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Gene information
General information
TC004951 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4984238..4985507
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004951
    iBeetle screen iB_10957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004951 RA
      MSRRLNNGY CIPLIGLGI HKLVHAEEI RQVVKLAID 
      AGYRHFDCA WIYGTEAAV AKAIHEKIK EGVITREDV 
      YITTKVWNN YHQRKDVVE KLRESLQEM KLCYVDLYL 
      IHWPLGFKV SANSLPLNE GHSAYSDVD YVETWRGME 
      DCVYLGYTR SIGLANFNS EQINRILKN ANIIPTVNH 
      IEVHPRLNQ KKMIQFCAQ RNILVTGYC PLGINEMSG 
      VPNFPEPTI CDEKIDEIA RKHHKTAAQ VVLNYLISL 
      GICVVTRCS NQFRIREHI DIFDFQLDP QDVEYLDSC 
      NRNQRICAF SAFVDHKYY PFRLEF
    CDS sequence
    • >TC004951 RA
      atgtctcga agactcaac aacggctac tgcatccct 
      ttgattggg ctaggaatc cacaaactt gttcacgct 
      gaggaaatc cgccaagtg gtcaaactg gcaatcgac 
      gccggttat cgccacttt gactgtgcc tggatttac 
      ggaactgaa gccgcagtg gccaaagca atccacgaa 
      aaaatcaaa gaaggtgta atcacacgt gaagacgtc 
      tacataacc accaaagtt tggaataac taccaccag 
      cgcaaggat gtggtggag aaactgcgt gagtcgttg 
      caggaaatg aaactttgt tacgtcgat ctgtatctg 
      attcattgg ccgcttggg tttaaggtc agtgcgaat 
      tctttgccc ctgaatgaa gggcacagt gcgtacagt 
      gacgtcgat tacgtggaa acctggcgc ggaatggaa 
      gattgtgtc tacctgggt tacacaaga agcatcggt 
      ttggcaaac tttaacagt gaacaaatt aacagaatc 
      ctcaaaaac gccaatatc ataccaaca gtgaaccac 
      atcgaagtc cacccgcgt ctcaaccag aagaaaatg 
      atccagttt tgcgcccaa cgcaacatt ttagttaca 
      ggatactgt cctttaggg attaacgaa atgagtggc 
      gtcccgaat ttcccagaa cctacgatt tgtgacgag 
      aaaattgac gaaattgca cgcaagcac cacaaaacc 
      gctgctcaa gtcgttcta aattatttg atttcgtta 
      ggtatttgt gtggtcacg cgttgcagt aaccagttc 
      agaattagg gaacatatt gacattttt gactttcaa 
      ctagacccc caagatgtg gaatatttg gattcgtgc 
      aaccggaat cagaggatt tgcgcattt tcggctttc 
      gtcgaccac aaatattac ccgtttcgc ttggagttt 
      taa
    mRNA sequence
    • >TC004951 RA
      taatattta tcaaattca caattgatt aaaagtgat 
      aaaaattat cacaagata attttttaa acatcatcg 
      accgtgaaa atgttaaaa catgtctcg aagactcaa 
      caacggcta ctgcatccc tttgattgg gctaggaat 
      ccacaaact tgttcacgc tgaggaaat ccgccaagt 
      ggtcaaact ggcaatcga cgccggtta tcgccactt 
      tgactgtgc ctggattta cggaactga agccgcagt 
      ggccaaagc aatccacga aaaaatcaa agaaggtgt 
      aatcacacg tgaagacgt ctacataac caccaaagt 
      ttggaataa ctaccacca gcgcaagga tgtggtgga 
      gaaactgcg tgagtcgtt gcaggaaat gaaactttg 
      ttacgtcga tctgtatct gattcattg gccgcttgg 
      gtttaaggt cagtgcgaa ttctttgcc cctgaatga 
      agggcacag tgcgtacag tgacgtcga ttacgtgga 
      aacctggcg cggaatgga agattgtgt ctacctggg 
      ttacacaag aagcatcgg tttggcaaa ctttaacag 
      tgaacaaat taacagaat cctcaaaaa cgccaatat 
      cataccaac agtgaacca catcgaagt ccacccgcg 
      tctcaacca gaagaaaat gatccagtt ttgcgccca 
      acgcaacat tttagttac aggatactg tcctttagg 
      gattaacga aatgagtgg cgtcccgaa tttcccaga 
      acctacgat ttgtgacga gaaaattga cgaaattgc 
      acgcaagca ccacaaaac cgctgctca agtcgttct 
      aaattattt gatttcgtt aggtatttg tgtggtcac 
      gcgttgcag taaccagtt cagaattag ggaacatat 
      tgacatttt tgactttca actagaccc ccaagatgt 
      ggaatattt ggattcgtg caaccggaa tcagaggat 
      ttgcgcatt ttcggcttt cgtcgacca caaatatta 
      cccgtttcg cttggagtt ttaaataaa ttatttatg 
      cagttttga ccatgactt ttctttgat aacaaatga 
      tacattgga tttattttt gaattaatt aaaataatt 
      tcgactat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY