Status
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Gene information
General information
TC014271 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8257065..8260859
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014271
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10739

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014271 RA
      MRRKSARNA QKASPEKAL KETETPPKT RRSRRRKRS 
      PSKSSESED EVLTITKRA PLEATPDTT TKSHTPSDE 
      ETCDEPVWK VTSSEDTPK GEIQKLKIC LTRPSPDSP 
      DKSKTRRSK SNSQTEDDA TSSEDKSST RRTRSRGSP 
      LVRNDGLSS QDGDEEVEE KKGKGKSKG KHKSKIEKS 
      PHLSEPEIQ PNNEISENV QVTPQSPSI DKRVDNEND 
      SKVDADDKN INESVQRQE NVNDVDKEQ LGNKTESQD 
      DESDDEPLS IIKKNVSVD KPVTENKQP ETTNEPSEH 
      QEPDQEVQT QIDVKQDQV EEQAPEKPE EIAITEVET 
      ETPEDKPSE IAITHEESS KETEAEKAE DISASLNET 
      QDVEMASVE EKSEQTTAI SEDKPEMEE PQVSESIAD 
      SENVEVCQQ PPQKTPEVP VESTEVLKE SARIEKSPR 
      DNKDRHRSP RRQLRRSND YAKPDSKEE PVNSGPIKP 
      FKTKHSWER SLSSAPSLL KVDLDSIQI VCPTIEFPE 
      EPDLSLELE KERRKSVTE TSGKKLERK VSVEYKSDT 
      SERPSFEKQ NSVNSSTQD GEDNSNIIA FNRKISIVD 
      DTASKLKPP PSPAKNPVS NILYITNLV RPFTVKQLK 
      ELLERTGKV QEDGFWTDR IKSKCYVQY ETQEEAEAT 
      RNALHGVQW PIGNGKKLI IDFATAEDM ENAKNPPAP 
      PPAPVPDTN VSTKENLEP AESKHDKAS KEVEKKRPE 
      GPVREWDIG KKDVRRDRS RSRERSRKH RRTPSPSDD 
      FITRKQRKA EDSVPQKLM DDLFLKTKA TPSIYWQPL 
      SPEEISMKQ QQRLIRMEE HKRRMEENS RMKGREMGR 
      GAFRRRYD
    CDS sequence
    • >TC014271 RA
      atgcggcgt aaaagtgcc cgtaacgcc cagaaagcg 
      tctcctgag aaagcgctc aaagaaacg gaaactcct 
      ccgaagacc cgtcgatcg cggcgtcgg aaaagatca 
      ccatcgaaa tcgagcgaa tctgaagat gaagttctg 
      acaataaca aagcgtgcc ccgcttgaa gctaccccc 
      gatacaaca actaagagt cacactccg agtgacgag 
      gagacttgc gatgaaccg gtgtggaaa gtgacctct 
      tctgaggat actcccaag ggggagatc cagaagcta 
      aagatttgt ctgacaagg ccttccccg gattcacct 
      gataaaagc aagacacgt cgcagtaag tctaatagt 
      cagactgaa gacgacgcg acgagttct gaggataaa 
      tcgtcaaca agacgaact cgctcacgg ggatctcca 
      cttgttaga aacgacggt ttgtctagt caagacggt 
      gatgaagaa gtggaagag aagaagggt aaaggtaag 
      tctaaaggt aaacataag tcaaaaata gaaaagtca 
      ccgcactta agcgagcct gaaatacaa ccgaacaat 
      gaaatttct gaaaatgta caggtgacg cctcagtca 
      ccctctatc gataagcga gttgataat gagaatgac 
      tctaaggtc gatgccgac gataaaaat attaatgag 
      agtgtccaa aggcaagaa aatgtcaac gacgttgat 
      aaagaacaa cttggtaat aaaaccgag tcacaagat 
      gacgaatct gacgatgag cctctaagc attattaaa 
      aaaaatgta tcagttgac aaacctgtt actgaaaat 
      aaacaaccg gaaacaaca aacgaacct tccgaacat 
      caagaacct gatcaagaa gtacaaact caaatcgac 
      gtaaaacaa gatcaggtc gaagaacaa gctccagaa 
      aaacctgaa gaaattgcc ataactgaa gtcgaaaca 
      gaaacaccc gaagacaaa ccttccgaa attgccata 
      actcacgaa gaatcttcg aaagaaaca gaagcagaa 
      aaagcagaa gacatatca gcttcgtta aatgaaaca 
      caagatgtt gaaatggca agtgtggaa gaaaagtca 
      gagcaaaca actgcaata agtgaagat aaacccgaa 
      atggaagaa cctcaagtt tctgaaagt attgctgac 
      tcagaaaat gttgaagtt tgtcagcaa cctcctcaa 
      aagacacca gaagtacct gttgaaagc actgaggtt 
      ttgaaagaa tcagcacgt attgagaag agtccgcgg 
      gataataag gatcgacat cggagtcca cggcgacaa 
      ctccgacga agtaacgac tacgcgaaa cctgactcc 
      aaagaggag cctgttaac agcggcccc atcaaacct 
      ttcaaaacc aaacacagt tgggagcgc agcttaagc 
      tcagcccct tctttgctt aaagtcgat cttgacagc 
      atacaaatc gtatgtcca accattgaa tttcccgaa 
      gaacccgac ctcagtttg gagctcgaa aaagaacgg 
      cggaagtct gtgaccgag acatctggc aagaaattg 
      gaacgcaaa gtatcggtc gagtacaag tcggacact 
      tctgaaaga ccgtcgttt gaaaaacag aattctgtt 
      aattcttct acgcaagac ggcgaagat aattcgaat 
      atcattgcg ttcaaccga aaaattagt atcgttgat 
      gacactgcg agcaaattg aaaccgcca ccaagtccg 
      gccaagaat ccagtttcg aatatactt tacattacg 
      aaccttgtc aggcctttc actgtgaaa cagttaaag 
      gagttgctg gaaaggacc gggaaagtg caagaggac 
      gggttctgg actgacagg attaagtcg aaatgttac 
      gtccagtat gagactcaa gaggaggct gaagcgacg 
      agaaacgcc ctccatgga gtccaatgg ccaattggc 
      aacggcaaa aaattaatc atcgatttt gcaacggct 
      gaagatatg gaaaatgcg aagaatcca ccagcgcca 
      cctccagcc ccagttcca gacacaaat gtttcaaca 
      aaagagaat ttggaacca gcggagagt aaacatgac 
      aaggcctcg aaggaagtg gagaagaag agacctgaa 
      ggaccggtt cgagagtgg gacatcggc aagaaggac 
      gtgcgtcga gaccgatca aggagcaga gaaagaagt 
      cgcaaacac aggcgtaca ccaagtcca tcagatgat 
      tttatcacc aggaagcag aggaaagcc gaggactcc 
      gtcccccaa aagttgatg gatgacctg ttcttgaaa 
      acgaaggcc actccgagc atctactgg cagcctttg 
      tcacccgaa gagatatcg atgaaacaa cagcagcgc 
      ctcatccgc atggaggaa cacaaaaga cgtatggaa 
      gagaattcg cgtatgaaa gggagagaa atggggagg 
      ggcgcgttc cgacgtaga tacgattaa
    mRNA sequence
    • >TC014271 RA
      atgcgactc ttcttgatt acaagcgag ttgtttaat 
      cgtgtgatt accgaattt ttgttagtt ttttgaata 
      aaaaatgga tgagaatta ataagtgtc ggctacggt 
      tacatctag gactattgc gagaagatg cggcgtaaa 
      agtgcccgt aacgcccag aaagcgtct cctgagaaa 
      gcgctcaaa gaaacggaa actcctccg aagacccgt 
      cgatcgcgg cgtcggaaa agatcacca tcgaaatcg 
      agcgaatct gaagatgaa gttctgaca ataacaaag 
      cgtgccccg cttgaagct acccccgat acaacaact 
      aagagtcac actccgagt gacgaggag acttgcgat 
      gaaccggtg tggaaagtg acctcttct gaggatact 
      cccaagggg gagatccag aagctaaag atttgtctg 
      acaaggcct tccccggat tcacctgat aaaagcaag 
      acacgtcgc agtaagtct aatagtcag actgaagac 
      gacgcgacg agttctgag gataaatcg tcaacaaga 
      cgaactcgc tcacgggga tctccactt gttagaaac 
      gacggtttg tctagtcaa gacggtgat gaagaagtg 
      gaagagaag aagggtaaa ggtaagtct aaaggtaaa 
      cataagtca aaaatagaa aagtcaccg cacttaagc 
      gagcctgaa atacaaccg aacaatgaa atttctgaa 
      aatgtacag gtgacgcct cagtcaccc tctatcgat 
      aagcgagtt gataatgag aatgactct aaggtcgat 
      gccgacgat aaaaatatt aatgagagt gtccaaagg 
      caagaaaat gtcaacgac gttgataaa gaacaactt 
      ggtaataaa accgagtca caagatgac gaatctgac 
      gatgagcct ctaagcatt attaaaaaa aatgtatca 
      gttgacaaa cctgttact gaaaataaa caaccggaa 
      acaacaaac gaaccttcc gaacatcaa gaacctgat 
      caagaagta caaactcaa atcgacgta aaacaagat 
      caggtcgaa gaacaagct ccagaaaaa cctgaagaa 
      attgccata actgaagtc gaaacagaa acacccgaa 
      gacaaacct tccgaaatt gccataact cacgaagaa 
      tcttcgaaa gaaacagaa gcagaaaaa gcagaagac 
      atatcagct tcgttaaat gaaacacaa gatgttgaa 
      atggcaagt gtggaagaa aagtcagag caaacaact 
      gcaataagt gaagataaa cccgaaatg gaagaacct 
      caagtttct gaaagtatt gctgactca gaaaatgtt 
      gaagtttgt cagcaacct cctcaaaag acaccagaa 
      gtacctgtt gaaagcact gaggttttg aaagaatca 
      gcacgtatt gagaagagt ccgcgggat aataaggat 
      cgacatcgg agtccacgg cgacaactc cgacgaagt 
      aacgactac gcgaaacct gactccaaa gaggagcct 
      gttaacagc ggccccatc aaacctttc aaaaccaaa 
      cacagttgg gagcgcagc ttaagctca gccccttct 
      ttgcttaaa gtcgatctt gacagcata caaatcgta 
      tgtccaacc attgaattt cccgaagaa cccgacctc 
      agtttggag ctcgaaaaa gaacggcgg aagtctgtg 
      accgagaca tctggcaag aaattggaa cgcaaagta 
      tcggtcgag tacaagtcg gacacttct gaaagaccg 
      tcgtttgaa aaacagaat tctgttaat tcttctacg 
      caagacggc gaagataat tcgaatatc attgcgttc 
      aaccgaaaa attagtatc gttgatgac actgcgagc 
      aaattgaaa ccgccacca agtccggcc aagaatcca 
      gtttcgaat atactttac attacgaac cttgtcagg 
      cctttcact gtgaaacag ttaaaggag ttgctggaa 
      aggaccggg aaagtgcaa gaggacggg ttctggact 
      gacaggatt aagtcgaaa tgttacgtc cagtatgag 
      actcaagag gaggctgaa gcgacgaga aacgccctc 
      catggagtc caatggcca attggcaac ggcaaaaaa 
      ttaatcatc gattttgca acggctgaa gatatggaa 
      aatgcgaag aatccacca gcgccacct ccagcccca 
      gttccagac acaaatgtt tcaacaaaa gagaatttg 
      gaaccagcg gagagtaaa catgacaag gcctcgaag 
      gaagtggag aagaagaga cctgaagga ccggttcga 
      gagtgggac atcggcaag aaggacgtg cgtcgagac 
      cgatcaagg agcagagaa agaagtcgc aaacacagg 
      cgtacacca agtccatca gatgatttt atcaccagg 
      aagcagagg aaagccgag gactccgtc ccccaaaag 
      ttgatggat gacctgttc ttgaaaacg aaggccact 
      ccgagcatc tactggcag cctttgtca cccgaagag 
      atatcgatg aaacaacag cagcgcctc atccgcatg 
      gaggaacac aaaagacgt atggaagag aattcgcgt 
      atgaaaggg agagaaatg gggaggggc gcgttccga 
      cgtagatac gattaatca ggaaagaaa gtctaaagt 
      taattttgt tgtgtgtgt gatgtgtgg cagttgtgc 
      gtgtgtaag ttttgtagc aaaaattat ttgattttg 
      aaccctacc ctactattt ctttaattt tgcctgagt 
      gaggacgga taaatgggg agataatcc tattttttg 
      taggatttt cagtatagg tttcacact ttcgttcag 
      cgagggatt ttcacagaa gcattaatt tacacgtta 
      gtggaaata gtagcattt cacacaaac taattgctg 
      ttaaaaagt attgtatga tttcgtgaa tgttttccg 
      atgaaacag ttctgtaaa act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY