Status
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Gene information
General information
TC005008 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 642978..656748
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC005008
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10718

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Adult morphology
      abdominal odoriferous glands content not present - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection


      abdominal odoriferous glands content darker - Number of animals showing the phenotype: 2 (13 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva complete potentiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: Constriction often occours between head and thorax segment.
      legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothoracic leg potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothorax segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothoracic leg not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
      Comment: Constriction often occours between head and thorax segment.
      thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005008 RA
        MDICTTPPP TEPTIHMSP PLPPVIASP 
SPPPPPSIS DCKTSKKKK CDIPLPPEP 
KKDVSSERY ASNGEPFPP GVDASDVTT 
KTKDIKGND MTDVLNSFY SDIAAIETS 
NSPPVSNVN EDTKLESTV DNVQEPMKK 
KKKTKVKLA QGIAMKKKG VSKLVEKWK 
NVQLSYND
      • >TC005008 RB
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
VLISSYSGD SESEEEKEE ANTKKLVSI SLKQTIYLK 
INGSKPLLT SEDNDEVTK QKTSTEPTS KASIVPKKP 
ENKEISGFS LVAGYSDSD EEQEESNHK PNFLIGPPP 
QENKVSHST LFPATKPID VKDFAESPK IIGEGDEGF 
DSKAFQRKR RIGVALINT GKKTVIDDD NERKGLGFS 
NDSNSGENS SSPYPGFKS GGVMFVKAD VLNPTSTEE 
KAEDCSLIE ETYTTLKEK LGFLSEGRE EVSPVQVML 
IQVETLYIA LKSGNLKHT YLKQWLDEK CSELIKLEK 
EATPEGWLL QWDRSHKRY FYQNASTGE SQWEYPQAD 
VTVCDEAMD ICTTPPPTE PTIHMSPPL PPVIASPSP 
PPPPSISDC KTSKKKKCD IPLPPEPKK DVSSERYAS 
NGEPFPPGV DASDVTTKT KDIKGNDMT DVLNSFYSD 
IAAIETSNS PPVSNVNED TKLESTVDN VQEPMKKKK 
KTKVKLAQG IAMKKKGVS KLVEKWKNV QLSYND
      • >TC005008 RC
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
VLISSYSGD SESEEEKEE ANTKKLVSI SLKQTIYLK 
INGSKPLLT SEDNDEVTK QKTSTEPTS KASIVPKKP 
ENKEISGFS LVAGYSDSD EEQEESNHK PNFLIGPPP 
QENKVSHST LFPATKPID VKDFAESPK IIGEGDEGF 
DSKAFQRKR RIGVALINT GKKTVIDDD NERKGLGFS 
NDSNSGENS SSPYPGFKS GGVMFVKAD VLNPTSTEE 
KAEDCSLIE ETYTTLKEK LGFLSEGRE EVSPVQVML 
IQVETLYIA LKSGNLKHT YLKQWLDEK CSELIKLEK 
EATPEGWLL QWDRYETCN FLFIVSVFF THFFFILWP 
SF
      • >TC005008 RD
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
VLISSYSGD SESEEEKEE ANTKKLVSH TFII
      CDS sequence
      • >TC005008 RA
        atggatatt tgtacaaca cctcctcca acagaacca 
acaattcat atgtcgcct cctttgcct ccagtcatc 
gctagtccg agtccgccg ccaccacct agcattagt 
gactgcaag accagtaaa aaaaagaaa tgtgatatt 
cctctccct cctgagcca aaaaaagat gtatctagt 
gaaaggtat gccagcaat ggggaacca tttcctcca 
ggtgtggat gcgtctgat gtgacaaca aaaacaaag 
gatattaaa ggaaatgat atgaccgat gttctaaac 
tcgttttat tcagatatt gctgcaatt gaaactagt 
aattcacca cctgtttct aatgttaac gaagataca 
aaattagag tcgactgtg gacaatgtt caagaaccg 
atgaagaaa aagaagaaa acgaaagtg aaattggca 
caaggaata gctatgaag aagaaaggg gtgtcgaaa 
ttggtggaa aaatggaag aatgtacaa ttgtcatat 
aatgattga
      • >TC005008 RB
        atggatgca agagtgaac gactttctt aacgaaatt 
gacgaaata gccccactt agcgaaaac aaggaggat 
tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
tattattat tactggaac actaaaaca aacgaagta 
acgtgggat atgcccgcc gaatacaaa tcattgaac 
aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
cctccacga acagccccc ttgtttcca agcacacgt 
aacttgtta ccgcctgta tcagatgcc ataaaaatt 
tataaaatt ggtgaatgt gctgggagt agtacaaag 
agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
aaagaagag aaaaagact gtggtgaaa actaaaccg 
gttaggaaa cacagtgat tctgatgat gaaaaaatt 
gttttgatc tcatcttat agtggcgac agtgaatca 
gaggaggaa aaggaggaa gcaaataca aaaaaattg 
gtaagcata tctttaaaa caaacaata tatttgaag 
ataaatgga tcaaagccg ctccttact tcagaagat 
aatgacgaa gttacgaaa cagaaaaca tcaactgaa 
cctacgagt aaagcttca attgtaccg aaaaaacct 
gaaaacaag gaaatttcc ggtttttca ctcgttgcc 
ggttatagt gactccgat gaagagcag gaagaatca 
aaccacaag cctaatttt ttaatagga ccacctcct 
caagaaaat aaagtttct catagcacc ctttttccc 
gccactaaa ccaatagac gttaaagat tttgctgaa 
tcgccaaaa ataatcgga gaaggtgat gaaggtttt 
gattcaaag gcgtttcaa cgtaaacgc cgaattgga 
gttgcgtta ataaatacc ggtaaaaaa acagtaatt 
gatgatgat aacgaacga aaaggactg ggattttct 
aatgatagc aatagtggt gagaattca agcagtccg 
tatcccgga tttaaaagt ggaggtgtt atgtttgtc 
aaagctgat gttttaaat ccaacgagt acagaagag 
aaagctgaa gattgtagt ttgattgaa gaaacgtac 
acgacattg aaagaaaaa ttaggattt ttaagtgaa 
ggtcgagaa gaggtttca cctgttcaa gtgatgctt 
attcaagtt gagactctc tacatcgca ctcaagtcg 
ggaaattta aaacacacc tatttaaag caatggctt 
gatgagaaa tgctccgaa ctcattaaa cttgaaaaa 
gaagcgact ccagaaggt tggcttctt caatgggat 
aggtcacat aagcgctat ttttatcaa aatgcgtct 
acgggggag agtcaatgg gaatatccc caagccgat 
gttacagtg tgtgatgaa gctatggat atttgtaca 
acacctcct ccaacagaa ccaacaatt catatgtcg 
cctcctttg cctccagtc atcgctagt ccgagtccg 
ccgccacca cctagcatt agtgactgc aagaccagt 
aaaaaaaag aaatgtgat attcctctc cctcctgag 
ccaaaaaaa gatgtatct agtgaaagg tatgccagc 
aatggggaa ccatttcct ccaggtgtg gatgcgtct 
gatgtgaca acaaaaaca aaggatatt aaaggaaat 
gatatgacc gatgttcta aactcgttt tattcagat 
attgctgca attgaaact agtaattca ccacctgtt 
tctaatgtt aacgaagat acaaaatta gagtcgact 
gtggacaat gttcaagaa ccgatgaag aaaaagaag 
aaaacgaaa gtgaaattg gcacaagga atagctatg 
aagaagaaa ggggtgtcg aaattggtg gaaaaatgg 
aagaatgta caattgtca tataatgat tga
      • >TC005008 RC
        atggatgca agagtgaac gactttctt aacgaaatt 
gacgaaata gccccactt agcgaaaac aaggaggat 
tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
tattattat tactggaac actaaaaca aacgaagta 
acgtgggat atgcccgcc gaatacaaa tcattgaac 
aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
cctccacga acagccccc ttgtttcca agcacacgt 
aacttgtta ccgcctgta tcagatgcc ataaaaatt 
tataaaatt ggtgaatgt gctgggagt agtacaaag 
agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
aaagaagag aaaaagact gtggtgaaa actaaaccg 
gttaggaaa cacagtgat tctgatgat gaaaaaatt 
gttttgatc tcatcttat agtggcgac agtgaatca 
gaggaggaa aaggaggaa gcaaataca aaaaaattg 
gtaagcata tctttaaaa caaacaata tatttgaag 
ataaatgga tcaaagccg ctccttact tcagaagat 
aatgacgaa gttacgaaa cagaaaaca tcaactgaa 
cctacgagt aaagcttca attgtaccg aaaaaacct 
gaaaacaag gaaatttcc ggtttttca ctcgttgcc 
ggttatagt gactccgat gaagagcag gaagaatca 
aaccacaag cctaatttt ttaatagga ccacctcct 
caagaaaat aaagtttct catagcacc ctttttccc 
gccactaaa ccaatagac gttaaagat tttgctgaa 
tcgccaaaa ataatcgga gaaggtgat gaaggtttt 
gattcaaag gcgtttcaa cgtaaacgc cgaattgga 
gttgcgtta ataaatacc ggtaaaaaa acagtaatt 
gatgatgat aacgaacga aaaggactg ggattttct 
aatgatagc aatagtggt gagaattca agcagtccg 
tatcccgga tttaaaagt ggaggtgtt atgtttgtc 
aaagctgat gttttaaat ccaacgagt acagaagag 
aaagctgaa gattgtagt ttgattgaa gaaacgtac 
acgacattg aaagaaaaa ttaggattt ttaagtgaa 
ggtcgagaa gaggtttca cctgttcaa gtgatgctt 
attcaagtt gagactctc tacatcgca ctcaagtcg 
ggaaattta aaacacacc tatttaaag caatggctt 
gatgagaaa tgctccgaa ctcattaaa cttgaaaaa 
gaagcgact ccagaaggt tggcttctt caatgggat 
aggtacgaa acgtgcaat tttttattt atagtttca 
gtttttttc acccacttt ttctttatc ctgtggcca 
tccttttaa
      • >TC005008 RD
        atggatgca agagtgaac gactttctt aacgaaatt 
gacgaaata gccccactt agcgaaaac aaggaggat 
tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
tattattat tactggaac actaaaaca aacgaagta 
acgtgggat atgcccgcc gaatacaaa tcattgaac 
aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
cctccacga acagccccc ttgtttcca agcacacgt 
aacttgtta ccgcctgta tcagatgcc ataaaaatt 
tataaaatt ggtgaatgt gctgggagt agtacaaag 
agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
aaagaagag aaaaagact gtggtgaaa actaaaccg 
gttaggaaa cacagtgat tctgatgat gaaaaaatt 
gttttgatc tcatcttat agtggcgac agtgaatca 
gaggaggaa aaggaggaa gcaaataca aaaaaattg 
gtaagtcat acttttatt atctaa
      mRNA sequence
      • >TC005008 RA
        ttaaaacaa acaatatat ttgaagata aatggatca 
aagccgctc cttacttca gaagataat gacgaagtt 
acgaaacag aaaacatca actgaacct acgagtaaa 
gcttcaatt gtaccgaaa aaacctgaa aacaaggaa 
atttccggt ttttcactc gttgccggt tatagtgac 
tccgatgaa gagcaggaa gaatcaaac cacaagcct 
aatttttta ataggacca cctcctcaa gaaaataaa 
gtttctcat agcaccctt tttcccgcc actaaacca 
atagacgtt aaagatttt gctgaatcg ccaaaaata 
atcggagaa ggtgatgaa ggttttgat tcaaaggcg 
tttcaacgt aaacgccga attggagtt gcgttaata 
aataccggt aaaaaaaca gtaattgat gatgataac 
gaacgaaaa ggactggga ttttctaat gatagcaat 
agtggtgag aattcaagc agtccgtat cccggattt 
aaaagtgga ggtgttatg tttgtcaaa gctgatgtt 
ttaaatcca acgagtaca gaagagaaa gctgaagat 
tgtagtttg attgaagaa acgtacacg acattgaaa 
gaaaaatta ggattttta agtgaaggt cgagaagag 
gtttcacct gttcaagtg atgcttatt caagttgag 
actctctac atcgcactc aagtcggga aatttaaaa 
cacacctat ttaaagcaa tggcttgat gagaaatgc 
tccgaactc attaaactt gaaaaagaa gcgactcca 
gaaggttgg cttcttcaa tgggatagg tacgaaacg 
tgcaatttt ttatttata gtttcagtt tttttcacc 
cactttttc tttatcctg tggccatcc ttttaacct 
tattattag ctccatttt ttctgctgt ttcccctcg 
cagccaaca tcgagatat gcagtcaat tacttcagg 
taagaatta ttttaattt tcatttaat tgatttgac 
taaaaacga gctagaacg tatagtttt gacattgtt 
gaaataaaa tcgaagatg cagttattc caattgtat 
gaggcattt tttctgtag ttttaaatc aattcaggt 
tcgtaaatt tattttcac ttgagttca ttacgtttt 
gttcacttt tattaaagt ttatttatc acgtttaaa 
tgagtggat ttaatgaaa tgaactcgt ttatccatt 
taatagttt gattaattg caattgtat taatctttt 
tttgtgtgt gtaggacat gaagattgg tggtattcc 
tgtggtccg aacgcgaag ctggattta tttatctta 
aatttctaa acttaaatt agtgtgtaa taataactg 
ttttgcggt gtcgcaggt atagtatat gcagaaaag 
tgaaaaaag taaaattgg aagagaaaa cttaaaaaa 
agattcatc aatttcctt gtgtgttaa ttaacttga 
tgaatctta tttatcatt tttccgcgc ctaaaaaat 
cacttgacg acatcaagt caaaactgt gttctgtga 
ggtggtttt ggtgatgtt ttaggtcac ataagcgct 
atttttatc aaaatgcgt ctacggggg agagtcaat 
gggaatatc cccaagccg atgttacag tgtgtgatg 
aagctatgg atatttgta caacacctc ctccaacag 
aaccaacaa ttcatatgt cgcctcctt tgcctccag 
tcatcgcta gtccgagtc cgccgccac cacctagca 
ttagtgact gcaagacca gtaaaaaaa agaaatgtg 
atattcctc tccctcctg agccaaaaa aagatgtat 
ctagtgaaa ggtatgcca gcaatgggg aaccatttc 
ctccaggtg tggatgcgt ctgatgtga caacaaaaa 
caaaggata ttaaaggaa atgatatga ccgatgttc 
taaactcgt tttattcag atattgctg caattgaaa 
ctagtaatt caccacctg tttctaatg ttaacgaag 
atacaaaat tagagtcga ctgtggaca atgttcaag 
aaccgatga agaaaaaga agaaaacga aagtgaaat 
tggcacaag gaatagcta tgaagaaga aaggggtgt 
cgaaattgg tggaaaaat ggaagaatg tacaattgt 
catataatg attgaatgc aaaagtttt tttacctaa 
ataataaag tagagatca aact
      • >TC005008 RB
        tgtcagatg gccgaacat aacctataa aaagttgat 
tattttatt atttttgtg cataattta acaatgtgt 
taaatgaaa atggatgca agagtgaac gactttctt 
aacgaaatt gacgaaata gccccactt agcgaaaac 
aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
gagagcggt tattattat tactggaac actaaaaca 
aacgaagta acgtgggat atgcccgcc gaatacaaa 
tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
ttgtatatc cctccacga acagccccc ttgtttcca 
agcacacgt aacttgtta ccgcctgta tcagatgcc 
ataaaaatt tataaaatt ggtgaatgt gctgggagt 
agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
cataaagtt aaagaagag aaaaagact gtggtgaaa 
actaaaccg gttaggaaa cacagtgat tctgatgat 
gaaaaaatt gttttgatc tcatcttat agtggcgac 
agtgaatca gaggaggaa aaggaggaa gcaaataca 
aaaaaattg gtaagcata tctttaaaa caaacaata 
tatttgaag ataaatgga tcaaagccg ctccttact 
tcagaagat aatgacgaa gttacgaaa cagaaaaca 
tcaactgaa cctacgagt aaagcttca attgtaccg 
aaaaaacct gaaaacaag gaaatttcc ggtttttca 
ctcgttgcc ggttatagt gactccgat gaagagcag 
gaagaatca aaccacaag cctaatttt ttaatagga 
ccacctcct caagaaaat aaagtttct catagcacc 
ctttttccc gccactaaa ccaatagac gttaaagat 
tttgctgaa tcgccaaaa ataatcgga gaaggtgat 
gaaggtttt gattcaaag gcgtttcaa cgtaaacgc 
cgaattgga gttgcgtta ataaatacc ggtaaaaaa 
acagtaatt gatgatgat aacgaacga aaaggactg 
ggattttct aatgatagc aatagtggt gagaattca 
agcagtccg tatcccgga tttaaaagt ggaggtgtt 
atgtttgtc aaagctgat gttttaaat ccaacgagt 
acagaagag aaagctgaa gattgtagt ttgattgaa 
gaaacgtac acgacattg aaagaaaaa ttaggattt 
ttaagtgaa ggtcgagaa gaggtttca cctgttcaa 
gtgatgctt attcaagtt gagactctc tacatcgca 
ctcaagtcg ggaaattta aaacacacc tatttaaag 
caatggctt gatgagaaa tgctccgaa ctcattaaa 
cttgaaaaa gaagcgact ccagaaggt tggcttctt 
caatgggat aggtcacat aagcgctat ttttatcaa 
aatgcgtct acgggggag agtcaatgg gaatatccc 
caagccgat gttacagtg tgtgatgaa gctatggat 
atttgtaca acacctcct ccaacagaa ccaacaatt 
catatgtcg cctcctttg cctccagtc atcgctagt 
ccgagtccg ccgccacca cctagcatt agtgactgc 
aagaccagt aaaaaaaag aaatgtgat attcctctc 
cctcctgag ccaaaaaaa gatgtatct agtgaaagg 
tatgccagc aatggggaa ccatttcct ccaggtgtg 
gatgcgtct gatgtgaca acaaaaaca aaggatatt 
aaaggaaat gatatgacc gatgttcta aactcgttt 
tattcagat attgctgca attgaaact agtaattca 
ccacctgtt tctaatgtt aacgaagat acaaaatta 
gagtcgact gtggacaat gttcaagaa ccgatgaag 
aaaaagaag aaaacgaaa gtgaaattg gcacaagga 
atagctatg aagaagaaa ggggtgtcg aaattggtg 
gaaaaatgg aagaatgta caattgtca tataatgat 
tgaatgcaa aagtttttt tacctaaat aataaagta 
gagatcaaa ct
      • >TC005008 RC
        tgtcagatg gccgaacat aacctataa aaagttgat 
tattttatt atttttgtg cataattta acaatgtgt 
taaatgaaa atggatgca agagtgaac gactttctt 
aacgaaatt gacgaaata gccccactt agcgaaaac 
aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
gagagcggt tattattat tactggaac actaaaaca 
aacgaagta acgtgggat atgcccgcc gaatacaaa 
tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
ttgtatatc cctccacga acagccccc ttgtttcca 
agcacacgt aacttgtta ccgcctgta tcagatgcc 
ataaaaatt tataaaatt ggtgaatgt gctgggagt 
agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
cataaagtt aaagaagag aaaaagact gtggtgaaa 
actaaaccg gttaggaaa cacagtgat tctgatgat 
gaaaaaatt gttttgatc tcatcttat agtggcgac 
agtgaatca gaggaggaa aaggaggaa gcaaataca 
aaaaaattg gtaagcata tctttaaaa caaacaata 
tatttgaag ataaatgga tcaaagccg ctccttact 
tcagaagat aatgacgaa gttacgaaa cagaaaaca 
tcaactgaa cctacgagt aaagcttca attgtaccg 
aaaaaacct gaaaacaag gaaatttcc ggtttttca 
ctcgttgcc ggttatagt gactccgat gaagagcag 
gaagaatca aaccacaag cctaatttt ttaatagga 
ccacctcct caagaaaat aaagtttct catagcacc 
ctttttccc gccactaaa ccaatagac gttaaagat 
tttgctgaa tcgccaaaa ataatcgga gaaggtgat 
gaaggtttt gattcaaag gcgtttcaa cgtaaacgc 
cgaattgga gttgcgtta ataaatacc ggtaaaaaa 
acagtaatt gatgatgat aacgaacga aaaggactg 
ggattttct aatgatagc aatagtggt gagaattca 
agcagtccg tatcccgga tttaaaagt ggaggtgtt 
atgtttgtc aaagctgat gttttaaat ccaacgagt 
acagaagag aaagctgaa gattgtagt ttgattgaa 
gaaacgtac acgacattg aaagaaaaa ttaggattt 
ttaagtgaa ggtcgagaa gaggtttca cctgttcaa 
gtgatgctt attcaagtt gagactctc tacatcgca 
ctcaagtcg ggaaattta aaacacacc tatttaaag 
caatggctt gatgagaaa tgctccgaa ctcattaaa 
cttgaaaaa gaagcgact ccagaaggt tggcttctt 
caatgggat aggtacgaa acgtgcaat tttttattt 
atagtttca gtttttttc acccacttt ttctttatc 
ctgtggcca tccttttaa ccttattat tagctccat 
tttttctgc tgtttcccc tcgcagcca acatcgaga 
tatgcagtc aattacttc aggtaagaa ttattttaa 
ttttcattt aattgattt gactaaaaa cgagctaga 
acgtatagt tttgacatt gttgaaata aaatcgaag 
atgcagt
      • >TC005008 RD
        tgtcagatg gccgaacat aacctataa aaagttgat 
tattttatt atttttgtg cataattta acaatgtgt 
taaatgaaa atggatgca agagtgaac gactttctt 
aacgaaatt gacgaaata gccccactt agcgaaaac 
aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
gagagcggt tattattat tactggaac actaaaaca 
aacgaagta acgtgggat atgcccgcc gaatacaaa 
tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
ttgtatatc cctccacga acagccccc ttgtttcca 
agcacacgt aacttgtta ccgcctgta tcagatgcc 
ataaaaatt tataaaatt ggtgaatgt gctgggagt 
agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
cataaagtt aaagaagag aaaaagact gtggtgaaa 
actaaaccg gttaggaaa cacagtgat tctgatgat 
gaaaaaatt gttttgatc tcatcttat agtggcgac 
agtgaatca gaggaggaa aaggaggaa gcaaataca 
aaaaaattg gtaagtcat acttttatt atctaaaaa 
ttagttttt gaaaaacaa attgaataa aataagcca 
aaataa
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