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Gene information
General information
TC014863 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9524252..9543942
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014863
    iBeetle screen iB_10951

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014863 RA
      MSWESLNSH DFTRYGGVS HQLSATRLL SSPDGSRHP 
      LDGLEQIWP WNDSYSSLH RDTYTEVRT PRPPIFTWG 
      CFRACRARI YQSIAKRKV DQGVKLYNQ HKQQQAVRK 
      WKSALKAIR KREDKFVLL GYLCQAYMD WGKYRDALE 
      YAHRQLFIS EELDNPNMR AESYLSLAK AHQRLGGLE 
      KALAYARHS LYNECEQCT TAGHVHLTV GSVYLELAG 
      FNKALDSFQ HAHRIAQAV HDPALELQV YVGLSELFS 
      RLQDADKSA KYASKAYDL SRSLQLGDL NSRHHRAAL 
      LQMASALRK QGELGDAHD YCSEATRLA LMSGDQATY 
      ARSIRIMGD IYRKRTDIN KAFRQYETA MGSAAAMGD 
      RVIQMESMD GAARCLEAL RLQHKICNC RPLEFNTRL 
      LEVASSVGA KLLVRTVRL RLSRIYHAL GDEDHRLHH 
      ERVAFRLQQ DLDLMCGGC GSPYGLESD SLEALPCAH 
      ILHARCAYD LFRRSSKKK KRSCPECDR TSSRLYLNC 
      TEYNTSYSP VPLSVCSSD SHCTSHQAT SSV
    CDS sequence
    • >TC014863 RA
      atgtcgtgg gaatcgctc aactcgcac gacttcacc 
      aggtatggc ggggttagt caccagctt tcagccaca 
      cgtcttcta tcatctccc gacggctct cggcacccc 
      ctcgacggc ctcgagcag atctggcct tggaacgac 
      tcatactcg agccttcac cgtgacacc tacacggag 
      gttcgcacc ccaagaccc ccaattttc acctggggc 
      tgttttcga gcctgtcgg gcacgaatc taccaaagc 
      atcgccaaa agaaaggtc gatcaagga gtaaagctt 
      tataaccag cacaagcag caacaagcc gtcagaaag 
      tggaagtcg gctctgaaa gctatcaga aagcgggag 
      gacaaattt gtgctttta gggtatttg tgtcaggcg 
      tatatggac tgggggaag tacagggat gcgttagag 
      tacgcacac cgccaactt ttcataagt gaggagctg 
      gacaatcca aatatgagg gcagagtcc tacttgagt 
      ttagcaaag gcccaccaa agactaggt ggccttgaa 
      aaggccttg gcatacgcc cggcactcc ctgtacaac 
      gaatgcgag caatgcacg accgccggc catgtccac 
      ctcaccgtc ggctccgtc tacttggag ctggccggc 
      ttcaacaag gcccttgat agcttccag cacgcccat 
      cgcatcgcc caggccgtc cacgaccca gcactcgaa 
      ttgcaagtc tacgttggg ctttctgag cttttcagc 
      cgtttgcaa gacgccgat aagtcggca aaatacgct 
      tcaaaagcc tacgatctc tcgcgatct ctccaactg 
      ggagatttg aattcgcga catcatcga gctgcgctt 
      ctgcagatg gcgtcggcg ttgcggaag caaggcgag 
      ttgggagat gcacacgat tattgttcg gaggcgacg 
      aggttggcg ctgatgtcc ggggaccaa gcgacttat 
      gcaaggagt atacgaatc atgggggat atctacagg 
      aagaggact gatattaat aaagctttc agacagtac 
      gagacagcg atgggttcg gcggcggcg atgggcgac 
      cgcgtcatc cagatggag tcaatggat ggggcggca 
      agatgcttg gaagcacta agactccag cacaaaatt 
      tgcaactgc cgaccatta gaattcaat acaagactg 
      ttagaagta gctagttcc gtcggtgct aaattgttg 
      gtgcgcacg gtgcgcctg cgtctctcg cgcatctat 
      cacgcccta ggagacgaa gaccaccgt ctccaccac 
      gaaagggtc gccttccgt ctccagcag gacctggac 
      ctcatgtgc ggcggctgc ggctctcct tacggcctt 
      gaaagcgac tcacttgaa gctcttccc tgtgctcat 
      atcttacac gcgagatgt gcctacgat ttgttccga 
      aggagtagt aaaaagaag aaacggtcg tgtcctgaa 
      tgcgatcgg actagttct aggttatat ttgaattgt 
      acagagtac aacacttcg tacagtccg gtgccgttg 
      tctgtctgt tcttccgac agtcactgt acgtcgcat 
      caagccacc agttcggtt taa
    mRNA sequence
    • >TC014863 RA
      ctccgaaat aatgatctg gttgtgctt cagtcgggg 
      atgctggat ggtggtcga aggggtgtg ttttgacgt 
      cgtgtacat attgagaaa aaaactcga tttttacgt 
      ttttttcgt tcacaaaat cggcctatt tttgcaaaa 
      tgcgcttaa acaaattta gtggttgaa caggatgtc 
      gtgggaatc gctcaactc gcacgactt caccaggta 
      tggcggggt tagtcacca gctttcagc cacacgtct 
      tctatcatc tcccgacgg ctctcggca ccccctcga 
      cggcctcga gcagatctg gccttggaa cgactcata 
      ctcgagcct tcaccgtga cacctacac ggaggttcg 
      caccccaag acccccaat tttcacctg gggctgttt 
      tcgagcctg tcgggcacg aatctacca aagcatcgc 
      caaaagaaa ggtcgatca aggagtaaa gctttataa 
      ccagcacaa gcagcaaca agccgtcag aaagtggaa 
      gtcggctct gaaagctat cagaaagcg ggaggacaa 
      atttgtgct tttagggta tttgtgtca ggcgtatat 
      ggactgggg gaagtacag ggatgcgtt agagtacgc 
      acaccgcca acttttcat aagtgagga gctggacaa 
      tccaaatat gagggcaga gtcctactt gagtttagc 
      aaaggccca ccaaagact aggtggcct tgaaaaggc 
      cttggcata cgcccggca ctccctgta caacgaatg 
      cgagcaatg cacgaccgc cggccatgt ccacctcac 
      cgtcggctc cgtctactt ggagctggc cggcttcaa 
      caaggccct tgatagctt ccagcacgc ccatcgcat 
      cgcccaggc cgtccacga cccagcact cgaattgca 
      agtctacgt tgggctttc tgagctttt cagccgttt 
      gcaagacgc cgataagtc ggcaaaata cgcttcaaa 
      agcctacga tctctcgcg atctctcca actgggaga 
      tttgaattc gcgacatca tcgagctgc gcttctgca 
      gatggcgtc ggcgttgcg gaagcaagg cgagttggg 
      agatgcaca cgattattg ttcggaggc gacgaggtt 
      ggcgctgat gtccgggga ccaagcgac ttatgcaag 
      gagtatacg aatcatggg ggatatcta caggaagag 
      gactgatat taataaagc tttcagaca gtacgagac 
      agcgatggg ttcggcggc ggcgatggg cgaccgcgt 
      catccagat ggagtcaat ggatggggc ggcaagatg 
      cttggaagc actaagact ccagcacaa aatttgcaa 
      ctgccgacc attagaatt caatacaag actgttaga 
      agtagctag ttccgtcgg tgctaaatt gttggtgcg 
      cacggtgcg cctgcgtct ctcgcgcat ctatcacgc 
      cctaggaga cgaagacca ccgtctcca ccacgaaag 
      ggtcgcctt ccgtctcca gcaggacct ggacctcat 
      gtgcggcgg ctgcggctc tccttacgg ccttgaaag 
      cgactcact tgaagctct tccctgtgc tcatatctt 
      acacgcgag atgtgccta cgatttgtt ccgaaggag 
      tagtaaaaa gaagaaacg gtcgtgtcc tgaatgcga 
      tcggactag ttctaggtt atatttgaa ttgtacaga 
      gtacaacac ttcgtacag tccggtgcc gttgtctgt 
      ctgttcttc cgacagtca ctgtacgtc gcatcaagc 
      caccagttc ggtttaaac agtaccaat gattttgct 
      cacttatca tttctgacc acacggtac aatcgctac 
      gtgaacaat cactactca tcactagaa gtagaccaa 
      aatctggta gaggtagat taagtaggt gttgaaaat 
      ttcttgggc actttcttc cacgcacta ttcaaaaat 
      ttagacggt cgatccttt ctagtccgg aatcaagtc 
      acgatattt catgtaaat acaatatgt aaggcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY