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Gene information
General information
TC013337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10922448..10924054
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013337
    iBeetle screen iB_10723

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013337 RA
      MLNTFRARN NCEQEAKFV QDRIVSVER TIGELCHVF 
      GQYSRKAAR LRDKGDEIA KVTLEHAEA EQLNRSLSL 
      ALENFADCI SLLSSYGDL RVQNLDSKV IKEFARYED 
      ICKHAKDEV KQIYNAREK EIARKRQLD RIKERNPRN 
      RQQIIQAET ELVKASAEF SKTLRNLEE KSTEFERQK 
      LHDVKSILL DFVAIEMGY HARALEVLT NAYKAVNSM 
      DEEADLQDF QKTSGKVEA EFKKSLRRP DSSRNVGSR 
      SLFRSSNSL GSLKGLFTP NNKKPEGIP RKASKSEET 
      LDSMKRSIS DTEDSLSDS ANVSENVSD TEEPSSPIR 
      PRKPRK
    • >TC013337 RB
      MLNTFRARN NCEQEAKFV QDRIVSVER TIGELCHVF 
      GQYSRKAAR LRDKGDEIA KVTLEHAEA EQLNRSLSL 
      ALENFADCI SLLSSYGDL RVQNLDSKV IKEFARYED 
      ICKHAKDEV KQIYNAREK EIARKRQLD RIKERNPRN 
      RQQIIQAET ELVKASAEF SKTLRNLEE KSTEFERQK 
      LHDVKSILL DFVAIEMGY HARALEVLT NAYKAVNSM 
      DEEADLQEF KKSLRRPDS SRNVGSRSL FRSSNSLGS 
      LKGLFTPNN KKPEGIPRK ASKSEETLD SMKRSISDT 
      EDSLSDSAN VSENVSDTE EPSSPIRPR KPRK
    CDS sequence
    • >TC013337 RA
      atgttgaac acgttccgg gcgcggaat aattgcgaa 
      caggaggct aaattcgtc caagaccgt attgtctca 
      gtcgagcga acaatcggt gaattatgc cacgttttc 
      gggcagtat tcccggaaa gcggcccgt ctgagagac 
      aaaggggac gaaattgcc aaagtcacg ctcgaacac 
      gctgaagcg gaacaacta aaccgatct ctaagccta 
      gctttggag aattttgcc gactgtatc tcgcttttg 
      agttcttac ggcgacctc agagtccag aatttggac 
      agtaaagtg attaaagag tttgcacgc tatgaggac 
      atttgtaaa catgccaaa gacgaagtt aagcaaatt 
      tacaacgct agggagaaa gaaattgcg aggaaaaga 
      cagctggat cggattaaa gaacgcaat ccgaggaat 
      aggcaacaa attatccaa gctgagacc gagttagta 
      aaggcgagt gccgagttt tcgaagact ttgcgtaac 
      ttggaggaa aaaagcacg gagtttgag agacaaaaa 
      ctgcacgat gttaagtcg attttgctc gactttgtt 
      gcgatagaa atggggtac cacgcccgg gctctggag 
      gtcttgacc aatgcctac aaagctgtg aattcgatg 
      gacgaagag gccgactta caggacttt caaaaaact 
      agtggaaag gtggaggcg gagtttaaa aagtcgctg 
      cggcgcccg gacagttca cgcaacgtg ggttcgcgc 
      agtcttttt cgctctagc aattcgctg ggttccctc 
      aagggtctt tttaccccc aataacaaa aaaccagag 
      gggattccc agaaaagcg tccaaaagt gaggaaacg 
      ctcgactcc atgaaacgg agcatttcc gacactgag 
      gacagcctc agcgacagt gcgaacgtc tctgagaat 
      gtttccgac actgaggag ccctcgtcc cccatcaga 
      ccgcggaag ccgcgcaaa tga
    • >TC013337 RB
      atgttgaac acgttccgg gcgcggaat aattgcgaa 
      caggaggct aaattcgtc caagaccgt attgtctca 
      gtcgagcga acaatcggt gaattatgc cacgttttc 
      gggcagtat tcccggaaa gcggcccgt ctgagagac 
      aaaggggac gaaattgcc aaagtcacg ctcgaacac 
      gctgaagcg gaacaacta aaccgatct ctaagccta 
      gctttggag aattttgcc gactgtatc tcgcttttg 
      agttcttac ggcgacctc agagtccag aatttggac 
      agtaaagtg attaaagag tttgcacgc tatgaggac 
      atttgtaaa catgccaaa gacgaagtt aagcaaatt 
      tacaacgct agggagaaa gaaattgcg aggaaaaga 
      cagctggat cggattaaa gaacgcaat ccgaggaat 
      aggcaacaa attatccaa gctgagacc gagttagta 
      aaggcgagt gccgagttt tcgaagact ttgcgtaac 
      ttggaggaa aaaagcacg gagtttgag agacaaaaa 
      ctgcacgat gttaagtcg attttgctc gactttgtt 
      gcgatagaa atggggtac cacgcccgg gctctggag 
      gtcttgacc aatgcctac aaagctgtg aattcgatg 
      gacgaagag gccgactta caggagttt aaaaagtcg 
      ctgcggcgc ccggacagt tcacgcaac gtgggttcg 
      cgcagtctt tttcgctct agcaattcg ctgggttcc 
      ctcaagggt ctttttacc cccaataac aaaaaacca 
      gaggggatt cccagaaaa gcgtccaaa agtgaggaa 
      acgctcgac tccatgaaa cggagcatt tccgacact 
      gaggacagc ctcagcgac agtgcgaac gtctctgag 
      aatgtttcc gacactgag gagccctcg tcccccatc 
      agaccgcgg aagccgcgc aaatga
    mRNA sequence
    • >TC013337 RA
      gcaaataaa aacaattca acaataatg ttgaacacg 
      ttccgggcg cggaataat tgcgaacag gaggctaaa 
      ttcgtccaa gaccgtatt gtctcagtc gagcgaaca 
      atcggtgaa ttatgccac gttttcggg cagtattcc 
      cggaaagcg gcccgtctg agagacaaa ggggacgaa 
      attgccaaa gtcacgctc gaacacgct gaagcggaa 
      caactaaac cgatctcta agcctagct ttggagaat 
      tttgccgac tgtatctcg cttttgagt tcttacggc 
      gacctcaga gtccagaat ttggacagt aaagtgatt 
      aaagagttt gcacgctat gaggacatt tgtaaacat 
      gccaaagac gaagttaag caaatttac aacgctagg 
      gagaaagaa attgcgagg aaaagacag ctggatcgg 
      attaaagaa cgcaatccg aggaatagg caacaaatt 
      atccaagct gagaccgag ttagtaaag gcgagtgcc 
      gagttttcg aagactttg cgtaacttg gaggaaaaa 
      agcacggag tttgagaga caaaaactg cacgatgtt 
      aagtcgatt ttgctcgac tttgttgcg atagaaatg 
      gggtaccac gcccgggct ctggaggtc ttgaccaat 
      gcctacaaa gctgtgaat tcgatggac gaagaggcc 
      gacttacag gactttcaa aaaactagt ggaaaggtg 
      gaggcggag tttaaaaag tcgctgcgg cgcccggac 
      agttcacgc aacgtgggt tcgcgcagt ctttttcgc 
      tctagcaat tcgctgggt tccctcaag ggtcttttt 
      acccccaat aacaaaaaa ccagagggg attcccaga 
      aaagcgtcc aaaagtgag gaaacgctc gactccatg 
      aaacggagc atttccgac actgaggac agcctcagc 
      gacagtgcg aacgtctct gagaatgtt tccgacact 
      gaggagccc tcgtccccc atcagaccg cggaagccg 
      cgcaaatga aacattcgc atgcttagt tctaattta 
      ttaataaaa cggttcaat ggta
    • >TC013337 RB
      gcaaataaa aacaattca acaataatg ttgaacacg 
      ttccgggcg cggaataat tgcgaacag gaggctaaa 
      ttcgtccaa gaccgtatt gtctcagtc gagcgaaca 
      atcggtgaa ttatgccac gttttcggg cagtattcc 
      cggaaagcg gcccgtctg agagacaaa ggggacgaa 
      attgccaaa gtcacgctc gaacacgct gaagcggaa 
      caactaaac cgatctcta agcctagct ttggagaat 
      tttgccgac tgtatctcg cttttgagt tcttacggc 
      gacctcaga gtccagaat ttggacagt aaagtgatt 
      aaagagttt gcacgctat gaggacatt tgtaaacat 
      gccaaagac gaagttaag caaatttac aacgctagg 
      gagaaagaa attgcgagg aaaagacag ctggatcgg 
      attaaagaa cgcaatccg aggaatagg caacaaatt 
      atccaagct gagaccgag ttagtaaag gcgagtgcc 
      gagttttcg aagactttg cgtaacttg gaggaaaaa 
      agcacggag tttgagaga caaaaactg cacgatgtt 
      aagtcgatt ttgctcgac tttgttgcg atagaaatg 
      gggtaccac gcccgggct ctggaggtc ttgaccaat 
      gcctacaaa gctgtgaat tcgatggac gaagaggcc 
      gacttacag gagtttaaa aagtcgctg cggcgcccg 
      gacagttca cgcaacgtg ggttcgcgc agtcttttt 
      cgctctagc aattcgctg ggttccctc aagggtctt 
      tttaccccc aataacaaa aaaccagag gggattccc 
      agaaaagcg tccaaaagt gaggaaacg ctcgactcc 
      atgaaacgg agcatttcc gacactgag gacagcctc 
      agcgacagt gcgaacgtc tctgagaat gtttccgac 
      actgaggag ccctcgtcc cccatcaga ccgcggaag 
      ccgcgcaaa tgaaacatt cgcatgctt agttctaat 
      ttattaata aaacggttc aatggta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY