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Gene information
General information
TC032758
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007630
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2009253..2011297
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032758
      iBeetle screen iB_10722

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032758 RA
        MFSINRNSY LHTRSSSVN PRIINRCVD LARSQPTLH 
        DSSYFEPPF YRLTSEQPD IPCEFVDPT RRQVAFGKW 
        ALPKLRREL HDEDELVVI QALRSISDL VHDPEKAFE 
        AIRLRIPDR LCDKLLDPL PPVRENAAL ILAILAELA 
        DGKDAILRN DSILENLYL CLGDGQMSV RLKIASCFK 
        NIATLWTTA DVLVDYGLI QTLLEIIDK EEDLDIVLI 
        HLETLQYLM YECGKCVAL KNQGFRIFS KYLDHESPA 
        IVCKALDCL SILTSTKKG KKLALEKKL LKTLNRLLH 
        YGDVDICTS AASVVMFCT VKTRAKHAA AEIKPLPKR 
        LVKLATNPL NSTLQMFCL KALTNMCEH PDVRKEVRE 
        KYYDDLANL QVGSNEVYR KILLQIVGW VPHKWTM
      CDS sequence
      • >TC032758 RA
        atgttctca ataaaccgc aactcctac ctacataca 
        cgaagcagt tctgtaaat ccccgaatt ataaaccga 
        tgcgttgac ttggcgaga agtcaaccc acccttcat 
        gactcctct tacttcgaa ccccccttt taccgcctg 
        acttccgaa cagcccgat attccctgc gaatttgtt 
        gacccgacc agaagacaa gtcgcattc ggaaaatgg 
        gctttaccc aaacttaga cgcgaattg cacgacgaa 
        gacgaatta gtcgtaatt caggcactt cgcagcatt 
        agcgacttg gtacacgac ccggaaaag gccttcgaa 
        gctattagg ctccgcatt ccggataga ttgtgtgac 
        aaactcctt gatcctctg cctcccgtg cgtgaaaac 
        gcagctttg attctcgcc atcttggcc gaattggcc 
        gatggaaaa gacgcgatt ttacgaaat gattcaatt 
        ttggaaaat ttatactta tgtctaggt gatggacaa 
        atgtcggtg cggcttaaa atcgcttcg tgtttcaaa 
        aatattgcc acgctttgg accaccgcc gatgttttg 
        gttgattac ggactcata caaactctt ctggaaata 
        atcgataaa gaagaagac ctcgatatt gtcctaatc 
        cacttagaa acactccaa tatttaatg tacgaatgc 
        ggcaaatgt gtggctttg aaaaatcaa ggtttccga 
        attttcagc aaatatctg gatcatgaa tcaccggcg 
        atcgtttgc aaagcgctt gactgcctt tcgatttta 
        acctcgaca aagaaaggc aaaaagctc gctcttgag 
        aagaaacta ttgaaaaca ttaaaccga ctattacat 
        tacggggac gttgatata tgcacaagt gcggcttct 
        gtcgtcatg ttctgcact gttaagaca cgagcaaaa 
        catgcagcg gccgaaatt aagcctctc cccaaacgt 
        ttggtcaaa ctcgccaca aatccgcta aatagcact 
        ttgcaaatg ttttgcttg aaggccttg acaaatatg 
        tgtgaacat cccgacgtc aggaaagaa gtgcgtgag 
        aaatattac gacgatctg gcgaattta caagtcggg 
        agtaacgaa gtctacaga aagattctt ctgcaaata 
        gtcggttgg gtgccacac aaatggacc atgtaa
      mRNA sequence
      • >TC032758 RA
        aataacatt gttccattt ttcttaaaa aaatggaaa 
        aaatcgtga caataggat ttttttttg actgttttt 
        gacgttgac atttttgtc atattattt tttcaactt 
        ttgaaatgt tctcaataa accgcaact cctacctac 
        atacacgaa gcagttctg taaatcccc gaattataa 
        accgatgcg ttgacttgg cgagaagtc aacccaccc 
        ttcatgact cctcttact tcgaacccc ccttttacc 
        gcctgactt ccgaacagc ccgatattc cctgcgaat 
        ttgttgacc cgaccagaa gacaagtcg cattcggaa 
        aatgggctt tacccaaac ttagacgcg aattgcacg 
        acgaagacg aattagtcg taattcagg cacttcgca 
        gcattagcg acttggtac acgacccgg aaaaggcct 
        tcgaagcta ttaggctcc gcattccgg atagattgt 
        gtgacaaac tccttgatc ctctgcctc ccgtgcgtg 
        aaaacgcag ctttgattc tcgccatct tggccgaat 
        tggccgatg gaaaagacg cgattttac gaaatgatt 
        caattttgg aaaatttat acttatgtc taggtgatg 
        gacaaatgt cggtgcggc ttaaaatcg cttcgtgtt 
        tcaaaaata ttgccacgc tttggacca ccgccgatg 
        ttttggttg attacggac tcatacaaa ctcttctgg 
        aaataatcg ataaagaag aagacctcg atattgtcc 
        taatccact tagaaacac tccaatatt taatgtacg 
        aatgcggca aatgtgtgg ctttgaaaa atcaaggtt 
        tccgaattt tcagcaaat atctggatc atgaatcac 
        cggcgatcg tttgcaaag cgcttgact gcctttcga 
        ttttaacct cgacaaaga aaggcaaaa agctcgctc 
        ttgagaaga aactattga aaacattaa accgactat 
        tacattacg gggacgttg atatatgca caagtgcgg 
        cttctgtcg tcatgttct gcactgtta agacacgag 
        caaaacatg cagcggccg aaattaagc ctctcccca 
        aacgtttgg tcaaactcg ccacaaatc cgctaaata 
        gcactttgc aaatgtttt gcttgaagg ccttgacaa 
        atatgtgtg aacatcccg acgtcagga aagaagtgc 
        gtgagaaat attacgacg atctggcga atttacaag 
        tcgggagta acgaagtct acagaaaga ttcttctgc 
        aaatagtcg gttgggtgc cacacaaat ggaccatgt 
        aatgaataa aaaaataaa taaattctt gtgtgatac 
        agatatctg tttactcga aatactggg gtccaatta 
        tcgtgttta caagtgcat caggtgata agttttccg 
        ataagctcg tgtttgcat atccacagc aaacaaaac 
        attttttcc acaaaaatc aatattaca ataagatgt 
        tatcaattg tagtagcta tttttggat aaagtaagc 
        agagcgttt gtaaaaaaa taaaaagga gttttttga 
        acgatttac tcaacgaaa tcgacgtgg ggccatcgg 
        ggtgtctat ttatagtat atttacaag cacgtgtct 
        tgttaaaaa tcgcaaata atcttctcg gtctgcaca 
        gcaggaagt gataacgtg cagctttaa tttttcgat 
        tcatttaag caataaagt gtttaatga taa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY