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Gene information
General information
TC005008 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 642978..656748
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005008
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10718

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Adult morphology
      abdominal odoriferous glands content not present - Number of animals showing the phenotype: 4 (13 dpi)
      Days post injection



      abdominal odoriferous glands content darker - Number of animals showing the phenotype: 2 (13 dpi)
      Days post injection



      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva complete potentiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      Comment: Constriction often occours between head and thorax segment.
      legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothoracic leg potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothorax segment not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      prothoracic leg not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
      Comment: Constriction often occours between head and thorax segment.
      thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005008 RA
        MDICTTPPP TEPTIHMSP PLPPVIASP 
        SPPPPPSIS DCKTSKKKK CDIPLPPEP 
        KKDVSSERY ASNGEPFPP GVDASDVTT 
        KTKDIKGND MTDVLNSFY SDIAAIETS 
        NSPPVSNVN EDTKLESTV DNVQEPMKK 
        KKKTKVKLA QGIAMKKKG VSKLVEKWK 
        NVQLSYND
      • >TC005008 RB
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
        YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
        PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
        SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
        VLISSYSGD SESEEEKEE ANTKKLVSI SLKQTIYLK 
        INGSKPLLT SEDNDEVTK QKTSTEPTS KASIVPKKP 
        ENKEISGFS LVAGYSDSD EEQEESNHK PNFLIGPPP 
        QENKVSHST LFPATKPID VKDFAESPK IIGEGDEGF 
        DSKAFQRKR RIGVALINT GKKTVIDDD NERKGLGFS 
        NDSNSGENS SSPYPGFKS GGVMFVKAD VLNPTSTEE 
        KAEDCSLIE ETYTTLKEK LGFLSEGRE EVSPVQVML 
        IQVETLYIA LKSGNLKHT YLKQWLDEK CSELIKLEK 
        EATPEGWLL QWDRSHKRY FYQNASTGE SQWEYPQAD 
        VTVCDEAMD ICTTPPPTE PTIHMSPPL PPVIASPSP 
        PPPPSISDC KTSKKKKCD IPLPPEPKK DVSSERYAS 
        NGEPFPPGV DASDVTTKT KDIKGNDMT DVLNSFYSD 
        IAAIETSNS PPVSNVNED TKLESTVDN VQEPMKKKK 
        KTKVKLAQG IAMKKKGVS KLVEKWKNV QLSYND
      • >TC005008 RC
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
        YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
        PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
        SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
        VLISSYSGD SESEEEKEE ANTKKLVSI SLKQTIYLK 
        INGSKPLLT SEDNDEVTK QKTSTEPTS KASIVPKKP 
        ENKEISGFS LVAGYSDSD EEQEESNHK PNFLIGPPP 
        QENKVSHST LFPATKPID VKDFAESPK IIGEGDEGF 
        DSKAFQRKR RIGVALINT GKKTVIDDD NERKGLGFS 
        NDSNSGENS SSPYPGFKS GGVMFVKAD VLNPTSTEE 
        KAEDCSLIE ETYTTLKEK LGFLSEGRE EVSPVQVML 
        IQVETLYIA LKSGNLKHT YLKQWLDEK CSELIKLEK 
        EATPEGWLL QWDRYETCN FLFIVSVFF THFFFILWP 
        SF
      • >TC005008 RD
        MDARVNDFL NEIDEIAPL SENKEDCAW EKCYDEESG 
        YYYYWNTKT NEVTWDMPA EYKSLNKTK SKKTCNLYI 
        PPRTAPLFP STRNLLPPV SDAIKIYKI GECAGSSTK 
        SEKVVKGEE HKVKEEKKT VVKTKPVRK HSDSDDEKI 
        VLISSYSGD SESEEEKEE ANTKKLVSH TFII
      CDS sequence
      • >TC005008 RA
        atggatatt tgtacaaca cctcctcca acagaacca 
        acaattcat atgtcgcct cctttgcct ccagtcatc 
        gctagtccg agtccgccg ccaccacct agcattagt 
        gactgcaag accagtaaa aaaaagaaa tgtgatatt 
        cctctccct cctgagcca aaaaaagat gtatctagt 
        gaaaggtat gccagcaat ggggaacca tttcctcca 
        ggtgtggat gcgtctgat gtgacaaca aaaacaaag 
        gatattaaa ggaaatgat atgaccgat gttctaaac 
        tcgttttat tcagatatt gctgcaatt gaaactagt 
        aattcacca cctgtttct aatgttaac gaagataca 
        aaattagag tcgactgtg gacaatgtt caagaaccg 
        atgaagaaa aagaagaaa acgaaagtg aaattggca 
        caaggaata gctatgaag aagaaaggg gtgtcgaaa 
        ttggtggaa aaatggaag aatgtacaa ttgtcatat 
        aatgattga
      • >TC005008 RB
        atggatgca agagtgaac gactttctt aacgaaatt 
        gacgaaata gccccactt agcgaaaac aaggaggat 
        tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
        tattattat tactggaac actaaaaca aacgaagta 
        acgtgggat atgcccgcc gaatacaaa tcattgaac 
        aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
        cctccacga acagccccc ttgtttcca agcacacgt 
        aacttgtta ccgcctgta tcagatgcc ataaaaatt 
        tataaaatt ggtgaatgt gctgggagt agtacaaag 
        agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
        aaagaagag aaaaagact gtggtgaaa actaaaccg 
        gttaggaaa cacagtgat tctgatgat gaaaaaatt 
        gttttgatc tcatcttat agtggcgac agtgaatca 
        gaggaggaa aaggaggaa gcaaataca aaaaaattg 
        gtaagcata tctttaaaa caaacaata tatttgaag 
        ataaatgga tcaaagccg ctccttact tcagaagat 
        aatgacgaa gttacgaaa cagaaaaca tcaactgaa 
        cctacgagt aaagcttca attgtaccg aaaaaacct 
        gaaaacaag gaaatttcc ggtttttca ctcgttgcc 
        ggttatagt gactccgat gaagagcag gaagaatca 
        aaccacaag cctaatttt ttaatagga ccacctcct 
        caagaaaat aaagtttct catagcacc ctttttccc 
        gccactaaa ccaatagac gttaaagat tttgctgaa 
        tcgccaaaa ataatcgga gaaggtgat gaaggtttt 
        gattcaaag gcgtttcaa cgtaaacgc cgaattgga 
        gttgcgtta ataaatacc ggtaaaaaa acagtaatt 
        gatgatgat aacgaacga aaaggactg ggattttct 
        aatgatagc aatagtggt gagaattca agcagtccg 
        tatcccgga tttaaaagt ggaggtgtt atgtttgtc 
        aaagctgat gttttaaat ccaacgagt acagaagag 
        aaagctgaa gattgtagt ttgattgaa gaaacgtac 
        acgacattg aaagaaaaa ttaggattt ttaagtgaa 
        ggtcgagaa gaggtttca cctgttcaa gtgatgctt 
        attcaagtt gagactctc tacatcgca ctcaagtcg 
        ggaaattta aaacacacc tatttaaag caatggctt 
        gatgagaaa tgctccgaa ctcattaaa cttgaaaaa 
        gaagcgact ccagaaggt tggcttctt caatgggat 
        aggtcacat aagcgctat ttttatcaa aatgcgtct 
        acgggggag agtcaatgg gaatatccc caagccgat 
        gttacagtg tgtgatgaa gctatggat atttgtaca 
        acacctcct ccaacagaa ccaacaatt catatgtcg 
        cctcctttg cctccagtc atcgctagt ccgagtccg 
        ccgccacca cctagcatt agtgactgc aagaccagt 
        aaaaaaaag aaatgtgat attcctctc cctcctgag 
        ccaaaaaaa gatgtatct agtgaaagg tatgccagc 
        aatggggaa ccatttcct ccaggtgtg gatgcgtct 
        gatgtgaca acaaaaaca aaggatatt aaaggaaat 
        gatatgacc gatgttcta aactcgttt tattcagat 
        attgctgca attgaaact agtaattca ccacctgtt 
        tctaatgtt aacgaagat acaaaatta gagtcgact 
        gtggacaat gttcaagaa ccgatgaag aaaaagaag 
        aaaacgaaa gtgaaattg gcacaagga atagctatg 
        aagaagaaa ggggtgtcg aaattggtg gaaaaatgg 
        aagaatgta caattgtca tataatgat tga
      • >TC005008 RC
        atggatgca agagtgaac gactttctt aacgaaatt 
        gacgaaata gccccactt agcgaaaac aaggaggat 
        tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
        tattattat tactggaac actaaaaca aacgaagta 
        acgtgggat atgcccgcc gaatacaaa tcattgaac 
        aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
        cctccacga acagccccc ttgtttcca agcacacgt 
        aacttgtta ccgcctgta tcagatgcc ataaaaatt 
        tataaaatt ggtgaatgt gctgggagt agtacaaag 
        agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
        aaagaagag aaaaagact gtggtgaaa actaaaccg 
        gttaggaaa cacagtgat tctgatgat gaaaaaatt 
        gttttgatc tcatcttat agtggcgac agtgaatca 
        gaggaggaa aaggaggaa gcaaataca aaaaaattg 
        gtaagcata tctttaaaa caaacaata tatttgaag 
        ataaatgga tcaaagccg ctccttact tcagaagat 
        aatgacgaa gttacgaaa cagaaaaca tcaactgaa 
        cctacgagt aaagcttca attgtaccg aaaaaacct 
        gaaaacaag gaaatttcc ggtttttca ctcgttgcc 
        ggttatagt gactccgat gaagagcag gaagaatca 
        aaccacaag cctaatttt ttaatagga ccacctcct 
        caagaaaat aaagtttct catagcacc ctttttccc 
        gccactaaa ccaatagac gttaaagat tttgctgaa 
        tcgccaaaa ataatcgga gaaggtgat gaaggtttt 
        gattcaaag gcgtttcaa cgtaaacgc cgaattgga 
        gttgcgtta ataaatacc ggtaaaaaa acagtaatt 
        gatgatgat aacgaacga aaaggactg ggattttct 
        aatgatagc aatagtggt gagaattca agcagtccg 
        tatcccgga tttaaaagt ggaggtgtt atgtttgtc 
        aaagctgat gttttaaat ccaacgagt acagaagag 
        aaagctgaa gattgtagt ttgattgaa gaaacgtac 
        acgacattg aaagaaaaa ttaggattt ttaagtgaa 
        ggtcgagaa gaggtttca cctgttcaa gtgatgctt 
        attcaagtt gagactctc tacatcgca ctcaagtcg 
        ggaaattta aaacacacc tatttaaag caatggctt 
        gatgagaaa tgctccgaa ctcattaaa cttgaaaaa 
        gaagcgact ccagaaggt tggcttctt caatgggat 
        aggtacgaa acgtgcaat tttttattt atagtttca 
        gtttttttc acccacttt ttctttatc ctgtggcca 
        tccttttaa
      • >TC005008 RD
        atggatgca agagtgaac gactttctt aacgaaatt 
        gacgaaata gccccactt agcgaaaac aaggaggat 
        tgtgcttgg gaaaagtgt tatgatgaa gagagcggt 
        tattattat tactggaac actaaaaca aacgaagta 
        acgtgggat atgcccgcc gaatacaaa tcattgaac 
        aaaaccaaa agtaaaaaa acttgcaac ttgtatatc 
        cctccacga acagccccc ttgtttcca agcacacgt 
        aacttgtta ccgcctgta tcagatgcc ataaaaatt 
        tataaaatt ggtgaatgt gctgggagt agtacaaag 
        agtgagaaa gttgtaaaa ggggaagaa cataaagtt 
        aaagaagag aaaaagact gtggtgaaa actaaaccg 
        gttaggaaa cacagtgat tctgatgat gaaaaaatt 
        gttttgatc tcatcttat agtggcgac agtgaatca 
        gaggaggaa aaggaggaa gcaaataca aaaaaattg 
        gtaagtcat acttttatt atctaa
      mRNA sequence
      • >TC005008 RA
        ttaaaacaa acaatatat ttgaagata aatggatca 
        aagccgctc cttacttca gaagataat gacgaagtt 
        acgaaacag aaaacatca actgaacct acgagtaaa 
        gcttcaatt gtaccgaaa aaacctgaa aacaaggaa 
        atttccggt ttttcactc gttgccggt tatagtgac 
        tccgatgaa gagcaggaa gaatcaaac cacaagcct 
        aatttttta ataggacca cctcctcaa gaaaataaa 
        gtttctcat agcaccctt tttcccgcc actaaacca 
        atagacgtt aaagatttt gctgaatcg ccaaaaata 
        atcggagaa ggtgatgaa ggttttgat tcaaaggcg 
        tttcaacgt aaacgccga attggagtt gcgttaata 
        aataccggt aaaaaaaca gtaattgat gatgataac 
        gaacgaaaa ggactggga ttttctaat gatagcaat 
        agtggtgag aattcaagc agtccgtat cccggattt 
        aaaagtgga ggtgttatg tttgtcaaa gctgatgtt 
        ttaaatcca acgagtaca gaagagaaa gctgaagat 
        tgtagtttg attgaagaa acgtacacg acattgaaa 
        gaaaaatta ggattttta agtgaaggt cgagaagag 
        gtttcacct gttcaagtg atgcttatt caagttgag 
        actctctac atcgcactc aagtcggga aatttaaaa 
        cacacctat ttaaagcaa tggcttgat gagaaatgc 
        tccgaactc attaaactt gaaaaagaa gcgactcca 
        gaaggttgg cttcttcaa tgggatagg tacgaaacg 
        tgcaatttt ttatttata gtttcagtt tttttcacc 
        cactttttc tttatcctg tggccatcc ttttaacct 
        tattattag ctccatttt ttctgctgt ttcccctcg 
        cagccaaca tcgagatat gcagtcaat tacttcagg 
        taagaatta ttttaattt tcatttaat tgatttgac 
        taaaaacga gctagaacg tatagtttt gacattgtt 
        gaaataaaa tcgaagatg cagttattc caattgtat 
        gaggcattt tttctgtag ttttaaatc aattcaggt 
        tcgtaaatt tattttcac ttgagttca ttacgtttt 
        gttcacttt tattaaagt ttatttatc acgtttaaa 
        tgagtggat ttaatgaaa tgaactcgt ttatccatt 
        taatagttt gattaattg caattgtat taatctttt 
        tttgtgtgt gtaggacat gaagattgg tggtattcc 
        tgtggtccg aacgcgaag ctggattta tttatctta 
        aatttctaa acttaaatt agtgtgtaa taataactg 
        ttttgcggt gtcgcaggt atagtatat gcagaaaag 
        tgaaaaaag taaaattgg aagagaaaa cttaaaaaa 
        agattcatc aatttcctt gtgtgttaa ttaacttga 
        tgaatctta tttatcatt tttccgcgc ctaaaaaat 
        cacttgacg acatcaagt caaaactgt gttctgtga 
        ggtggtttt ggtgatgtt ttaggtcac ataagcgct 
        atttttatc aaaatgcgt ctacggggg agagtcaat 
        gggaatatc cccaagccg atgttacag tgtgtgatg 
        aagctatgg atatttgta caacacctc ctccaacag 
        aaccaacaa ttcatatgt cgcctcctt tgcctccag 
        tcatcgcta gtccgagtc cgccgccac cacctagca 
        ttagtgact gcaagacca gtaaaaaaa agaaatgtg 
        atattcctc tccctcctg agccaaaaa aagatgtat 
        ctagtgaaa ggtatgcca gcaatgggg aaccatttc 
        ctccaggtg tggatgcgt ctgatgtga caacaaaaa 
        caaaggata ttaaaggaa atgatatga ccgatgttc 
        taaactcgt tttattcag atattgctg caattgaaa 
        ctagtaatt caccacctg tttctaatg ttaacgaag 
        atacaaaat tagagtcga ctgtggaca atgttcaag 
        aaccgatga agaaaaaga agaaaacga aagtgaaat 
        tggcacaag gaatagcta tgaagaaga aaggggtgt 
        cgaaattgg tggaaaaat ggaagaatg tacaattgt 
        catataatg attgaatgc aaaagtttt tttacctaa 
        ataataaag tagagatca aact
      • >TC005008 RB
        tgtcagatg gccgaacat aacctataa aaagttgat 
        tattttatt atttttgtg cataattta acaatgtgt 
        taaatgaaa atggatgca agagtgaac gactttctt 
        aacgaaatt gacgaaata gccccactt agcgaaaac 
        aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
        gagagcggt tattattat tactggaac actaaaaca 
        aacgaagta acgtgggat atgcccgcc gaatacaaa 
        tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
        ttgtatatc cctccacga acagccccc ttgtttcca 
        agcacacgt aacttgtta ccgcctgta tcagatgcc 
        ataaaaatt tataaaatt ggtgaatgt gctgggagt 
        agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
        cataaagtt aaagaagag aaaaagact gtggtgaaa 
        actaaaccg gttaggaaa cacagtgat tctgatgat 
        gaaaaaatt gttttgatc tcatcttat agtggcgac 
        agtgaatca gaggaggaa aaggaggaa gcaaataca 
        aaaaaattg gtaagcata tctttaaaa caaacaata 
        tatttgaag ataaatgga tcaaagccg ctccttact 
        tcagaagat aatgacgaa gttacgaaa cagaaaaca 
        tcaactgaa cctacgagt aaagcttca attgtaccg 
        aaaaaacct gaaaacaag gaaatttcc ggtttttca 
        ctcgttgcc ggttatagt gactccgat gaagagcag 
        gaagaatca aaccacaag cctaatttt ttaatagga 
        ccacctcct caagaaaat aaagtttct catagcacc 
        ctttttccc gccactaaa ccaatagac gttaaagat 
        tttgctgaa tcgccaaaa ataatcgga gaaggtgat 
        gaaggtttt gattcaaag gcgtttcaa cgtaaacgc 
        cgaattgga gttgcgtta ataaatacc ggtaaaaaa 
        acagtaatt gatgatgat aacgaacga aaaggactg 
        ggattttct aatgatagc aatagtggt gagaattca 
        agcagtccg tatcccgga tttaaaagt ggaggtgtt 
        atgtttgtc aaagctgat gttttaaat ccaacgagt 
        acagaagag aaagctgaa gattgtagt ttgattgaa 
        gaaacgtac acgacattg aaagaaaaa ttaggattt 
        ttaagtgaa ggtcgagaa gaggtttca cctgttcaa 
        gtgatgctt attcaagtt gagactctc tacatcgca 
        ctcaagtcg ggaaattta aaacacacc tatttaaag 
        caatggctt gatgagaaa tgctccgaa ctcattaaa 
        cttgaaaaa gaagcgact ccagaaggt tggcttctt 
        caatgggat aggtcacat aagcgctat ttttatcaa 
        aatgcgtct acgggggag agtcaatgg gaatatccc 
        caagccgat gttacagtg tgtgatgaa gctatggat 
        atttgtaca acacctcct ccaacagaa ccaacaatt 
        catatgtcg cctcctttg cctccagtc atcgctagt 
        ccgagtccg ccgccacca cctagcatt agtgactgc 
        aagaccagt aaaaaaaag aaatgtgat attcctctc 
        cctcctgag ccaaaaaaa gatgtatct agtgaaagg 
        tatgccagc aatggggaa ccatttcct ccaggtgtg 
        gatgcgtct gatgtgaca acaaaaaca aaggatatt 
        aaaggaaat gatatgacc gatgttcta aactcgttt 
        tattcagat attgctgca attgaaact agtaattca 
        ccacctgtt tctaatgtt aacgaagat acaaaatta 
        gagtcgact gtggacaat gttcaagaa ccgatgaag 
        aaaaagaag aaaacgaaa gtgaaattg gcacaagga 
        atagctatg aagaagaaa ggggtgtcg aaattggtg 
        gaaaaatgg aagaatgta caattgtca tataatgat 
        tgaatgcaa aagtttttt tacctaaat aataaagta 
        gagatcaaa ct
      • >TC005008 RC
        tgtcagatg gccgaacat aacctataa aaagttgat 
        tattttatt atttttgtg cataattta acaatgtgt 
        taaatgaaa atggatgca agagtgaac gactttctt 
        aacgaaatt gacgaaata gccccactt agcgaaaac 
        aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
        gagagcggt tattattat tactggaac actaaaaca 
        aacgaagta acgtgggat atgcccgcc gaatacaaa 
        tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
        ttgtatatc cctccacga acagccccc ttgtttcca 
        agcacacgt aacttgtta ccgcctgta tcagatgcc 
        ataaaaatt tataaaatt ggtgaatgt gctgggagt 
        agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
        cataaagtt aaagaagag aaaaagact gtggtgaaa 
        actaaaccg gttaggaaa cacagtgat tctgatgat 
        gaaaaaatt gttttgatc tcatcttat agtggcgac 
        agtgaatca gaggaggaa aaggaggaa gcaaataca 
        aaaaaattg gtaagcata tctttaaaa caaacaata 
        tatttgaag ataaatgga tcaaagccg ctccttact 
        tcagaagat aatgacgaa gttacgaaa cagaaaaca 
        tcaactgaa cctacgagt aaagcttca attgtaccg 
        aaaaaacct gaaaacaag gaaatttcc ggtttttca 
        ctcgttgcc ggttatagt gactccgat gaagagcag 
        gaagaatca aaccacaag cctaatttt ttaatagga 
        ccacctcct caagaaaat aaagtttct catagcacc 
        ctttttccc gccactaaa ccaatagac gttaaagat 
        tttgctgaa tcgccaaaa ataatcgga gaaggtgat 
        gaaggtttt gattcaaag gcgtttcaa cgtaaacgc 
        cgaattgga gttgcgtta ataaatacc ggtaaaaaa 
        acagtaatt gatgatgat aacgaacga aaaggactg 
        ggattttct aatgatagc aatagtggt gagaattca 
        agcagtccg tatcccgga tttaaaagt ggaggtgtt 
        atgtttgtc aaagctgat gttttaaat ccaacgagt 
        acagaagag aaagctgaa gattgtagt ttgattgaa 
        gaaacgtac acgacattg aaagaaaaa ttaggattt 
        ttaagtgaa ggtcgagaa gaggtttca cctgttcaa 
        gtgatgctt attcaagtt gagactctc tacatcgca 
        ctcaagtcg ggaaattta aaacacacc tatttaaag 
        caatggctt gatgagaaa tgctccgaa ctcattaaa 
        cttgaaaaa gaagcgact ccagaaggt tggcttctt 
        caatgggat aggtacgaa acgtgcaat tttttattt 
        atagtttca gtttttttc acccacttt ttctttatc 
        ctgtggcca tccttttaa ccttattat tagctccat 
        tttttctgc tgtttcccc tcgcagcca acatcgaga 
        tatgcagtc aattacttc aggtaagaa ttattttaa 
        ttttcattt aattgattt gactaaaaa cgagctaga 
        acgtatagt tttgacatt gttgaaata aaatcgaag 
        atgcagt
      • >TC005008 RD
        tgtcagatg gccgaacat aacctataa aaagttgat 
        tattttatt atttttgtg cataattta acaatgtgt 
        taaatgaaa atggatgca agagtgaac gactttctt 
        aacgaaatt gacgaaata gccccactt agcgaaaac 
        aaggaggat tgtgcttgg gaaaagtgt tatgatgaa 
        gagagcggt tattattat tactggaac actaaaaca 
        aacgaagta acgtgggat atgcccgcc gaatacaaa 
        tcattgaac aaaaccaaa agtaaaaaa acttgcaac 
        ttgtatatc cctccacga acagccccc ttgtttcca 
        agcacacgt aacttgtta ccgcctgta tcagatgcc 
        ataaaaatt tataaaatt ggtgaatgt gctgggagt 
        agtacaaag agtgagaaa gttgtaaaa ggggaagaa 
        cataaagtt aaagaagag aaaaagact gtggtgaaa 
        actaaaccg gttaggaaa cacagtgat tctgatgat 
        gaaaaaatt gttttgatc tcatcttat agtggcgac 
        agtgaatca gaggaggaa aaggaggaa gcaaataca 
        aaaaaattg gtaagtcat acttttatt atctaaaaa 
        ttagttttt gaaaaacaa attgaataa aataagcca 
        aaataa
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