| General information | |||
| TC013292 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 6384504..6394528 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC013292 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
For help on how to submit larger datasets or non experimental data please contact us.
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10709 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC013292 RA
MKFSKEDHI CFIKLFYDL LTIPDLEPT RINKCASTL TQLLKKKYL ISRDELQLE WRPLYDLCI RVMEKSKTD IGMYRCFST LEGTLMNVI RLARIYFPA SATQEILDE FRPRLCPMS SPTILSAIQ YLEIFLPVC VKPEEAAIS YDLWFEELM TLWNACHNA SAWEDHMMW MIARLSSYT MGYINWEPY IPMMFVRFS RSFQLPVAY RQKQTGKQY KIEISAMAI WITCALNGD KNSTFFHLE KFMQALESY YYPANMGRW SMKLRELLR KLAYYFVQR VHCERYKKK TWDTNIPDH YKLTDEDID RFVNIMKPC LEQAMFSRQ GGQDIILAL QYLGSLRPN LVIPMTLDK LYASMDSLT EPHKLTSSM MGVTAVARY MVQGARSNY PEGFTHVLP LLMALLPGI DPNDIRKCL VTFNFMVHF INMIPLINC SEASKYHDD LTEEEHIVC EASDGFEDF VLQFFDRLC LWVESNSLD FVRLEQDAN SNNNKNRAE VIAETALFS IISTVLHQC SPEIFTAAL KKVFNFVSD KVMEIQISG KMTAIICQG FAKVNPKET LKLFIPHLC DIIEGLMNE NPNIEKEEH LDAELLYNL QILSEVVDA RTELVTYSD RLMKILDRT LHMSSLSGS QMASRMLEV MMTSLTYIL PTEYRSCST PYDTHVKDF LSIREWGKA QNIKDLEIS WYVPGDKEI ALVQSLINK YLVPEVSKL SKHASGESV LTREELRRS LRIVISILS SHPVLPIWD EPAIQLVDS VLDPWAFNL IVSDSHAVN MPDGSNIRK VIADLMHKV QKKILEVDE GDTKSIHNI VHIYNILLF NKSRVHDFE YQWKNFHLV KKLLEDRLH QNKLHLRHI LINRVMLQQ EFRIESRSC SFTETHKQI LLDLFELSV SRYSEVRIS AQSKLFSVV SYFPYSYTV LTSRIFEIL KLNSEEHHE KFKGCLYIL ISPKNTPIV ARHDWNFIR QLWPLIIKS MPSEKPSIV NLINAIVDA VNRFFPTIA INLVFPQRC LDDATLLSQ NVPKCDLTH FQKYMDEGE KTLQIKCQK RAVAYNETV DGLLDACIN GNLHWRYHS MAVTFLKVL VHYDVKYNV RIVKYFLQA LINESITIR KTALRVSLF ILIQNKPKF TKIEIDPYG FSNSKRNNH KLRPGIRED NKWLLYDSK TVPKDSEAW EKPRFLHDH SIGYYAWPT KVEIYAPPS EQPHIDKRF PKLSEEEKE IYEFFSNAE NLDQFIKYF SMEEKKGKD QFNAYKFLL FKNLFKLFE DKLLDPIIP HLEKLVADK NESSQRCAA EIISGLIRG SKHWSFDKI TKLWSVLIP ILETAVVNM CSETMTDWG LSMAMALDS RDPNRYHWI LEYLVKDPL SDQTSFVAC GRLHMLHAA LNQQTWRNN ELSVRLFDY FKQHLSHPF QNIRDKISS CLTMIFSKD LIFPDDAND TNSAGIKAF FKEIMPTLD NLYSYTLSK IESKDNPMD TSEVSKDMQ GLSLENDEV RENAMRLFK LVSKYVTSS VVRMNYSTL PEYFEILPL SCVLQSNDT DDEIAPICS SLLAVLAYT ITIDKYMPS ALEAIRKVA TCPFWSARA VITEFMSGF VFHNMPTII SKKEWVLDI QHMVLELLE DVQPEVRVS ASQVLSGLL HCQFIPNPQ ELLAQFKQK AKTKLKKKN LNNSNSLNL STLRFRHAG VLGLCAFIN SHPYDVPDF LPDVFGQLG PHLNDPQPI PATIRKTLG DFKRTHHEN WEIHKLKFT EEELLVLSD LTVPPSYYA
- >TC013292 RB
MDENVSKRR YEKLGFKPQ KENVYNKLL PYAEKLDEE STQLYADIK TNLIKSVLA REMRPGCAL WTSRLNKYI KIYGMKFSK EDHICFIKL FYDLLTIPD LEPTRINKC ASTLTQLLK KKYLISRDE LQLEWRPLY DLCIRVMEK SKTDIGMYR CFSTLEGTL MNVIRLARI YFPASATQE ILDEFRPRL CPMSSPTIL SAIQYLEIF LPVCVKPEE AAISYDLWF EELMTLWNA CHNASAWED HMMWMIARL SSYTMGYIN WEPYIPMMF VRFSRSFQL PVAYRQKQT GKQYKIEIS AMAIWITCA LNGDKNSTF FHLEKFMQA LESYYYPAN MGRWSMKLR ELLRKLAYY FVQRVHCER YKKKTWDTN IPDHYKLTD EDIDRFVNI MKPCLEQAM FSRQGGQDI ILALQYLGS LRPNLVIPM TLDKLYASM DSLTEPHKL TSSMMGVTA VARYMVQGA RSNYPEGFT HVLPLLMAL LPGIDPNDI RKCLVTFNF MVHFINMIP LINCSEASK YHDDLTEEE HIVCEASDG FEDFVLQFF DRLCLWVES NSLDFVRLE QDANSNNNK NRAEVIAET ALFSIISTV LHQCSPEIF TAALKKVFN FVSDKVMEI QISGKMTAI ICQGFAKVN PKETLKLFI PHLCDIIEG LMNENPNIE KEEHLDAEL LYNLQILSE VVDARTELV TYSDRLMKI LDRTLHMSS LSGSQMASR MLEVMMTSL TYILPTEYR SCSTPYDTH VKDFLSIRE WGKAQNIKD LEISWYVPG DKEIALVQS LINKYLVPE VSKLSKHAS GESVLTREE LRRSLRIVI SILSSHPVL PIWDEPAIQ LVDSVLDPW AFNLIVSDS HAVNMPDGS NIRKVIADL MHKVQKKIL EVDEGDTKS IHNIVHIYN ILLFNKSRV HDFEYQWKN FHLVKKLLE DRLHQNKLH LRHILINRV MLQQEFRIE SRSCSFTET HKQILLDLF ELSVSRYSE VRISAQSKL FSVVSYFPY SYTVLTSRI FEILKLNSE EHHEKFKGC LYILISPKN TPIVARHDW NFIRQLWPL IIKSMPSEK PSIVNLINA IVDAVNRFF PTIAINLVF PQRCLDDAT LLSQNVPKC DLTHFQKYM DEGEKTLQI KCQKRAVAY NETVDGLLD ACINGNLHW RYHSMAVTF LKVLVHYDV KYNVRIVKY FLQALINES ITIRKTALR VSLFILIQN KPKFTKIEI DPYGFSNSK RNNHKLRPG IREDNKWLL YDSKTVPKD SEAWEKPRF LHDHSIGYY AWPTKVEIY APPSEQPHI DKRFPKLSE EEKEIYEFF SNAENLDQF IKYFSMEEK KGKDQFNAY KFLLFKNLF KLFEDKLLD PIIPHLEKL VADKNESSQ RCAAEIISG LIRGSKHWS FDKITKLWS VLIPILETA VVNMCSETM TDWGLSMAM ALDSRDPNR YHWILEYLV KDPLSDQTS FVACGRLHM LHAALNQQT WRNNELSVR LFDYFKQHL SHPFQNIRD KISSCLTMI FSKDLIFPD DANDTNSAG IKAFFKEIM PTLDNLYSY TLSKIESKD NPMDTSEVS KDMQGLSLE NDEVRENAM RLFKLVSKY VTSSVVRMN YSTLPEYFE ILPLSCVLQ SNDTDDEIA PICSSLLAV LAYTITIDK YMPSALEAI RKVATCPFW SARAVITEF MSGFVFHNM PTIISKKEW VLDIQHMVL ELLEDVQPE VRVSASQVL SGLLHCQFI PNPQELLAQ FKQKAKTKL KKKNLNNSN SLNLSTLRF RHAGVLGLC AFINSHPYD VPDFLPDVF GQLGPHLND PQPIPATIR KTLGDFKRT HHENWEIHK LKFTEEELL VLSDLTVPP SYYA
- >TC013292 RA
atgaaattc tcaaaggaa gaccacatt tgttttatt aaactgttt tacgatcta ctgaccatt cctgattta gaaccgacc agaatcaat aaatgcgct tcaacgtta acccaattg ctcaagaag aagtatttg atatctaga gatgaatta cagttagaa tggcgacct ttatacgac ttgtgtatc agagtaatg gagaagagc aaaaccgat ataggaatg tatcgttgc ttttcaaca ttagagggg accctaatg aacgttatc agactagct agaatctac ttccctgca tcagccact caggaaatt ttagacgag ttcaggcct cgcttatgt ccaatgagc agccccaca atcctctca gccatccaa taccttgaa attttctta cctgtttgc gtcaaaccc gaggaagct gccattagt tacgattta tggtttgaa gaacttatg acgctgtgg aacgcttgt cataacgca agtgcttgg gaagatcac atgatgtgg atgattgcg cgactatcc agctacacc atggggtat atcaattgg gagccctac attcccatg atgtttgtg cgattttcg cgcagtttt cagttacct gtagcttat agacagaaa caaaccggc aaacaatac aaaatcgaa atttccgca atggcgatt tggataaca tgcgctttg aacggtgat aaaaattcg actttcttt catttggaa aagttcatg caagcgctg gaatcctac tactatccg gcaaatatg ggccgatgg tcgatgaag ttgagggaa ttgttgcga aaacttgcc tattatttc gtgcaaagg gtgcattgt gaacgatac aagaaaaaa acgtgggat acgaacatt ccagatcat tataaattg accgacgag gatattgat agattcgtc aatattatg aagccgtgt cttgagcag gcaatgttt tcgagacaa ggggggcaa gatatcatc ctagccttg caatatctg gggtcccta agaccaaac ctggtcatt ccaatgact ttggataaa ttgtatgct tcaatggat agtctcaca gagccgcac aaactgacg tcgtccatg atgggcgtg acggcagtg gcaaggtac atggtacaa ggggcgcgc agcaactac ccagaagga ttcacccat gtgcttcca ctcctaatg gccttactc ccagggatt gacccgaac gatatccga aagtgttta gtgactttc aacttcatg gttcatttt attaacatg attccgttg atcaattgt tccgaagcg agtaaatat cacgatgac ttgacggaa gaagaacac atagtttgc gaagccagc gatggtttc gaagatttc gttttgcaa ttttttgac agactgtgt ctttgggtc gagtctaat tccctagac tttgttaga ctagaacag gatgctaac agcaacaat aataaaaat cgggccgaa gtgatcgcc gaaacggcc cttttctcg atcatttca acagtttta caccagtgc tcgccagaa attttcaca gccgctctt aaaaaagtg ttcaatttc gtaagtgac aaagtaatg gagatacaa atttcgggc aaaatgacg gcgattata tgccaaggc tttgccaaa gtcaatcct aaggaaacc ctgaaactg ttcattcca cacttgtgt gatatcatc gaaggctta atgaacgaa aacccaaac atagaaaaa gaggaacat ctcgacgct gaattgctt tacaatttg caaatttta tcagaagtt gtggatgcc aggaccgaa cttgttacg tattccgac aggttgatg aaaatcctt gataggact ttacacatg agttcgttg agtggtagt cagatggca tcacgcatg cttgaagtt atgatgacc tcactgact tacatactg ccaactgaa taccgaagt tgttcgaca ccgtatgac acacatgtt aaggacttt ctctcgata agagagtgg ggcaaagct caaaatatc aaagatctg gaaatctcg tggtacgtc cctggagat aaggaaatc gccttagtt cagtcactc attaataag tatttggta cccgaagtt tcaaaactt agcaaacac gcctctgga gagtcagtc ctgacgaga gaagagctg cgaaggtcg cttaggata gtaatttcg atattgtca tcacatcca gtgttgcca atctgggac gaacctgcg atacagctg gtcgactcg gttttagat ccgtgggct ttcaatcta atcgtgagc gattctcat gctgtaaat atgccagac ggctcaaac atccgaaaa gtgatagca gatttgatg cataaagtc cagaaaaaa atcctagaa gtggacgaa ggtgacact aaaagcatc cacaacatt gtccatatc tacaatata cttcttttc aacaaatca cgtgttcat gatttcgag tatcaatgg aagaacttc cacttggtg aaaaaacta ctcgaagac cgtttacac caaaataaa ctgcacttg cgacatatc cttatcaac agagtgatg ttgcagcaa gaatttaga atagaaagt cgtagctgt tcattcaca gaaacccac aaacagatt cttttggac ctgtttgaa ctaagtgtg tcgcgatat agcgaggtg agaatttca gcacagtcg aagctattt tctgtagtg tcatacttc ccatactct tacaccgtt ctcacatcg cgtatattc gaaatctta aaactaaac tcagaagaa caccatgag aagttcaag ggttgttta tacattttg ataagtccg aaaaataca ccaatagtg gcacgtcac gactggaat tttattcgc caattgtgg ccacttatc atcaagtcg atgccatcc gaaaaaccc tcaattgtc aatttgata aatgcgata gtcgacgct gttaaccga tttttcccg accattgcc attaacttg gtttttccg caaagatgt ctagacgat gccactttg ctaagtcaa aatgtgccc aaatgcgac ctgacccac ttccaaaaa tatatggac gagggcgag aaaacgcta caaattaaa tgccaaaag cgcgcagtt gcgtacaat gagacagtt gatgggctt ttggatgct tgtattaat ggaaatttg cactggagg tatcactca atggctgtt acgttccta aaggtgtta gtacactat gacgttaaa tacaacgtg aggatagtg aagtatttt ctacaagcc ttaataaat gagtctata accattaga aaaacggca ttaagagtc tctctgttt attttaatt cagaataaa cccaaattt acgaaaatt gaaattgat ccctacggt ttctccaac agtaaaaga aataaccac aagttgagg cccggaata agagaagac aataaatgg ttattgtac gacagtaaa acggtcccg aaagattca gaagcgtgg gaaaaaccg cgatttttg cacgatcac agcataggg tattacgct tggcctacg aaagtcgaa atatacgca cccccaagc gaacaaccc cacatagac aaaaggttc ccaaaactg agcgaagag gaaaaagag atttacgag tttttcagc aatgcggaa aatttagac caattcatt aaatatttc tcaatggag gagaagaaa gggaaagac cagttcaac gcttacaag ttcctcctg ttcaagaac ctgttcaaa cttttcgaa gacaagctc cttgacccc atcattccc cacttggag aagctcgtg gctgataaa aacgagtcg tcgcaacgt tgcgctgct gagataatc agcggttta atccgcggt tcgaagcat tggagtttt gataaaatc accaaactt tggtcagtc ctgatcccg atcctcgaa actgcagtt gtgaatatg tgtagcgaa accatgacc gactggggc ctcagtatg gcgatggct ttggactcc agagacccc aaccgctac cattggatc ttggaatac ctggtcaaa gatccactc tccgaccaa acatcgttt gtggcctgt gggcgcctc cacatgctc catgcagcc cttaaccaa caaacttgg agaaataac gaactgtca gttcgcttg ttcgactac ttcaaacag cacttgtcg caccctttc cagaatatc agggacaaa attagtagt tgtctgacg atgattttt tccaaagat ttgattttc cccgatgat gctaacgat acgaattcg gcgggaatt aaggcgttt ttcaaggag ataatgccc acacttgac aatttgtac agttacact ttgagcaaa atcgaaagt aaggataat ccaatggat acgtctgaa gtatcaaaa gatatgcaa ggactgtcg ctcgaaaac gacgaagtt cgagaaaat gccatgagg ttgttcaaa ctagtttca aaatacgtg acatcgagt gttgtaaga atgaactac tccacgctg cctgaatat ttcgagatt ttgccactg tcgtgtgtg cttcaaagc aacgacacc gacgacgaa atcgcccca atttgctcg agtttgctc gcagttttg gcctacacc ataactatt gacaaatac atgccttcg gcccttgaa gcaataaga aaagttgcc acttgtccg ttctggtcg gctcgtgcg gttataacc gaattcatg tccggtttt gtattccac aatatgccc acgattatt tcgaaaaag gagtgggta ctagatata caacacatg gtgctagaa ctactcgaa gatgttcaa cctgaagtt agagttagt gctagtcaa gttttaagc ggtcttttg cactgccaa tttattcca aaccctcaa gaattatta gcgcaattt aaacagaaa gcgaaaact aaattgaag aagaaaaat ttgaacaat tcaaacagt ttgaatctt agtacttta agatttaga catgctgga gttttgggc ttgtgtgct tttatcaat agccatcca tacgacgta cctgacttt ttaccggat gtttttgga caattagga ccccatttg aacgacccc caacccatt cctgcaaca attcgcaaa accttggga gatttcaag cgaacgcat cacgaaaac tgggaaatc cacaaactc aaatttact gaggaagaa ctgttagtt ttaagtgat ctcacagtg cctcccagt tattacgcc tag
- >TC013292 RB
atggatgaa aatgttagc aaaagacgt tacgaaaaa ctgggcttt aaaccccaa aaggagaat gtttacaac aaattactg ccctatgcc gagaaattg gacgaggaa tcgacgcaa ctgtacgcc gatattaaa actaacctt atcaagtcg gttctagca cgtgagatg agaccagga tgcgcctta tggacctca cgtttgaac aagtacatt aaaatttat ggaatgaaa ttctcaaag gaagaccac atttgtttt attaaactg ttttacgat ctactgacc attcctgat ttagaaccg accagaatc aataaatgc gcttcaacg ttaacccaa ttgctcaag aagaagtat ttgatatct agagatgaa ttacagtta gaatggcga cctttatac gacttgtgt atcagagta atggagaag agcaaaacc gatatagga atgtatcgt tgcttttca acattagag gggacccta atgaacgtt atcagacta gctagaatc tacttccct gcatcagcc actcaggaa attttagac gagttcagg cctcgctta tgtccaatg agcagcccc acaatcctc tcagccatc caatacctt gaaattttc ttacctgtt tgcgtcaaa cccgaggaa gctgccatt agttacgat ttatggttt gaagaactt atgacgctg tggaacgct tgtcataac gcaagtgct tgggaagat cacatgatg tggatgatt gcgcgacta tccagctac accatgggg tatatcaat tgggagccc tacattccc atgatgttt gtgcgattt tcgcgcagt tttcagtta cctgtagct tatagacag aaacaaacc ggcaaacaa tacaaaatc gaaatttcc gcaatggcg atttggata acatgcgct ttgaacggt gataaaaat tcgactttc tttcatttg gaaaagttc atgcaagcg ctggaatcc tactactat ccggcaaat atgggccga tggtcgatg aagttgagg gaattgttg cgaaaactt gcctattat ttcgtgcaa agggtgcat tgtgaacga tacaagaaa aaaacgtgg gatacgaac attccagat cattataaa ttgaccgac gaggatatt gatagattc gtcaatatt atgaagccg tgtcttgag caggcaatg ttttcgaga caagggggg caagatatc atcctagcc ttgcaatat ctggggtcc ctaagacca aacctggtc attccaatg actttggat aaattgtat gcttcaatg gatagtctc acagagccg cacaaactg acgtcgtcc atgatgggc gtgacggca gtggcaagg tacatggta caaggggcg cgcagcaac tacccagaa ggattcacc catgtgctt ccactccta atggcctta ctcccaggg attgacccg aacgatatc cgaaagtgt ttagtgact ttcaacttc atggttcat tttattaac atgattccg ttgatcaat tgttccgaa gcgagtaaa tatcacgat gacttgacg gaagaagaa cacatagtt tgcgaagcc agcgatggt ttcgaagat ttcgttttg caatttttt gacagactg tgtctttgg gtcgagtct aattcccta gactttgtt agactagaa caggatgct aacagcaac aataataaa aatcgggcc gaagtgatc gccgaaacg gcccttttc tcgatcatt tcaacagtt ttacaccag tgctcgcca gaaattttc acagccgct cttaaaaaa gtgttcaat ttcgtaagt gacaaagta atggagata caaatttcg ggcaaaatg acggcgatt atatgccaa ggctttgcc aaagtcaat cctaaggaa accctgaaa ctgttcatt ccacacttg tgtgatatc atcgaaggc ttaatgaac gaaaaccca aacatagaa aaagaggaa catctcgac gctgaattg ctttacaat ttgcaaatt ttatcagaa gttgtggat gccaggacc gaacttgtt acgtattcc gacaggttg atgaaaatc cttgatagg actttacac atgagttcg ttgagtggt agtcagatg gcatcacgc atgcttgaa gttatgatg acctcactg acttacata ctgccaact gaataccga agttgttcg acaccgtat gacacacat gttaaggac tttctctcg ataagagag tggggcaaa gctcaaaat atcaaagat ctggaaatc tcgtggtac gtccctgga gataaggaa atcgcctta gttcagtca ctcattaat aagtatttg gtacccgaa gtttcaaaa cttagcaaa cacgcctct ggagagtca gtcctgacg agagaagag ctgcgaagg tcgcttagg atagtaatt tcgatattg tcatcacat ccagtgttg ccaatctgg gacgaacct gcgatacag ctggtcgac tcggtttta gatccgtgg gctttcaat ctaatcgtg agcgattct catgctgta aatatgcca gacggctca aacatccga aaagtgata gcagatttg atgcataaa gtccagaaa aaaatccta gaagtggac gaaggtgac actaaaagc atccacaac attgtccat atctacaat atacttctt ttcaacaaa tcacgtgtt catgatttc gagtatcaa tggaagaac ttccacttg gtgaaaaaa ctactcgaa gaccgttta caccaaaat aaactgcac ttgcgacat atccttatc aacagagtg atgttgcag caagaattt agaatagaa agtcgtagc tgttcattc acagaaacc cacaaacag attcttttg gacctgttt gaactaagt gtgtcgcga tatagcgag gtgagaatt tcagcacag tcgaagcta ttttctgta gtgtcatac ttcccatac tcttacacc gttctcaca tcgcgtata ttcgaaatc ttaaaacta aactcagaa gaacaccat gagaagttc aagggttgt ttatacatt ttgataagt ccgaaaaat acaccaata gtggcacgt cacgactgg aattttatt cgccaattg tggccactt atcatcaag tcgatgcca tccgaaaaa ccctcaatt gtcaatttg ataaatgcg atagtcgac gctgttaac cgatttttc ccgaccatt gccattaac ttggttttt ccgcaaaga tgtctagac gatgccact ttgctaagt caaaatgtg cccaaatgc gacctgacc cacttccaa aaatatatg gacgagggc gagaaaacg ctacaaatt aaatgccaa aagcgcgca gttgcgtac aatgagaca gttgatggg cttttggat gcttgtatt aatggaaat ttgcactgg aggtatcac tcaatggct gttacgttc ctaaaggtg ttagtacac tatgacgtt aaatacaac gtgaggata gtgaagtat tttctacaa gccttaata aatgagtct ataaccatt agaaaaacg gcattaaga gtctctctg tttatttta attcagaat aaacccaaa tttacgaaa attgaaatt gatccctac ggtttctcc aacagtaaa agaaataac cacaagttg aggcccgga ataagagaa gacaataaa tggttattg tacgacagt aaaacggtc ccgaaagat tcagaagcg tgggaaaaa ccgcgattt ttgcacgat cacagcata gggtattac gcttggcct acgaaagtc gaaatatac gcaccccca agcgaacaa ccccacata gacaaaagg ttcccaaaa ctgagcgaa gaggaaaaa gagatttac gagtttttc agcaatgcg gaaaattta gaccaattc attaaatat ttctcaatg gaggagaag aaagggaaa gaccagttc aacgcttac aagttcctc ctgttcaag aacctgttc aaacttttc gaagacaag ctccttgac cccatcatt ccccacttg gagaagctc gtggctgat aaaaacgag tcgtcgcaa cgttgcgct gctgagata atcagcggt ttaatccgc ggttcgaag cattggagt tttgataaa atcaccaaa ctttggtca gtcctgatc ccgatcctc gaaactgca gttgtgaat atgtgtagc gaaaccatg accgactgg ggcctcagt atggcgatg gctttggac tccagagac cccaaccgc taccattgg atcttggaa tacctggtc aaagatcca ctctccgac caaacatcg tttgtggcc tgtgggcgc ctccacatg ctccatgca gcccttaac caacaaact tggagaaat aacgaactg tcagttcgc ttgttcgac tacttcaaa cagcacttg tcgcaccct ttccagaat atcagggac aaaattagt agttgtctg acgatgatt ttttccaaa gatttgatt ttccccgat gatgctaac gatacgaat tcggcggga attaaggcg tttttcaag gagataatg cccacactt gacaatttg tacagttac actttgagc aaaatcgaa agtaaggat aatccaatg gatacgtct gaagtatca aaagatatg caaggactg tcgctcgaa aacgacgaa gttcgagaa aatgccatg aggttgttc aaactagtt tcaaaatac gtgacatcg agtgttgta agaatgaac tactccacg ctgcctgaa tatttcgag attttgcca ctgtcgtgt gtgcttcaa agcaacgac accgacgac gaaatcgcc ccaatttgc tcgagtttg ctcgcagtt ttggcctac accataact attgacaaa tacatgcct tcggccctt gaagcaata agaaaagtt gccacttgt ccgttctgg tcggctcgt gcggttata accgaattc atgtccggt tttgtattc cacaatatg cccacgatt atttcgaaa aaggagtgg gtactagat atacaacac atggtgcta gaactactc gaagatgtt caacctgaa gttagagtt agtgctagt caagtttta agcggtctt ttgcactgc caatttatt ccaaaccct caagaatta ttagcgcaa tttaaacag aaagcgaaa actaaattg aagaagaaa aatttgaac aattcaaac agtttgaat cttagtact ttaagattt agacatgct ggagttttg ggcttgtgt gcttttatc aatagccat ccatacgac gtacctgac tttttaccg gatgttttt ggacaatta ggaccccat ttgaacgac ccccaaccc attcctgca acaattcgc aaaaccttg ggagatttc aagcgaacg catcacgaa aactgggaa atccacaaa ctcaaattt actgaggaa gaactgtta gttttaagt gatctcaca gtgcctccc agttattac gcctag
- >TC013292 RA
aggagctga atttttttt tcaggtaca ttaaaattt atggaatga aattctcaa aggaagacc acatttgtt ttattaaac tgttttacg atctactga ccattcctg atttagaac cgaccagaa tcaataaat gcgcttcaa cgttaaccc aattgctca agaagaagt atttgatat ctagagatg aattacagt tagaatggc gacctttat acgacttgt gtatcagag taatggaga agagcaaaa ccgatatag gaatgtatc gttgctttt caacattag aggggaccc taatgaacg ttatcagac tagctagaa tctacttcc ctgcatcag ccactcagg aaattttag acgagttca ggcctcgct tatgtccaa tgagcagcc ccacaatcc tctcagcca tccaatacc ttgaaattt tcttacctg tttgcgtca aacccgagg aagctgcca ttagttacg atttatggt ttgaagaac ttatgacgc tgtggaacg cttgtcata acgcaagtg cttgggaag atcacatga tgtggatga ttgcgcgac tatccagct acaccatgg ggtatatca attgggagc cctacattc ccatgatgt ttgtgcgat tttcgcgca gttttcagt tacctgtag cttatagac agaaacaaa ccggcaaac aatacaaaa tcgaaattt ccgcaatgg cgatttgga taacatgcg ctttgaacg gtgataaaa attcgactt tctttcatt tggaaaagt tcatgcaag cgctggaat cctactact atccggcaa atatgggcc gatggtcga tgaagttga gggaattgt tgcgaaaac ttgcctatt atttcgtgc aaagggtgc attgtgaac gatacaaga aaaaaacgt gggatacga acattccag atcattata aattgaccg acgaggata ttgatagat tcgtcaata ttatgaagc cgtgtcttg agcaggcaa tgttttcga gacaagggg ggcaagata tcatcctag ccttgcaat atctggggt ccctaagac caaacctgg tcattccaa tgactttgg ataaattgt atgcttcaa tggatagtc tcacagagc cgcacaaac tgacgtcgt ccatgatgg gcgtgacgg cagtggcaa ggtacatgg tacaagggg cgcgcagca actacccag aaggattca cccatgtgc ttccactcc taatggcct tactcccag ggattgacc cgaacgata tccgaaagt gtttagtga ctttcaact tcatggttc attttatta acatgattc cgttgatca attgttccg aagcgagta aatatcacg atgacttga cggaagaag aacacatag tttgcgaag ccagcgatg gtttcgaag atttcgttt tgcaatttt ttgacagac tgtgtcttt gggtcgagt ctaattccc tagactttg ttagactag aacaggatg ctaacagca acaataata aaaatcggg ccgaagtga tcgccgaaa cggcccttt tctcgatca tttcaacag ttttacacc agtgctcgc cagaaattt tcacagccg ctcttaaaa aagtgttca atttcgtaa gtgacaaag taatggaga tacaaattt cgggcaaaa tgacggcga ttatatgcc aaggctttg ccaaagtca atcctaagg aaaccctga aactgttca ttccacact tgtgtgata tcatcgaag gcttaatga acgaaaacc caaacatag aaaaagagg aacatctcg acgctgaat tgctttaca atttgcaaa ttttatcag aagttgtgg atgccagga ccgaacttg ttacgtatt ccgacaggt tgatgaaaa tccttgata ggactttac acatgagtt cgttgagtg gtagtcaga tggcatcac gcatgcttg aagttatga tgacctcac tgacttaca tactgccaa ctgaatacc gaagttgtt cgacaccgt atgacacac atgttaagg actttctct cgataagag agtggggca aagctcaaa atatcaaag atctggaaa tctcgtggt acgtccctg gagataagg aaatcgcct tagttcagt cactcatta ataagtatt tggtacccg aagtttcaa aacttagca aacacgcct ctggagagt cagtcctga cgagagaag agctgcgaa ggtcgctta ggatagtaa tttcgatat tgtcatcac atccagtgt tgccaatct gggacgaac ctgcgatac agctggtcg actcggttt tagatccgt gggctttca atctaatcg tgagcgatt ctcatgctg taaatatgc cagacggct caaacatcc gaaaagtga tagcagatt tgatgcata aagtccaga aaaaaatcc tagaagtgg acgaaggtg acactaaaa gcatccaca acattgtcc atatctaca atatacttc ttttcaaca aatcacgtg ttcatgatt tcgagtatc aatggaaga acttccact tggtgaaaa aactactcg aagaccgtt tacaccaaa ataaactgc acttgcgac atatcctta tcaacagag tgatgttgc agcaagaat ttagaatag aaagtcgta gctgttcat tcacagaaa cccacaaac agattcttt tggacctgt ttgaactaa gtgtgtcgc gatatagcg aggtgagaa tttcagcac agtcgaagc tattttctg tagtgtcat acttcccat actcttaca ccgttctca catcgcgta tattcgaaa tcttaaaac taaactcag aagaacacc atgagaagt tcaagggtt gtttataca ttttgataa gtccgaaaa atacaccaa tagtggcac gtcacgact ggaatttta ttcgccaat tgtggccac ttatcatca agtcgatgc catccgaaa aaccctcaa ttgtcaatt tgataaatg cgatagtcg acgctgtta accgatttt tcccgacca ttgccatta acttggttt ttccgcaaa gatgtctag acgatgcca ctttgctaa gtcaaaatg tgcccaaat gcgacctga cccacttcc aaaaatata tggacgagg gcgagaaaa cgctacaaa ttaaatgcc aaaagcgcg cagttgcgt acaatgaga cagttgatg ggcttttgg atgcttgta ttaatggaa atttgcact ggaggtatc actcaatgg ctgttacgt tcctaaagg tgttagtac actatgacg ttaaataca acgtgagga tagtgaagt attttctac aagccttaa taaatgagt ctataacca ttagaaaaa cggcattaa gagtctctc tgtttattt taattcaga ataaaccca aatttacga aaattgaaa ttgatccct acggtttct ccaacagta aaagaaata accacaagt tgaggcccg gaataagag aagacaata aatggttat tgtacgaca gtaaaacgg tcccgaaag attcagaag cgtgggaaa aaccgcgat ttttgcacg atcacagca tagggtatt acgcttggc ctacgaaag tcgaaatat acgcacccc caagcgaac aaccccaca tagacaaaa ggttcccaa aactgagcg aagaggaaa aagagattt acgagtttt tcagcaatg cggaaaatt tagaccaat tcattaaat atttctcaa tggaggaga agaaaggga aagaccagt tcaacgctt acaagttcc tcctgttca agaacctgt tcaaacttt tcgaagaca agctccttg accccatca ttccccact tggagaagc tcgtggctg ataaaaacg agtcgtcgc aacgttgcg ctgctgaga taatcagcg gtttaatcc gcggttcga agcattgga gttttgata aaatcacca aactttggt cagtcctga tcccgatcc tcgaaactg cagttgtga atatgtgta gcgaaacca tgaccgact ggggcctca gtatggcga tggctttgg actccagag accccaacc gctaccatt ggatcttgg aatacctgg tcaaagatc cactctccg accaaacat cgtttgtgg cctgtgggc gcctccaca tgctccatg cagccctta accaacaaa cttggagaa ataacgaac tgtcagttc gcttgttcg actacttca aacagcact tgtcgcacc ctttccaga atatcaggg acaaaatta gtagttgtc tgacgatga ttttttcca aagatttga ttttccccg atgatgcta acgatacga attcggcgg gaattaagg cgtttttca aggagataa tgcccacac ttgacaatt tgtacagtt acactttga gcaaaatcg aaagtaagg ataatccaa tggatacgt ctgaagtat caaaagata tgcaaggac tgtcgctcg aaaacgacg aagttcgag aaaatgcca tgaggttgt tcaaactag tttcaaaat acgtgacat cgagtgttg taagaatga actactcca cgctgcctg aatatttcg agattttgc cactgtcgt gtgtgcttc aaagcaacg acaccgacg acgaaatcg ccccaattt gctcgagtt tgctcgcag ttttggcct acaccataa ctattgaca aatacatgc cttcggccc ttgaagcaa taagaaaag ttgccactt gtccgttct ggtcggctc gtgcggtta taaccgaat tcatgtccg gttttgtat tccacaata tgcccacga ttatttcga aaaaggagt gggtactag atatacaac acatggtgc tagaactac tcgaagatg ttcaacctg aagttagag ttagtgcta gtcaagttt taagcggtc ttttgcact gccaattta ttccaaacc ctcaagaat tattagcgc aatttaaac agaaagcga aaactaaat tgaagaaga aaaatttga acaattcaa acagtttga atcttagta ctttaagat ttagacatg ctggagttt tgggcttgt gtgctttta tcaatagcc atccatacg acgtacctg actttttac cggatgttt ttggacaat taggacccc atttgaacg acccccaac ccattcctg caacaattc gcaaaacct tgggagatt tcaagcgaa cgcatcacg aaaactggg aaatccaca aactcaaat ttactgagg aagaactgt tagttttaa gtgatctca cagtgcctc ccagttatt acgcctagg caataattt tccgattcc agtcaattt acaatttaa ttttttact agacaatag gtgaaggaa ggtttaagg tagcaaaaa tttacgcgt caaatgttt tttattttt aaccttgtt cattatatt ttt
- >TC013292 RB
ttggcatga gatggcact ttggaaaaa tcggccgga ttggttgaa aaagtgcca ctttgtatg aaacaaata tgtctgcaa atgactagt acaaaacaa taaaaatag caagttgtt aaacaatcg tgcattgtt aatctatcg ttgtggttg ttaaagtgt gtgtgatag gttcgaggt ttgcgcgaa tcgcacgct catggatga aaatgttag caaaagacg ttacgaaaa actgggctt taaacccca aaaggagaa tgtttacaa caaattact gccctatgc cgagaaatt ggacgagga atcgacgca actgtacgc cgatattaa aactaacct tatcaagtc ggttctagc acgtgagat gagaccagg atgcgcctt atggacctc acgtttgaa caagtacat taaaattta tggaatgaa attctcaaa ggaagacca catttgttt tattaaact gttttacga tctactgac cattcctga tttagaacc gaccagaat caataaatg cgcttcaac gttaaccca attgctcaa gaagaagta tttgatatc tagagatga attacagtt agaatggcg acctttata cgacttgtg tatcagagt aatggagaa gagcaaaac cgatatagg aatgtatcg ttgcttttc aacattaga ggggaccct aatgaacgt tatcagact agctagaat ctacttccc tgcatcagc cactcagga aattttaga cgagttcag gcctcgctt atgtccaat gagcagccc cacaatcct ctcagccat ccaatacct tgaaatttt cttacctgt ttgcgtcaa acccgagga agctgccat tagttacga tttatggtt tgaagaact tatgacgct gtggaacgc ttgtcataa cgcaagtgc ttgggaaga tcacatgat gtggatgat tgcgcgact atccagcta caccatggg gtatatcaa ttgggagcc ctacattcc catgatgtt tgtgcgatt ttcgcgcag ttttcagtt acctgtagc ttatagaca gaaacaaac cggcaaaca atacaaaat cgaaatttc cgcaatggc gatttggat aacatgcgc tttgaacgg tgataaaaa ttcgacttt ctttcattt ggaaaagtt catgcaagc gctggaatc ctactacta tccggcaaa tatgggccg atggtcgat gaagttgag ggaattgtt gcgaaaact tgcctatta tttcgtgca aagggtgca ttgtgaacg atacaagaa aaaaacgtg ggatacgaa cattccaga tcattataa attgaccga cgaggatat tgatagatt cgtcaatat tatgaagcc gtgtcttga gcaggcaat gttttcgag acaaggggg gcaagatat catcctagc cttgcaata tctggggtc cctaagacc aaacctggt cattccaat gactttgga taaattgta tgcttcaat ggatagtct cacagagcc gcacaaact gacgtcgtc catgatggg cgtgacggc agtggcaag gtacatggt acaaggggc gcgcagcaa ctacccaga aggattcac ccatgtgct tccactcct aatggcctt actcccagg gattgaccc gaacgatat ccgaaagtg tttagtgac tttcaactt catggttca ttttattaa catgattcc gttgatcaa ttgttccga agcgagtaa atatcacga tgacttgac ggaagaaga acacatagt ttgcgaagc cagcgatgg tttcgaaga tttcgtttt gcaattttt tgacagact gtgtctttg ggtcgagtc taattccct agactttgt tagactaga acaggatgc taacagcaa caataataa aaatcgggc cgaagtgat cgccgaaac ggccctttt ctcgatcat ttcaacagt tttacacca gtgctcgcc agaaatttt cacagccgc tcttaaaaa agtgttcaa tttcgtaag tgacaaagt aatggagat acaaatttc gggcaaaat gacggcgat tatatgcca aggctttgc caaagtcaa tcctaagga aaccctgaa actgttcat tccacactt gtgtgatat catcgaagg cttaatgaa cgaaaaccc aaacataga aaaagagga acatctcga cgctgaatt gctttacaa tttgcaaat tttatcaga agttgtgga tgccaggac cgaacttgt tacgtattc cgacaggtt gatgaaaat ccttgatag gactttaca catgagttc gttgagtgg tagtcagat ggcatcacg catgcttga agttatgat gacctcact gacttacat actgccaac tgaataccg aagttgttc gacaccgta tgacacaca tgttaagga ctttctctc gataagaga gtggggcaa agctcaaaa tatcaaaga tctggaaat ctcgtggta cgtccctgg agataagga aatcgcctt agttcagtc actcattaa taagtattt ggtacccga agtttcaaa acttagcaa acacgcctc tggagagtc agtcctgac gagagaaga gctgcgaag gtcgcttag gatagtaat ttcgatatt gtcatcaca tccagtgtt gccaatctg ggacgaacc tgcgataca gctggtcga ctcggtttt agatccgtg ggctttcaa tctaatcgt gagcgattc tcatgctgt aaatatgcc agacggctc aaacatccg aaaagtgat agcagattt gatgcataa agtccagaa aaaaatcct agaagtgga cgaaggtga cactaaaag catccacaa cattgtcca tatctacaa tatacttct tttcaacaa atcacgtgt tcatgattt cgagtatca atggaagaa cttccactt ggtgaaaaa actactcga agaccgttt acaccaaaa taaactgca cttgcgaca tatccttat caacagagt gatgttgca gcaagaatt tagaataga aagtcgtag ctgttcatt cacagaaac ccacaaaca gattctttt ggacctgtt tgaactaag tgtgtcgcg atatagcga ggtgagaat ttcagcaca gtcgaagct attttctgt agtgtcata cttcccata ctcttacac cgttctcac atcgcgtat attcgaaat cttaaaact aaactcaga agaacacca tgagaagtt caagggttg tttatacat tttgataag tccgaaaaa tacaccaat agtggcacg tcacgactg gaattttat tcgccaatt gtggccact tatcatcaa gtcgatgcc atccgaaaa accctcaat tgtcaattt gataaatgc gatagtcga cgctgttaa ccgattttt cccgaccat tgccattaa cttggtttt tccgcaaag atgtctaga cgatgccac tttgctaag tcaaaatgt gcccaaatg cgacctgac ccacttcca aaaatatat ggacgaggg cgagaaaac gctacaaat taaatgcca aaagcgcgc agttgcgta caatgagac agttgatgg gcttttgga tgcttgtat taatggaaa tttgcactg gaggtatca ctcaatggc tgttacgtt cctaaaggt gttagtaca ctatgacgt taaatacaa cgtgaggat agtgaagta ttttctaca agccttaat aaatgagtc tataaccat tagaaaaac ggcattaag agtctctct gtttatttt aattcagaa taaacccaa atttacgaa aattgaaat tgatcccta cggtttctc caacagtaa aagaaataa ccacaagtt gaggcccgg aataagaga agacaataa atggttatt gtacgacag taaaacggt cccgaaaga ttcagaagc gtgggaaaa accgcgatt tttgcacga tcacagcat agggtatta cgcttggcc tacgaaagt cgaaatata cgcaccccc aagcgaaca accccacat agacaaaag gttcccaaa actgagcga agaggaaaa agagattta cgagttttt cagcaatgc ggaaaattt agaccaatt cattaaata tttctcaat ggaggagaa gaaagggaa agaccagtt caacgctta caagttcct cctgttcaa gaacctgtt caaactttt cgaagacaa gctccttga ccccatcat tccccactt ggagaagct cgtggctga taaaaacga gtcgtcgca acgttgcgc tgctgagat aatcagcgg tttaatccg cggttcgaa gcattggag ttttgataa aatcaccaa actttggtc agtcctgat cccgatcct cgaaactgc agttgtgaa tatgtgtag cgaaaccat gaccgactg gggcctcag tatggcgat ggctttgga ctccagaga ccccaaccg ctaccattg gatcttgga atacctggt caaagatcc actctccga ccaaacatc gtttgtggc ctgtgggcg cctccacat gctccatgc agcccttaa ccaacaaac ttggagaaa taacgaact gtcagttcg cttgttcga ctacttcaa acagcactt gtcgcaccc tttccagaa tatcaggga caaaattag tagttgtct gacgatgat tttttccaa agatttgat tttccccga tgatgctaa cgatacgaa ttcggcggg aattaaggc gtttttcaa ggagataat gcccacact tgacaattt gtacagtta cactttgag caaaatcga aagtaagga taatccaat ggatacgtc tgaagtatc aaaagatat gcaaggact gtcgctcga aaacgacga agttcgaga aaatgccat gaggttgtt caaactagt ttcaaaata cgtgacatc gagtgttgt aagaatgaa ctactccac gctgcctga atatttcga gattttgcc actgtcgtg tgtgcttca aagcaacga caccgacga cgaaatcgc cccaatttg ctcgagttt gctcgcagt tttggccta caccataac tattgacaa atacatgcc ttcggccct tgaagcaat aagaaaagt tgccacttg tccgttctg gtcggctcg tgcggttat aaccgaatt catgtccgg ttttgtatt ccacaatat gcccacgat tatttcgaa aaaggagtg ggtactaga tatacaaca catggtgct agaactact cgaagatgt tcaacctga agttagagt tagtgctag tcaagtttt aagcggtct tttgcactg ccaatttat tccaaaccc tcaagaatt attagcgca atttaaaca gaaagcgaa aactaaatt gaagaagaa aaatttgaa caattcaaa cagtttgaa tcttagtac tttaagatt tagacatgc tggagtttt gggcttgtg tgcttttat caatagcca tccatacga cgtacctga ctttttacc ggatgtttt tggacaatt aggacccca tttgaacga cccccaacc cattcctgc aacaattcg caaaacctt gggagattt caagcgaac gcatcacga aaactggga aatccacaa actcaaatt tactgagga agaactgtt agttttaag tgatctcac agtgcctcc cagttatta cgcctaggc aataatttt ccgattcca gtcaattta caatttaat tttttacta gacaatagg tgaaggaag gtttaaggt agcaaaaat ttacgcgtc aaatgtttt ttattttta accttgttc attatattt tt
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