Status
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Gene information
General information
TC002178 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 852596..866112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002178
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10712

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs potentiallysize shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen posterior potentiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen posterior ventralconstricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002178 RA
        MSFNLENDC SAVATVTGI VRNSRQAVI ISEKLLLDK 
        QLSNKIKWW RACDLEEVG VEFLVFKSA FSSSSRFVV 
        FLRKNKPAG EEFSKDAKR TGIGYLALS LGFCFRVIA 
        FSRSFRCCF ELLNRFMAP KSIKKCIAL RETAITQMN 
        ELKKAFMNE YLDLGHMQL LTNGNSSDG YYIPHHCVI 
        KADSPSTKL RVVFDASCP SSEGKSLND SLFVGPKLQ 
        KDILTILSR FRVHPIVFT AHIRQMYRQ ILILPEHRK 
        FQKILWRNF PQEPVQEYR LNTVTYGVS FSPFLAIRT 
        LGELASLEK TQFPLASEV LANDVYIDD IITGCSSIA 
        EAHNLRDEL IDLFSTAGF ELRKWSSND PTVLNKNPL 
        SHQLDQALA FNSESEFFV KVLGLQWIP RSFSYSIQP 
        FNRPCSKRS LLSELARIF DSIGFLAPL TFYAKRLIQ 
        YLWTLKLSW DDSPPDDII SSWTQFCSE LPQLATLQI 
        PRKIIVEKV RLCTLHGFC DASESGYAA VDLRSVSVT 
        HAIEIRLIC AKSKVAPLK KISIPRLEL CAAVLLAKL 
        IDYTLTTFK GVIEIEQVY AWSDSTITL SWIQSPPHL 
        WKVFVSNRV ALIQSLAPT ATWHHVSSD DNPADCASR 
        GLSPMHLLQ HLLWWAGLT WLCNFDLVV GKSEIPDLE 
        NDSIIVEEQ RKIKVLQTE ESFFDRILS TFSSLRKIL 
        RIIAVWLRF VKNFKTKTR NFEALSEDE INYALTLLV 
        KHVQQVFAS EVKNLESCF KPFRKLALF VDPQGILRV 
        GGRLKHSGL SFDAKHPML LPRGHRLTE LIVEDMHIR 
        YFHPGTQTL HFLLSQRFW ILGAKKLIS KIISRCLRC 
        WRLNPRSPQ PPMGDLPSL RISQIKPFS CVGVDYGGS 
        FKITLGEQI LTFEELYTV LTQIESVLN SRPLCPVSA 
        DPNDFSVLT PGHFLTLEP FSASPDSDL SSLKINRLT 
        RWQLVQRLH QDFWSRWHQ EYLHTLQQR SKWNTSSKS 
        IVPDTLVIV KNECLSPLR WQLGRIVAV HPGTDGVVR 
        VATVRTSQE VGVEFLVFK SASSSSSRF VVFLRKNKP 
        AGEEFSKDA KRGSFTKLD NVPISHRRP ASVVNKLIH 
        EKVAS
      CDS sequence
      • >TC002178 RA
        atgagtttt aacttggaa aacgattgc tcagcagta 
        gcgactgtt acaggaatt gttaggaat agcaggcaa 
        gctgtaata atttctgag aaactactg ctggataaa 
        caattgtct aataaaata aagtggtgg cgcgcgtgt 
        gaccttgaa gaagttggt gttgagttc ttggttttt 
        aaaagcgca tttagtagt tcgtcccgc ttcgtggtt 
        tttctccga aagaacaag ccggccggc gaagaattt 
        tccaaagac gccaaaaga acaggtatt ggttatctc 
        gctttatcg ctgggtttt tgctttcgg gttatcgct 
        ttttctcgc tcttttcgt tgttgtttc gaattgtta 
        aatcgattt atggcccct aaatccata aaaaaatgt 
        attgctctt cgggaaacc gctattacg caaatgaac 
        gagttaaaa aaggctttt atgaacgaa tatttagat 
        cttggccac atgcagctc cttacaaat ggaaattcg 
        tcggatgga tattatatt ccccaccac tgtgttata 
        aaggctgat agtccaagc acaaaactt cgcgtagtt 
        ttcgatgcc tcctgtcct tcgtcagaa ggcaaatct 
        ctcaatgat agtttattt gtaggtcca aaacttcaa 
        aaggacatt ttgactatt ctttcacgc tttagagtt 
        catcccatc gtgttcacc gcacatata cgacagatg 
        tatcgtcaa attttgatt cttcctgaa catcgcaaa 
        tttcagaaa attttgtgg agaaacttc ccacaggaa 
        cccgttcag gaatatcgg ttaaatacc gttacctac 
        ggagtgtcg ttttctccc tttttggca attcgcaca 
        ttaggcgaa cttgcatct ctggaaaaa actcaattt 
        cctctcgct tccgaagtc ctggctaac gatgtttat 
        atcgatgac attattaca ggttgctct tcgattgct 
        gaagctcat aatttgcgg gatgaacta attgacttg 
        ttcagtact gcaggtttc gaattaaga aagtggtct 
        agcaatgac cctaccgtt ttaaacaaa aatcctctt 
        tcacatcaa ttagatcaa gctcttgcg ttcaatagt 
        gaatcagag tttttcgtt aaagttttg ggactgcaa 
        tggatcccg cgtagtttt tcttatagt attcaaccg 
        ttcaatcgt ccctgctca aaacgatcg cttttgtct 
        gaattggct cgcatattc gactctatt gggtttttg 
        gctccttta actttttac gcaaaacgt ttaattcag 
        tatctatgg actttgaag ttgtcttgg gatgactcg 
        cctcctgac gacattatt tcgagttgg acgcaattt 
        tgttcggaa cttccccaa ttagcgact ttgcaaatt 
        cccagaaaa attatcgta gaaaaagtt cggttgtgt 
        acccttcat ggtttctgc gacgctagt gagtctggt 
        tacgccgca gtggattta cgaagtgtc tcagttacc 
        catgcaata gaaattcgt ctcatttgt gcaaagtca 
        aaggtagcg ccgttgaag aaaatctcc attccgcgt 
        ctagagtta tgtgcggca gttttattg gccaagtta 
        atagattac acccttacc acgtttaaa ggtgtcata 
        gagattgaa caagtttac gcatggtcc gactctacc 
        ataaccctt tcgtggatt caatcgcct cctcacctt 
        tggaaggta tttgttagc aatagggtt gctcttatt 
        caatcgtta gcacccact gctacttgg catcacgtt 
        tcgtcggat gacaacccc gccgattgc gcgtctcgt 
        ggtttaagt ccaatgcat ctcctgcag cacctgcta 
        tggtgggcg gggctcact tggttatgc aattttgat 
        ttggtcgta ggtaaatcg gagatacca gatttggaa 
        aatgactcg ataatagta gaagaacaa cgcaagatt 
        aaggttctt caaacagaa gaaagtttt tttgatcgc 
        atattgagt accttctct tctcttcga aaaatatta 
        cgcattatc gcagtgtgg ctacggttt gtcaaaaat 
        tttaaaact aaaactcga aattttgaa gccttaagt 
        gaggatgaa ataaactac gcattgacc ctgctcgtt 
        aaacatgtc caacaagta ttcgcctcg gaagttaaa 
        aatctggaa tcttgtttc aaacccttc agaaaattg 
        gcgctcttt gtagatcct caagggatt ttacgtgta 
        ggtggtcgc ctcaaacat tctggtctt tctttcgat 
        gctaaacat ccgatgtta cttcctcgt ggacatcgt 
        ttaaccgag ctaattgtt gaggatatg cacattcgg 
        tattttcac cccggcact caaaccctc catttccta 
        ttgtctcag agattttgg atattgggg gctaaaaaa 
        cttatttcg aaaataatt tctcgttgt ttacgctgt 
        tggcgtttg aaccctcgt tctcctcaa cctccaatg 
        ggagattta ccgtcgctg cgtatttct caaatcaag 
        cctttttcg tgtgttgga gtggattat ggtggttct 
        tttaagatc actttagga gagcaaatc ctcacattc 
        gaagaattg tatacggtt ctaactcaa attgagtca 
        gtacttaat tcgcgtcct ctttgcccg gttagcgcc 
        gatcctaac gacttctca gttttaacc cctggccat 
        ttcttgacg ttagaacct tttagcgca tctcctgat 
        tcagactta tcgtcactg aaaataaat cgattaacg 
        cgctggcaa ttagtccaa cgtctgcac caagatttc 
        tggtcacga tggcatcag gaatattta cacactctt 
        cagcaacga agtaagtgg aatacttct tcaaaatca 
        atcgttcct gatacttta gtgattgtt aaaaatgaa 
        tgcctatct cctttacga tggcagtta ggaagaatc 
        gtcgcggtg catcctggt acagatggg gtcgtcaga 
        gttgccaca gtacgtacc tcccaggaa gttggtgtt 
        gagttcttg gtttttaaa agcgcatct agtagttcg 
        tcccgcttc gtggttttt ctccgaaag aacaagccg 
        gccggcgaa gaattttcc aaagacgcc aaaaggggg 
        tcattcacg aaactcgat aacgttccg ataagtcat 
        cgacgacct gcaagcgtc gttaataaa ctgattcac 
        gaaaaagtt gcaagttag
      mRNA sequence
      • >TC002178 RA
        atgagtttt aacttggaa aacgattgc tcagcagta 
        gcgactgtt acaggaatt gttaggaat agcaggcaa 
        gctgtaata atttctgag aaactactg ctggataaa 
        caattgtct aataaaata aagtggtgg cgcgcgtgt 
        gaccttgaa gaagttggt gttgagttc ttggttttt 
        aaaagcgca tttagtagt tcgtcccgc ttcgtggtt 
        tttctccga aagaacaag ccggccggc gaagaattt 
        tccaaagac gccaaaaga acaggtatt ggttatctc 
        gctttatcg ctgggtttt tgctttcgg gttatcgct 
        ttttctcgc tcttttcgt tgttgtttc gaattgtta 
        aatcgattt atggcccct aaatccata aaaaaatgt 
        attgctctt cgggaaacc gctattacg caaatgaac 
        gagttaaaa aaggctttt atgaacgaa tatttagat 
        cttggccac atgcagctc cttacaaat ggaaattcg 
        tcggatgga tattatatt ccccaccac tgtgttata 
        aaggctgat agtccaagc acaaaactt cgcgtagtt 
        ttcgatgcc tcctgtcct tcgtcagaa ggcaaatct 
        ctcaatgat agtttattt gtaggtcca aaacttcaa 
        aaggacatt ttgactatt ctttcacgc tttagagtt 
        catcccatc gtgttcacc gcacatata cgacagatg 
        tatcgtcaa attttgatt cttcctgaa catcgcaaa 
        tttcagaaa attttgtgg agaaacttc ccacaggaa 
        cccgttcag gaatatcgg ttaaatacc gttacctac 
        ggagtgtcg ttttctccc tttttggca attcgcaca 
        ttaggcgaa cttgcatct ctggaaaaa actcaattt 
        cctctcgct tccgaagtc ctggctaac gatgtttat 
        atcgatgac attattaca ggttgctct tcgattgct 
        gaagctcat aatttgcgg gatgaacta attgacttg 
        ttcagtact gcaggtttc gaattaaga aagtggtct 
        agcaatgac cctaccgtt ttaaacaaa aatcctctt 
        tcacatcaa ttagatcaa gctcttgcg ttcaatagt 
        gaatcagag tttttcgtt aaagttttg ggactgcaa 
        tggatcccg cgtagtttt tcttatagt attcaaccg 
        ttcaatcgt ccctgctca aaacgatcg cttttgtct 
        gaattggct cgcatattc gactctatt gggtttttg 
        gctccttta actttttac gcaaaacgt ttaattcag 
        tatctatgg actttgaag ttgtcttgg gatgactcg 
        cctcctgac gacattatt tcgagttgg acgcaattt 
        tgttcggaa cttccccaa ttagcgact ttgcaaatt 
        cccagaaaa attatcgta gaaaaagtt cggttgtgt 
        acccttcat ggtttctgc gacgctagt gagtctggt 
        tacgccgca gtggattta cgaagtgtc tcagttacc 
        catgcaata gaaattcgt ctcatttgt gcaaagtca 
        aaggtagcg ccgttgaag aaaatctcc attccgcgt 
        ctagagtta tgtgcggca gttttattg gccaagtta 
        atagattac acccttacc acgtttaaa ggtgtcata 
        gagattgaa caagtttac gcatggtcc gactctacc 
        ataaccctt tcgtggatt caatcgcct cctcacctt 
        tggaaggta tttgttagc aatagggtt gctcttatt 
        caatcgtta gcacccact gctacttgg catcacgtt 
        tcgtcggat gacaacccc gccgattgc gcgtctcgt 
        ggtttaagt ccaatgcat ctcctgcag cacctgcta 
        tggtgggcg gggctcact tggttatgc aattttgat 
        ttggtcgta ggtaaatcg gagatacca gatttggaa 
        aatgactcg ataatagta gaagaacaa cgcaagatt 
        aaggttctt caaacagaa gaaagtttt tttgatcgc 
        atattgagt accttctct tctcttcga aaaatatta 
        cgcattatc gcagtgtgg ctacggttt gtcaaaaat 
        tttaaaact aaaactcga aattttgaa gccttaagt 
        gaggatgaa ataaactac gcattgacc ctgctcgtt 
        aaacatgtc caacaagta ttcgcctcg gaagttaaa 
        aatctggaa tcttgtttc aaacccttc agaaaattg 
        gcgctcttt gtagatcct caagggatt ttacgtgta 
        ggtggtcgc ctcaaacat tctggtctt tctttcgat 
        gctaaacat ccgatgtta cttcctcgt ggacatcgt 
        ttaaccgag ctaattgtt gaggatatg cacattcgg 
        tattttcac cccggcact caaaccctc catttccta 
        ttgtctcag agattttgg atattgggg gctaaaaaa 
        cttatttcg aaaataatt tctcgttgt ttacgctgt 
        tggcgtttg aaccctcgt tctcctcaa cctccaatg 
        ggagattta ccgtcgctg cgtatttct caaatcaag 
        cctttttcg tgtgttgga gtggattat ggtggttct 
        tttaagatc actttagga gagcaaatc ctcacattc 
        gaagaattg tatacggtt ctaactcaa attgagtca 
        gtacttaat tcgcgtcct ctttgcccg gttagcgcc 
        gatcctaac gacttctca gttttaacc cctggccat 
        ttcttgacg ttagaacct tttagcgca tctcctgat 
        tcagactta tcgtcactg aaaataaat cgattaacg 
        cgctggcaa ttagtccaa cgtctgcac caagatttc 
        tggtcacga tggcatcag gaatattta cacactctt 
        cagcaacga agtaagtgg aatacttct tcaaaatca 
        atcgttcct gatacttta gtgattgtt aaaaatgaa 
        tgcctatct cctttacga tggcagtta ggaagaatc 
        gtcgcggtg catcctggt acagatggg gtcgtcaga 
        gttgccaca gtacgtacc tcccaggaa gttggtgtt 
        gagttcttg gtttttaaa agcgcatct agtagttcg 
        tcccgcttc gtggttttt ctccgaaag aacaagccg 
        gccggcgaa gaattttcc aaagacgcc aaaaggggg 
        tcattcacg aaactcgat aacgttccg ataagtcat 
        cgacgacct gcaagcgtc gttaataaa ctgattcac 
        gaaaaagtt gcaagttag
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY