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Gene information
General information
TC001460 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3881068..3883898
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001460
      iBeetle screen iB_10727

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001460 RA
        MENSLEDGK CNKDHIKDL GTSQTSLKS TKSLDKSST 
        SGSVKNEKI HFKEENHEH DNQKNTDAS LKVPHKFIA 
        NWRQACDRT KDKTKDLLK RWRTLPEIE TEKGKNDEN 
        VEKDEHSDS GWSVHVWTT WVDRFSIES EADNKENCQ 
        LTPTQNNKF SHFFSCLLD HDQDNLISE QDFETLIER 
        LRHFADWSI NSPEFNILR EVERGFIET FLCDISDEK 
        LGFTLNGEV YLTKDGWLH KWSQLTFGS KNLCDFPIW 
        LQFFVKVLF QVINRCGSG IITRDELSA FYSSVLGLD 
        TVKVGEILD IAYQAMTSN GDHPLIYKA YRLCFANYL 
        LGRYPNGPG QYILGAPPN ALSSAMFPV DYSALNTQP 
        EDLEQYAPD QKTNRRSVI V
      CDS sequence
      • >TC001460 RA
        atggagaac tcgctcgaa gacggcaaa tgtaacaaa 
        gaccacatc aaagactta ggtacgtct cagacaagt 
        ctcaaaagt acaaaaagc ctagataag agcagcact 
        agtggttcg gtcaaaaac gaaaaaata catttcaaa 
        gaggaaaac cacgaacat gacaatcaa aaaaacact 
        gatgcttcg ttaaaagtg cctcataaa tttatagcg 
        aattggaga caggcatgt gacagaact aaagacaag 
        acgaaagat ttgttaaag cgctggagg actttgccg 
        gagattgag accgaaaag gggaagaac gacgagaat 
        gtggagaag gacgagcac agcgacagc ggctggagc 
        gtccacgtc tggaccact tgggtcgac aggtttagc 
        atagagtcg gaagccgac aacaaggaa aattgccaa 
        ttaacacca acacagaac aataagttt tcacatttc 
        ttctcttgt ttgctcgac catgaccaa gacaattta 
        atcagtgag caagatttt gagacccta attgagaga 
        ttgcgacat tttgctgac tggtcgata aattctccg 
        gaatttaat attcttcga gaggtggag cgtggtttc 
        attgaaact tttttatgt gacatatct gacgaaaaa 
        ctgggcttc accctgaac ggggaagtg tatttaacg 
        aaagatgga tggttacat aaatggtca caattgact 
        tttggctca aaaaacctc tgtgacttt cccatatgg 
        ttgcagttt ttcgttaaa gttttgttt caagttatc 
        aaccggtgt gggtcggga attatcaca agggatgag 
        ctaagtgcg ttttattct tctgttctg ggcttagat 
        acagtgaaa gtgggggag attttagat atagcctat 
        caggccatg acttcgaac ggcgatcac ccgttgata 
        tacaaagca tatcgattg tgctttgcg aattatttg 
        ctggggagg tacccaaac ggaccagga caatatatt 
        ttgggtgct ccaccgaat gcgctctcg tcggcaatg 
        tttcctgtt gattactcg gcactgaat acacagccc 
        gaagacttg gaacaatat gcaccggat caaaagaca 
        aacagacgt agtgttatt gtatga
      mRNA sequence
      • >TC001460 RA
        atggtgcat aaaattagg ggctattat gtgtggtgt 
        gcgtaattt gaattcgta gcggaaagt gtgcacttt 
        acgatggag aactcgctc gaagacggc aaatgtaac 
        aaagaccac atcaaagac ttaggtacg tctcagaca 
        agtctcaaa agtacaaaa agcctagat aagagcagc 
        actagtggt tcggtcaaa aacgaaaaa atacatttc 
        aaagaggaa aaccacgaa catgacaat caaaaaaac 
        actgatgct tcgttaaaa gtgcctcat aaatttata 
        gcgaattgg agacaggca tgtgacaga actaaagac 
        aagacgaaa gatttgtta aagcgctgg aggactttg 
        ccggagatt gagaccgaa aaggggaag aacgacgag 
        aatgtggag aaggacgag cacagcgac agcggctgg 
        agcgtccac gtctggacc acttgggtc gacaggttt 
        agcatagag tcggaagcc gacaacaag gaaaattgc 
        caattaaca ccaacacag aacaataag ttttcacat 
        ttcttctct tgtttgctc gaccatgac caagacaat 
        ttaatcagt gagcaagat tttgagacc ctaattgag 
        agattgcga cattttgct gactggtcg ataaattct 
        ccggaattt aatattctt cgagaggtg gagcgtggt 
        ttcattgaa actttttta tgtgacata tctgacgaa 
        aaactgggc ttcaccctg aacggggaa gtgtattta 
        acgaaagat ggatggtta cataaatgg tcacaattg 
        acttttggc tcaaaaaac ctctgtgac tttcccata 
        tggttgcag tttttcgtt aaagttttg tttcaagtt 
        atcaaccgg tgtgggtcg ggaattatc acaagggat 
        gagctaagt gcgttttat tcttctgtt ctgggctta 
        gatacagtg aaagtgggg gagatttta gatatagcc 
        tatcaggcc atgacttcg aacggcgat cacccgttg 
        atatacaaa gcatatcga ttgtgcttt gcgaattat 
        ttgctgggg aggtaccca aacggacca ggacaatat 
        attttgggt gctccaccg aatgcgctc tcgtcggca 
        atgtttcct gttgattac tcggcactg aatacacag 
        cccgaagac ttggaacaa tatgcaccg gatcaaaag 
        acaaacaga cgtagtgtt attgtatga ttaaacttt 
        tatagaata tcgcaatat tagtattgc cacggcgca 
        cataaatct agtcatttt acattttta caatttaat 
        cgtctatga taaagtagt gcaatgagt tagatacta 
        tttcatttc ggtgcttct taactagta caaagtttt 
        aaattgtga ttttgtaaa atgataaat aaatagcat 
        tttacacaa gtgcacctt ctcaaaata aaattctaa 
        agtctatgt gatatttaa agttataat tgataacaa 
        tagaaccac atttgttat acaggtgtc ctagaatgt 
        ggtcacatt acacgaata tcgagatct taacatcca 
        gttgtaaat aaaatggtt gcgctgttg gtaatatat 
        tgtatccta gagtacgct atagtttgt tattataac 
        acactgtat tcattctag gaccgtgtg taactttac 
        attcagttg catgttatc ataatcatt tgtgatcat 
        ttgactgtt ctctcaagg ccataatca aatctattg 
        ttaacgggt ctttctata ttttataca aggaattgc 
        aacctttat cttaagtta ctttgctgt tatagttta 
        cttggcaaa gagtgtgca tatcatgta aaaatgaat 
        caaaagggc acaacgttt tgacagaat aaatgcact 
        taggataa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY