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Gene information
General information
TC003481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5828264..5831788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003481
    iBeetle screen iB_10725

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003481 RA
      MSSSRNDFP LEALNIHGP TFLKHALTS CTDPLKAIE 
      EFQVTNGIL LPSLRPMLP LLDLHGVRR LDFHMSVVE 
      ELREKLIAH INEIGKQEG RERDRKLKD LLVKSFPVV 
      KVKALRPIV MAILKNTPQ IDDNYLKIL VRDRELYND 
      TDTEVKRQI WKDNQSLFG DEVSPLLSQ YISEKENVL 
      FDHTNISTQ FFGPSPKVR RQGEVVQKL AHMIGNSVK 
      LYDMVLQFL RTLFLRTRN VHYCTLRAE LLMALHDLE 
      VQDIISIDP CHKFTWCLD ACIREKNVD IKRSRELQG 
      FLDNVKRGQ EQVLGDLSM TLCDPYAIN FLATSAIKI 
      LHHLIYVEG MPRDNTILI LLLRMLALG LSAWVMIDS 
      QDFKEPKLD SQVVTKFLP ALMSLMVDD QVRQLSGKL 
      PPDEREAAI TVIEHSGPL PDAVEAYIQ DSSVASILA 
      MYYTLNVAR LKDRVGLLR VLTVLANCK DDRAFEDPF 
      LHSLVIFLI HNPEEFQAE DFCTVLFDE FFFAGLTRE 
      NVTRHLLKL LWYVHSRLP ASRLHTLMK ALQPTHQHS 
      EKVHNLYET LQTKINSQE ETAVPLEMD IDLNSPLMS 
      VPTPAPYHH TL
    CDS sequence
    • >TC003481 RA
      atgtcttca tccagaaac gatttccct ttggaagcc 
      ctcaatata cacgggccc accttcctc aaacatgcc 
      ttaactagt tgcactgat ccgctcaaa gccattgaa 
      gagtttcag gttacaaac gggatactt ttaccctca 
      ttacgccca atgcttcca ttacttgat ctacacggg 
      gtgagaaga ctggatttt cacatgtca gttgtagag 
      gaactccga gagaaacta atcgcacac atcaatgaa 
      atcggcaag caagaagga cgcgaaagg gacaggaag 
      ctgaaagat ttactcgta aagagtttc cctgtggtc 
      aaagtgaag gctttaagg ccgatagtg atggcgatt 
      ttgaaaaac acaccacag attgacgac aactatttg 
      aaaatatta gttagagat cgggaactg tacaacgac 
      actgacaca gaagtcaaa cggcagatc tggaaggac 
      aaccagtcg ctcttcgga gatgaggtc tcgcctctc 
      ttgagtcag tacatcagc gaaaaagag aacgtgctg 
      ttcgatcac accaacatc agcacccag ttcttcggg 
      ccttccccc aaggtgcgg cgccaaggg gaagtcgtc 
      cagaagttg gcacacatg attggaaac agtgttaaa 
      ttgtacgat atggtgctc cagtttctt cgcactttg 
      tttttgcgg acccgaaac gtgcattac tgcactttg 
      agggccgaa ttattaatg gccttacac gatttggaa 
      gttcaggat ataatttcg atagatcca tgccataaa 
      tttacgtgg tgccttgat gcctgcatt agggaaaaa 
      aatgtcgac attaaacgc tcaagggag ttgcaaggg 
      tttttggat aacgttaag aggggccaa gagcaggtg 
      ctgggcgat ttatcaatg actttatgc gacccctac 
      gccattaat tttttggcc acgtcggcc ataaaaatc 
      ctgcatcat ctcatatac gtagaaggg atgccgagg 
      gacaatacg attttaatt ttgctcttg cgaatgttg 
      gctttgggt ctaagtgcc tgggtcatg atcgactcc 
      caagacttc aaagagccc aaactggac agtcaggtc 
      gttacgaaa tttttgccg gccctaatg tcgctcatg 
      gtcgacgac caagtgcgg caattgtca ggcaaattg 
      cccccagat gaacgcgaa gctgctatc acagttatc 
      gaacattcg gggcccttg cccgatgca gtggaggcc 
      tacattcaa gacagttca gtcgccagt attctcgcc 
      atgtactac accctgaac gtggcccgg cttaaagac 
      agggtgggg ctgctgcga gtcttgacg gttttggcg 
      aactgtaaa gacgaccgg gcttttgag gacccgttc 
      ctccactct ctcgtcatt ttccttatt cataatccg 
      gaggagttt caagctgag gatttttgt actgttctc 
      ttcgacgaa ttctttttt gctgggctg acgagggag 
      aacgtgaca agacattta ctgaaattg ctgtggtat 
      gtacattcc cgactgccg gctagtcgt ttacacacg 
      ctgatgaaa gcgctgcaa ccgacgcac caacacagc 
      gaaaaggtt cacaatttg tacgaaacg cttcagact 
      aagattaat tcgcaagag gagactgct gtacctctt 
      gaaatggat attgacctc aactcgcct ctaatgagt 
      gtgccaacg cctgctccg tatcaccat acgttatga
    mRNA sequence
    • >TC003481 RA
      ttgttttgt ttgacgctt ttgacagca aatggtgtg 
      ttttttgac aatatattt tcataatgg gttatgaaa 
      gctgtgtgt ttatattta ttgagatgt gtaattgta 
      atctctgtg atactaaca ccgaattta agtgataaa 
      ctgcgtcga tttgatgtt ttactagtg taaggattc 
      gtactgcaa aatgtcttc atccagaaa cgatttccc 
      tttggaagc cctcaatat acacgggcc caccttcct 
      caaacatgc cttaactag ttgcactga tccgctcaa 
      agccattga agagtttca ggttacaaa cgggatact 
      tttaccctc attacgccc aatgcttcc attacttga 
      tctacacgg ggtgagaag actggattt tcacatgtc 
      agttgtaga ggaactccg agagaaact aatcgcaca 
      catcaatga aatcggcaa gcaagaagg acgcgaaag 
      ggacaggaa gctgaaaga tttactcgt aaagagttt 
      ccctgtggt caaagtgaa ggctttaag gccgatagt 
      gatggcgat tttgaaaaa cacaccaca gattgacga 
      caactattt gaaaatatt agttagaga tcgggaact 
      gtacaacga cactgacac agaagtcaa acggcagat 
      ctggaagga caaccagtc gctcttcgg agatgaggt 
      ctcgcctct cttgagtca gtacatcag cgaaaaaga 
      gaacgtgct gttcgatca caccaacat cagcaccca 
      gttcttcgg gccttcccc caaggtgcg gcgccaagg 
      ggaagtcgt ccagaagtt ggcacacat gattggaaa 
      cagtgttaa attgtacga tatggtgct ccagtttct 
      tcgcacttt gtttttgcg gacccgaaa cgtgcatta 
      ctgcacttt gagggccga attattaat ggccttaca 
      cgatttgga agttcagga tataatttc gatagatcc 
      atgccataa atttacgtg gtgccttga tgcctgcat 
      tagggaaaa aaatgtcga cattaaacg ctcaaggga 
      gttgcaagg gtttttgga taacgttaa gaggggcca 
      agagcaggt gctgggcga tttatcaat gactttatg 
      cgaccccta cgccattaa ttttttggc cacgtcggc 
      cataaaaat cctgcatca tctcatata cgtagaagg 
      gatgccgag ggacaatac gattttaat tttgctctt 
      gcgaatgtt ggctttggg tctaagtgc ctgggtcat 
      gatcgactc ccaagactt caaagagcc caaactgga 
      cagtcaggt cgttacgaa atttttgcc ggccctaat 
      gtcgctcat ggtcgacga ccaagtgcg gcaattgtc 
      aggcaaatt gcccccaga tgaacgcga agctgctat 
      cacagttat cgaacattc ggggccctt gcccgatgc 
      agtggaggc ctacattca agacagttc agtcgccag 
      tattctcgc catgtacta caccctgaa cgtggcccg 
      gcttaaaga cagggtggg gctgctgcg agtcttgac 
      ggttttggc gaactgtaa agacgaccg ggcttttga 
      ggacccgtt cctccactc tctcgtcat tttccttat 
      tcataatcc ggaggagtt tcaagctga ggatttttg 
      tactgttct cttcgacga attcttttt tgctgggct 
      gacgaggga gaacgtgac aagacattt actgaaatt 
      gctgtggta tgtacattc ccgactgcc ggctagtcg 
      tttacacac gctgatgaa agcgctgca accgacgca 
      ccaacacag cgaaaaggt tcacaattt gtacgaaac 
      gcttcagac taagattaa ttcgcaaga ggagactgc 
      tgtacctct tgaaatgga tattgacct caactcgcc 
      tctaatgag tgtgccaac gcctgctcc gtatcacca 
      tacgttatg aaaacagtg gtattttat aattttgta 
      ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY