Status
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Gene information
General information
TC007566 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2940977..2946122
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007566
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007566 RA
      MHRRAATFS NVVTSCGHP SGPYPRRLE KVKFGVPLE 
      EVCKNDIPG PLLVLILKL NKEAPYRKD VFRAPGHQG 
      AMKKLIHFL QTGRLVNMD NFSVYTIAS VLKKFLRKI 
      PGGVFGRDI EQRFFQVLD KSDDREQRM EIETIITSL 
      PVYTQRLLV LLFGTFRVI ACNSEKAGT GMTSEALGV 
      SVAPSFFHS CVSDGKAAR MEDVKKFKV ASRVVKLLI 
      EQFSTSYLF GKDNYEFYS RVTGRVLRV QGEWICSFQ 
      YPPPTTKGS VQQQIYANE CGTLERYLL GHLSLGTET 
      WLLCESDRW HANYGLEAM AQVEECQST PALLESSIQ 
      PQVSSTSLG MIAEHSLLE SCTRLSISL EQNGLFQGN 
      NTSHSSSAT HSSRNSQNY SGPKMTLEE LRAVNRYAE 
      STRSLSYLP QVHERQTER MKTRSQWFL APSVECSSC 
      GGSLDLPLN EADVSALTN ALLRRSSSG TIVGIAGLS 
      LSAESVQKR PSIRRSNSR DKRHYLHRS ASRRNKENG 
      SRSNSFKGR SDRKGSNSR SSSFKIKCE NISRSCSFK 
      TKTELCTCP GGDITDDSN AGTPTAKKN ETICVTLTY 
      KPRI
    CDS sequence
    • >TC007566 RA
      atgcacagg cgagctgcc acattcagc aacgtggtc 
      acgtcctgt ggccaccct tccgggccc taccctcgg 
      aggctggag aaagtcaag ttcggggta cctctcgaa 
      gaagtctgc aaaaacgac attccggga ccacttctt 
      gtgttgatt ttaaaattg aataaggag gccccttat 
      cgcaaagac gtatttagg gcccctgga catcaaggt 
      gcaatgaag aaactgatc cactttctt cagactggt 
      agactagtt aatatggat aatttcagt gtgtataca 
      attgctagt gttttaaaa aaatttctt cggaagata 
      ccaggagga gtattcggc agggacata gaacaacgg 
      tttttccaa gttcttgat aaatcagac gatagagaa 
      caacgaatg gaaattgaa accataata acttcactg 
      ccagtttac acgcaaaga ttgttagtt ttgctcttc 
      ggtacgttt agagtgatt gcgtgtaac agtgagaaa 
      gctggcact gggatgacg agtgaagct cttggtgtt 
      agcgttgct ccaagtttt tttcattcc tgcgtaagc 
      gacggaaaa gcagcgagg atggaagat gttaagaaa 
      tttaaggta gcttcacgt gtcgtaaag ctccttata 
      gaacaattt tccacgtcg tatcttttt ggtaaagat 
      aattacgaa ttttattct cgagtgacc gggcgagtt 
      ttgcgagtc caaggcgaa tggatctgt tcatttcaa 
      tatccacca ccaacaacg aaaggttcc gttcaacag 
      caaatttat gcgaatgag tgcgggact ttggagcgc 
      tatcttctc ggtcatttg tctttggga acagaaaca 
      tggcttctc tgtgaaagt gaccgctgg cacgcaaat 
      tatggttta gaagcaatg gcacaagta gaagaatgc 
      cagtcaact ccagcttta ctggaatcg agcattcaa 
      cctcaagtt agttccact tctttagga atgatcgca 
      gagcacagt cttttagag tcttgtaca cgtctgtcg 
      atatctttg gaacaaaat ggattattt cagggtaat 
      aacacatca cactcatct agcgctact cactcatca 
      cgtaacagt cagaattat tccggacca aagatgaca 
      cttgaggaa ctcagagct gtcaataga tacgcagaa 
      agcacaaga agcttgagt taccttcct caggttcac 
      gaacgacag actgaacga atgaaaacg cgttctcag 
      tggttctta gcgccatct gttgaatgc tctagttgt 
      ggagggtct cttgacctc ccactgaac gaagcagac 
      gtttcggct ttaaccaat gcccttcta aggcgttcc 
      tcatcaggc actatagtc ggcatagct ggtctctcc 
      ctgagtgcc gagtccgtt cagaaaagg ccgagtatt 
      cgacggtca aactctcgt gataaacga cactatctc 
      catcgatca gctagtcga agaaacaaa gagaatggt 
      tccaggtcc aatagcttt aagggtcgc agtgatagg 
      aaaggctct aattcccgt tcaagtagt ttcaagatt 
      aagtgcgaa aacatatca agatcgtgc agtttcaag 
      accaagact gagctgtgc acttgtcca ggcggtgat 
      attacagat gacagtaat gctggtact cctactgcc 
      aaaaaaaat gaaacaatc tgtgtaact ttaacgtat 
      aagcctcgt atttaa
    mRNA sequence
    • >TC007566 RA
      tgtggtgta gaaattgga ggtgtggtt ggtgtttgt 
      aaaaaaatt tcaaacttc cgatgtgct caaaatgtg 
      taaaataat ccctagtgt tcgttacag tgcagttgt 
      tttgtttaa gtgaacgtt ttcagtttg aagatgttt 
      tgatgtgcg cttttagtt aggttatgc ttggatgag 
      cgtttctgg tcgttgtaa ttggaatta cacacgtta 
      gacggggct gttgagatg agcagttgg gcagcaagg 
      atgcacagg cgagctgcc acattcagc aacgtggtc 
      acgtcctgt ggccaccct tccgggccc taccctcgg 
      aggctggag aaagtcaag ttcggggta cctctcgaa 
      gaagtctgc aaaaacgac attccggga ccacttctt 
      gtgttgatt ttaaaattg aataaggag gccccttat 
      cgcaaagac gtatttagg gcccctgga catcaaggt 
      gcaatgaag aaactgatc cactttctt cagactggt 
      agactagtt aatatggat aatttcagt gtgtataca 
      attgctagt gttttaaaa aaatttctt cggaagata 
      ccaggagga gtattcggc agggacata gaacaacgg 
      tttttccaa gttcttgat aaatcagac gatagagaa 
      caacgaatg gaaattgaa accataata acttcactg 
      ccagtttac acgcaaaga ttgttagtt ttgctcttc 
      ggtacgttt agagtgatt gcgtgtaac agtgagaaa 
      gctggcact gggatgacg agtgaagct cttggtgtt 
      agcgttgct ccaagtttt tttcattcc tgcgtaagc 
      gacggaaaa gcagcgagg atggaagat gttaagaaa 
      tttaaggta gcttcacgt gtcgtaaag ctccttata 
      gaacaattt tccacgtcg tatcttttt ggtaaagat 
      aattacgaa ttttattct cgagtgacc gggcgagtt 
      ttgcgagtc caaggcgaa tggatctgt tcatttcaa 
      tatccacca ccaacaacg aaaggttcc gttcaacag 
      caaatttat gcgaatgag tgcgggact ttggagcgc 
      tatcttctc ggtcatttg tctttggga acagaaaca 
      tggcttctc tgtgaaagt gaccgctgg cacgcaaat 
      tatggttta gaagcaatg gcacaagta gaagaatgc 
      cagtcaact ccagcttta ctggaatcg agcattcaa 
      cctcaagtt agttccact tctttagga atgatcgca 
      gagcacagt cttttagag tcttgtaca cgtctgtcg 
      atatctttg gaacaaaat ggattattt cagggtaat 
      aacacatca cactcatct agcgctact cactcatca 
      cgtaacagt cagaattat tccggacca aagatgaca 
      cttgaggaa ctcagagct gtcaataga tacgcagaa 
      agcacaaga agcttgagt taccttcct caggttcac 
      gaacgacag actgaacga atgaaaacg cgttctcag 
      tggttctta gcgccatct gttgaatgc tctagttgt 
      ggagggtct cttgacctc ccactgaac gaagcagac 
      gtttcggct ttaaccaat gcccttcta aggcgttcc 
      tcatcaggc actatagtc ggcatagct ggtctctcc 
      ctgagtgcc gagtccgtt cagaaaagg ccgagtatt 
      cgacggtca aactctcgt gataaacga cactatctc 
      catcgatca gctagtcga agaaacaaa gagaatggt 
      tccaggtcc aatagcttt aagggtcgc agtgatagg 
      aaaggctct aattcccgt tcaagtagt ttcaagatt 
      aagtgcgaa aacatatca agatcgtgc agtttcaag 
      accaagact gagctgtgc acttgtcca ggcggtgat 
      attacagat gacagtaat gctggtact cctactgcc 
      aaaaaaaat gaaacaatc tgtgtaact ttaacgtat 
      aagcctcgt atttaaatc ggcttcaga aatccgaat 
      aggaaattc aataaaatc gtacgctta ttaaaatta 
      atattatag aaatgtttt acaaactat aggaaatgt 
      gttacaatt gacgattta tactggttt aaagtggtt 
      tcagattta aaggttttt cgggcacca cttgcagtt 
      aaaatccga attgggatt ttaaggcat tcatatttc 
      atgatttct gcaataatg ttgcttgca ttacctgcc 
      atatttttt gtattatct attattaca tagccgcaa 
      acggacggc agctattct aattactgt gacgtcaaa 
      atcactaat gagcaaaaa tgtggcagt taaatgcaa 
      cttttcaca tatcagttt agaatttac tgtaaaatt 
      tgtttctga ttgctcatt attacgcat catttaaat 
      ttaaaatca cgcgaacca gttattaat ttaaactaa 
      ttgtgacgt ggaattcgt taaagtgta agtagaaac 
      attcgagcc ttacgactg aattatgac gcgactgtt 
      gcatattaa catcactat gttacagct acgttaatt 
      caatattaa agtacaaaa gatgttgag tcttaagta 
      ttattttgt gtgttatgt ttgtttaat gttacggta 
      aaataattg aaaggtaca ttccaattt gtacatttt 
      gcctaagta tttggtgtt atttattac ttttttatt 
      tgtgtttgg atattattt ctgtgtact gctttgggt 
      attttgcgc actattcag tgttgatat tgaagaaga 
      gaattttac tatttgttg cttactata agtgttagt 
      acccaatta ttataaaca ccaaacaga gtactggct 
      ctctaaaat agttacttt ggtaaaact tgaaatgtc 
      ttggaagta caacgttca tttaagtat ttttataca 
      cattaatac tgattttat acatgtttt atatctctt 
      tcgatttct acataatct tatcgaatt gatatcttt 
      ttgttacat tccttgtca tgtctgttc agtttgtaa 
      taaagccag tgtaaagtg catcatttc ggtaagttc 
      cagaaatcg gaagttaac agagaaaac attaggtaa 
      agtataaaa gattcggta cctgcacat ttattctgt 
      taaaatacc aaagattag aatgatgca ttgtaatta 
      tctgcattt attttcaat tcaccaatt gccaaatga 
      ataccaaat tttatatgt aacgatctc cctttatca 
      gattttgtg ttcgggaag ttgcacgct gcacttcgt 
      taatgtttt atgtatttt ttttatatt aaataaata 
      gcatgaatt ttt
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