Status
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Gene information
General information
TC012650 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12508074..12522708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012650
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10751

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva anterior circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012650 RA
      MMLTWGLFC VLVTLATAR NKAPLLDDG TRTRNKAAE 
      CVFGKQVRE LGSQWIPDL GVPIGVLYC MKCECVPLQ 
      KKRRIVARV QCRSIKNEC PEPTCDEPV HRPGRCCKT 
      CPGDLYDSD IIQDIVPQN VLDEEEKSS TKHYAALLT 
      ERSSLVLRN DFRLMSNNL NKNNVVATG RFTFHKKNL 
      YYSFYISDK AARPRSLQF VDSEGNILE EFVLSRAGG 
      LVNSLYQNA TRKVCGVWK RIPHDYRKR FRMEKIYVA 
      LVWGVKDQA EFTLSGQVM KYAALATEN FSSLLEPAV 
      GTMTGAGGT AIVSISTSV STSIYLAIV FNGLFTEED 
      VADVPINVT LSLDEKKYI LQEVVRVKK PHYELNMVE 
      VRSPVTPAD LRLLTRGRL VLTVSSVSK PEALRLSGS 
      VVTKVTCEL FQATLSSSS DTNRFGTSG LAWLYLNNE 
      GSLVYNVQI ENLKTTPDN TTFITLVDV STKRRTELE 
      DLTPTFDFT NGWANGTLA KLSPKVLEP LYSGNLAVN 
      VATPNDTSL VRGRLVAKP VADARDAPA PFLLKRENY 
      TLPASAVGI AWLYVDNDC CIHYDVSLS GLGSDRRLE 
      LFLELLPML APGAPVMTR YLGDFEGNQ VEGSPVTSL 
      NREEIKRLD NGVGFLKVK ENEITLLVA TVRHVMAPS 
      TCQPQNPDN DVHSIVDDS DVGPSGDCF YENKFYQQE 
      AQWTSSDNP CTTCFCENG NNKCFTMEC PEVTCPDNM 
      KLEKVPGEC CAICVNATS LVESNRNVP QKCIFNGQA 
      YSPGSRFHP FLIPTGFDS CTVCTCDAK YLEIKCKRI 
      YNENQCCKN CARVDYSMN GTYSADDEV PMLLHGVVP 
      RKEIPAKSA EQILAEGGC PNLYDARKP YVNGSEYHP 
      SIVSLGEYK CVTCKCENG KPTCWRECD KATCEKLFR 
      MKKKQRTEA GVRWNNSQC CTLRMCRKY RHKKGHRLQ 
      KS
    CDS sequence
    • >TC012650 RA
      atgatgctc acgtggggc ctcttttgc gtgctggtc 
      acgctcgcc accgccagg aacaaggcg ccgctcttg 
      gacgacggc accaggacc aggaacaag gctgcagag 
      tgcgtgttc gggaagcag gtccgcgag ctgggctcg 
      cagtggatc cccgacctc ggagtgccc atcggcgtc 
      ctctactgc atgaaatgc gagtgcgtt ccgctccag 
      aagaaacgg cggatcgtg gcgcgggtc cagtgccgg 
      agcatcaag aacgagtgc ccggagccc acctgcgac 
      gagccggtg caccggccg ggccgctgc tgcaagacg 
      tgtcctggg gatctgtac gattctgac atcatccaa 
      gacattgtc ccccaaaat gtgctcgac gaagaggag 
      aaaagcagc accaaacat tacgctgct ctgctcacc 
      gaaaggtcc tccctagtc ctgcggaac gacttcaga 
      ctcatgtcc aacaaccta aacaagaac aatgtagtg 
      gcgactgga aggttcacc ttccacaag aaaaatctc 
      tactactcg ttctatatt tccgacaaa gcggcgaga 
      cctcgctcc cttcagttc gtcgacagc gaggggaat 
      atcctcgag gagtttgtt ttgtcaagg gccgggggt 
      ttggtcaac agtttgtac caaaacgcg acccggaaa 
      gtgtgcggt gtctggaag cgcattcct cacgactac 
      cgcaaacgc ttccgaatg gaaaaaatc tacgtggca 
      ctcgtttgg ggtgtcaaa gatcaagcc gagttcact 
      ctcagtggc caagtgatg aaatacgcc gctctggct 
      actgaaaat ttcagttcg ttgctagaa ccggctgtt 
      gggactatg accggagcc ggcggtacc gctatagtg 
      tcgatttcg acgtcagtc tcaacctcc atctaccta 
      gcaattgtt tttaatgga cttttcaca gaagaagac 
      gttgcggac gtcccgatc aacgtcacc ctgtcactt 
      gacgaaaag aaatacatc ctccaggag gtggtccgt 
      gtgaaaaag ccccactat gagttaaac atggtcgag 
      gttcgttcc ccggtgact cctgctgat ctccgtcta 
      ctgacgcgc ggtcgcctc gttctgacc gtctcttct 
      gtctctaaa ccggaggct cttcgtctc agcggcagc 
      gttgtgacg aaagtaacg tgcgaactg ttccaagcc 
      acactttca agttcttcc gacaccaat cggttcgga 
      acttctggt cttgcttgg ctatatttg aacaatgaa 
      ggctctctg gtctacaac gtccaaatc gaaaatctc 
      aaaaccaca cccgataac accactttt atcacactg 
      gtggacgtt agcaccaaa cgccgcacc gagcttgaa 
      gacctcacg ccaactttt gacttcacc aacggttgg 
      gccaatggc actttggcc aaacttagc cccaaagtg 
      ctggagcct ctctactcg ggaaatttg gccgtcaat 
      gttgccacc cctaacgat actagtctc gtccgcggg 
      cgtctggtg gccaaaccc gtggccgac gctcgcgac 
      gccccagcg ccgttcttg ctgaaacgg gagaattac 
      acacttccg gcgtccgcc gttggcatc gcctggctc 
      tacgtcgac aatgactgt tgtatccac tatgatgtg 
      tccctttcc ggtctcggg tccgacagg aggttggaa 
      ctcttcttg gagttgctc ccgatgctg gcaccgggg 
      gcccccgta atgacgcgt tacttgggc gattttgaa 
      gggaaccaa gtcgaagga agtccggtc acttccctc 
      aatcgggaa gagatcaaa cggttggat aacggagtt 
      gggttcctc aaggtcaag gagaacgag atcactctt 
      ctcgtggcg acggtgcgg catgtcatg gcaccctcg 
      acttgccag ccgcagaac cccgacaac gacgtccac 
      agtatcgtt gatgactcc gatgttggt cccagtggg 
      gattgcttc tacgagaac aagttctac cagcaggaa 
      gcccagtgg acttccagt gataacccg tgcactacg 
      tgcttttgc gagaacggg aataacaaa tgcttcacc 
      atggaatgt ccggaagtc acgtgtcct gataatatg 
      aagcttgag aaggtccca ggggagtgt tgtgcgatt 
      tgtgtcaat gccactagt ttagtggaa agcaatcga 
      aatgtgcct caaaagtgt attttcaac ggtcaagct 
      tactccccg gggagtagg ttccatccg ttccttatt 
      cccactggg tttgactcg tgtacggtg tgcacgtgc 
      gacgccaag tacctcgag atcaaatgc aagaggatt 
      tacaacgag aaccagtgt tgcaagaat tgtgcccgc 
      gttgactac agcatgaac ggcacttac tccgccgat 
      gacgaagtc ccgatgctt cttcatgga gttgtgcct 
      cggaaggag atcccggcc aagagcgcc gagcagatc 
      ctggccgag ggaggctgt ccgaatctg tacgacgcg 
      aggaagccc tacgtcaac ggcagcgag taccacccc 
      tccatcgtc tcccttggg gagtacaag tgtgttact 
      tgtaaatgt gagaatggt aaaccgacg tgttggagg 
      gagtgcgac aaggccact tgcgagaag ctgttccgt 
      atgaagaag aagcagcgc actgaggcc ggcgtcagg 
      tggaacaac agccagtgt tgtacgctg cgtatgtgc 
      cggaagtac cggcacaag aagggccac cggctgcag 
      aagagttga
    mRNA sequence
    • >TC012650 RA
      ggctggcga cgcgttcgt acgtctacg tcacagtgt 
      caccttatt attacataa ataagtcgt atctgtgaa 
      gatgtattc gagataaaa ctgccacgg agtgaagtg 
      ttgtgtgtt tttttgcaa atcttgtgc gatgatgct 
      cacgtgggg cctcttttg cgtgctggt cacgctcgc 
      caccgccag gaacaaggc gccgctctt ggacgacgg 
      caccaggac caggaacaa ggctgcaga gtgcgtgtt 
      cgggaagca ggtccgcga gctgggctc gcagtggat 
      ccccgacct cggagtgcc catcggcgt cctctactg 
      catgaaatg cgagtgcgt tccgctcca gaagaaacg 
      gcggatcgt ggcgcgggt ccagtgccg gagcatcaa 
      gaacgagtg cccggagcc cacctgcga cgagccggt 
      gcaccggcc gggccgctg ctgcaagac gtgtcctgg 
      ggatctgta cgattctga catcatcca agacattgt 
      cccccaaaa tgtgctcga cgaagagga gaaaagcag 
      caccaaaca ttacgctgc tctgctcac cgaaaggtc 
      ctccctagt cctgcggaa cgacttcag actcatgtc 
      caacaacct aaacaagaa caatgtagt ggcgactgg 
      aaggttcac cttccacaa gaaaaatct ctactactc 
      gttctatat ttccgacaa agcggcgag acctcgctc 
      ccttcagtt cgtcgacag cgaggggaa tatcctcga 
      ggagtttgt tttgtcaag ggccggggg tttggtcaa 
      cagtttgta ccaaaacgc gacccggaa agtgtgcgg 
      tgtctggaa gcgcattcc tcacgacta ccgcaaacg 
      cttccgaat ggaaaaaat ctacgtggc actcgtttg 
      gggtgtcaa agatcaagc cgagttcac tctcagtgg 
      ccaagtgat gaaatacgc cgctctggc tactgaaaa 
      tttcagttc gttgctaga accggctgt tgggactat 
      gaccggagc cggcggtac cgctatagt gtcgatttc 
      gacgtcagt ctcaacctc catctacct agcaattgt 
      ttttaatgg acttttcac agaagaaga cgttgcgga 
      cgtcccgat caacgtcac cctgtcact tgacgaaaa 
      gaaatacat cctccagga ggtggtccg tgtgaaaaa 
      gccccacta tgagttaaa catggtcga ggttcgttc 
      cccggtgac tcctgctga tctccgtct actgacgcg 
      cggtcgcct cgttctgac cgtctcttc tgtctctaa 
      accggaggc tcttcgtct cagcggcag cgttgtgac 
      gaaagtaac gtgcgaact gttccaagc cacactttc 
      aagttcttc cgacaccaa tcggttcgg aacttctgg 
      tcttgcttg gctatattt gaacaatga aggctctct 
      ggtctacaa cgtccaaat cgaaaatct caaaaccac 
      acccgataa caccacttt tatcacact ggtggacgt 
      tagcaccaa acgccgcac cgagcttga agacctcac 
      gccaacttt tgacttcac caacggttg ggccaatgg 
      cactttggc caaacttag ccccaaagt gctggagcc 
      tctctactc gggaaattt ggccgtcaa tgttgccac 
      ccctaacga tactagtct cgtccgcgg gcgtctggt 
      ggccaaacc cgtggccga cgctcgcga cgccccagc 
      gccgttctt gctgaaacg ggagaatta cacacttcc 
      ggcgtccgc cgttggcat cgcctggct ctacgtcga 
      caatgactg ttgtatcca ctatgatgt gtccctttc 
      cggtctcgg gtccgacag gaggttgga actcttctt 
      ggagttgct cccgatgct ggcaccggg ggcccccgt 
      aatgacgcg ttacttggg cgattttga agggaacca 
      agtcgaagg aagtccggt cacttccct caatcggga 
      agagatcaa acggttgga taacggagt tgggttcct 
      caaggtcaa ggagaacga gatcactct tctcgtggc 
      gacggtgcg gcatgtcat ggcaccctc gacttgcca 
      gccgcagaa ccccgacaa cgacgtcca cagtatcgt 
      tgatgactc cgatgttgg tcccagtgg ggattgctt 
      ctacgagaa caagttcta ccagcagga agcccagtg 
      gacttccag tgataaccc gtgcactac gtgcttttg 
      cgagaacgg gaataacaa atgcttcac catggaatg 
      tccggaagt cacgtgtcc tgataatat gaagcttga 
      gaaggtccc aggggagtg ttgtgcgat ttgtgtcaa 
      tgccactag tttagtgga aagcaatcg aaatgtgcc 
      tcaaaagtg tattttcaa cggtcaagc ttactcccc 
      ggggagtag gttccatcc gttccttat tcccactgg 
      gtttgactc gtgtacggt gtgcacgtg cgacgccaa 
      gtacctcga gatcaaatg caagaggat ttacaacga 
      gaaccagtg ttgcaagaa ttgtgcccg cgttgacta 
      cagcatgaa cggcactta ctccgccga tgacgaagt 
      cccgatgct tcttcatgg agttgtgcc tcggaagga 
      gatcccggc caagagcgc cgagcagat cctggccga 
      gggaggctg tccgaatct gtacgacgc gaggaagcc 
      ctacgtcaa cggcagcga gtaccaccc ctccatcgt 
      ctcccttgg ggagtacaa gtgtgttac ttgtaaatg 
      tgagaatgg taaaccgac gtgttggag ggagtgcga 
      caaggccac ttgcgagaa gctgttccg tatgaagaa 
      gaagcagcg cactgaggc cggcgtcag gtggaacaa 
      cagccagtg ttgtacgct gcgtatgtg ccggaagta 
      ccggcacaa gaagggcca ccggctgca gaagagttg 
      aatcggtgg ccgaggtta cgtcactag ttagactag 
      actgaccgt tgtacatgc attggaact ttaaggact 
      tcaagtcca gttaccgtt taccaaaga agactgaga 
      cagtaggat ggatggagc tgtcgtatt tattcgagt 
      tagcgtacg tcagttttt agattaggt tttgaacat 
      ggcgcagtc aactaattc gggttcttt gacagatta 
      tttatttat ttttttata tgtagtttt cggcgggat 
      attacttaa ttacttgac tgggtcgca tcattacta 
      tttatttga cattgtata agttattcc atagttgta 
      tcaggggtt cctgactgg aacctaata ctcactaga 
      gacgaaggc tgataggga cgcaggatt tggacagga 
      accgacctc tatgtataa tatttatac tcgatattc 
      ttcaagttt tagtttttg ggttcttgt gcatattcc 
      ttcttgtaa attgtatca atatcctcg taggacata 
      tcatttggt gccatcccc caaaattct ttaggtact 
      taaaccaca ctcgttacg agcatttaa atgtaatcg 
      gaatctccc ttgcaataa tacagtatt ttgctaaat 
      tatttttag gttacgtaa cagttttgt gtgaatgtg 
      atttacgga cgtgttaag ttatatatc agttaaata 
      aatttattg tacatagtt tttttgctg tatgattat 
      ataaaaacc atacgcttt gtatattag tttcttttt 
      ctagtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY