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Gene information
General information
TC014586 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12133768..12135321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014586
    iBeetle screen iB_10736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014586 RA
      MFTRQATKV LRSLRKDKT CVRIEKRNH PITRNSSTN 
      VVIIGGGAM GASAAYWLT HKSPGTCTV TVVEKDPTY 
      ANCSTSRSV GGIRQQFSI PENVQMSLF GAKFIQNLK 
      NQFGPEADI AYAPNGYLI LASPQGAPQ LAQNFKLQQ 
      ELGATNTLL SKDQLREKF KWLNVDDIE LGSMGCEKE 
      GWFDPWGLL SLFKSAAVS KGAQFVHGE VQNFVFTND 
      NSLKGVKVR LPNRDEKIL EFDACVLAA GAQSGAIAR 
      MAGIGTGAG DLSVALPVE PRKRYVYRF DSQGKNVPP 
      VNSPLMIDY TGVYFRKDG PVFIGGRSP DSDEEPQAD 
      NLEVDFSYF DNTVWPLLA ARIPAFESV KVQSAWGGF 
      YEYNTFDAN GFIGPHCTY DNLFFATGF SGHGIQQSP 
      AVGLAIAEL ILDGRFKTI DLKRMGFER LMKQQPLYE 
      IGIY
    CDS sequence
    • >TC014586 RA
      atgttcacg cggcaagcc accaaagta ttacgcagt 
      ctgcgcaaa gataaaact tgcgtaaga atcgaaaaa 
      cggaatcat ccgatcact cgcaactct tcaaccaac 
      gttgtgata atcggcggg ggcgcaatg ggggcctct 
      gcggcctac tggctcacc cacaaatca ccaggtact 
      tgcaccgtc accgtcgtc gaaaaagac ccaacgtac 
      gccaattgc tcaacaagc cgctcagtt ggaggaata 
      agacagcaa ttttcaatc cccgaaaac gtacaaatg 
      tccctattc ggcgcaaaa ttcatccaa aacctcaaa 
      aaccaattc ggacccgaa gccgatatc gcgtacgcg 
      cccaacggc taccttatc ctagccagc ccccaaggc 
      gccccacaa ctcgcccaa aacttcaaa ctgcagcaa 
      gaactaggc gctactaac acactcctg agtaaagac 
      cagctccgg gagaagttc aagtggctg aacgttgac 
      gatatcgag ttggggtcc atgggttgt gagaaagag 
      ggctggttt gatccctgg gggctgttg tccttattt 
      aagagtgcc gctgttagt aaaggggca cagtttgtt 
      catggagaa gtccaaaat tttgttttt acgaacgat 
      aatagcttg aaaggtgta aaagtccgg ttaccgaac 
      cgggacgaa aaaatattg gagtttgat gcgtgcgtg 
      ttggcggcg ggggcgcag tccggggct attgcccgg 
      atggcgggg atcggcacg ggggcgggg gacttatca 
      gtagcgctt cccgtcgaa cctaggaaa cgctacgtt 
      tatcgcttt gatagtcaa ggtaaaaat gttcctccg 
      gtgaactcg cccctaatg atcgactac acgggggtg 
      tattttcgc aaagacggg cctgtgttc atcgggggg 
      cgctcgcct gactcggac gaggagccc caagccgat 
      aacttagaa gttgatttt agctacttt gacaatacg 
      gtgtggcca cttttagcc gcccgtatt ccggctttt 
      gaaagtgtc aaagtgcaa agtgcttgg ggcggcttt 
      tacgaatac aatactttt gatgctaat ggttttatt 
      gggccacac tgtacttac gataattta ttttttgcg 
      actggattt agcggacac gggattcaa caatcgcca 
      gctgttggt ttagcgatc gctgaattg attctggat 
      ggacggttc aagacaatt gacttgaaa cgaatgggt 
      tttgagcgg ttaatgaaa caacaacct ctgtatgaa 
      attggaatt tattaa
    mRNA sequence
    • >TC014586 RA
      tgtgtagaa aaatgttca cgcggcaag ccaccaaag 
      tattacgca gtctgcgca aagataaaa cttgcgtaa 
      gaatcgaaa aacggaatc atccgatca ctcgcaact 
      cttcaacca acgttgtga taatcggcg ggggcgcaa 
      tgggggcct ctgcggcct actggctca cccacaaat 
      caccaggta cttgcaccg tcaccgtcg tcgaaaaag 
      acccaacgt acgccaatt gctcaacaa gccgctcag 
      ttggaggaa taagacagc aattttcaa tccccgaaa 
      acgtacaaa tgtccctat tcggcgcaa aattcatcc 
      aaaacctca aaaaccaat tcggacccg aagccgata 
      tcgcgtacg cgcccaacg gctacctta tcctagcca 
      gcccccaag gcgccccac aactcgccc aaaacttca 
      aactgcagc aagaactag gcgctacta acacactcc 
      tgagtaaag accagctcc gggagaagt tcaagtggc 
      tgaacgttg acgatatcg agttggggt ccatgggtt 
      gtgagaaag agggctggt ttgatccct gggggctgt 
      tgtccttat ttaagagtg ccgctgtta gtaaagggg 
      cacagtttg ttcatggag aagtccaaa attttgttt 
      ttacgaacg ataatagct tgaaaggtg taaaagtcc 
      ggttaccga accgggacg aaaaaatat tggagtttg 
      atgcgtgcg tgttggcgg cgggggcgc agtccgggg 
      ctattgccc ggatggcgg ggatcggca cgggggcgg 
      gggacttat cagtagcgc ttcccgtcg aacctagga 
      aacgctacg tttatcgct ttgatagtc aaggtaaaa 
      atgttcctc cggtgaact cgcccctaa tgatcgact 
      acacggggg tgtattttc gcaaagacg ggcctgtgt 
      tcatcgggg ggcgctcgc ctgactcgg acgaggagc 
      cccaagccg ataacttag aagttgatt ttagctact 
      ttgacaata cggtgtggc cacttttag ccgcccgta 
      ttccggctt ttgaaagtg tcaaagtgc aaagtgctt 
      ggggcggct tttacgaat acaatactt ttgatgcta 
      atggtttta ttgggccac actgtactt acgataatt 
      tattttttg cgactggat ttagcggac acgggattc 
      aacaatcgc cagctgttg gtttagcga tcgctgaat 
      tgattctgg atggacggt tcaagacaa ttgacttga 
      aacgaatgg gttttgagc ggttaatga aacaacaac 
      ctctgtatg aaattggaa tttattaag tttacgaat 
      tttataaaa tgaattcca tgtgtataa taatgaatg 
      aacaatttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY