Status
Please wait ...
Gene information
General information
TC009045 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8133833..8143750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009045
      iBeetle screen iB_10750

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009045 RA
        MPPVRWKPL PALVVCVFH LVFYTDAQI RQDTVSMTA 
        DGWKPIVGT RRPGYYEFK SDSSDIYKY EVFSTPPPT 
        NTALPPTAS AGPEQKPFR GSPKPANKP TFEGNKFPG 
        RKIPHQKHP QHNPYFTLP NYDSHGPHI PLGAVIHTR 
        PSQIGHIKP LATSIKPFI QISPSSKRN IKPIRIQTS 
        TNNYRDDIF TASATSGTY THFKTPTSN IRTRETIDL 
        GLPSANHYS INQPHAHIE FANRFALPP TSESVFQQY 
        NNPAIPAFI FSKHPQSND VNYQFNTQE DFTRNLVPP 
        PQIYRNKET GTEKVKEKG KVQENVVLQ VPPPFQFQK 
        GNDPVQVQV TREKIQEFH NNVPPGFDG QYVEYDFSG 
        VRQPTVPSK PVYEVTEGK WEEPPSQRP IRTRKPSQP 
        KRRPEITPE VPVFLPTPY KPDGQSGII PTSPTQSEV 
        STIFTKLSK LQREKAYTT PSPNDYNVK EVSTHYPVF 
        GKPVFANPP PSDEISNDI TTPHEEVVT TTKEPQRVR 
        PHRRRRPES RRTTTTTTT EQPEVVESY DSQRTQENE 
        AYSQSVETE RPVRIRRPS RYRTTTPPS ETSDVTTKP 
        SRGRNRYRT RNGENTSYE SIRKRIRPL RVETTPPVS 
        EETYDRSSE SEAVASEKS EEIEDNPKA ETVELELPG 
        RHYITEPPK KVRYETIRP FIVITTTEK APTTNLVVP 
        ENEVDSTSL ASVEETTTP APTTTTTTT TTQSPNRIR 
        GRPLKFDNS NRPRFSVKD YRQKLSQYS TSTTTPEPH 
        RSTDNPRIR LPSRLRRPT TSTTANNEE ESTEPVRSR 
        FVPKDPRHS GTTEDTNAA IITEKNVKS VNTRLRPFG 
        RYKSTTAAT TSTPKVSIK PNLFNRKRP PMISLKSRI 
        YGKYKNNTE TTTEKVTTT EENEVEEST FEYDDGEST 
        TERSKVITE DSLEVDTTT VGDISKIDA NLYSARVSD 
        LTSSAKNDY NTFKSVAPT SRRIPNYFT IATDDPILP 
        IEAFFPNIK DKIREKDS
      CDS sequence
      • >TC009045 RA
        atgccaccg gtgagatgg aaaccactg ccagctcta 
        gtcgtctgt gtgttccat ctggtgttt tacacagat 
        gctcagatt agacaggat actgtgtcc atgacggcc 
        gatgggtgg aaacccatt gttgggacc cgacgaccg 
        gggtactac gagtttaag tcggatagt agcgatatt 
        tacaagtat gaggttttt tcgactccg ccaccgact 
        aacacggcc ctgcctccg actgccagt gcagggccg 
        gagcagaag ccgttccgg gggtcgccc aaaccggcg 
        aacaagccc acatttgaa gggaataaa tttccagga 
        cgaaaaatt ccgcaccag aagcacccc caacacaac 
        ccctacttt actctcccc aattacgac tcccacggc 
        ccccacatc cccctgggt gccgtaatt cacacccgc 
        cccagccag atcggccat atcaagccc cttgcaact 
        tccatcaaa ccctttata caaatatca cccagcagc 
        aaacgaaac atcaaacca atccggata caaacatca 
        acaaacaac taccgggat gatattttt acagcctcg 
        gcaacttcc ggcacctac actcatttt aaaacaccg 
        accagtaac atccggaca cgtgaaaca atagacttg 
        ggccttccg agcgccaat cactattcg ataaaccaa 
        ccccacgcc cacattgag ttcgccaat cggtttgct 
        ttgcccccc accagcgaa agcgtcttc caacagtac 
        aacaatccg gcaatcccg gcttttatc ttctcaaaa 
        cacccccaa agtaatgac gttaattat cagttcaac 
        actcaggag gatttcacc cggaatttg gtaccgccg 
        cctcagatt taccgcaat aaggagacc gggactgaa 
        aaagtgaaa gaaaaggga aaagttcag gaaaatgtt 
        gttctgcaa gtaccacca cccttccag ttccagaag 
        ggcaatgat ccggttcaa gttcaagtc actagagag 
        aagatccaa gagtttcac aataatgta ccacctggg 
        tttgatggg caatacgtc gagtatgat ttttcggga 
        gtgagacag cctacagta ccatcgaaa cctgtatat 
        gaggtaact gagggtaaa tgggaggaa ccaccgtcc 
        caaagacca atacgaacg agaaaacct agccaacct 
        aagagacgt cccgaaatc actccagaa gttcccgtt 
        tttctacca acgccttac aaacctgat gggcaaagc 
        ggaattatc ccgacttca cccacccaa agtgaggta 
        tcaacaatt ttcacgaaa ttatcaaaa ttgcaacga 
        gaaaaagcc tacacaaca cctagtccc aacgattac 
        aatgtgaaa gaagtttca acacattat cccgttttt 
        ggaaagcct gtttttgca aatccgcca ccttccgac 
        gaaatttca aacgatata acgacacca catgaagaa 
        gttgtgacc acaaccaaa gaaccacaa cgagtgagg 
        ccacacaga aggcgcagg cctgaatcc agacgtaca 
        acgaccaca acaacgaca gaacaacct gaagttgtt 
        gaaagttat gattcccaa aggacgcaa gaaaacgaa 
        gcttattca cagtcggtt gaaaccgaa agaccggtc 
        agaataaga cgaccgagt agatacaga acaacaaca 
        ccaccaagt gagactagc gatgtcact acgaaacct 
        agtcgaggg agaaacagg tacagaacc agaaatggt 
        gaaaataca agttatgaa tcaataaga aagagaata 
        aggccctta agagtggaa acaacgcca ccagtctct 
        gaagaaact tacgacaga tcgtccgag tctgaagct 
        gtggcgtct gaaaaaagt gaagaaatt gaagacaat 
        cctaaggct gaaacggtt gaattggaa ttgcctggg 
        cgccactac atcacagag cctcccaaa aaagtcagg 
        tatgaaacc ataaggccg tttattgtg attacaacc 
        actgagaaa gcgcccaca acaaacttg gttgttcct 
        gagaatgaa gtcgattcg acttctctg gccagtgtt 
        gaagaaaca acaacacca gcgccaacg acaacaaca 
        acaacaaca acgactcaa agtccgaac aggattaga 
        ggacgtccg cttaaattt gacaattcg aacaggccc 
        aggtttagt gtcaaagac tacagacaa aaactgagt 
        caatacagc acaagtaca acgactcct gagccacac 
        agatcaact gataatccc aggattagg cttccaagt 
        aggttgcgc agacctaca acttcaacc actgccaac 
        aatgaagag gaaagtacc gaaccggtg cgatccagg 
        tttgttcct aaagacccc aggcattct gggacaact 
        gaggacaca aacgctgcg ataattact gagaaaaat 
        gtgaaaagt gttaataca aggctacgg ccttttgga 
        cgttacaag tcgaccact gccgccaca acatcgaca 
        ccaaaagtg tcaatcaaa ccgaatctt ttcaacagg 
        aagcgaccc ccaatgatc agtttaaag agtcgcatt 
        tacgggaaa tacaaaaat aataccgaa accacaact 
        gagaaagtg acaacaacg gaggaaaat gaagttgag 
        gagagtact tttgaatat gacgatggg gagtctaca 
        actgagaga agcaaagtc atcaccgag gacagtcta 
        gaggtggat acaaccact gttggtgac atttccaaa 
        atcgatgct aatttatac tcggcgcga gtctccgat 
        ttgacttcg tcggctaaa aacgactac aatacattc 
        aaatcggtg gcccccacg agtcgaaga atcccgaat 
        tatttcacg atagccacc gacgaccca attctgcca 
        attgaggcg tttttccca aacatcaag gataaaatc 
        agggagaag gattcgtaa
      mRNA sequence
      • >TC009045 RA
        ctcagcccc cgaaagtga aattagttt tcgtggtcg 
        gcccttggc tcgcgtttg gaatacttg atcagactt 
        ggcaagtgg atcgtcgtt ctagatttt gctcgaagg 
        cctactttc ttcttcttc ttgcacaca atgagctgt 
        tattacgag ttcggtgca ctgtcatta cgcaccgta 
        tttatccaa caatgtcaa aatatcgag accggatcg 
        atttcgggg gctttcccc cgcgccata cggccggcg 
        ataatttca ctgccgact aattagctt gatggcggg 
        tttctaaaa gcggtttcg ggatgaaaa gggaatcgc 
        acgtgtgaa agtcggggg gctctggcg cgtgccacg 
        agaggaggg gcctccact gtaccagaa gcccggctt 
        tgatagaca gcagttcgc tcatcgcga ttgcaaagt 
        gtgtagtct tcgtcttga gcattttca agaccgaga 
        tttcgcgcc aaagcggaa cagtttccg gtcaagaga 
        agcgagtgg tttttcaat ttgcggttt agttttttt 
        acagctgca aaatgccac cggtgagat ggaaaccac 
        tgccagctc tagtcgtct gtgtgttcc atctggtgt 
        tttacacag atgctcaga ttagacagg atactgtgt 
        ccatgacgg ccgatgggt ggaaaccca ttgttggga 
        cccgacgac cggggtact acgagttta agtcggata 
        gtagcgata tttacaagt atgaggttt tttcgactc 
        cgccaccga ctaacacgg ccctgcctc cgactgcca 
        gtgcagggc cggagcaga agccgttcc gggggtcgc 
        ccaaaccgg cgaacaagc ccacatttg aagggaata 
        aatttccag gacgaaaaa ttccgcacc agaagcacc 
        cccaacaca acccctact ttactctcc ccaattacg 
        actcccacg gcccccaca tccccctgg gtgccgtaa 
        ttcacaccc gccccagcc agatcggcc atatcaagc 
        cccttgcaa cttccatca aacccttta tacaaatat 
        cacccagca gcaaacgaa acatcaaac caatccgga 
        tacaaacat caacaaaca actaccggg atgatattt 
        ttacagcct cggcaactt ccggcacct acactcatt 
        ttaaaacac cgaccagta acatccgga cacgtgaaa 
        caatagact tgggccttc cgagcgcca atcactatt 
        cgataaacc aaccccacg cccacattg agttcgcca 
        atcggtttg ctttgcccc ccaccagcg aaagcgtct 
        tccaacagt acaacaatc cggcaatcc cggctttta 
        tcttctcaa aacaccccc aaagtaatg acgttaatt 
        atcagttca acactcagg aggatttca cccggaatt 
        tggtaccgc cgcctcaga tttaccgca ataaggaga 
        ccgggactg aaaaagtga aagaaaagg gaaaagttc 
        aggaaaatg ttgttctgc aagtaccac cacccttcc 
        agttccaga agggcaatg atccggttc aagttcaag 
        tcactagag agaagatcc aagagtttc acaataatg 
        taccacctg ggtttgatg ggcaatacg tcgagtatg 
        atttttcgg gagtgagac agcctacag taccatcga 
        aacctgtat atgaggtaa ctgagggta aatgggagg 
        aaccaccgt cccaaagac caatacgaa cgagaaaac 
        ctagccaac ctaagagac gtcccgaaa tcactccag 
        aagttcccg tttttctac caacgcctt acaaacctg 
        atgggcaaa gcggaatta tcccgactt cacccaccc 
        aaagtgagg tatcaacaa ttttcacga aattatcaa 
        aattgcaac gagaaaaag cctacacaa cacctagtc 
        ccaacgatt acaatgtga aagaagttt caacacatt 
        atcccgttt ttggaaagc ctgtttttg caaatccgc 
        caccttccg acgaaattt caaacgata taacgacac 
        cacatgaag aagttgtga ccacaacca aagaaccac 
        aacgagtga ggccacaca gaaggcgca ggcctgaat 
        ccagacgta caacgacca caacaacga cagaacaac 
        ctgaagttg ttgaaagtt atgattccc aaaggacgc 
        aagaaaacg aagcttatt cacagtcgg ttgaaaccg 
        aaagaccgg tcagaataa gacgaccga gtagataca 
        gaacaacaa caccaccaa gtgagacta gcgatgtca 
        ctacgaaac ctagtcgag ggagaaaca ggtacagaa 
        ccagaaatg gtgaaaata caagttatg aatcaataa 
        gaaagagaa taaggccct taagagtgg aaacaacgc 
        caccagtct ctgaagaaa cttacgaca gatcgtccg 
        agtctgaag ctgtggcgt ctgaaaaaa gtgaagaaa 
        ttgaagaca atcctaagg ctgaaacgg ttgaattgg 
        aattgcctg ggcgccact acatcacag agcctccca 
        aaaaagtca ggtatgaaa ccataaggc cgtttattg 
        tgattacaa ccactgaga aagcgccca caacaaact 
        tggttgttc ctgagaatg aagtcgatt cgacttctc 
        tggccagtg ttgaagaaa caacaacac cagcgccaa 
        cgacaacaa caacaacaa caacgactc aaagtccga 
        acaggatta gaggacgtc cgcttaaat ttgacaatt 
        cgaacaggc ccaggttta gtgtcaaag actacagac 
        aaaaactga gtcaataca gcacaagta caacgactc 
        ctgagccac acagatcaa ctgataatc ccaggatta 
        ggcttccaa gtaggttgc gcagaccta caacttcaa 
        ccactgcca acaatgaag aggaaagta ccgaaccgg 
        tgcgatcca ggtttgttc ctaaagacc ccaggcatt 
        ctgggacaa ctgaggaca caaacgctg cgataatta 
        ctgagaaaa atgtgaaaa gtgttaata caaggctac 
        ggccttttg gacgttaca agtcgacca ctgccgcca 
        caacatcga caccaaaag tgtcaatca aaccgaatc 
        ttttcaaca ggaagcgac ccccaatga tcagtttaa 
        agagtcgca tttacggga aatacaaaa ataataccg 
        aaaccacaa ctgagaaag tgacaacaa cggaggaaa 
        atgaagttg aggagagta cttttgaat atgacgatg 
        gggagtcta caactgaga gaagcaaag tcatcaccg 
        aggacagtc tagaggtgg atacaacca ctgttggtg 
        acatttcca aaatcgatg ctaatttat actcggcgc 
        gagtctccg atttgactt cgtcggcta aaaacgact 
        acaatacat tcaaatcgg tggccccca cgagtcgaa 
        gaatcccga attatttca cgatagcca ccgacgacc 
        caattctgc caattgagg cgtttttcc caaacatca 
        aggataaaa tcagggaga aggattcgt aaaccagtt 
        ttcgtagcc aactgggta acagcattt tccgggggc 
        gaagaaaaa gacagtaaa atg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY