Status
Please wait ...
Gene information
General information
TC002589 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 22623640..22626727
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002589
      iBeetle screen iB_10717

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002589 RA
        MDSHSHVLI SVIVPIYNG APWIDPCFK AVLQQELPD 
        DVNLEVCVC NDASDDGTV ALLHNWVDI FIKKKITLK 
        VFHNDLGKP RGVGYSKNR AVGISSGAY LCFQDIDDV 
        MLPNRIREQ YEKARNLPH DTIIGSQFK RVPENSTSR 
        YTQWANSLP DEKLHIQVY TSHGPTVIM PTWFCHRSV 
        FEKIGGFSE EGKGTPEDL IFFYKHLDL GGKVYRVNK 
        CLLVYTYHP SATTFSVME DTIWRLRLE RLENVVLRN 
        WQKFTIWNA GKQGRKLYN SLSEDSLKK VIALCDVDI 
        KKVGKTYTP FNSDTRKSG RSVDIVHFK NAEPPFVIC 
        VKINLTNGE FENNLSSLH LTENIDYVL FS
      CDS sequence
      • >TC002589 RA
        atggattca cattcacat gtgttaatt agtgttatt 
        gttcctatt tacaatggg gcaccatgg attgaccct 
        tgttttaaa gcagtttta caacaggag cttccagac 
        gacgtgaat ttggaagtg tgtgtctgt aacgatgcc 
        tcggacgat ggtacagtt gctttatta cacaattgg 
        gtcgatatt tttattaaa aaaaagatt acattaaaa 
        gtgtttcac aatgatttg ggaaagcca agaggcgtt 
        ggttattcc aaaaacagg gctgtcggc atcagcagt 
        ggtgcttat ttgtgtttt caagatata gatgatgtg 
        atgttacct aacaggata agagaacag tacgagaaa 
        gctagaaat ttacctcat gatacgatt attggatca 
        caattcaag cgagttcct gaaaactcc acaagtcgt 
        tacacacaa tgggcaaat tccctacca gatgaaaaa 
        cttcatatt caggtttat acatcacat ggtcctacc 
        gtaatcatg cccacttgg ttctgtcat cgctcagta 
        tttgaaaaa attggaggt ttttcagaa gaaggaaaa 
        ggtactcct gaagattta atatttttc tacaaacac 
        ttagattta ggaggaaaa gtgtacaga gtcaataag 
        tgcttattg gtctacacc taccatccc agtgcgact 
        acattttca gtcatggag gacacaatt tggaggctc 
        agactagag agactggaa aatgtggtt ctccgcaac 
        tggcagaaa tttacaatc tggaatgct ggtaaacaa 
        ggtcgaaag ttgtacaac tcattatca gaggactcg 
        ctgaaaaaa gtcatagct ttgtgcgac gttgatata 
        aagaaagtt ggcaaaact tatacacct tttaacagt 
        gatacaaga aagagtgga agatccgtc gatattgta 
        cattttaaa aacgctgaa ccgcctttt gtgatttgt 
        gtaaaaatt aatcttaca aacggagaa tttgaaaac 
        aacttatca tcattacac ttaacagaa aatattgat 
        tacgtttta tttagctga
      mRNA sequence
      • >TC002589 RA
        tgggtggcg ttcctagca gctcaccaa tggatttgg 
        cgggatgag attgatttt ttcttatat tattaatta 
        ttatgcaat gtataaaaa ttaaaaaaa ttatttgtt 
        gtaattgtg gggtttaaa gtggcacgt gacctgttg 
        tcagccaat cggaatgaa tgttttcat aacataacc 
        aaacaaatg tgacagctg tgttatttt cgtgttttt 
        ctgtgattt aatcatgga ttcacattc acatgtgtt 
        aattagtgt tattgttcc tatttacaa tggggcacc 
        atggattga cccttgttt taaagcagt tttacaaca 
        ggagcttcc agacgacgt gaatttgga agtgtgtgt 
        ctgtaacga tgcctcgga cgatggtac agttgcttt 
        attacacaa ttgggtcga tatttttat taaaaaaaa 
        gattacatt aaaagtgtt tcacaatga tttgggaaa 
        gccaagagg cgttggtta ttccaaaaa cagggctgt 
        cggcatcag cagtggtgc ttatttgtg ttttcaaga 
        tatagatga tgtgatgtt acctaacag gataagaga 
        acagtacga gaaagctag aaatttacc tcatgatac 
        gattattgg atcacaatt caagcgagt tcctgaaaa 
        ctccacaag tcgttacac acaatgggc aaattccct 
        accagatga aaaacttca tattcaggt ttatacatc 
        acatggtcc taccgtaat catgcccac ttggttctg 
        tcatcgctc agtatttga aaaaattgg aggtttttc 
        agaagaagg aaaaggtac tcctgaaga tttaatatt 
        tttctacaa acacttaga tttaggagg aaaagtgta 
        cagagtcaa taagtgctt attggtcta cacctacca 
        tcccagtgc gactacatt ttcagtcat ggaggacac 
        aatttggag gctcagact agagagact ggaaaatgt 
        ggttctccg caactggca gaaatttac aatctggaa 
        tgctggtaa acaaggtcg aaagttgta caactcatt 
        atcagagga ctcgctgaa aaaagtcat agctttgtg 
        cgacgttga tataaagaa agttggcaa aacttatac 
        accttttaa cagtgatac aagaaagag tggaagatc 
        cgtcgatat tgtacattt taaaaacgc tgaaccgcc 
        ttttgtgat ttgtgtaaa aattaatct tacaaacgg 
        agaatttga aaacaactt atcatcatt acacttaac 
        agaaaatat tgattacgt tttatttag ctgagtgaa 
        tgaaaatgt atttgtatt tgtactaag aaaaaataa 
        accttggat ttatta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY