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Gene information
General information
TC009218 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3040358..3042865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009218
      iBeetle screen iB_10732

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009218 RA
        MDKQIRPVF TLELNHKIV PGLVTIGKY DGTHPCLTA 
        ATTADKVLI HSPHRKNPN TVGRVVWSE SNREIATLN 
        INQTITALT AGVLVPEDD KDVLIVGSE THILVYHVH 
        DNKDVFYKE CHDGVKAIT LGGFRDGKM PIVMVGGNS 
        SVHGFDHQG NEVFWTAVG DVVTSLILM DFDKDGSNE 
        LVVSSEDFK IRVFKGDQI INEQVETEV VTGLAPLPE 
        NRFAYSVSN GTVGVYDQD VRLWRVKSK HFAISMQSY 
        DLLGQGSTQ LITGWSNGK IDCRSIKTG EVLFKDTMN 
        SGVAGVVEG DYRSVGKTD AICASSEGE VRGYTTTKS 
        LSTPPGAGL EQDTVRELL ARKQALSLE LKHYENNTK 
        YNENAYNQM DSYESNGVI PANTRLQIG IATSDDKSK 
        HNHVEVYVS TNNTTIIKA VLIFAEGIF KGETHVVHP 
        PQNKLSSDI LIPLYIPRD TPVDIHIKA LVGYPNSVQ 
        FHVFEITRQ LPRFSMYAL LTASPPYDS FVEFKINDR 
        LQRICMWLN QNFLFPNDI EADGTSLKL HLKCLRDAS 
        VLTLSFDAS GKTLFFTEN MALAADLVQ SLATFLKIE 
        SLESKVSFP KQEDTLKIC MEKLGEIQD ARLRLGTDV 
        ADRIGLIRG LVIRAEDCR INDIDEMAK YYVELDRVN 
        KELISSYNI RLQNYNEGL ETMKNINSI IQRASRLRV 
        GPNSSTMIN HCRAAIKNN NIEGLLKII RTGEL
      CDS sequence
      • >TC009218 RA
        atggacaag caaatccgt cccgttttc accctcgaa 
        ctcaaccac aaaatcgtg ccaggcctt gtgaccatt 
        ggtaaatac gacggcacc cacccatgt ctcactgct 
        gcgaccacc gccgacaag gttctgatc cacagcccc 
        catcgcaaa aaccccaac accgtcgga cgagtggtt 
        tggtcggaa tccaaccgc gaaatcgcc actttgaac 
        ataaaccag accataacg gccctaacc gctggggtt 
        ctggtaccg gaagatgac aaagacgtg ctcatagtg 
        ggcagtgag acccatatt ctggtctat cacgtgcat 
        gataataag gacgttttt tacaaagag tgccatgat 
        ggggtcaaa gctatcact ttagggggt ttcagggat 
        gggaaaatg cccattgtg atggttggg ggtaattct 
        tcggtacat gggtttgac catcaaggg aatgaggtg 
        ttttggacc gctgtgggc gatgtggtc acatcgtta 
        atcctaatg gactttgat aaagacgga agcaatgaa 
        ttggtggta agctccgaa gacttcaaa attcgggtc 
        ttcaaaggc gaccaaata atcaacgaa caagtggaa 
        acggaagtg gtgaccggt ttggcacca cttccggaa 
        aacaggttc gcctattcg gtctcaaac ggaactgtg 
        ggtgtttac gaccaggat gtgcggctt tggcgcgtc 
        aagtccaaa catttcgca atttcgatg caaagctac 
        gatttgttg gggcaaggc tccactcaa ttaatcacg 
        ggttggtcg aacggtaaa atcgactgt cgctcgatt 
        aaaaccggg gaagtactg ttcaaagac accatgaat 
        tctggtgtt gcgggggtg gtggagggg gattaccgg 
        tcggttggc aaaactgac gcgatttgt gcgtcaagt 
        gaaggcgaa gttcgaggt tacaccacc actaaatca 
        ttgtccacg ccccctggg gctgggctg gagcaagac 
        acagtgaga gagttacta gcacgcaaa caggcactc 
        agtttggag ctaaaacat tacgaaaat aacactaaa 
        tataacgaa aatgcctac aatcaaatg gatagttac 
        gaaagtaat ggggtgatt cccgcaaac acgagacta 
        caaatcggg attgccacg agtgacgac aagtcgaaa 
        cataatcac gttgaagtt tacgtttcc acgaacaat 
        acaacaata atcaaagcg gtgttaatt ttcgccgaa 
        gggattttc aagggggaa acacatgtt gtgcacccg 
        ccccagaac aagttaagt tctgatatt ttaatacct 
        ttgtacatc ccccgggat acgccagtc gatatccat 
        attaaagct ttagttggt tatccaaac agtgtccag 
        ttccacgtt ttcgaaatc acccgtcaa ctcccccgc 
        ttttccatg tacgctctt ctaacagcg tcacccccg 
        tatgacagt ttcgtcgaa tttaaaatc aatgaccgc 
        cttcaaaga atttgcatg tggcttaac cagaacttt 
        ctcttccct aatgacata gaggcggac gggacgagt 
        ctcaaattg cacttaaag tgtctcagg gacgcgtcc 
        gtgctcact ttgtcgttt gacgcctcg gggaaaacc 
        ctgttcttt acggaaaac atggcactt gctgccgat 
        ttggtacaa tcactggcc acttttctc aaaattgaa 
        agtctggag tcgaaagtt tcgtttccg aagcaggaa 
        gacacttta aagatttgt atggagaag ttgggggag 
        atacaggac gcgaggttg aggttaggg acggacgtg 
        gctgatcgg atcggcttg attcggggg ttggtcatc 
        cgggccgaa gattgccga atcaatgac atagacgaa 
        atggccaaa tattacgtg gagctcgac cgagtcaat 
        aaggaattg atcagtagt tacaacatt cgcttacag 
        aactacaac gagggtttg gagactatg aaaaatatc 
        aattcgatt attcaaagg gcgtcacgt ttgagggtt 
        gggcccaat tcgtccact atgatcaat cattgccgg 
        gccgctatc aagaataac aatattgaa ggtctttta 
        aagattata cgaactggt gaattgtaa
      mRNA sequence
      • >TC009218 RA
        tcgcccatc atggacaag caaatccgt cccgttttc 
        accctcgaa ctcaaccac aaaatcgtg ccaggcctt 
        gtgaccatt ggtaaatac gacggcacc cacccatgt 
        ctcactgct gcgaccacc gccgacaag gttctgatc 
        cacagcccc catcgcaaa aaccccaac accgtcgga 
        cgagtggtt tggtcggaa tccaaccgc gaaatcgcc 
        actttgaac ataaaccag accataacg gccctaacc 
        gctggggtt ctggtaccg gaagatgac aaagacgtg 
        ctcatagtg ggcagtgag acccatatt ctggtctat 
        cacgtgcat gataataag gacgttttt tacaaagag 
        tgccatgat ggggtcaaa gctatcact ttagggggt 
        ttcagggat gggaaaatg cccattgtg atggttggg 
        ggtaattct tcggtacat gggtttgac catcaaggg 
        aatgaggtg ttttggacc gctgtgggc gatgtggtc 
        acatcgtta atcctaatg gactttgat aaagacgga 
        agcaatgaa ttggtggta agctccgaa gacttcaaa 
        attcgggtc ttcaaaggc gaccaaata atcaacgaa 
        caagtggaa acggaagtg gtgaccggt ttggcacca 
        cttccggaa aacaggttc gcctattcg gtctcaaac 
        ggaactgtg ggtgtttac gaccaggat gtgcggctt 
        tggcgcgtc aagtccaaa catttcgca atttcgatg 
        caaagctac gatttgttg gggcaaggc tccactcaa 
        ttaatcacg ggttggtcg aacggtaaa atcgactgt 
        cgctcgatt aaaaccggg gaagtactg ttcaaagac 
        accatgaat tctggtgtt gcgggggtg gtggagggg 
        gattaccgg tcggttggc aaaactgac gcgatttgt 
        gcgtcaagt gaaggcgaa gttcgaggt tacaccacc 
        actaaatca ttgtccacg ccccctggg gctgggctg 
        gagcaagac acagtgaga gagttacta gcacgcaaa 
        caggcactc agtttggag ctaaaacat tacgaaaat 
        aacactaaa tataacgaa aatgcctac aatcaaatg 
        gatagttac gaaagtaat ggggtgatt cccgcaaac 
        acgagacta caaatcggg attgccacg agtgacgac 
        aagtcgaaa cataatcac gttgaagtt tacgtttcc 
        acgaacaat acaacaata atcaaagcg gtgttaatt 
        ttcgccgaa gggattttc aagggggaa acacatgtt 
        gtgcacccg ccccagaac aagttaagt tctgatatt 
        ttaatacct ttgtacatc ccccgggat acgccagtc 
        gatatccat attaaagct ttagttggt tatccaaac 
        agtgtccag ttccacgtt ttcgaaatc acccgtcaa 
        ctcccccgc ttttccatg tacgctctt ctaacagcg 
        tcacccccg tatgacagt ttcgtcgaa tttaaaatc 
        aatgaccgc cttcaaaga atttgcatg tggcttaac 
        cagaacttt ctcttccct aatgacata gaggcggac 
        gggacgagt ctcaaattg cacttaaag tgtctcagg 
        gacgcgtcc gtgctcact ttgtcgttt gacgcctcg 
        gggaaaacc ctgttcttt acggaaaac atggcactt 
        gctgccgat ttggtacaa tcactggcc acttttctc 
        aaaattgaa agtctggag tcgaaagtt tcgtttccg 
        aagcaggaa gacacttta aagatttgt atggagaag 
        ttgggggag atacaggac gcgaggttg aggttaggg 
        acggacgtg gctgatcgg atcggcttg attcggggg 
        ttggtcatc cgggccgaa gattgccga atcaatgac 
        atagacgaa atggccaaa tattacgtg gagctcgac 
        cgagtcaat aaggaattg atcagtagt tacaacatt 
        cgcttacag aactacaac gagggtttg gagactatg 
        aaaaatatc aattcgatt attcaaagg gcgtcacgt 
        ttgagggtt gggcccaat tcgtccact atgatcaat 
        cattgccgg gccgctatc aagaataac aatattgaa 
        ggtctttta aagattata cgaactggt gaattgtaa 
        ttgttttgt gcaaaaata aatttgaat ttggcgt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY