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Gene information
General information
TC015234 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9370115..9374612
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015234
    iBeetle screen iB_10958

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015234 RA
      MRIALWTMI ITDVLLTTT LPHDQWTND DPHNLTKRK 
      ESDYFDKND ANKENESDD NGKSESYNK IDKRELDDA 
      VDYGIRAMQ ELIEVKEPK WYKMGLYLD AKEPAHYVA 
      NFGAPIKKA IELSNFGYA SLEATKRLA ETYPENVSR 
      QAAGSFDTR LREDVCPLR GTPRCPAAS RRYRTADGT 
      CNNFNKPWR GSSLLPMQR FLQPVYEDG IQEPRRSIF 
      GHKLPSARE ISTRIHRDK NFEVPTVSL MFMQWGQLI 
      DHDLVSTVK SRSFNGTVP RCCRRGGQA LLPPELTHP 
      SCLPIEVSP DDWFLSSFG LRCIEFIRS APSTRIDCD 
      LGWREQINQ VTSYLDASP IYGSDIETS DSMRVFRKG 
      KLHYGRPQG REPLQPPDP PGGELCRSG AVTTDCFQG 
      GDGRFSEQP GLTALHTVF VRYHNRLAT VLGQVNRHW 
      SDEKVYQET RRIVVAIMQ HVTYREFLP IVLGPEVID 
      LFELKLERK GYYSGYDDR VNPEVANAF GSAAFRFGH 
      SMVQNSFVR FDTKHRPLF NNVTLHEEQ ENVENIWSL 
      GSLDRLLLG FCNQPSQRR DEFICDELT NHLFQSRGF 
      PFGMDLAAI NVQRGRDHG LPPYTSWRE PCGLSPIKS 
      WKDLEKIMN PDTVHRFES LYEDINDID LFSGGLAEK 
      PVRGGIIGP TFACIIAQQ FLNLRKGDR FWYENGGFE 
      SSFTPAQLQ QIRHVTLAH VLCQTLTEI ETIQPFVFL 
      TVDGFRNSR LSCDDPVID NFDLSPWVE EDLNEVRRE 
      GRKRRKLKR TTTKKPKVA TPVKVKVYN TTTHTVKVQ 
      TKQTYTDSY GNVPDNIPQ RPTFDSYND RRTTRPDVT 
      YLFGVVSES TTPIPTPKP FQVNIKIQY YPPTTPQPT 
      RRTRRPYDS TTKQNFYSD DYSVYRPQN YYQSKYEDQ 
      PFTSSKRPY IYSNNYVYS KRRPQDDAF DRLYSDVYS 
      TPNFEDQDD LPRPFSHVE KFSTNYEHD KLDFYTPKK 
      QSSGTKFVK ISSVKGQEY LDPSGATVQ LHVSQREGD 
      LELDEGGTT DDVRLVDLE VLPGDSKWL VYNATEEEP 
      DVMVLPDVN LNVSCSSEL PRPFLRRNL TVPET
    CDS sequence
    • >TC015234 RA
      atgagaatt gcgctatgg acgatgata atcacggac 
      gttttactg acgacgaca ttaccacac gaccaatgg 
      actaacgac gacccacat aacttgacc aaaaggaaa 
      gaaagcgac tatttcgac aagaacgac gcaaataag 
      gaaaacgaa agcgatgat aatggcaag agtgaaagc 
      tacaacaaa attgataag cgggagctg gacgatgcg 
      gtcgactac gggatcagg gccatgcag gaacttatc 
      gaggtcaaa gaaccgaaa tggtacaaa atgggcttg 
      tacttggac gcgaaagag ccggctcac tacgttgcg 
      aacttcggc gcccccatc aaaaaagcc atcgagctg 
      tcgaacttc ggttacgcc tccctggaa gccaccaaa 
      cgacttgca gaaacttat ccggaaaat gtgagtcga 
      caagccgcc ggcagcttc gacacgaga ctccgggag 
      gatgtgtgt ccgctaagg ggcaccccc aggtgtccc 
      gcagcctcc cgacggtac cgaacagcc gatggtacc 
      tgcaacaac ttcaacaag ccatggagg ggctcctcg 
      ctcctgccc atgcagagg ttcctgcaa ccggtctac 
      gaggacggc atccaagag cctcgccga tcaatcttc 
      gggcacaag ctgccgtca gcgagggaa atcagcacc 
      agaatccac cgcgacaag aattttgaa gtaccaacg 
      gtcagcctc atgttcatg caatggggg caactgatc 
      gaccacgac ctggtctcc actgtcaag tcgcgcagc 
      ttcaacggc acggtcccc aggtgctgc cgccgcggc 
      ggacaggcc cttcttccg ccggagctc acgcatcca 
      agctgcctc ccgatcgaa gtctccccc gacgactgg 
      ttcctctcc tccttcggc ctccgctgc atcgaattc 
      atccgaagt gccccttcg acccgcatc gactgtgac 
      ctaggttgg cgtgagcaa atcaaccaa gtcacttca 
      tatctcgat gcgtcccca atttacggc agcgacatc 
      gaaacatcc gactcaatg cgagttttc cgcaagggg 
      aagctccat tacgggcgt ccccaaggg cgcgaaccc 
      ctccaaccg cctgatccc ccaggtggg gaactttgc 
      cgatcagga gccgtcact acagactgc ttccaaggg 
      ggtgacggc cgttttagc gaacagcca ggtcttacg 
      gcactccac actgtattc gtgaggtac cataaccgc 
      ctggcaacg gttttgggg caagttaac cggcactgg 
      agcgacgag aaggtgtac caagagacg agaagaatc 
      gtcgttgcg attatgcaa catgtgacc tatcgggag 
      ttcctcccg attgttcta ggaccggaa gttatcgat 
      ttgtttgag ctgaagttg gagcggaaa gggtactac 
      tccggttat gacgatcgg gtgaatccg gaggttgca 
      aatgccttt gggagtgcg gctttccgg tttgggcac 
      agtatggta caaaattct ttcgtcagg tttgatacc 
      aagcaccgg ccactcttt aacaatgtg acccttcat 
      gaggagcaa gaaaacgtg gagaatatc tggagtttg 
      gggtctttg gaccggctt ttgctgggc ttttgcaac 
      caaccgtcg caaagacgc gacgaattc atttgtgac 
      gaactcact aaccatttg ttccaatcc cgggggttc 
      cccttcggg atggacctc gctgctatt aatgttcaa 
      agggggcgc gaccatggc ctccccccg tacacctcc 
      tggcgcgag ccttgcggt ttaagccct atcaagtca 
      tggaaagac ttggaaaaa atcatgaac ccggacaca 
      gtccaccga ttcgaaagt ctctacgag gatatcaac 
      gatatcgac ctattttcg gggggtttg gccgagaaa 
      cccgtgcgt gggggcatc ataggcccc acttttgcc 
      tgtatcata gcccaacag ttcctaaat ttgcggaaa 
      ggtgaccgg ttttggtac gaaaacggc ggatttgag 
      tcgagcttc actcctgcg caactgcaa caaatccgc 
      cacgtgacc cttgcgcat gtcctttgc cagactttg 
      acggagatt gaaacgatc caacctttt gtttttctg 
      actgttgat ggctttcgc aactctcga ttgtcctgc 
      gacgatcct gtcattgac aacttcgat ctttcgccg 
      tgggttgaa gaggacttg aatgaagtt cggagagag 
      gggaggaag agacggaag ttgaaacgt actactact 
      aaaaaaccc aaagttgcg actcccgtc aaagttaag 
      gtttacaac acgacgact cacacggtt aaagtacaa 
      acgaaacag acttacact gacagttac ggcaacgta 
      ccagataac ataccacaa agaccgact tttgacagt 
      tacaatgac cgacgcacg actagacct gacgtgact 
      tacctattt ggggttgta tccgagtcc acaacccct 
      attccaact cccaaaccg ttccaagtc aatatcaaa 
      attcagtac taccctccg acgactccc caaccgacc 
      agacgaacg cgacgcccg tacgactcg acgaccaaa 
      cagaatttt tacagtgac gactactcg gtctataga 
      ccgcaaaac tactaccaa agcaaatac gaagaccag 
      cctttcacg tcctcaaaa cgcccctac atttactca 
      aacaactac gtttattca aaacggcga ccgcaagac 
      gatgctttc gacagactt tacagcgac gtctactcg 
      actcctaat tttgaagac caagacgat ttgccacgt 
      cctttcagc cacgtggaa aaattttcg accaactac 
      gaacacgac aaactggac ttctacacc cccaaaaag 
      caatccagt ggtaccaaa ttcgtgaaa atctcaagc 
      gtgaagggc caggagtat ctagacccg agcggagcc 
      accgttcaa ttacacgtt tcgcaacgt gaaggcgac 
      ttagaactt gacgaaggt ggtaccact gacgatgtg 
      agactggta gacctggag gtactaccc ggagactca 
      aaatggcta gtttacaac gccacggaa gaggaacca 
      gacgtgatg gtacttccc gatgtcaat ctcaacgtt 
      tcctgctcg agcgaactt cctagacct ttccttaga 
      cgcaacttg acagtacct gagacttga
    mRNA sequence
    • >TC015234 RA
      acgactctc tcgattctc gagatctcc gttcagttt 
      ttaacttgc catccgtgc gtgcgaaaa tgagaattg 
      cgctatgga cgatgataa tcacggacg ttttactga 
      cgacgacat taccacacg accaatgga ctaacgacg 
      acccacata acttgacca aaaggaaag aaagcgact 
      atttcgaca agaacgacg caaataagg aaaacgaaa 
      gcgatgata atggcaaga gtgaaagct acaacaaaa 
      ttgataagc gggagctgg acgatgcgg tcgactacg 
      ggatcaggg ccatgcagg aacttatcg aggtcaaag 
      aaccgaaat ggtacaaaa tgggcttgt acttggacg 
      cgaaagagc cggctcact acgttgcga acttcggcg 
      cccccatca aaaaagcca tcgagctgt cgaacttcg 
      gttacgcct ccctggaag ccaccaaac gacttgcag 
      aaacttatc cggaaaatg tgagtcgac aagccgccg 
      gcagcttcg acacgagac tccgggagg atgtgtgtc 
      cgctaaggg gcaccccca ggtgtcccg cagcctccc 
      gacggtacc gaacagccg atggtacct gcaacaact 
      tcaacaagc catggaggg gctcctcgc tcctgccca 
      tgcagaggt tcctgcaac cggtctacg aggacggca 
      tccaagagc ctcgccgat caatcttcg ggcacaagc 
      tgccgtcag cgagggaaa tcagcacca gaatccacc 
      gcgacaaga attttgaag taccaacgg tcagcctca 
      tgttcatgc aatgggggc aactgatcg accacgacc 
      tggtctcca ctgtcaagt cgcgcagct tcaacggca 
      cggtcccca ggtgctgcc gccgcggcg gacaggccc 
      ttcttccgc cggagctca cgcatccaa gctgcctcc 
      cgatcgaag tctcccccg acgactggt tcctctcct 
      ccttcggcc tccgctgca tcgaattca tccgaagtg 
      ccccttcga cccgcatcg actgtgacc taggttggc 
      gtgagcaaa tcaaccaag tcacttcat atctcgatg 
      cgtccccaa tttacggca gcgacatcg aaacatccg 
      actcaatgc gagttttcc gcaagggga agctccatt 
      acgggcgtc cccaagggc gcgaacccc tccaaccgc 
      ctgatcccc caggtgggg aactttgcc gatcaggag 
      ccgtcacta cagactgct tccaagggg gtgacggcc 
      gttttagcg aacagccag gtcttacgg cactccaca 
      ctgtattcg tgaggtacc ataaccgcc tggcaacgg 
      ttttggggc aagttaacc ggcactgga gcgacgaga 
      aggtgtacc aagagacga gaagaatcg tcgttgcga 
      ttatgcaac atgtgacct atcgggagt tcctcccga 
      ttgttctag gaccggaag ttatcgatt tgtttgagc 
      tgaagttgg agcggaaag ggtactact ccggttatg 
      acgatcggg tgaatccgg aggttgcaa atgcctttg 
      ggagtgcgg ctttccggt ttgggcaca gtatggtac 
      aaaattctt tcgtcaggt ttgatacca agcaccggc 
      cactcttta acaatgtga cccttcatg aggagcaag 
      aaaacgtgg agaatatct ggagtttgg ggtctttgg 
      accggcttt tgctgggct tttgcaacc aaccgtcgc 
      aaagacgcg acgaattca tttgtgacg aactcacta 
      accatttgt tccaatccc gggggttcc ccttcggga 
      tggacctcg ctgctatta atgttcaaa gggggcgcg 
      accatggcc tccccccgt acacctcct ggcgcgagc 
      cttgcggtt taagcccta tcaagtcat ggaaagact 
      tggaaaaaa tcatgaacc cggacacag tccaccgat 
      tcgaaagtc tctacgagg atatcaacg atatcgacc 
      tattttcgg ggggtttgg ccgagaaac ccgtgcgtg 
      ggggcatca taggcccca cttttgcct gtatcatag 
      cccaacagt tcctaaatt tgcggaaag gtgaccggt 
      tttggtacg aaaacggcg gatttgagt cgagcttca 
      ctcctgcgc aactgcaac aaatccgcc acgtgaccc 
      ttgcgcatg tcctttgcc agactttga cggagattg 
      aaacgatcc aaccttttg tttttctga ctgttgatg 
      gctttcgca actctcgat tgtcctgcg acgatcctg 
      tcattgaca acttcgatc tttcgccgt gggttgaag 
      aggacttga atgaagttc ggagagagg ggaggaaga 
      gacggaagt tgaaacgta ctactacta aaaaaccca 
      aagttgcga ctcccgtca aagttaagg tttacaaca 
      cgacgactc acacggtta aagtacaaa cgaaacaga 
      cttacactg acagttacg gcaacgtac cagataaca 
      taccacaaa gaccgactt ttgacagtt acaatgacc 
      gacgcacga ctagacctg acgtgactt acctatttg 
      gggttgtat ccgagtcca caaccccta ttccaactc 
      ccaaaccgt tccaagtca atatcaaaa ttcagtact 
      accctccga cgactcccc aaccgacca gacgaacgc 
      gacgcccgt acgactcga cgaccaaac agaattttt 
      acagtgacg actactcgg tctatagac cgcaaaact 
      actaccaaa gcaaatacg aagaccagc ctttcacgt 
      cctcaaaac gcccctaca tttactcaa acaactacg 
      tttattcaa aacggcgac cgcaagacg atgctttcg 
      acagacttt acagcgacg tctactcga ctcctaatt 
      ttgaagacc aagacgatt tgccacgtc ctttcagcc 
      acgtggaaa aattttcga ccaactacg aacacgaca 
      aactggact tctacaccc ccaaaaagc aatccagtg 
      gtaccaaat tcgtgaaaa tctcaagcg tgaagggcc 
      aggagtatc tagacccga gcggagcca ccgttcaat 
      tacacgttt cgcaacgtg aaggcgact tagaacttg 
      acgaaggtg gtaccactg acgatgtga gactggtag 
      acctggagg tactacccg gagactcaa aatggctag 
      tttacaacg ccacggaag aggaaccag acgtgatgg 
      tacttcccg atgtcaatc tcaacgttt cctgctcga 
      gcgaacttc ctagacctt tccttagac gcaacttga 
      cagtacctg agacttgac ctgacctga cttagtttt 
      aatttattc taatgtttc gaaaatttt cgactggat 
      tcttaaaag taccggcta aaatactgc caccatggg 
      tgatatgtc ttatttatt aatttttgt atttattgt 
      tttggtgta ataaagcta ttttattaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY