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Gene information
General information
TC007257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6759900..6763712
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007257
      iBeetle screen iB_10737

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007257 RA
        MSKVVIYVL DKIVWMLLI TAVTTQALQ 
        IAGVVDAPK GLGDISYKQ DSEYNFQVQ 
        VGNSDVRGK RQTDQRSPL IDNIFSKQF 
        GQGEYFFRI NRTNVITAV M
      • >TC007257 RB
        MSKVVIYVL DKIVWMLLI TAVTTQALQ 
        IAGVVDAPK GLGDISYKQ DSEYNFQVQ 
        VGNSDVRGK RQTDQRSPL IDNIFSIPI 
        TTLNAVNNL VQNTRPAFQ SLREFAARR 
        FDRANKAAN KNQTRQQRP APVYVHSTE 
        ETVNTQ
      CDS sequence
      • >TC007257 RA
        atgagcaag gttgtgatt tacgttctg gacaagatc 
        gtctggatg ctgctgata acagcagtc accacacaa 
        gcgctccaa atcgctggg gttgtcgac gcccctaag 
        ggccttgga gacatttcg tacaaacag gacagcgaa 
        tacaatttt caagtccaa gttggcaac tccgacgtc 
        cgtggcaaa aggcaaacg gaccaaagg tctccatta 
        atcgacaac atattcagc aaacaattc gggcaaggt 
        gaatacttc ttcaggata aatcggacg aatgttatt 
        acggcggtt atgtag
      • >TC007257 RB
        atgagcaag gttgtgatt tacgttctg gacaagatc 
        gtctggatg ctgctgata acagcagtc accacacaa 
        gcgctccaa atcgctggg gttgtcgac gcccctaag 
        ggccttgga gacatttcg tacaaacag gacagcgaa 
        tacaatttt caagtccaa gttggcaac tccgacgtc 
        cgtggcaaa aggcaaacg gaccaaagg tctccatta 
        atcgacaac atattcagc attccgatt acaactctt 
        aatgccgtg aataatttg gtgcaaaat acaaggcct 
        gcatttcaa agtttgagg gaatttgct gcgaggagg 
        ttcgacaga gcaaataaa gccgctaat aaaaatcaa 
        acgagacag caaaggccg gcccccgtt tatgtgcat 
        tcgactgaa gaaactgta aatacacaa tga
      mRNA sequence
      • >TC007257 RA
        tccattgct ttggaacag ctggggctg accacacga 
        agaaatgtg aaagtaatc gagagaaag gtgcctgct 
        attcggtga aacagtgac atgaaggta gtgaagtct 
        acctgatcc agtgttggt ggtactgtt atattatct 
        ctagcgctg ccacagatt tcagtattt tgtacatag 
        tggctctga gaagcagat atccagtga tagagtgca 
        cagaacttg tattctaac acacgtttt aattataac 
        acaatgagc aaggttgtg atttacgtt ctggacaag 
        atcgtctgg atgctgctg ataacagca gtcaccaca 
        caagcgctc caaatcgct ggggttgtc gacgcccct 
        aagggcctt ggagacatt tcgtacaaa caggacagc 
        gaatacaat tttcaagtc caagttggc aactccgac 
        gtccgtggc aaaaggcaa acggaccaa aggtctcca 
        ttaatcgac aacatattc agcaaacaa ttcgggcaa 
        ggtgaatac ttcttcagg ataaatcgg acgaatgtt 
        attacggcg gttatgtag gactaagta agtctcaac 
        ttcatgctt catgatcca tttcaaaga gttacaatc 
        gcaaacatt ccgctttga gagtgtcgg tgggttttt 
        cagccgatt gtttcgttt tgcagattc cgattacaa 
        ctcttaatg ccgtgaata atttggtgc aaaatacaa 
        ggcctgcat ttcaaagtt tgagggaat ttgctgcga 
        ggaggttcg acagagcaa ataaagccg ctaataaaa 
        atcaaacga gacagcaaa ggccggccc ccgtttatg 
        tgcattcga ctgaagaaa ctgtaaata cacaatgat 
        ttgtaacca cgttgtaca atattttaa gccccattg 
        ttgattatt gtagagtag gttttattg tgttaaaat 
        ttcgtttcc attcttcat tgttgtatc atgaatgtg 
        tatttttaa gtgacgacc tgcgaagta tttattttt 
        aaatttgtc gcttgatta gtgattata cttttgtga 
        tattttatt accaccgac aaaaataaa tttattttt 
        atacg
      • >TC007257 RB
        tccattgct ttggaacag ctggggctg accacacga 
        agaaatgtg aaagtaatc gagagaaag gtgcctgct 
        attcggtga aacagtgac atgaaggta gtgaagtct 
        acctgatcc agtgttggt ggtactgtt atattatct 
        ctagcgctg ccacagatt tcagtattt tgtacatag 
        tggctctga gaagcagat atccagtga tagagtgca 
        cagaacttg tattctaac acacgtttt aattataac 
        acaatgagc aaggttgtg atttacgtt ctggacaag 
        atcgtctgg atgctgctg ataacagca gtcaccaca 
        caagcgctc caaatcgct ggggttgtc gacgcccct 
        aagggcctt ggagacatt tcgtacaaa caggacagc 
        gaatacaat tttcaagtc caagttggc aactccgac 
        gtccgtggc aaaaggcaa acggaccaa aggtctcca 
        ttaatcgac aacatattc agcattccg attacaact 
        cttaatgcc gtgaataat ttggtgcaa aatacaagg 
        cctgcattt caaagtttg agggaattt gctgcgagg 
        aggttcgac agagcaaat aaagccgct aataaaaat 
        caaacgaga cagcaaagg ccggccccc gtttatgtg 
        cattcgact gaagaaact gtaaataca caatgattt 
        gtaaccacg ttgtacaat attttaagc cccattgtt 
        gattattgt agagtaggt tttattgtg ttaaaattt 
        cgtttccat tcttcattg ttgtatcat gaatgtgta 
        tttttaagt gacgacctg cgaagtatt tatttttaa 
        atttgtcgc ttgattagt gattatact tttgtgata 
        ttttattac caccgacaa aaataaatt tatttttat 
        acg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY