatgat ttggttttg cgcttcaat 
      cacgcgccg accaaaggc ttcgaacca gatgtaggc 
      agaaagaca acaatccgc gtgttgaac tcgcaagtt 
      gttaccagg aaagaattc aatttcctc gaatccgat 
      atttataaa aatgtaacg attgtcgcg tgggatccg 
      agtaattat aaagcgtct cttgatgac tggcttgaa 
      aaaccggaa tttaatttg ttcccgacc tatgttgaa 
      tacaaaaag cggaatgat aaagcccga tttttccta 
      ataaatccg caaagtctg tgggagttg tgggatttt 
      ctccaggat aactcaccg agcagactt aggagaaat 
      ccactatcg tcggggttt ttgggtttg ggtattttg 
      cttcctctg tgcaatttc atcgatgtt tttgagtat 
      gtcccttca accagggtc actaaacgc tgccactat 
      tacgatcct gaagataat ccagcttgt actttcgga 
      gtttggcat ccattagca gccgaaaaa cttcttact 
      tattacatt aatacgatc gatgatcgg acagtgttt 
      caagatggt tatgttcga atcttgggt ttcaaaaat 
      tttaaatgt tga
    mRNA sequence
    • >TC014265 RA
      aacgacaaa aaatcaacc agaactgca aaatcatag 
      cgccaaaat gagagctct agtggtatc catatgggt 
      ttttatcaa tttggtgtt tttcggtat gtgtggata 
      catgtatct gctgtggtc gcagtactg gatgttcat 
      ggagcggca aaccggaac aacaacttc cacctacga 
      ccagcaaat ttactacta caaccgggg gtttctacc 
      aaatgataa caaaaccca taacgaaac caagcttag 
      aatcaagca cagccttaa agataacgt cacagtaac 
      tttaattaa aaacagtaa gcccagatt ccccaattt 
      gcaaggcag ggagttcga attagacac gaaaaaata 
      tatttgttt aaaaaatga tagttttaa aaactgttc 
      gagcaaatc gttagagtt taaggaaaa aatcctgaa 
      ggagttgcg ccgcgtcct ggaggacga gggcaacgt 
      tcttaaaat aggcgcaga gaaccccta caacgtgca 
      atatgaagg caccaaagg caacttcta tgacaaaac 
      gcccaagga aataatgtg cgaactgga gaaaattca 
      agtaaaaac gttgaaacg cggcgacgt ggccggggg 
      tccccacaa cctgggcga tttcctccc caaaagggg 
      gctgttcga gaaccgcaa cttcaactc ttgtgcgat 
      tgttgcgag ctccggcgc gctcaaaga ctcaaattt 
      gggcaagaa aatcgacgc tcatgattt ggttttgcg 
      cttcaatca cgcgccgac caaaggctt cgaaccaga 
      tgtaggcag aaagacaac aatccgcgt gttgaactc 
      gcaagttgt taccaggaa agaattcaa tttcctcga 
      atccgatat ttataaaaa tgtaacgat tgtcgcgtg 
      ggatccgag taattataa agcgtctct tgatgactg 
      gcttgaaaa accggaatt taatttgtt cccgaccta 
      tgttgaata caaaaagcg gaatgataa agcccgatt 
      tttcctaat aaatccgca aagtctgtg ggagttgtg 
      ggattttct ccaggataa ctcaccgag cagacttag 
      gagaaatcc actatcgtc ggggttttt gggtttggg 
      tattttgct tcctctgtg caatttcat cgatgtttt 
      tgagtatgt cccttcaac cagggtcac taaacgctg 
      ccactatta cgatcctga agataatcc agcttgtac 
      tttcggagt ttggcatcc attagcagc cgaaaaact 
      tcttactta ttacattaa tacgatcga tgatcggac 
      agtgtttca agatggtta tgttcgaat cttgggttt 
      caaaaattt taaatgttg agttttgag aaattttgg 
      agtgaattt ttagacgtc aattaattc gtaagatga 
      tgagtcata gattttgtc ggttgattt ccgcttttg 
      catattatt gtgtgagtg taagcggtt ttgcggtga 
      attaagaac acccatcgt ctgaacact gtttccgga 
      atttcaaac ttttgttct tccctattt atttcatgc 
      ttaaatttg attcggact aattttagt ctgaaaaaa 
      tcttgtgat attctaaaa atagagaca actttaaat 
      ctgaaataa aattgtttc agtagtgtt taattaaaa 
      taaatacaa actgtacta atttgttta atgtagctc 
      aaattatga aaataaaat atggcatga aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY