Status
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Gene information
General information
TC033310
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015335
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 280091..281652
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033310
    iBeetle screen iB_10724

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033310 RA
      MGLFHGFLY CFLWYSSIM AGYAMFCPL LPLLFISNK 
      FYRTCTDAL FTFWQFYPT VLLELLCGC EIQVSGDAI 
      QTDETSLIV MNHRTRTDW NFLWPTMYH SISGSGKFK 
      FATKFVLKD VIRHIPGPG WVMQLACFV YIKRCWGLD 
      KLTLGKAID YFSELGYKY SLLVFPEGT DFTDSTKAK 
      SDNFARKNG LESYDFVLH PRTTGFVFL AKKMLEKKA 
      LDAVYDVTL VYPDLVPQN EAILLKGNF PKLVKVHLA 
      RYPSAVLPK TEAGLCDFL QKRWLDKER TLREYHSTT 
      QFLHGRILK SENRWELLL ALFFWTLLP FFVTYLLCV 
      YWWYRFVIL GHTVFLIIV NVTMGGFQN FEIWWYHLR 
      RKN
    • >TC033310 RB
      MAGYAMFCP LLPLLFISN KFYRTCTDA LFTFWQFYP 
      TVLLELLCG CEIQVSGDA IQTDETSLI VMNHRTRTD 
      WNFLWPTMY HSISGSGKF KFATKFVLK DVIRHIPGP 
      GWVMQLACF VYIKRCWGL DKLTLGKAI DYFSELGYK 
      YSLLVFPEG TDFTDSTKA KSDNFARKN GLESYDFVL 
      HPRTTGFVF LAKKMLEKK ALDAVYDVT LVYPDLVPQ 
      NEAILLKGN FPKLVKVHL ARYPSAVLP KTEAGLCDF 
      LQKRWLDKE RTLREYHST TQFLHGRIL KSENRWELL 
      LALFFWTLL PFFVTYLLC VYWWYRFVI LGHTVFLII 
      VNVTMGGFQ NFEIWWYHL RRKN
    CDS sequence
    • >TC033310 RA
      atgggcttg tttcacggt tttttgtat tgttttttg 
      tggtattcg agtataatg gctggctat gcgatgttt 
      tgtcccctt ttgccactt ctgtttatt tcaaacaag 
      ttttaccga acgtgtacg gacgcttta tttacgttc 
      tggcagttt tatccgacg gtgttgctc gagttactg 
      tgcggatgc gagatacaa gtatctggt gatgccatt 
      caaacggac gaaacgtcg ctcattgtt atgaatcat 
      cggacgcgg acggattgg aattttttg tggcccacg 
      atgtatcac tctatttca ggaagcggg aaattcaaa 
      tttgccacc aaatttgtg ctaaaagat gtcataaga 
      cacattcca gggccgggc tgggtcatg caattggcc 
      tgttttgtc tatattaag agatgttgg ggtttggat 
      aagttaact ctggggaaa gcgattgat tatttctca 
      gaacttgga tataagtat agtttgttg gtttttcct 
      gaaggtacc gactttacc gatagtacc aaagccaaa 
      agtgataac tttgcacga aaaaatggg cttgaaagt 
      tacgatttt gttctgcat cctagaact acaggtttt 
      gtgtttttg gccaaaaaa atgttagag aagaaggcg 
      ctggatgcg gtttatgac gtcacgttg gtctatcct 
      gatttggta ccacaaaac gaagctatt ttattgaaa 
      ggaaatttt ccaaaactg gtcaaagta catttagca 
      aggtaccct agtgccgtt ttgcccaaa acggaggcc 
      ggcctttgc gattttttg caaaaacgc tggttggac 
      aaagaacgt actctacgt gagtaccac tcaacgact 
      caatttctc cacggacga atcctcaaa agtgaaaat 
      cgttgggaa ctacttctt gcgctattt ttttggaca 
      ttgttgccg ttttttgtc acttattta ttgtgtgtg 
      tattggtgg taccgtttt gtgattttg ggccatacc 
      gtgttttta attattgta aatgtgaca atgggtggg 
      ttccaaaat ttcgaaatc tggtggtac caccttagg 
      agaaaaaac tag
    • >TC033310 RB
      atggctggc tatgcgatg ttttgtccc cttttgcca 
      cttctgttt atttcaaac aagttttac cgaacgtgt 
      acggacgct ttatttacg ttctggcag ttttatccg 
      acggtgttg ctcgagtta ctgtgcgga tgcgagata 
      caagtatct ggtgatgcc attcaaacg gacgaaacg 
      tcgctcatt gttatgaat catcggacg cggacggat 
      tggaatttt ttgtggccc acgatgtat cactctatt 
      tcaggaagc gggaaattc aaatttgcc accaaattt 
      gtgctaaaa gatgtcata agacacatt ccagggccg 
      ggctgggtc atgcaattg gcctgtttt gtctatatt 
      aagagatgt tggggtttg gataagtta actctgggg 
      aaagcgatt gattatttc tcagaactt ggatataag 
      tatagtttg ttggttttt cctgaaggt accgacttt 
      accgatagt accaaagcc aaaagtgat aactttgca 
      cgaaaaaat gggcttgaa agttacgat tttgttctg 
      catcctaga actacaggt tttgtgttt ttggccaaa 
      aaaatgtta gagaagaag gcgctggat gcggtttat 
      gacgtcacg ttggtctat cctgatttg gtaccacaa 
      aacgaagct attttattg aaaggaaat tttccaaaa 
      ctggtcaaa gtacattta gcaaggtac cctagtgcc 
      gttttgccc aaaacggag gccggcctt tgcgatttt 
      ttgcaaaaa cgctggttg gacaaagaa cgtactcta 
      cgtgagtac cactcaacg actcaattt ctccacgga 
      cgaatcctc aaaagtgaa aatcgttgg gaactactt 
      cttgcgcta tttttttgg acattgttg ccgtttttt 
      gtcacttat ttattgtgt gtgtattgg tggtaccgt 
      tttgtgatt ttgggccat accgtgttt ttaattatt 
      gtaaatgtg acaatgggt gggttccaa aatttcgaa 
      atctggtgg taccacctt aggagaaaa aactag
    mRNA sequence
    • >TC033310 RA
      aataaattc atctcaacc ctcaccatg taatctttc 
      gtaaaattc gccgtattt taatttttt ttcaccgaa 
      atgggcttg tttcacggt tttttgtat tgttttttg 
      tggtattcg agtataatg gctggctat gcgatgttt 
      tgtcccctt ttgccactt ctgtttatt tcaaacaag 
      ttttaccga acgtgtacg gacgcttta tttacgttc 
      tggcagttt tatccgacg gtgttgctc gagttactg 
      tgcggatgc gagatacaa gtatctggt gatgccatt 
      caaacggac gaaacgtcg ctcattgtt atgaatcat 
      cggacgcgg acggattgg aattttttg tggcccacg 
      atgtatcac tctatttca ggaagcggg aaattcaaa 
      tttgccacc aaatttgtg ctaaaagat gtcataaga 
      cacattcca gggccgggc tgggtcatg caattggcc 
      tgttttgtc tatattaag agatgttgg ggtttggat 
      aagttaact ctggggaaa gcgattgat tatttctca 
      gaacttgga tataagtat agtttgttg gtttttcct 
      gaaggtacc gactttacc gatagtacc aaagccaaa 
      agtgataac tttgcacga aaaaatggg cttgaaagt 
      tacgatttt gttctgcat cctagaact acaggtttt 
      gtgtttttg gccaaaaaa atgttagag aagaaggcg 
      ctggatgcg gtttatgac gtcacgttg gtctatcct 
      gatttggta ccacaaaac gaagctatt ttattgaaa 
      ggaaatttt ccaaaactg gtcaaagta catttagca 
      aggtaccct agtgccgtt ttgcccaaa acggaggcc 
      ggcctttgc gattttttg caaaaacgc tggttggac 
      aaagaacgt actctacgt gagtaccac tcaacgact 
      caatttctc cacggacga atcctcaaa agtgaaaat 
      cgttgggaa ctacttctt gcgctattt ttttggaca 
      ttgttgccg ttttttgtc acttattta ttgtgtgtg 
      tattggtgg taccgtttt gtgattttg ggccatacc 
      gtgttttta attattgta aatgtgaca atgggtggg 
      ttccaaaat ttcgaaatc tggtggtac caccttagg 
      agaaaaaac tagtttaag tacctaatt attattgga 
      aaatgtgaa ttcaaatgt gccaaaata tttatgtat 
      taattaata aagtcggtg tttgaag
    • >TC033310 RB
      tctcaaccc tcaccatgt aatctttcg taaaattcg 
      ccgtatttt aattttttt tcaccgaaa tgggcttgt 
      ttcacggtt ttttgtatt gttttttgt ggtattcga 
      gtataatgg ctggctatg cgatgtttt gtccccttt 
      tgccacttc tgtttattt caaacaagt tttaccgaa 
      cgtgtacgg acgctttat ttacgttct ggcagtttt 
      atccgacgg tgttgctcg agttactgt gcggatgcg 
      agatacaag tatctggtg atgccattc aaacggacg 
      aaacgtcgc tcattgtta tgaatcatc ggacgcgga 
      cggattgga attttttgt ggcccacga tgtatcact 
      ctatttcag gaagcggga aattcaaat ttgccacca 
      aatttgtgc taaaagatg tcataagac acattccag 
      ggccgggct gggtcatgc aattggcct gttttgtct 
      atattaaga gatgttggg gtttggata agttaactc 
      tggggaaag cgattgatt atttctcag aacttggat 
      ataagtata gtttgttgg tttttcctg aaggtaccg 
      actttaccg atagtacca aagccaaaa gtgataact 
      ttgcacgaa aaaatgggc ttgaaagtt acgattttg 
      ttctgcatc ctagaacta caggttttg tgtttttgg 
      ccaaaaaaa tgttagaga agaaggcgc tggatgcgg 
      tttatgacg tcacgttgg tctatcctg atttggtac 
      cacaaaacg aagctattt tattgaaag gaaattttc 
      caaaactgg tcaaagtac atttagcaa ggtacccta 
      gtgccgttt tgcccaaaa cggaggccg gcctttgcg 
      attttttgc aaaaacgct ggttggaca aagaacgta 
      ctctacgtg agtaccact caacgactc aatttctcc 
      acggacgaa tcctcaaaa gtgaaaatc gttgggaac 
      tacttcttg cgctatttt tttggacat tgttgccgt 
      tttttgtca cttatttat tgtgtgtgt attggtggt 
      accgttttg tgattttgg gccataccg tgtttttaa 
      ttattgtaa atgtgacaa tgggtgggt tccaaaatt 
      tcgaaatct ggtggtacc accttagga gaaaaaact 
      agtttaagt acctaatta ttattggaa aatgtgaat 
      tcaaatgtg ccaaaatat ttatgtatt aattaataa 
      agtcggtgt ttgaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY