Status
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Gene information
General information
TC033756
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009932,TC009933
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11683199..11698300
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033756
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10715

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033756 RA
      MTLYLLVHT ITITESDYV VQAQFFHTF TSHLLADAR 
      LIGRRMRYR PRVRGNRLK MHLQRLQTP DLRTIMKRK 
      TAKIRHHLR TIHLGSNHG LSKKEPSVE SAHLKTNNA 
      GAGPPQDKV DRKITEANV ISNDHLSSH STPEETTAW 
      LVNNRFDKY LETFANFSG ADMLRMSRD DLIQICGQA 
      DGIRFYNAI HLKTIAPKL KIYVCRQNT SIFNAVFLS 
      SHSNVELLE KLSELMGVS QDQVRDIYM EGPHSIHVQ 
      LNNDVIRHI KEETMFSVE ILQENGNFV FLLKRTVK
    • >TC033756 RB
      MDYELYDNS DILLNGDKL TNSKSSWNI PSDGSMDNN 
      DEYFDWNPL KIDEDHVEN GQATRQTRK RKLTAIEAS 
      PSKIKQSFK NAQDPLAID DKHQKKAQN GTVRMPTWQ 
      VEDITELNG DIDSSLSLG SDLANNSYN VSDAILSIS 
      GLTVFKQEA PSPTNNDVS QLQLVRQNR QSPTSTQQV 
      VHNGAIEET NNQNMINNT INQLLTQSA YTNLQNAID 
      ANLVSSPNS QDNYNVTST NYSFAEDCR FQYVLAAAT 
      SIATKVNED TLTYLNQGQ SYEIKLKKL GDLSMYRGK 
      LLKSVIRMC FHERRLQYM EKEQMAAWQ RARPGDRIL 
      EVDVPLSYG AFDIVQPTN ALNIIHFNW DPTKEVGVY 
      IKVNCISTE FTPKKHGGE KGVPFRIQV ETYQNGDNL 
      DSSVRLHAA ACQIKVFKL KGADRKHKQ DREKIMKRP 
      LSEQEKYQP SYECTVLND IPNDAVLNS PPIAPISAA 
      ESGSNHGLS KKEPSVESA HLKTNNAGA GPPQDKVDR 
      KITEANVIS NDHLSSHST PEETTAWLV NNRFDKYLE 
      TFANFSGAD MLRMSRDDL IQICGQADG IRFYNAIHL 
      KTIAPKLKI YVCRQNTSI FNAVFLSSH SNVELLEKL 
      SELMGVSQD QVRDIYMEG PHSIHVQLN NDVIRHIKE 
      ETMFSVEIL QENGNFVFL LKRTVK
    • >TC033756 RC
      MDYELYDNS DILLNGDKL TNSKSSWNI PSDGSMDNN 
      DEYFDWNPL KIDEDHVEN GQATRQTRK RKLTAIEAS 
      PSKIKQSFK NAQDPLAID DKHQKKAQN GTVRMPTWQ 
      VEDITELNG DIDSSLSLG SDLANNSYN VSDAILSIS 
      GLTVFKQEA PSPTNNDVS QLQLVRQNR QSPTSTQQV 
      VHNGAIEET NNQNMINNT INQLLTQSA YTNLQNAID 
      ANLVSSPNS QDNYNVTST NYSFAEDCR FQYVLAAAT 
      SIATKVNED TLTYLNQGQ SYEIKLKKL GDLSMYRGK 
      LLKSVIRMC FHERRLQYM EKEQMAAWQ RARPGDRIL 
      EVDVPLSYG AFDIVQPTN ALNIIHFNW DPTKEVGVY 
      IKVNCISTE FTPKKHGGE KGVPFRIQV ETYQNGDNL 
      DSSVRLHAA ACQIKVFKL KGADRKHKQ DREKIMKRP 
      LSEQEKYQP SYECTVLND IPNDAVLNS PPIAPISAA 
      ER
    CDS sequence
    • >TC033756 RA
      atgacgctc tacctcctg gtgcacacg atcacgatc 
      accgaaagt gattacgtc gtccaggcg cagtttttt 
      cacacgttt acgagccat ttgctggcc gatgccagg 
      ctcataggc cgcaggatg cgctacagg ccgagggtg 
      cgcggcaac aggctcaag atgcacctg cagaggctg 
      caaacgccc gacttgagg accatcatg aagaggaaa 
      acggccaaa atcagacac catctaagg accattcat 
      ctcgggagt aatcatggg ttgagcaag aaggagccg 
      agcgtcgag agtgcgcat ttgaagacg aataatgca 
      ggggctggt ccgccgcag gataaggtc gacaggaaa 
      atcaccgag gctaatgtc atttcgaat gaccatttg 
      tcgtcgcat tcgacgccg gaggaaacc acggcctgg 
      ttagtgaac aaccgtttc gacaaatat ctcgaaacc 
      tttgcaaac ttctcagga gctgacatg ttacgcatg 
      tcgagagac gacctcatt cagatttgt ggacaagct 
      gacggaatt cggttttat aacgcaatt catctaaaa 
      accatagcg ccgaagctg aaaatttac gtttgccgc 
      caaaacact tcgattttc aacgctgtt ttcctatcg 
      agtcacagc aatgtggag ttgttggag aagttgtcg 
      gagttgatg ggggtttcc caggatcag gtccgggac 
      atttacatg gaaggacca cattcaatt catgtgcaa 
      ttaaataac gatgttatt cgtcatatt aaggaagaa 
      acaatgttt tcggttgaa attctgcaa gaaaatggc 
      aatttcgtt tttcttttg aaacgtact gttaaataa
    • >TC033756 RB
      atggattac gaattgtat gacaactcg gacattttg 
      ctgaacggt gataaactc actaactcg aaaagttca 
      tggaacata cccagtgac ggtagcatg gataataac 
      gacgagtat ttcgactgg aatcctctc aaaatcgac 
      gaggatcat gtggagaac gggcaagcc accagacaa 
      acaaggaaa aggaaacta acagcaata gaagcgagt 
      ccttccaaa attaaacag agtttcaaa aatgcacaa 
      gatccgctc gcgatcgat gacaagcac cagaaaaag 
      gcgcaaaat ggcactgtc aggatgccc acgtggcaa 
      gtggaggat attacggaa ctgaacgga gacatcgac 
      agttctcta agtttgggg tccgacttg gccaacaat 
      tcctacaac gtcagtgat gccattctg tccatttcc 
      ggcttgacg gtctttaag caggaagcg ccttcgccg 
      acgaacaac gacgtgtcg cagctgcaa ctcgtccgc 
      cagaaccgc cagagcccc accagcacc cagcaagtg 
      gtccataat ggggccatc gaagagacc aacaaccag 
      aatatgatc aacaacacc atcaatcaa ttgctaact 
      cagtcggct tacacgaat ttgcaaaac gcgatcgat 
      gctaatctc gtctcttcg cccaactcg caggataac 
      tacaacgtg acttcgacc aattacagc ttcgcggag 
      gattgccgg tttcagtac gttttggcg gcggcgacc 
      agcatcgcc accaaagtc aacgaagac actttgacg 
      tatttgaat caggggcag agttacgag atcaagttg 
      aagaagttg ggcgatttg tcgatgtac cgggggaag 
      ttgttgaag tcggttatt aggatgtgc ttccatgaa 
      cgccggttg cagtatatg gaaaaggag caaatggcg 
      gcgtggcaa agggcgagg cctggtgat aggattttg 
      gaagttgat gtgcccttg tcgtatggc gcttttgat 
      attgtgcag ccgactaat gcgctaaat attattcat 
      ttcaattgg gatccaact aaggaagtt ggggtttat 
      attaaagtg aattgcata agcaccgaa ttcaccccg 
      aaaaagcac ggcggtgaa aagggcgtc ccgttccgc 
      atccaggtc gaaacttac caaaacggc gataatctc 
      gactcgtct gttcgtctt catgcagcg gcctgccaa 
      atcaaggtt ttcaagctg aaaggcgcc gacagaaag 
      cacaaacaa gatcgggaa aaaatcatg aaacggcct 
      ctatccgag caggaaaaa taccagccg agttacgaa 
      tgcaccgtt ttaaatgac attcccaac gatgccgtg 
      ctgaattcg cccccgata gcccccatt tctgccgct 
      gaaagcggg agtaatcat gggttgagc aagaaggag 
      ccgagcgtc gagagtgcg catttgaag acgaataat 
      gcaggggct ggtccgccg caggataag gtcgacagg 
      aaaatcacc gaggctaat gtcatttcg aatgaccat 
      ttgtcgtcg cattcgacg ccggaggaa accacggcc 
      tggttagtg aacaaccgt ttcgacaaa tatctcgaa 
      acctttgca aacttctca ggagctgac atgttacgc 
      atgtcgaga gacgacctc attcagatt tgtggacaa 
      gctgacgga attcggttt tataacgca attcatcta 
      aaaaccata gcgccgaag ctgaaaatt tacgtttgc 
      cgccaaaac acttcgatt ttcaacgct gttttccta 
      tcgagtcac agcaatgtg gagttgttg gagaagttg 
      tcggagttg atgggggtt tcccaggat caggtccgg 
      gacatttac atggaagga ccacattca attcatgtg 
      caattaaat aacgatgtt attcgtcat attaaggaa 
      gaaacaatg ttttcggtt gaaattctg caagaaaat 
      ggcaatttc gtttttctt ttgaaacgt actgttaaa 
      taa
    • >TC033756 RC
      atggattac gaattgtat gacaactcg gacattttg 
      ctgaacggt gataaactc actaactcg aaaagttca 
      tggaacata cccagtgac ggtagcatg gataataac 
      gacgagtat ttcgactgg aatcctctc aaaatcgac 
      gaggatcat gtggagaac gggcaagcc accagacaa 
      acaaggaaa aggaaacta acagcaata gaagcgagt 
      ccttccaaa attaaacag agtttcaaa aatgcacaa 
      gatccgctc gcgatcgat gacaagcac cagaaaaag 
      gcgcaaaat ggcactgtc aggatgccc acgtggcaa 
      gtggaggat attacggaa ctgaacgga gacatcgac 
      agttctcta agtttgggg tccgacttg gccaacaat 
      tcctacaac gtcagtgat gccattctg tccatttcc 
      ggcttgacg gtctttaag caggaagcg ccttcgccg 
      acgaacaac gacgtgtcg cagctgcaa ctcgtccgc 
      cagaaccgc cagagcccc accagcacc cagcaagtg 
      gtccataat ggggccatc gaagagacc aacaaccag 
      aatatgatc aacaacacc atcaatcaa ttgctaact 
      cagtcggct tacacgaat ttgcaaaac gcgatcgat 
      gctaatctc gtctcttcg cccaactcg caggataac 
      tacaacgtg acttcgacc aattacagc ttcgcggag 
      gattgccgg tttcagtac gttttggcg gcggcgacc 
      agcatcgcc accaaagtc aacgaagac actttgacg 
      tatttgaat caggggcag agttacgag atcaagttg 
      aagaagttg ggcgatttg tcgatgtac cgggggaag 
      ttgttgaag tcggttatt aggatgtgc ttccatgaa 
      cgccggttg cagtatatg gaaaaggag caaatggcg 
      gcgtggcaa agggcgagg cctggtgat aggattttg 
      gaagttgat gtgcccttg tcgtatggc gcttttgat 
      attgtgcag ccgactaat gcgctaaat attattcat 
      ttcaattgg gatccaact aaggaagtt ggggtttat 
      attaaagtg aattgcata agcaccgaa ttcaccccg 
      aaaaagcac ggcggtgaa aagggcgtc ccgttccgc 
      atccaggtc gaaacttac caaaacggc gataatctc 
      gactcgtct gttcgtctt catgcagcg gcctgccaa 
      atcaaggtt ttcaagctg aaaggcgcc gacagaaag 
      cacaaacaa gatcgggaa aaaatcatg aaacggcct 
      ctatccgag caggaaaaa taccagccg agttacgaa 
      tgcaccgtt ttaaatgac attcccaac gatgccgtg 
      ctgaattcg cccccgata gcccccatt tctgccgct 
      gaaaggtaa
    mRNA sequence
    • >TC033756 RA
      gggggttgc aaaccgaaa cctccaagg tgtttaaat 
      cgaacgtta tcggtagtc atcgccgta cgtgctcaa 
      aacttggct ccgcaccga attaaaccg ctcgttcct 
      ggctcaacc ggttccatc cagttccga gccatatct 
      agcatcaaa gtcgcctca aaccagacc tttcccgtc 
      gactgtgga atgcacatg tgtgcaaat acataatcg 
      aattaagcg aaataagtc cccgaacgt cagtggcgg 
      tgttcgctc gcgtattcc ggtgtcctg atgatgatt 
      tatcgctag ttcgatgga aaacaacat acgtttctg 
      ttgagcaaa aagcagttc gcgttcttg ccccagtgt 
      cggtccact tgcgaaact tatggttga ttttgcgcg 
      cgattcaac cgctcgatg cagtcagga atggttgaa 
      aaaaagccc aacgcccga gtcctcaaa accgccgaa 
      gtcgtctcc atggtcttg caccagctc gttagatga 
      caggaattt tcgcctagt ggtcagggc cgcaaaaag 
      aaaggatta cgtcctgaa gggcctgtg cttctgtca 
      cggcaggag ttcgctggc gctgatctg aaaccgcca 
      ccatgacgc tctacctcc tggtgcaca cgatcacga 
      tcaccgaaa gtgattacg tcgtccagg cgcagtttt 
      ttcacacgt ttacgagcc atttgctgg ccgatgcca 
      ggctcatag gccgcagga tgcgctaca ggccgaggg 
      tgcgcggca acaggctca agatgcacc tgcagaggc 
      tgcaaacgc ccgacttga ggaccatca tgaagagga 
      aaacggcca aaatcagac accatctaa ggaccattc 
      atctcggga gtaatcatg ggttgagca agaaggagc 
      cgagcgtcg agagtgcgc atttgaaga cgaataatg 
      caggggctg gtccgccgc aggataagg tcgacagga 
      aaatcaccg aggctaatg tcatttcga atgaccatt 
      tgtcgtcgc attcgacgc cggaggaaa ccacggcct 
      ggttagtga acaaccgtt tcgacaaat atctcgaaa 
      cctttgcaa acttctcag gagctgaca tgttacgca 
      tgtcgagag acgacctca ttcagattt gtggacaag 
      ctgacggaa ttcggtttt ataacgcaa ttcatctaa 
      aaaccatag cgccgaagc tgaaaattt acgtttgcc 
      gccaaaaca cttcgattt tcaacgctg ttttcctat 
      cgagtcaca gcaatgtgg agttgttgg agaagttgt 
      cggagttga tgggggttt cccaggatc aggtccggg 
      acatttaca tggaaggac cacattcaa ttcatgtgc 
      aattaaata acgatgtta ttcgtcata ttaaggaag 
      aaacaatgt tttcggttg aaattctgc aagaaaatg 
      gcaatttcg tttttcttt tgaaacgta ctgttaaat 
      aagagacaa agtggtgtg tctatattt gtgattctc 
      aatattact cagtgtctt atttgtatg ccttaagta 
      atttaggtc atattgcat ggttatagt gttatctca 
      ggtttaaat aaagcgtgc agcaagttt tattttaga 
      aatgtttta agtatttga gatttctat tatgtttac 
      atatttgta tcgttgttt agtttgtat ttatatttt 
      aagtgatgt tgcttattc tatatactt ttgaagttt 
      atttcgtta gagttgtta gttcgtaat aaggtatac 
      gtacatgtt cgcaaggaa aataaaatc aatattttt 
      tgatgtaca aattatatc gatagtttt tagccctag 
      ttataactt gtgtgtaaa ttagggaag gtagtaagc 
      atgaaataa ctttatttt taaatccat tacgtgcct 
      tgatatatt ctgcaacag agtgtaatc agagtgcca 
      taaagaaat atagccagc tttaagca
    • >TC033756 RB
      gtggataaa atttgaaaa cggggaaag cgggctcac 
      tttgtaaaa ttcagagat tcaagaagg agtctctcg 
      tctcatgac gagtagttt ttgttgtat tttcggtcc 
      aatttaggt gtgagtatt gtctaaaat ctgaatgca 
      ttcacggct tgatccaaa tccttcgat tgttttgtt 
      gaaaccgtc gtctgctga tcccttcaa gctaccgag 
      aaggttcgg cttctggat accgaaaag ggagaaaaa 
      gaggggttt gatttaagc gactttccg agaaatact 
      cgtcgttag ccaaatcga aatctcgac gttaaagag 
      cgcacaacc aactcgaca aagaaggga caattaata 
      agaaaaaaa tagtttgct atttgttgt gtaaattga 
      gctcaattt aagtaattc aacaattga tggggcaag 
      cagttgttt aaacaaggt ctcgtcagt tcgaccacg 
      atagtcttg taaggcgtt accgctgag acgtccacc 
      aatcgtaca gtgaggaaa caattcgca agtgtatga 
      gaaccggat gaataaaat tttgaatga cgaacgttt 
      accgagagt gatccaatt cgccaaaac ggcttgcaa 
      gcgaacact aacttgaga gttggttgt gtgtcggtg 
      gagtgcgcc cttgagcag aagggtttg actgtttaa 
      ccgcaaaac ccgtgtgat aaatggata ttgaatcgt 
      atcatttgc aaaataaaa tgttaacaa tcgataata 
      gcttgctcc aaaatcacc gtcagcgag aaacgaccg 
      gaatcaaga aattgtaca attccttct ttgttatgt 
      tctaaaaat cgcgaggac tcgattaat ccaggactc 
      agttcggtc gaacgaaag tagctagat tcgattttt 
      ttatccgga gattccggc ccgcggcag tttttgtag 
      tatggatta cgaattgta tgacaactc ggacatttt 
      gctgaacgg tgataaact cactaactc gaaaagttc 
      atggaacat acccagtga cggtagcat ggataataa 
      cgacgagta tttcgactg gaatcctct caaaatcga 
      cgaggatca tgtggagaa cgggcaagc caccagaca 
      aacaaggaa aaggaaact aacagcaat agaagcgag 
      tccttccaa aattaaaca gagtttcaa aaatgcaca 
      agatccgct cgcgatcga tgacaagca ccagaaaaa 
      ggcgcaaaa tggcactgt caggatgcc cacgtggca 
      agtggagga tattacgga actgaacgg agacatcga 
      cagttctct aagtttggg gtccgactt ggccaacaa 
      ttcctacaa cgtcagtga tgccattct gtccatttc 
      cggcttgac ggtctttaa gcaggaagc gccttcgcc 
      gacgaacaa cgacgtgtc gcagctgca actcgtccg 
      ccagaaccg ccagagccc caccagcac ccagcaagt 
      ggtccataa tggggccat cgaagagac caacaacca 
      gaatatgat caacaacac catcaatca attgctaac 
      tcagtcggc ttacacgaa tttgcaaaa cgcgatcga 
      tgctaatct cgtctcttc gcccaactc gcaggataa 
      ctacaacgt gacttcgac caattacag cttcgcgga 
      ggattgccg gtttcagta cgttttggc ggcggcgac 
      cagcatcgc caccaaagt caacgaaga cactttgac 
      gtatttgaa tcaggggca gagttacga gatcaagtt 
      gaagaagtt gggcgattt gtcgatgta ccgggggaa 
      gttgttgaa gtcggttat taggatgtg cttccatga 
      acgccggtt gcagtatat ggaaaagga gcaaatggc 
      ggcgtggca aagggcgag gcctggtga taggatttt 
      ggaagttga tgtgccctt gtcgtatgg cgcttttga 
      tattgtgca gccgactaa tgcgctaaa tattattca 
      tttcaattg ggatccaac taaggaagt tggggttta 
      tattaaagt gaattgcat aagcaccga attcacccc 
      gaaaaagca cggcggtga aaagggcgt cccgttccg 
      catccaggt cgaaactta ccaaaacgg cgataatct 
      cgactcgtc tgttcgtct tcatgcagc ggcctgcca 
      aatcaaggt tttcaagct gaaaggcgc cgacagaaa 
      gcacaaaca agatcggga aaaaatcat gaaacggcc 
      tctatccga gcaggaaaa ataccagcc gagttacga 
      atgcaccgt tttaaatga cattcccaa cgatgccgt 
      gctgaattc gcccccgat agcccccat ttctgccgc 
      tgaaagcgg gagtaatca tgggttgag caagaagga 
      gccgagcgt cgagagtgc gcatttgaa gacgaataa 
      tgcaggggc tggtccgcc gcaggataa ggtcgacag 
      gaaaatcac cgaggctaa tgtcatttc gaatgacca 
      tttgtcgtc gcattcgac gccggagga aaccacggc 
      ctggttagt gaacaaccg tttcgacaa atatctcga 
      aacctttgc aaacttctc aggagctga catgttacg 
      catgtcgag agacgacct cattcagat ttgtggaca 
      agctgacgg aattcggtt ttataacgc aattcatct 
      aaaaaccat agcgccgaa gctgaaaat ttacgtttg 
      ccgccaaaa cacttcgat tttcaacgc tgttttcct 
      atcgagtca cagcaatgt ggagttgtt ggagaagtt 
      gtcggagtt gatgggggt ttcccagga tcaggtccg 
      ggacattta catggaagg accacattc aattcatgt 
      gcaattaaa taacgatgt tattcgtca tattaagga 
      agaaacaat gttttcggt tgaaattct gcaagaaaa 
      tggcaattt cgtttttct tttgaaacg tactgttaa 
      ataagagac aaagtggtg tgtctatat ttgtgattc 
      tcaatatta ctcagtgtc ttatttgta tgccttaag 
      taatttagg tcatattgc atggttata gtgttatct 
      caggtttaa ataaagcgt gcagcaagt tttatttta 
      gaaatgttt taagtattt gagatttct attatgttt 
      acatatttg tatcgttgt ttagtttgt atttatatt 
      ttaagtgat gttgcttat tctatatac ttttgaagt 
      ttatttcgt tagagttgt tagttcgta ataaggtat 
      acgtacatg ttcgcaagg aaaataaaa tcaatattt 
      tttgatgta caaattata tcgatagtt tttagccct 
      agttataac ttgtgtgta aattaggga aggtagtaa 
      gcatgaaat aactttatt tttaaatcc attacgtgc 
      cttgatata ttctgcaac agagtgtaa tcagagtgc 
      cataaagaa atatagcca gctttaagc a
    • >TC033756 RC
      gtggataaa atttgaaaa cggggaaag cgggctcac 
      tttgtaaaa ttcagagat tcaagaagg agtctctcg 
      tctcatgac gagtagttt ttgttgtat tttcggtcc 
      aatttaggt gtgagtatt gtctaaaat ctgaatgca 
      ttcacggct tgatccaaa tccttcgat tgttttgtt 
      gaaaccgtc gtctgctga tcccttcaa gctaccgag 
      aaggttcgg cttctggat accgaaaag ggagaaaaa 
      gaggggttt gatttaagc gactttccg agaaatact 
      cgtcgttag ccaaatcga aatctcgac gttaaagag 
      cgcacaacc aactcgaca aagaaggga caattaata 
      agaaaaaaa tagtttgct atttgttgt gtaaattga 
      gctcaattt aagtaattc aacaattga tggggcaag 
      cagttgttt aaacaaggt ctcgtcagt tcgaccacg 
      atagtcttg taaggcgtt accgctgag acgtccacc 
      aatcgtaca gtgaggaaa caattcgca agtgtatga 
      gaaccggat gaataaaat tttgaatga cgaacgttt 
      accgagagt gatccaatt cgccaaaac ggcttgcaa 
      gcgaacact aacttgaga gttggttgt gtgtcggtg 
      gagtgcgcc cttgagcag aagggtttg actgtttaa 
      ccgcaaaac ccgtgtgat aaatggata ttgaatcgt 
      atcatttgc aaaataaaa tgttaacaa tcgataata 
      gcttgctcc aaaatcacc gtcagcgag aaacgaccg 
      gaatcaaga aattgtaca attccttct ttgttatgt 
      tctaaaaat cgcgaggac tcgattaat ccaggactc 
      agttcggtc gaacgaaag tagctagat tcgattttt 
      ttatccgga gattccggc ccgcggcag tttttgtag 
      tatggatta cgaattgta tgacaactc ggacatttt 
      gctgaacgg tgataaact cactaactc gaaaagttc 
      atggaacat acccagtga cggtagcat ggataataa 
      cgacgagta tttcgactg gaatcctct caaaatcga 
      cgaggatca tgtggagaa cgggcaagc caccagaca 
      aacaaggaa aaggaaact aacagcaat agaagcgag 
      tccttccaa aattaaaca gagtttcaa aaatgcaca 
      agatccgct cgcgatcga tgacaagca ccagaaaaa 
      ggcgcaaaa tggcactgt caggatgcc cacgtggca 
      agtggagga tattacgga actgaacgg agacatcga 
      cagttctct aagtttggg gtccgactt ggccaacaa 
      ttcctacaa cgtcagtga tgccattct gtccatttc 
      cggcttgac ggtctttaa gcaggaagc gccttcgcc 
      gacgaacaa cgacgtgtc gcagctgca actcgtccg 
      ccagaaccg ccagagccc caccagcac ccagcaagt 
      ggtccataa tggggccat cgaagagac caacaacca 
      gaatatgat caacaacac catcaatca attgctaac 
      tcagtcggc ttacacgaa tttgcaaaa cgcgatcga 
      tgctaatct cgtctcttc gcccaactc gcaggataa 
      ctacaacgt gacttcgac caattacag cttcgcgga 
      ggattgccg gtttcagta cgttttggc ggcggcgac 
      cagcatcgc caccaaagt caacgaaga cactttgac 
      gtatttgaa tcaggggca gagttacga gatcaagtt 
      gaagaagtt gggcgattt gtcgatgta ccgggggaa 
      gttgttgaa gtcggttat taggatgtg cttccatga 
      acgccggtt gcagtatat ggaaaagga gcaaatggc 
      ggcgtggca aagggcgag gcctggtga taggatttt 
      ggaagttga tgtgccctt gtcgtatgg cgcttttga 
      tattgtgca gccgactaa tgcgctaaa tattattca 
      tttcaattg ggatccaac taaggaagt tggggttta 
      tattaaagt gaattgcat aagcaccga attcacccc 
      gaaaaagca cggcggtga aaagggcgt cccgttccg 
      catccaggt cgaaactta ccaaaacgg cgataatct 
      cgactcgtc tgttcgtct tcatgcagc ggcctgcca 
      aatcaaggt tttcaagct gaaaggcgc cgacagaaa 
      gcacaaaca agatcggga aaaaatcat gaaacggcc 
      tctatccga gcaggaaaa ataccagcc gagttacga 
      atgcaccgt tttaaatga cattcccaa cgatgccgt 
      gctgaattc gcccccgat agcccccat ttctgccgc 
      tgaaaggta aatatatgc ttgatattt tgctactct 
      acttcattt ccctaatta agtgcaagg atcggttaa 
      agaaacagg taaaaatga a
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