Status
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Gene information
General information
TC007746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 517298..522440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007746
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007746 RA
      MSVPGSTSG VNSRRQKRM GPAPFASFE DLSDEVRSR 
      LESPEDKNP AGSVVRLPG IREDATPID HPGISLLGA 
      MPLSSPQKL TPRIDISRA SSSSHHDSR DSTPERELF 
      DHEAPKLGL GFREEGALD LRSSTEELD FQDPQEVKK 
      KPTKPQSPE EISHHQRKD SQSSEIGLL CISGRTSRL 
      SSIGSQGSG HSRLSNASH LSVISNQSG LSRSPSPHK 
      MLLETSFCG TKPPENAPN NKSQTGTKI ETEVLEKVL 
      LARKHDPTQ AVLAEGIAV PPPKPKRVL QKSDITIKV 
      DEPSNNNIP RKFIAKSGV EYIYIPLKG PLPSQPETT 
      TTNTKPKLR EVKSATIQP KTKTPTKPK PKSASPSPK 
      REDEPKYIR IKLKPDHMY SNSQEEESK PETLDLQPE 
      PKPIESPKP STATPSPSL SRKSSFASL FRSKETIIS 
      PESPTGCKR KSLGGILSE SLKERSRSK SRERDLSNE 
      SIGKQKSVF SRFRKKKDE TTSSSEVLP NFDAIAKVE 
      FKFNDNRHE LESDSVRIP LHSPTFYED RTIVTSSQD 
      SQETVIEVN RTEMEVQVE SNITVNGGG GAKSEPQRQ 
      SSTSSENVV FSTKLGSNN EIFSTKLPK DKAKEPEIS 
      SAPEDVSSE SERDSEAEL PKKKDLKLE LDYVEPERK 
      ELVLQDSYD DELPYVPTT LPQERSAAL PIIPIKQRS 
      TFEMKTHPI ERPRSTTPI NPSCLEEYC EDVMTTINT 
      IEKLKISLP RNESVEKTV KSPRKPISN TTNWFEFAE 
      KGINTGRRS SLTQDETPP PLPPKGVQK EWINFEEIP 
      ERRKPPKRI QTLPSRGHI EVSESVLQD NVVYNYVNP 
      EECKCECHE MRERKKGCG GVQEDELPL LDDEQEEKE 
      GERLKLDVA VADRRSIVS DSSVDLSAA VEPPEANGS 
      VKLRTPFTS DLGVSSNRS SIVSQDEPE SPDSGSPNA 
      FPKPLLG
    CDS sequence
    • >TC007746 RA
      atgtcggta cctggaagt acctctggt gttaattca 
      cgacgtcag aagcgcatg ggtcctgcc cctttcgcc 
      tcttttgaa gacctctca gacgaggtg agatcccgt 
      ctggagagt cccgaagac aaaaacccg gccggatcc 
      gtggtcaga cttccaggt atccgagaa gacgcgaca 
      ccaatcgac caccctggc ataagtctc ttaggtgca 
      atgccactc tccagcccc cagaaacta acaccaaga 
      atcgacatt tcccgagcc agtagttca tcacatcat 
      gacagtcga gacagcact ccagaacgt gaacttttc 
      gatcatgaa gccccaaaa ttaggtcta ggctttcgg 
      gaagaaggc gcccttgat ttacgatca tcaactgaa 
      gaattggac ttccaagac ccacaagaa gtgaagaaa 
      aaacccacc aaaccacaa tcacccgaa gaaataagc 
      catcatcaa agaaaagat tcacaaagt tccgaaatt 
      ggtttattg tgtatttct ggacgtacg tcacgactt 
      tcaagtatc ggaagtcaa ggttctgga catagtcgc 
      ttatcaaat gcgagtcat ctttccgtt atttccaac 
      caatcagga ctgtcacga tcaccatcg cctcataaa 
      atgttactc gagacgtcg ttttgtggc acgaaacca 
      ccggaaaac gcaccgaat aacaagagc caaaccggg 
      accaaaatc gagacggaa gtgctcgaa aaagtgctt 
      cttgcacgc aaacatgac ccgactcag gccgttctc 
      gccgaaggg attgcggta ccacctcct aaacccaaa 
      cgtgtgcta caaaaaagt gatattaca attaaagtg 
      gacgaacca agcaataat aacattccg cgaaaattt 
      attgcaaaa agtggagtg gaatacatt tatatccca 
      ctcaaaggc cctctccca tcccaacct gaaacaacc 
      accaccaac accaaacca aaactccgc gaagttaaa 
      tcagccaca atccaacct aaaaccaaa acaccaacc 
      aaacctaaa ccgaaatca gcctcgcct tcaccgaaa 
      cgcgaagac gaaccaaaa tacatccga atcaaacta 
      aaacctgac cacatgtac agcaacagc caagaggag 
      gagtccaaa cctgaaact ttggactta caacctgaa 
      ccaaaacca atcgaaagt ccaaaacca tcaacagca 
      acaccatca ccttcactc tcccggaaa agctcgttc 
      gcttcactt ttccggagc aaagaaacg attataagt 
      ccggaatcg ccaaccggt tgcaaacga aaatcactc 
      ggcggtatt ttaagcgag agtttgaaa gaacgaagt 
      cgttcgaaa agccgcgaa cgtgattta tccaacgaa 
      agcatcggc aagcaaaag tcggtcttt tcgcgtttt 
      cgcaaaaaa aaggacgaa acgacgagt tcaagtgaa 
      gttttgccg aatttcgac gcaattgcc aaagtagaa 
      tttaaattt aatgataat agacacgaa ttagaaagc 
      gattcggtt cgaattccc ttgcattcg cccacgttt 
      tacgaagat cgaaccatt gtaacttcg agtcaagac 
      agtcaggaa actgtgatt gaagtcaat cgaacggaa 
      atggaagta caagtcgaa agcaatatt acggtgaat 
      ggtggtggt ggtgccaaa agcgagcca caaagacag 
      agtagtaca agtagtgag aatgtggtg ttttcaacg 
      aaacttgga agtaataat gagattttc tcgaccaag 
      ttgcccaaa gataaggcg aaagaaccg gagattagc 
      tcggcacct gaggacgtt tcgtccgaa tcggaacga 
      gactcggaa gctgaactg ccgaaaaaa aaggatttg 
      aagctggaa ctggattat gtggagccg gaacggaaa 
      gaactggtc ttgcaagac tcctatgac gacgagctc 
      ccctacgtg cccacaacc ctccctcag gagcgttcg 
      gccgccctc cccatcatc cccatcaag cagcgttca 
      accttcgaa atgaagacc caccccatc gaacgcccc 
      cgttccacc acccccatc aaccccagc tgcctcgaa 
      gagtactgt gaagacgtc atgacaacc atcaacacc 
      atcgaaaaa ctcaaaata tctctgcca cgaaacgaa 
      tcagttgaa aaaactgtc aaatcaccg cgaaaacca 
      atcagcaac acaaccaat tggtttgaa tttgccgag 
      aaagggatt aacacagga aggcgttcc tcgttgact 
      caggacgag acgccgcct ccactgccc ccgaaaggg 
      gtccaaaag gagtggatt aattttgag gaaatcccc 
      gaacggaga aaaccaccg aaacggatt caaacgtta 
      ccgtcacgt ggccatatt gaagtttct gagagtgtt 
      ttacaggat aatgttgtg tataattat gtgaatccc 
      gaggagtgc aagtgcgag tgtcatgaa atgagggag 
      aggaaaaag ggctgtgga ggggtgcag gaggatgag 
      ctgccctta ttggatgat gaacaggag gaaaaggag 
      ggggagagg cttaagttg gatgttgcg gttgctgat 
      cggagaagt atagtaagt gactcttcg gtagattta 
      agtgcagct gtagaacct ccggaagcg aacggcagt 
      gtcaaatta cgaactcca tttactagt gatttagga 
      gtgtcgagt aacagatcc agtattgta tcccaggat 
      gaacctgaa tcaccagat tccggatcg ccaaatgcc 
      tttcctaaa cctttatta gggtaa
    mRNA sequence
    • >TC007746 RA
      taacccagt aaatcacca ccaaataat aacatgtcg 
      gtacctgga agtacctct ggtgttaat tcacgacgt 
      cagaagcgc atgggtcct gcccctttc gcctctttt 
      gaagacctc tcagacgag gtgagatcc cgtctggag 
      agtcccgaa gacaaaaac ccggccgga tccgtggtc 
      agacttcca ggtatccga gaagacgcg acaccaatc 
      gaccaccct ggcataagt ctcttaggt gcaatgcca 
      ctctccagc ccccagaaa ctaacacca agaatcgac 
      atttcccga gccagtagt tcatcacat catgacagt 
      cgagacagc actccagaa cgtgaactt ttcgatcat 
      gaagcccca aaattaggt ctaggcttt cgggaagaa 
      ggcgccctt gatttacga tcatcaact gaagaattg 
      gacttccaa gacccacaa gaagtgaag aaaaaaccc 
      accaaacca caatcaccc gaagaaata agccatcat 
      caaagaaaa gattcacaa agttccgaa attggttta 
      ttgtgtatt tctggacgt acgtcacga ctttcaagt 
      atcggaagt caaggttct ggacatagt cgcttatca 
      aatgcgagt catctttcc gttatttcc aaccaatca 
      ggactgtca cgatcacca tcgcctcat aaaatgtta 
      ctcgagacg tcgttttgt ggcacgaaa ccaccggaa 
      aacgcaccg aataacaag agccaaacc gggaccaaa 
      atcgagacg gaagtgctc gaaaaagtg cttcttgca 
      cgcaaacat gacccgact caggccgtt ctcgccgaa 
      gggattgcg gtaccacct cctaaaccc aaacgtgtg 
      ctacaaaaa agtgatatt acaattaaa gtggacgaa 
      ccaagcaat aataacatt ccgcgaaaa tttattgca 
      aaaagtgga gtggaatac atttatatc ccactcaaa 
      ggccctctc ccatcccaa cctgaaaca accaccacc 
      aacaccaaa ccaaaactc cgcgaagtt aaatcagcc 
      acaatccaa cctaaaacc aaaacacca accaaacct 
      aaaccgaaa tcagcctcg ccttcaccg aaacgcgaa 
      gacgaacca aaatacatc cgaatcaaa ctaaaacct 
      gaccacatg tacagcaac agccaagag gaggagtcc 
      aaacctgaa actttggac ttacaacct gaaccaaaa 
      ccaatcgaa agtccaaaa ccatcaaca gcaacacca 
      tcaccttca ctctcccgg aaaagctcg ttcgcttca 
      cttttccgg agcaaagaa acgattata agtccggaa 
      tcgccaacc ggttgcaaa cgaaaatca ctcggcggt 
      attttaagc gagagtttg aaagaacga agtcgttcg 
      aaaagccgc gaacgtgat ttatccaac gaaagcatc 
      ggcaagcaa aagtcggtc ttttcgcgt tttcgcaaa 
      aaaaaggac gaaacgacg agttcaagt gaagttttg 
      ccgaatttc gacgcaatt gccaaagta gaatttaaa 
      tttaatgat aatagacac gaattagaa agcgattcg 
      gttcgaatt cccttgcat tcgcccacg ttttacgaa 
      gatcgaacc attgtaact tcgagtcaa gacagtcag 
      gaaactgtg attgaagtc aatcgaacg gaaatggaa 
      gtacaagtc gaaagcaat attacggtg aatggtggt 
      ggtggtgcc aaaagcgag ccacaaaga cagagtagt 
      acaagtagt gagaatgtg gtgttttca acgaaactt 
      ggaagtaat aatgagatt ttctcgacc aagttgccc 
      aaagataag gcgaaagaa ccggagatt agctcggca 
      cctgaggac gtttcgtcc gaatcggaa cgagactcg 
      gaagctgaa ctgccgaaa aaaaaggat ttgaagctg 
      gaactggat tatgtggag ccggaacgg aaagaactg 
      gtcttgcaa gactcctat gacgacgag ctcccctac 
      gtgcccaca accctccct caggagcgt tcggccgcc 
      ctccccatc atccccatc aagcagcgt tcaaccttc 
      gaaatgaag acccacccc atcgaacgc ccccgttcc 
      accaccccc atcaacccc agctgcctc gaagagtac 
      tgtgaagac gtcatgaca accatcaac accatcgaa 
      aaactcaaa atatctctg ccacgaaac gaatcagtt 
      gaaaaaact gtcaaatca ccgcgaaaa ccaatcagc 
      aacacaacc aattggttt gaatttgcc gagaaaggg 
      attaacaca ggaaggcgt tcctcgttg actcaggac 
      gagacgccg cctccactg cccccgaaa ggggtccaa 
      aaggagtgg attaatttt gaggaaatc cccgaacgg 
      agaaaacca ccgaaacgg attcaaacg ttaccgtca 
      cgtggccat attgaagtt tctgagagt gttttacag 
      gataatgtt gtgtataat tatgtgaat cccgaggag 
      tgcaagtgc gagtgtcat gaaatgagg gagaggaaa 
      aagggctgt ggaggggtg caggaggat gagctgccc 
      ttattggat gatgaacag gaggaaaag gagggggag 
      aggcttaag ttggatgtt gcggttgct gatcggaga 
      agtatagta agtgactct tcggtagat ttaagtgca 
      gctgtagaa cctccggaa gcgaacggc agtgtcaaa 
      ttacgaact ccatttact agtgattta ggagtgtcg 
      agtaacaga tccagtatt gtatcccag gatgaacct 
      gaatcacca gattccgga tcgccaaat gcctttcct 
      aaaccttta ttagggtaa taaaaccaa tttgattta 
      attaattaa aaaaccgac aagtgtgac cttttatta 
      aaatttgga tgttctaga ataaaatca atcatcgcg 
      cccgacccc tcacaataa aagccttgt atatgattg 
      tgtgtacgt aggcctatc tcttgtcca aaaactgtt 
      attgtactt ttcttgtta cctcttgga cacaaaaat 
      ttattgtta cttatcaag tgttgaccg tttctttct 
      gattaaatt atatcatgt tgaactata cggtaacaa 
      gactttgct tgtttataa ggacgttgg ttaactcac 
      gaatactat tgtacagta atattcagt aataaaata 
      tacgtgttg aaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY