Status
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Gene information
General information
TC033553
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009505
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 176147..179500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033553
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10729

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033553 RA
        MNEEDGFDL SQKARSLTT KISSFKNCK EPGLILEHV 
        KEAIEDLVL CTISEAEDI LKHVAPVIR ETVQEIYAK 
        NNLLVESND EHVLDAIKS NIQVALHFI DICYQVLDH 
        VTKYNTINF KEISSLPTN IAQIISSTF GHCKYSQAT 
        YSAFYKCVS EIVDVFRKT QELHTKFLN FLDRHLTFD 
        FLNENDVSA YTEILDVIL EISKTLLGV NMKSMSDNW 
        KTFVVLAQR HTKNVSPAI QLLTGEIDN NLKIISELE 
        IGDSNKAIQ LFKITGFLV KVVLKLFEC EFDILECYD 
        EVLRFWLMI YSWHACKFS EEFFESYNT IATLCTESL 
        LNFILHYNV FLKKFSTVI EKEDVITKN SFGVLIFAT 
        ILIKELHFQ KILDEHEIV SLISLIFNL TKNCFEEYF 
        FDNSNFYEE LITSVAIAI LLFPNVYLE LEKLLLECV 
        LQEKIWTTL IATDVWCLV LRHSSEGTC RSVFRVLIN 
        QMLELEFYS STIIVHLKV FTQRIFKML PVDIQIEEL 
        VKYDPNNIS VWKIISTRN IPLSHRYYI EFILSDLDK 
        TSQMNKEYY SVNDFMFLM ETLDVVSYT NFHLLEINA 
        ERLVPMLMK LWSFDLSEV SPGNKSLSF FIQKLLKIT 
        TVVGEELTV ENLILILNH LKPAVNDFL VQLLICDFL 
        RSISGRNFN SSLKKVSLF SLIIDVFLT LVGSKNAVV 
        VQTALECFT EFSNKENYG CVIQKVSTS SEFVKKLVN 
        DYVQRSSVN KYDLKYFQR IRNNNNIFR HHGFCLDTF 
        EEPSCKKIK LESDLIIEC LKNDTEKLI KMQLNSTQM 
        TEIRRVEQK LQTLLK
      CDS sequence
      • >TC033553 RA
        atgaacgaa gaagacggt tttgattta tcccaaaaa 
        gcccgcagt ttaacaaca aagataagt tcttttaaa 
        aattgtaag gaaccagga ctcattctc gaacacgtt 
        aaagaagca attgaagat ttagttctt tgtacgatt 
        tcagaagca gaagatatc ttaaaacat gtggcacca 
        gttataaga gagacagta caagagatt tatgcaaaa 
        aacaatttg ctcgtagag tcaaatgat gagcatgtt 
        ctggacgca atcaagtct aatattcag gtggcgtta 
        cattttatt gatatttgc taccaagtt ctggaccac 
        gtaacgaag tataacact attaatttt aaagagatt 
        tcaagtctt cctacaaat atagctcaa ataataagt 
        tcaacattt ggtcattgc aaatacagt caggccaca 
        tatagtgct ttttacaag tgtgtaagc gaaattgtg 
        gacgtgttt aggaaaacc caggagcta cataccaaa 
        tttttaaat tttttagat cgtcatctg actttcgat 
        tttttaaac gaaaacgat gtttcagcg tacacagag 
        attttagac gtcattctc gaaatcagc aaaactcta 
        ctaggggtt aatatgaaa tcaatgtca gataattgg 
        aagactttt gtagttttg gcacaaaga cacacaaaa 
        aatgtatca cccgctatc caattactg actggggaa 
        attgataac aatttgaaa atcatttca gaattggaa 
        attggtgac tcgaataag gcaattcag cttttcaaa 
        atcactgga tttttggtt aaagtcgtt ttgaagttg 
        ttcgagtgc gaatttgat attttggaa tgttacgat 
        gaggttttg aggttttgg cttatgatt tacagttgg 
        catgcctgt aaattctca gaagaattt tttgagtct 
        tataacacg atagcaaca ttgtgcaca gaatcgttg 
        ctaaacttt atacttcat tataacgtt tttttaaag 
        aaatttagt acagttata gagaaagag gacgtaatc 
        actaaaaac agtttcggc gttcttata tttgcaaca 
        attttgatc aaagagcta cactttcag aaaatttta 
        gatgaacat gaaattgtt tcgctcatc agcttaata 
        ttcaatttg actaaaaat tgttttgag gagtacttt 
        ttcgataat tcgaatttt tacgaggaa ttaattaca 
        agtgttgca attgcaatt ttattgttt cctaatgtt 
        tatctagaa ttggaaaaa ctgctgctg gaatgtgtt 
        ctacaggaa aaaatttgg acaacttta attgcgaca 
        gatgtctgg tgtttagtt ttgagacat tcctctgaa 
        ggaacatgc cgttcagtt ttccgagtt ttaataaat 
        caaatgtta gaacttgaa ttttattct agtacgatt 
        atagttcat cttaaagtt tttactcaa cgcatcttt 
        aaaatgtta ccggtggac atccaaatc gaagagttg 
        gtcaaatat gatccgaat aatatttca gtctggaaa 
        atcataagt acaagaaat attccactg tctcataga 
        tattatatc gaatttatt ttgagtgat ttggacaag 
        acgtctcaa atgaataaa gagtattat tcggtcaat 
        gattttatg tttttgatg gaaacactt gatgttgtt 
        tcttataca aacttccat cttcttgag ataaatgca 
        gaaagattg gttccgatg ttaatgaaa ttgtggtct 
        tttgatttg tctgaggtt tcacctgga aataaatct 
        ctcagtttt tttatccaa aaattatta aaaataact 
        acagtagta ggtgaagaa ttaactgtg gaaaattta 
        attttgata ttaaaccat ttaaaaccg gcagtaaac 
        gatttcctt gttcaacta cttatctgt gattttcta 
        cggtcgatt tctggtcga aattttaat agctccctt 
        aaaaaagtc tcacttttt tcgttaata atagacgta 
        tttctcaca cttgttgga agtaaaaat gctgtcgtt 
        gttcaaaca gcgttggag tgttttact gaattctct 
        aataaggaa aattacgga tgtgtgata caaaaggtg 
        tccacaagt tcagaattt gtgaaaaaa ttggtcaat 
        gattatgtg caaagaagt tctgtgaat aagtatgat 
        ttgaagtac tttcaacgt attcgtaat aataataac 
        atatttcga caccatggt ttttgtctc gacaccttt 
        gaagaacca agttgtaaa aaaattaaa cttgaaagt 
        gaccttatt atcgaatgt ttgaaaaat gatacggaa 
        aagttgatc aaaatgcag ttaaattcg acgcaaatg 
        actgaaatt agaagggtt gaacaaaag ttgcaaacg 
        ttacttaaa tga
      mRNA sequence
      • >TC033553 RA
        tatgttttg attaccgcc aaagacaac aataatgaa 
        cgaagaaga cggttttga tttatccca aaaagcccg 
        cagtttaac aacaaagat aagttcttt taaaaattg 
        taaggaacc aggactcat tctcgaaca cgttaaaga 
        agcaattga agatttagt tctttgtac gatttcaga 
        agcagaaga tatcttaaa acatgtggc accagttat 
        aagagagac agtacaaga gatttatgc aaaaaacaa 
        tttgctcgt agagtcaaa tgatgagca tgttctgga 
        cgcaatcaa gtctaatat tcaggtggc gttacattt 
        tattgatat ttgctacca agttctgga ccacgtaac 
        gaagtataa cactattaa ttttaaaga gatttcaag 
        tcttcctac aaatatagc tcaaataat aagttcaac 
        atttggtca ttgcaaata cagtcaggc cacatatag 
        tgcttttta caagtgtgt aagcgaaat tgtggacgt 
        gtttaggaa aacccagga gctacatac caaattttt 
        aaatttttt agatcgtca tctgacttt cgatttttt 
        aaacgaaaa cgatgtttc agcgtacac agagatttt 
        agacgtcat tctcgaaat cagcaaaac tctactagg 
        ggttaatat gaaatcaat gtcagataa ttggaagac 
        ttttgtagt tttggcaca aagacacac aaaaaatgt 
        atcacccgc tatccaatt actgactgg ggaaattga 
        taacaattt gaaaatcat ttcagaatt ggaaattgg 
        tgactcgaa taaggcaat tcagctttt caaaatcac 
        tggattttt ggttaaagt cgttttgaa gttgttcga 
        gtgcgaatt tgatatttt ggaatgtta cgatgaggt 
        tttgaggtt ttggcttat gatttacag ttggcatgc 
        ctgtaaatt ctcagaaga attttttga gtcttataa 
        cacgatagc aacattgtg cacagaatc gttgctaaa 
        ctttatact tcattataa cgttttttt aaagaaatt 
        tagtacagt tatagagaa agaggacgt aatcactaa 
        aaacagttt cggcgttct tatatttgc aacaatttt 
        gatcaaaga gctacactt tcagaaaat tttagatga 
        acatgaaat tgtttcgct catcagctt aatattcaa 
        tttgactaa aaattgttt tgaggagta ctttttcga 
        taattcgaa tttttacga ggaattaat tacaagtgt 
        tgcaattgc aattttatt gtttcctaa tgtttatct 
        agaattgga aaaactgct gctggaatg tgttctaca 
        ggaaaaaat ttggacaac tttaattgc gacagatgt 
        ctggtgttt agttttgag acattcctc tgaaggaac 
        atgccgttc agttttccg agttttaat aaatcaaat 
        gttagaact tgaatttta ttctagtac gattatagt 
        tcatcttaa agtttttac tcaacgcat ctttaaaat 
        gttaccggt ggacatcca aatcgaaga gttggtcaa 
        atatgatcc gaataatat ttcagtctg gaaaatcat 
        aagtacaag aaatattcc actgtctca tagatatta 
        tatcgaatt tattttgag tgatttgga caagacgtc 
        tcaaatgaa taaagagta ttattcggt caatgattt 
        tatgttttt gatggaaac acttgatgt tgtttctta 
        tacaaactt ccatcttct tgagataaa tgcagaaag 
        attggttcc gatgttaat gaaattgtg gtcttttga 
        tttgtctga ggtttcacc tggaaataa atctctcag 
        tttttttat ccaaaaatt attaaaaat aactacagt 
        agtaggtga agaattaac tgtggaaaa tttaatttt 
        gatattaaa ccatttaaa accggcagt aaacgattt 
        ccttgttca actacttat ctgtgattt tctacggtc 
        gatttctgg tcgaaattt taatagctc ccttaaaaa 
        agtctcact tttttcgtt aataataga cgtatttct 
        cacacttgt tggaagtaa aaatgctgt cgttgttca 
        aacagcgtt ggagtgttt tactgaatt ctctaataa 
        ggaaaatta cggatgtgt gatacaaaa ggtgtccac 
        aagttcaga atttgtgaa aaaattggt caatgatta 
        tgtgcaaag aagttctgt gaataagta tgatttgaa 
        gtactttca acgtattcg taataataa taacatatt 
        tcgacacca tggtttttg tctcgacac ctttgaaga 
        accaagttg taaaaaaat taaacttga aagtgacct 
        tattatcga atgtttgaa aaatgatac ggaaaagtt 
        gatcaaaat gcagttaaa ttcgacgca aatgactga 
        aattagaag ggttgaaca aaagttgca aacgttact 
        taaatgatt ttgtgttta tttctgtgc ttaataaag 
        tcttattac taca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY