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Gene information
General information
TC009851 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9108121..9109940
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009851
    iBeetle screen iB_10950

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009851 RA
      MMEGAATPV AEGPPEEAI KPIGCSHYK RKSKFVTPC 
      CKKVYTCRF CHDEKEDHS VNRKEVTEL ICTICEKRQ 
      PVQAECQNC GIRFGKYTC LECNLFDDE DKNQFHCSG 
      CGICRIGGS DKFFHCEKC NMCLPIQLK NGHKCVENV 
      SRANCPVCL EDIHTSRIP CHIPMCGHL LHRTCFNQL 
      LKAGHYACP ICQTSLMDM TQLWKYLDN EVAHTPMPP 
      QYNCYLVQI LCKDCHKEG TVKFHVIGC KCIHCGSYN 
      TCRIKGPFK PSGRNRQNS SSQAQSLQY ENDDDGPSS 
      SSAQES
    CDS sequence
    • >TC009851 RA
      atgatggaa ggggcagct actccggtc gctgagggg 
      ccacccgaa gaggcaatt aagccaatt ggctgctcc 
      cattacaaa agaaaatcc aaattcgtg actccgtgc 
      tgcaagaaa gtgtacact tgtcggttc tgtcacgac 
      gaaaaggag gatcactca gtgaacagg aaagaagtc 
      acggagtta atctgcacg atatgcgag aaaaggcag 
      cctgttcaa gcagagtgt cagaactgt ggcatcaga 
      tttggcaaa tacacttgc ttggagtgc aatcttttc 
      gacgatgaa gacaagaac cagttccat tgctctggc 
      tgtggcatt tgcaggatt gggggctcg gacaaattt 
      ttccattgc gagaaatgc aacatgtgc ctgccaatc 
      cagctcaaa aacgggcat aaatgtgtg gagaacgta 
      tcgcgcgcc aattgtcct gtctgtctt gaggacatc 
      catacttcc agaattcca tgccatatt ccaatgtgt 
      ggccatttg ttacaccgc acttgtttc aaccaatta 
      ctcaaagcg ggacattat gcttgtccc atatgtcag 
      acgtccctt atggatatg acccagttg tggaagtat 
      cttgataat gaagttgct catacgcca atgccgcca 
      cagtataac tgttattta gtgcaaatt ttatgcaag 
      gattgtcat aaggaagga actgtaaag tttcacgtg 
      attggttgc aagtgtatt cattgcggt tcttacaat 
      acttgtcgg attaagggg ccgtttaag ccgtcaggg 
      aggaatagg caaaacagt tcaagtcaa gcacagtct 
      cttcagtat gaaaacgac gatgacggt ccatctagc 
      tcaagtgct caagaatca tga
    mRNA sequence
    • >TC009851 RA
      tgaatgaaa ttgacattt gggaggcaa tccgtgttg 
      cctacccgt ttatttatt tagttcaag atcgcctct 
      aatttattc cgtatgctg tcgtatttc taaaattag 
      tgccagaaa cagattttc tcgaattgc acagtcata 
      cggcggcgt ttaatcaaa tcccggcgg atgatggaa 
      ggggcagct actccggtc gctgagggg ccacccgaa 
      gaggcaatt aagccaatt ggctgctcc cattacaaa 
      agaaaatcc aaattcgtg actccgtgc tgcaagaaa 
      gtgtacact tgtcggttc tgtcacgac gaaaaggag 
      gatcactca gtgaacagg aaagaagtc acggagtta 
      atctgcacg atatgcgag aaaaggcag cctgttcaa 
      gcagagtgt cagaactgt ggcatcaga tttggcaaa 
      tacacttgc ttggagtgc aatcttttc gacgatgaa 
      gacaagaac cagttccat tgctctggc tgtggcatt 
      tgcaggatt gggggctcg gacaaattt ttccattgc 
      gagaaatgc aacatgtgc ctgccaatc cagctcaaa 
      aacgggcat aaatgtgtg gagaacgta tcgcgcgcc 
      aattgtcct gtctgtctt gaggacatc catacttcc 
      agaattcca tgccatatt ccaatgtgt ggccatttg 
      ttacaccgc acttgtttc aaccaatta ctcaaagcg 
      ggacattat gcttgtccc atatgtcag acgtccctt 
      atggatatg acccagttg tggaagtat cttgataat 
      gaagttgct catacgcca atgccgcca cagtataac 
      tgttattta gtgcaaatt ttatgcaag gattgtcat 
      aaggaagga actgtaaag tttcacgtg attggttgc 
      aagtgtatt cattgcggt tcttacaat acttgtcgg 
      attaagggg ccgtttaag ccgtcaggg aggaatagg 
      caaaacagt tcaagtcaa gcacagtct cttcagtat 
      gaaaacgac gatgacggt ccatctagc tcaagtgct 
      caagaatca tgaaattat tatcattag acgtcgatt 
      ccattacca ttaattaag tcaattcgg caggttaca 
      acgtattct tcgcatttc atatttaat tatattatc 
      aaatagtac tattagatg aacgtccct accaatacc 
      ctgaatatt agtatgtat atataagtt gtacatagt 
      gtacaagtt tgtgtgatt ttattttta tatgatcat 
      ttacaaata ttcaatctg ttgatgtta ttgatgttt 
      taaacaata aatagatct cctattgat tggttattg 
      ttaaattgg tttaggtta gttatatgc gattgaaca 
      aattatttg tatcatagg gagcaggtg ggtggatga 
      cctaggctg aaacccatt tcaatttta gtattgaaa 
      cgaaacaat aaaaagtca gcgaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY