Status
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Gene information
General information
TC013292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6384504..6394528
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013292
      iBeetle screen iB_10709

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013292 RA
        MKFSKEDHI CFIKLFYDL LTIPDLEPT RINKCASTL 
        TQLLKKKYL ISRDELQLE WRPLYDLCI RVMEKSKTD 
        IGMYRCFST LEGTLMNVI RLARIYFPA SATQEILDE 
        FRPRLCPMS SPTILSAIQ YLEIFLPVC VKPEEAAIS 
        YDLWFEELM TLWNACHNA SAWEDHMMW MIARLSSYT 
        MGYINWEPY IPMMFVRFS RSFQLPVAY RQKQTGKQY 
        KIEISAMAI WITCALNGD KNSTFFHLE KFMQALESY 
        YYPANMGRW SMKLRELLR KLAYYFVQR VHCERYKKK 
        TWDTNIPDH YKLTDEDID RFVNIMKPC LEQAMFSRQ 
        GGQDIILAL QYLGSLRPN LVIPMTLDK LYASMDSLT 
        EPHKLTSSM MGVTAVARY MVQGARSNY PEGFTHVLP 
        LLMALLPGI DPNDIRKCL VTFNFMVHF INMIPLINC 
        SEASKYHDD LTEEEHIVC EASDGFEDF VLQFFDRLC 
        LWVESNSLD FVRLEQDAN SNNNKNRAE VIAETALFS 
        IISTVLHQC SPEIFTAAL KKVFNFVSD KVMEIQISG 
        KMTAIICQG FAKVNPKET LKLFIPHLC DIIEGLMNE 
        NPNIEKEEH LDAELLYNL QILSEVVDA RTELVTYSD 
        RLMKILDRT LHMSSLSGS QMASRMLEV MMTSLTYIL 
        PTEYRSCST PYDTHVKDF LSIREWGKA QNIKDLEIS 
        WYVPGDKEI ALVQSLINK YLVPEVSKL SKHASGESV 
        LTREELRRS LRIVISILS SHPVLPIWD EPAIQLVDS 
        VLDPWAFNL IVSDSHAVN MPDGSNIRK VIADLMHKV 
        QKKILEVDE GDTKSIHNI VHIYNILLF NKSRVHDFE 
        YQWKNFHLV KKLLEDRLH QNKLHLRHI LINRVMLQQ 
        EFRIESRSC SFTETHKQI LLDLFELSV SRYSEVRIS 
        AQSKLFSVV SYFPYSYTV LTSRIFEIL KLNSEEHHE 
        KFKGCLYIL ISPKNTPIV ARHDWNFIR QLWPLIIKS 
        MPSEKPSIV NLINAIVDA VNRFFPTIA INLVFPQRC 
        LDDATLLSQ NVPKCDLTH FQKYMDEGE KTLQIKCQK 
        RAVAYNETV DGLLDACIN GNLHWRYHS MAVTFLKVL 
        VHYDVKYNV RIVKYFLQA LINESITIR KTALRVSLF 
        ILIQNKPKF TKIEIDPYG FSNSKRNNH KLRPGIRED 
        NKWLLYDSK TVPKDSEAW EKPRFLHDH SIGYYAWPT 
        KVEIYAPPS EQPHIDKRF PKLSEEEKE IYEFFSNAE 
        NLDQFIKYF SMEEKKGKD QFNAYKFLL FKNLFKLFE 
        DKLLDPIIP HLEKLVADK NESSQRCAA EIISGLIRG 
        SKHWSFDKI TKLWSVLIP ILETAVVNM CSETMTDWG 
        LSMAMALDS RDPNRYHWI LEYLVKDPL SDQTSFVAC 
        GRLHMLHAA LNQQTWRNN ELSVRLFDY FKQHLSHPF 
        QNIRDKISS CLTMIFSKD LIFPDDAND TNSAGIKAF 
        FKEIMPTLD NLYSYTLSK IESKDNPMD TSEVSKDMQ 
        GLSLENDEV RENAMRLFK LVSKYVTSS VVRMNYSTL 
        PEYFEILPL SCVLQSNDT DDEIAPICS SLLAVLAYT 
        ITIDKYMPS ALEAIRKVA TCPFWSARA VITEFMSGF 
        VFHNMPTII SKKEWVLDI QHMVLELLE DVQPEVRVS 
        ASQVLSGLL HCQFIPNPQ ELLAQFKQK AKTKLKKKN 
        LNNSNSLNL STLRFRHAG VLGLCAFIN SHPYDVPDF 
        LPDVFGQLG PHLNDPQPI PATIRKTLG DFKRTHHEN 
        WEIHKLKFT EEELLVLSD LTVPPSYYA
      • >TC013292 RB
        MDENVSKRR YEKLGFKPQ KENVYNKLL PYAEKLDEE 
        STQLYADIK TNLIKSVLA REMRPGCAL WTSRLNKYI 
        KIYGMKFSK EDHICFIKL FYDLLTIPD LEPTRINKC 
        ASTLTQLLK KKYLISRDE LQLEWRPLY DLCIRVMEK 
        SKTDIGMYR CFSTLEGTL MNVIRLARI YFPASATQE 
        ILDEFRPRL CPMSSPTIL SAIQYLEIF LPVCVKPEE 
        AAISYDLWF EELMTLWNA CHNASAWED HMMWMIARL 
        SSYTMGYIN WEPYIPMMF VRFSRSFQL PVAYRQKQT 
        GKQYKIEIS AMAIWITCA LNGDKNSTF FHLEKFMQA 
        LESYYYPAN MGRWSMKLR ELLRKLAYY FVQRVHCER 
        YKKKTWDTN IPDHYKLTD EDIDRFVNI MKPCLEQAM 
        FSRQGGQDI ILALQYLGS LRPNLVIPM TLDKLYASM 
        DSLTEPHKL TSSMMGVTA VARYMVQGA RSNYPEGFT 
        HVLPLLMAL LPGIDPNDI RKCLVTFNF MVHFINMIP 
        LINCSEASK YHDDLTEEE HIVCEASDG FEDFVLQFF 
        DRLCLWVES NSLDFVRLE QDANSNNNK NRAEVIAET 
        ALFSIISTV LHQCSPEIF TAALKKVFN FVSDKVMEI 
        QISGKMTAI ICQGFAKVN PKETLKLFI PHLCDIIEG 
        LMNENPNIE KEEHLDAEL LYNLQILSE VVDARTELV 
        TYSDRLMKI LDRTLHMSS LSGSQMASR MLEVMMTSL 
        TYILPTEYR SCSTPYDTH VKDFLSIRE WGKAQNIKD 
        LEISWYVPG DKEIALVQS LINKYLVPE VSKLSKHAS 
        GESVLTREE LRRSLRIVI SILSSHPVL PIWDEPAIQ 
        LVDSVLDPW AFNLIVSDS HAVNMPDGS NIRKVIADL 
        MHKVQKKIL EVDEGDTKS IHNIVHIYN ILLFNKSRV 
        HDFEYQWKN FHLVKKLLE DRLHQNKLH LRHILINRV 
        MLQQEFRIE SRSCSFTET HKQILLDLF ELSVSRYSE 
        VRISAQSKL FSVVSYFPY SYTVLTSRI FEILKLNSE 
        EHHEKFKGC LYILISPKN TPIVARHDW NFIRQLWPL 
        IIKSMPSEK PSIVNLINA IVDAVNRFF PTIAINLVF 
        PQRCLDDAT LLSQNVPKC DLTHFQKYM DEGEKTLQI 
        KCQKRAVAY NETVDGLLD ACINGNLHW RYHSMAVTF 
        LKVLVHYDV KYNVRIVKY FLQALINES ITIRKTALR 
        VSLFILIQN KPKFTKIEI DPYGFSNSK RNNHKLRPG 
        IREDNKWLL YDSKTVPKD SEAWEKPRF LHDHSIGYY 
        AWPTKVEIY APPSEQPHI DKRFPKLSE EEKEIYEFF 
        SNAENLDQF IKYFSMEEK KGKDQFNAY KFLLFKNLF 
        KLFEDKLLD PIIPHLEKL VADKNESSQ RCAAEIISG 
        LIRGSKHWS FDKITKLWS VLIPILETA VVNMCSETM 
        TDWGLSMAM ALDSRDPNR YHWILEYLV KDPLSDQTS 
        FVACGRLHM LHAALNQQT WRNNELSVR LFDYFKQHL 
        SHPFQNIRD KISSCLTMI FSKDLIFPD DANDTNSAG 
        IKAFFKEIM PTLDNLYSY TLSKIESKD NPMDTSEVS 
        KDMQGLSLE NDEVRENAM RLFKLVSKY VTSSVVRMN 
        YSTLPEYFE ILPLSCVLQ SNDTDDEIA PICSSLLAV 
        LAYTITIDK YMPSALEAI RKVATCPFW SARAVITEF 
        MSGFVFHNM PTIISKKEW VLDIQHMVL ELLEDVQPE 
        VRVSASQVL SGLLHCQFI PNPQELLAQ FKQKAKTKL 
        KKKNLNNSN SLNLSTLRF RHAGVLGLC AFINSHPYD 
        VPDFLPDVF GQLGPHLND PQPIPATIR KTLGDFKRT 
        HHENWEIHK LKFTEEELL VLSDLTVPP SYYA
      CDS sequence
      • >TC013292 RA
        atgaaattc tcaaaggaa gaccacatt tgttttatt 
        aaactgttt tacgatcta ctgaccatt cctgattta 
        gaaccgacc agaatcaat aaatgcgct tcaacgtta 
        acccaattg ctcaagaag aagtatttg atatctaga 
        gatgaatta cagttagaa tggcgacct ttatacgac 
        ttgtgtatc agagtaatg gagaagagc aaaaccgat 
        ataggaatg tatcgttgc ttttcaaca ttagagggg 
        accctaatg aacgttatc agactagct agaatctac 
        ttccctgca tcagccact caggaaatt ttagacgag 
        ttcaggcct cgcttatgt ccaatgagc agccccaca 
        atcctctca gccatccaa taccttgaa attttctta 
        cctgtttgc gtcaaaccc gaggaagct gccattagt 
        tacgattta tggtttgaa gaacttatg acgctgtgg 
        aacgcttgt cataacgca agtgcttgg gaagatcac 
        atgatgtgg atgattgcg cgactatcc agctacacc 
        atggggtat atcaattgg gagccctac attcccatg 
        atgtttgtg cgattttcg cgcagtttt cagttacct 
        gtagcttat agacagaaa caaaccggc aaacaatac 
        aaaatcgaa atttccgca atggcgatt tggataaca 
        tgcgctttg aacggtgat aaaaattcg actttcttt 
        catttggaa aagttcatg caagcgctg gaatcctac 
        tactatccg gcaaatatg ggccgatgg tcgatgaag 
        ttgagggaa ttgttgcga aaacttgcc tattatttc 
        gtgcaaagg gtgcattgt gaacgatac aagaaaaaa 
        acgtgggat acgaacatt ccagatcat tataaattg 
        accgacgag gatattgat agattcgtc aatattatg 
        aagccgtgt cttgagcag gcaatgttt tcgagacaa 
        ggggggcaa gatatcatc ctagccttg caatatctg 
        gggtcccta agaccaaac ctggtcatt ccaatgact 
        ttggataaa ttgtatgct tcaatggat agtctcaca 
        gagccgcac aaactgacg tcgtccatg atgggcgtg 
        acggcagtg gcaaggtac atggtacaa ggggcgcgc 
        agcaactac ccagaagga ttcacccat gtgcttcca 
        ctcctaatg gccttactc ccagggatt gacccgaac 
        gatatccga aagtgttta gtgactttc aacttcatg 
        gttcatttt attaacatg attccgttg atcaattgt 
        tccgaagcg agtaaatat cacgatgac ttgacggaa 
        gaagaacac atagtttgc gaagccagc gatggtttc 
        gaagatttc gttttgcaa ttttttgac agactgtgt 
        ctttgggtc gagtctaat tccctagac tttgttaga 
        ctagaacag gatgctaac agcaacaat aataaaaat 
        cgggccgaa gtgatcgcc gaaacggcc cttttctcg 
        atcatttca acagtttta caccagtgc tcgccagaa 
        attttcaca gccgctctt aaaaaagtg ttcaatttc 
        gtaagtgac aaagtaatg gagatacaa atttcgggc 
        aaaatgacg gcgattata tgccaaggc tttgccaaa 
        gtcaatcct aaggaaacc ctgaaactg ttcattcca 
        cacttgtgt gatatcatc gaaggctta atgaacgaa 
        aacccaaac atagaaaaa gaggaacat ctcgacgct 
        gaattgctt tacaatttg caaatttta tcagaagtt 
        gtggatgcc aggaccgaa cttgttacg tattccgac 
        aggttgatg aaaatcctt gataggact ttacacatg 
        agttcgttg agtggtagt cagatggca tcacgcatg 
        cttgaagtt atgatgacc tcactgact tacatactg 
        ccaactgaa taccgaagt tgttcgaca ccgtatgac 
        acacatgtt aaggacttt ctctcgata agagagtgg 
        ggcaaagct caaaatatc aaagatctg gaaatctcg 
        tggtacgtc cctggagat aaggaaatc gccttagtt 
        cagtcactc attaataag tatttggta cccgaagtt 
        tcaaaactt agcaaacac gcctctgga gagtcagtc 
        ctgacgaga gaagagctg cgaaggtcg cttaggata 
        gtaatttcg atattgtca tcacatcca gtgttgcca 
        atctgggac gaacctgcg atacagctg gtcgactcg 
        gttttagat ccgtgggct ttcaatcta atcgtgagc 
        gattctcat gctgtaaat atgccagac ggctcaaac 
        atccgaaaa gtgatagca gatttgatg cataaagtc 
        cagaaaaaa atcctagaa gtggacgaa ggtgacact 
        aaaagcatc cacaacatt gtccatatc tacaatata 
        cttcttttc aacaaatca cgtgttcat gatttcgag 
        tatcaatgg aagaacttc cacttggtg aaaaaacta 
        ctcgaagac cgtttacac caaaataaa ctgcacttg 
        cgacatatc cttatcaac agagtgatg ttgcagcaa 
        gaatttaga atagaaagt cgtagctgt tcattcaca 
        gaaacccac aaacagatt cttttggac ctgtttgaa 
        ctaagtgtg tcgcgatat agcgaggtg agaatttca 
        gcacagtcg aagctattt tctgtagtg tcatacttc 
        ccatactct tacaccgtt ctcacatcg cgtatattc 
        gaaatctta aaactaaac tcagaagaa caccatgag 
        aagttcaag ggttgttta tacattttg ataagtccg 
        aaaaataca ccaatagtg gcacgtcac gactggaat 
        tttattcgc caattgtgg ccacttatc atcaagtcg 
        atgccatcc gaaaaaccc tcaattgtc aatttgata 
        aatgcgata gtcgacgct gttaaccga tttttcccg 
        accattgcc attaacttg gtttttccg caaagatgt 
        ctagacgat gccactttg ctaagtcaa aatgtgccc 
        aaatgcgac ctgacccac ttccaaaaa tatatggac 
        gagggcgag aaaacgcta caaattaaa tgccaaaag 
        cgcgcagtt gcgtacaat gagacagtt gatgggctt 
        ttggatgct tgtattaat ggaaatttg cactggagg 
        tatcactca atggctgtt acgttccta aaggtgtta 
        gtacactat gacgttaaa tacaacgtg aggatagtg 
        aagtatttt ctacaagcc ttaataaat gagtctata 
        accattaga aaaacggca ttaagagtc tctctgttt 
        attttaatt cagaataaa cccaaattt acgaaaatt 
        gaaattgat ccctacggt ttctccaac agtaaaaga 
        aataaccac aagttgagg cccggaata agagaagac 
        aataaatgg ttattgtac gacagtaaa acggtcccg 
        aaagattca gaagcgtgg gaaaaaccg cgatttttg 
        cacgatcac agcataggg tattacgct tggcctacg 
        aaagtcgaa atatacgca cccccaagc gaacaaccc 
        cacatagac aaaaggttc ccaaaactg agcgaagag 
        gaaaaagag atttacgag tttttcagc aatgcggaa 
        aatttagac caattcatt aaatatttc tcaatggag 
        gagaagaaa gggaaagac cagttcaac gcttacaag 
        ttcctcctg ttcaagaac ctgttcaaa cttttcgaa 
        gacaagctc cttgacccc atcattccc cacttggag 
        aagctcgtg gctgataaa aacgagtcg tcgcaacgt 
        tgcgctgct gagataatc agcggttta atccgcggt 
        tcgaagcat tggagtttt gataaaatc accaaactt 
        tggtcagtc ctgatcccg atcctcgaa actgcagtt 
        gtgaatatg tgtagcgaa accatgacc gactggggc 
        ctcagtatg gcgatggct ttggactcc agagacccc 
        aaccgctac cattggatc ttggaatac ctggtcaaa 
        gatccactc tccgaccaa acatcgttt gtggcctgt 
        gggcgcctc cacatgctc catgcagcc cttaaccaa 
        caaacttgg agaaataac gaactgtca gttcgcttg 
        ttcgactac ttcaaacag cacttgtcg caccctttc 
        cagaatatc agggacaaa attagtagt tgtctgacg 
        atgattttt tccaaagat ttgattttc cccgatgat 
        gctaacgat acgaattcg gcgggaatt aaggcgttt 
        ttcaaggag ataatgccc acacttgac aatttgtac 
        agttacact ttgagcaaa atcgaaagt aaggataat 
        ccaatggat acgtctgaa gtatcaaaa gatatgcaa 
        ggactgtcg ctcgaaaac gacgaagtt cgagaaaat 
        gccatgagg ttgttcaaa ctagtttca aaatacgtg 
        acatcgagt gttgtaaga atgaactac tccacgctg 
        cctgaatat ttcgagatt ttgccactg tcgtgtgtg 
        cttcaaagc aacgacacc gacgacgaa atcgcccca 
        atttgctcg agtttgctc gcagttttg gcctacacc 
        ataactatt gacaaatac atgccttcg gcccttgaa 
        gcaataaga aaagttgcc acttgtccg ttctggtcg 
        gctcgtgcg gttataacc gaattcatg tccggtttt 
        gtattccac aatatgccc acgattatt tcgaaaaag 
        gagtgggta ctagatata caacacatg gtgctagaa 
        ctactcgaa gatgttcaa cctgaagtt agagttagt 
        gctagtcaa gttttaagc ggtcttttg cactgccaa 
        tttattcca aaccctcaa gaattatta gcgcaattt 
        aaacagaaa gcgaaaact aaattgaag aagaaaaat 
        ttgaacaat tcaaacagt ttgaatctt agtacttta 
        agatttaga catgctgga gttttgggc ttgtgtgct 
        tttatcaat agccatcca tacgacgta cctgacttt 
        ttaccggat gtttttgga caattagga ccccatttg 
        aacgacccc caacccatt cctgcaaca attcgcaaa 
        accttggga gatttcaag cgaacgcat cacgaaaac 
        tgggaaatc cacaaactc aaatttact gaggaagaa 
        ctgttagtt ttaagtgat ctcacagtg cctcccagt 
        tattacgcc tag
      • >TC013292 RB
        atggatgaa aatgttagc aaaagacgt tacgaaaaa 
        ctgggcttt aaaccccaa aaggagaat gtttacaac 
        aaattactg ccctatgcc gagaaattg gacgaggaa 
        tcgacgcaa ctgtacgcc gatattaaa actaacctt 
        atcaagtcg gttctagca cgtgagatg agaccagga 
        tgcgcctta tggacctca cgtttgaac aagtacatt 
        aaaatttat ggaatgaaa ttctcaaag gaagaccac 
        atttgtttt attaaactg ttttacgat ctactgacc 
        attcctgat ttagaaccg accagaatc aataaatgc 
        gcttcaacg ttaacccaa ttgctcaag aagaagtat 
        ttgatatct agagatgaa ttacagtta gaatggcga 
        cctttatac gacttgtgt atcagagta atggagaag 
        agcaaaacc gatatagga atgtatcgt tgcttttca 
        acattagag gggacccta atgaacgtt atcagacta 
        gctagaatc tacttccct gcatcagcc actcaggaa 
        attttagac gagttcagg cctcgctta tgtccaatg 
        agcagcccc acaatcctc tcagccatc caatacctt 
        gaaattttc ttacctgtt tgcgtcaaa cccgaggaa 
        gctgccatt agttacgat ttatggttt gaagaactt 
        atgacgctg tggaacgct tgtcataac gcaagtgct 
        tgggaagat cacatgatg tggatgatt gcgcgacta 
        tccagctac accatgggg tatatcaat tgggagccc 
        tacattccc atgatgttt gtgcgattt tcgcgcagt 
        tttcagtta cctgtagct tatagacag aaacaaacc 
        ggcaaacaa tacaaaatc gaaatttcc gcaatggcg 
        atttggata acatgcgct ttgaacggt gataaaaat 
        tcgactttc tttcatttg gaaaagttc atgcaagcg 
        ctggaatcc tactactat ccggcaaat atgggccga 
        tggtcgatg aagttgagg gaattgttg cgaaaactt 
        gcctattat ttcgtgcaa agggtgcat tgtgaacga 
        tacaagaaa aaaacgtgg gatacgaac attccagat 
        cattataaa ttgaccgac gaggatatt gatagattc 
        gtcaatatt atgaagccg tgtcttgag caggcaatg 
        ttttcgaga caagggggg caagatatc atcctagcc 
        ttgcaatat ctggggtcc ctaagacca aacctggtc 
        attccaatg actttggat aaattgtat gcttcaatg 
        gatagtctc acagagccg cacaaactg acgtcgtcc 
        atgatgggc gtgacggca gtggcaagg tacatggta 
        caaggggcg cgcagcaac tacccagaa ggattcacc 
        catgtgctt ccactccta atggcctta ctcccaggg 
        attgacccg aacgatatc cgaaagtgt ttagtgact 
        ttcaacttc atggttcat tttattaac atgattccg 
        ttgatcaat tgttccgaa gcgagtaaa tatcacgat 
        gacttgacg gaagaagaa cacatagtt tgcgaagcc 
        agcgatggt ttcgaagat ttcgttttg caatttttt 
        gacagactg tgtctttgg gtcgagtct aattcccta 
        gactttgtt agactagaa caggatgct aacagcaac 
        aataataaa aatcgggcc gaagtgatc gccgaaacg 
        gcccttttc tcgatcatt tcaacagtt ttacaccag 
        tgctcgcca gaaattttc acagccgct cttaaaaaa 
        gtgttcaat ttcgtaagt gacaaagta atggagata 
        caaatttcg ggcaaaatg acggcgatt atatgccaa 
        ggctttgcc aaagtcaat cctaaggaa accctgaaa 
        ctgttcatt ccacacttg tgtgatatc atcgaaggc 
        ttaatgaac gaaaaccca aacatagaa aaagaggaa 
        catctcgac gctgaattg ctttacaat ttgcaaatt 
        ttatcagaa gttgtggat gccaggacc gaacttgtt 
        acgtattcc gacaggttg atgaaaatc cttgatagg 
        actttacac atgagttcg ttgagtggt agtcagatg 
        gcatcacgc atgcttgaa gttatgatg acctcactg 
        acttacata ctgccaact gaataccga agttgttcg 
        acaccgtat gacacacat gttaaggac tttctctcg 
        ataagagag tggggcaaa gctcaaaat atcaaagat 
        ctggaaatc tcgtggtac gtccctgga gataaggaa 
        atcgcctta gttcagtca ctcattaat aagtatttg 
        gtacccgaa gtttcaaaa cttagcaaa cacgcctct 
        ggagagtca gtcctgacg agagaagag ctgcgaagg 
        tcgcttagg atagtaatt tcgatattg tcatcacat 
        ccagtgttg ccaatctgg gacgaacct gcgatacag 
        ctggtcgac tcggtttta gatccgtgg gctttcaat 
        ctaatcgtg agcgattct catgctgta aatatgcca 
        gacggctca aacatccga aaagtgata gcagatttg 
        atgcataaa gtccagaaa aaaatccta gaagtggac 
        gaaggtgac actaaaagc atccacaac attgtccat 
        atctacaat atacttctt ttcaacaaa tcacgtgtt 
        catgatttc gagtatcaa tggaagaac ttccacttg 
        gtgaaaaaa ctactcgaa gaccgttta caccaaaat 
        aaactgcac ttgcgacat atccttatc aacagagtg 
        atgttgcag caagaattt agaatagaa agtcgtagc 
        tgttcattc acagaaacc cacaaacag attcttttg 
        gacctgttt gaactaagt gtgtcgcga tatagcgag 
        gtgagaatt tcagcacag tcgaagcta ttttctgta 
        gtgtcatac ttcccatac tcttacacc gttctcaca 
        tcgcgtata ttcgaaatc ttaaaacta aactcagaa 
        gaacaccat gagaagttc aagggttgt ttatacatt 
        ttgataagt ccgaaaaat acaccaata gtggcacgt 
        cacgactgg aattttatt cgccaattg tggccactt 
        atcatcaag tcgatgcca tccgaaaaa ccctcaatt 
        gtcaatttg ataaatgcg atagtcgac gctgttaac 
        cgatttttc ccgaccatt gccattaac ttggttttt 
        ccgcaaaga tgtctagac gatgccact ttgctaagt 
        caaaatgtg cccaaatgc gacctgacc cacttccaa 
        aaatatatg gacgagggc gagaaaacg ctacaaatt 
        aaatgccaa aagcgcgca gttgcgtac aatgagaca 
        gttgatggg cttttggat gcttgtatt aatggaaat 
        ttgcactgg aggtatcac tcaatggct gttacgttc 
        ctaaaggtg ttagtacac tatgacgtt aaatacaac 
        gtgaggata gtgaagtat tttctacaa gccttaata 
        aatgagtct ataaccatt agaaaaacg gcattaaga 
        gtctctctg tttatttta attcagaat aaacccaaa 
        tttacgaaa attgaaatt gatccctac ggtttctcc 
        aacagtaaa agaaataac cacaagttg aggcccgga 
        ataagagaa gacaataaa tggttattg tacgacagt 
        aaaacggtc ccgaaagat tcagaagcg tgggaaaaa 
        ccgcgattt ttgcacgat cacagcata gggtattac 
        gcttggcct acgaaagtc gaaatatac gcaccccca 
        agcgaacaa ccccacata gacaaaagg ttcccaaaa 
        ctgagcgaa gaggaaaaa gagatttac gagtttttc 
        agcaatgcg gaaaattta gaccaattc attaaatat 
        ttctcaatg gaggagaag aaagggaaa gaccagttc 
        aacgcttac aagttcctc ctgttcaag aacctgttc 
        aaacttttc gaagacaag ctccttgac cccatcatt 
        ccccacttg gagaagctc gtggctgat aaaaacgag 
        tcgtcgcaa cgttgcgct gctgagata atcagcggt 
        ttaatccgc ggttcgaag cattggagt tttgataaa 
        atcaccaaa ctttggtca gtcctgatc ccgatcctc 
        gaaactgca gttgtgaat atgtgtagc gaaaccatg 
        accgactgg ggcctcagt atggcgatg gctttggac 
        tccagagac cccaaccgc taccattgg atcttggaa 
        tacctggtc aaagatcca ctctccgac caaacatcg 
        tttgtggcc tgtgggcgc ctccacatg ctccatgca 
        gcccttaac caacaaact tggagaaat aacgaactg 
        tcagttcgc ttgttcgac tacttcaaa cagcacttg 
        tcgcaccct ttccagaat atcagggac aaaattagt 
        agttgtctg acgatgatt ttttccaaa gatttgatt 
        ttccccgat gatgctaac gatacgaat tcggcggga 
        attaaggcg tttttcaag gagataatg cccacactt 
        gacaatttg tacagttac actttgagc aaaatcgaa 
        agtaaggat aatccaatg gatacgtct gaagtatca 
        aaagatatg caaggactg tcgctcgaa aacgacgaa 
        gttcgagaa aatgccatg aggttgttc aaactagtt 
        tcaaaatac gtgacatcg agtgttgta agaatgaac 
        tactccacg ctgcctgaa tatttcgag attttgcca 
        ctgtcgtgt gtgcttcaa agcaacgac accgacgac 
        gaaatcgcc ccaatttgc tcgagtttg ctcgcagtt 
        ttggcctac accataact attgacaaa tacatgcct 
        tcggccctt gaagcaata agaaaagtt gccacttgt 
        ccgttctgg tcggctcgt gcggttata accgaattc 
        atgtccggt tttgtattc cacaatatg cccacgatt 
        atttcgaaa aaggagtgg gtactagat atacaacac 
        atggtgcta gaactactc gaagatgtt caacctgaa 
        gttagagtt agtgctagt caagtttta agcggtctt 
        ttgcactgc caatttatt ccaaaccct caagaatta 
        ttagcgcaa tttaaacag aaagcgaaa actaaattg 
        aagaagaaa aatttgaac aattcaaac agtttgaat 
        cttagtact ttaagattt agacatgct ggagttttg 
        ggcttgtgt gcttttatc aatagccat ccatacgac 
        gtacctgac tttttaccg gatgttttt ggacaatta 
        ggaccccat ttgaacgac ccccaaccc attcctgca 
        acaattcgc aaaaccttg ggagatttc aagcgaacg 
        catcacgaa aactgggaa atccacaaa ctcaaattt 
        actgaggaa gaactgtta gttttaagt gatctcaca 
        gtgcctccc agttattac gcctag
      mRNA sequence
      • >TC013292 RA
        aggagctga atttttttt tcaggtaca ttaaaattt 
        atggaatga aattctcaa aggaagacc acatttgtt 
        ttattaaac tgttttacg atctactga ccattcctg 
        atttagaac cgaccagaa tcaataaat gcgcttcaa 
        cgttaaccc aattgctca agaagaagt atttgatat 
        ctagagatg aattacagt tagaatggc gacctttat 
        acgacttgt gtatcagag taatggaga agagcaaaa 
        ccgatatag gaatgtatc gttgctttt caacattag 
        aggggaccc taatgaacg ttatcagac tagctagaa 
        tctacttcc ctgcatcag ccactcagg aaattttag 
        acgagttca ggcctcgct tatgtccaa tgagcagcc 
        ccacaatcc tctcagcca tccaatacc ttgaaattt 
        tcttacctg tttgcgtca aacccgagg aagctgcca 
        ttagttacg atttatggt ttgaagaac ttatgacgc 
        tgtggaacg cttgtcata acgcaagtg cttgggaag 
        atcacatga tgtggatga ttgcgcgac tatccagct 
        acaccatgg ggtatatca attgggagc cctacattc 
        ccatgatgt ttgtgcgat tttcgcgca gttttcagt 
        tacctgtag cttatagac agaaacaaa ccggcaaac 
        aatacaaaa tcgaaattt ccgcaatgg cgatttgga 
        taacatgcg ctttgaacg gtgataaaa attcgactt 
        tctttcatt tggaaaagt tcatgcaag cgctggaat 
        cctactact atccggcaa atatgggcc gatggtcga 
        tgaagttga gggaattgt tgcgaaaac ttgcctatt 
        atttcgtgc aaagggtgc attgtgaac gatacaaga 
        aaaaaacgt gggatacga acattccag atcattata 
        aattgaccg acgaggata ttgatagat tcgtcaata 
        ttatgaagc cgtgtcttg agcaggcaa tgttttcga 
        gacaagggg ggcaagata tcatcctag ccttgcaat 
        atctggggt ccctaagac caaacctgg tcattccaa 
        tgactttgg ataaattgt atgcttcaa tggatagtc 
        tcacagagc cgcacaaac tgacgtcgt ccatgatgg 
        gcgtgacgg cagtggcaa ggtacatgg tacaagggg 
        cgcgcagca actacccag aaggattca cccatgtgc 
        ttccactcc taatggcct tactcccag ggattgacc 
        cgaacgata tccgaaagt gtttagtga ctttcaact 
        tcatggttc attttatta acatgattc cgttgatca 
        attgttccg aagcgagta aatatcacg atgacttga 
        cggaagaag aacacatag tttgcgaag ccagcgatg 
        gtttcgaag atttcgttt tgcaatttt ttgacagac 
        tgtgtcttt gggtcgagt ctaattccc tagactttg 
        ttagactag aacaggatg ctaacagca acaataata 
        aaaatcggg ccgaagtga tcgccgaaa cggcccttt 
        tctcgatca tttcaacag ttttacacc agtgctcgc 
        cagaaattt tcacagccg ctcttaaaa aagtgttca 
        atttcgtaa gtgacaaag taatggaga tacaaattt 
        cgggcaaaa tgacggcga ttatatgcc aaggctttg 
        ccaaagtca atcctaagg aaaccctga aactgttca 
        ttccacact tgtgtgata tcatcgaag gcttaatga 
        acgaaaacc caaacatag aaaaagagg aacatctcg 
        acgctgaat tgctttaca atttgcaaa ttttatcag 
        aagttgtgg atgccagga ccgaacttg ttacgtatt 
        ccgacaggt tgatgaaaa tccttgata ggactttac 
        acatgagtt cgttgagtg gtagtcaga tggcatcac 
        gcatgcttg aagttatga tgacctcac tgacttaca 
        tactgccaa ctgaatacc gaagttgtt cgacaccgt 
        atgacacac atgttaagg actttctct cgataagag 
        agtggggca aagctcaaa atatcaaag atctggaaa 
        tctcgtggt acgtccctg gagataagg aaatcgcct 
        tagttcagt cactcatta ataagtatt tggtacccg 
        aagtttcaa aacttagca aacacgcct ctggagagt 
        cagtcctga cgagagaag agctgcgaa ggtcgctta 
        ggatagtaa tttcgatat tgtcatcac atccagtgt 
        tgccaatct gggacgaac ctgcgatac agctggtcg 
        actcggttt tagatccgt gggctttca atctaatcg 
        tgagcgatt ctcatgctg taaatatgc cagacggct 
        caaacatcc gaaaagtga tagcagatt tgatgcata 
        aagtccaga aaaaaatcc tagaagtgg acgaaggtg 
        acactaaaa gcatccaca acattgtcc atatctaca 
        atatacttc ttttcaaca aatcacgtg ttcatgatt 
        tcgagtatc aatggaaga acttccact tggtgaaaa 
        aactactcg aagaccgtt tacaccaaa ataaactgc 
        acttgcgac atatcctta tcaacagag tgatgttgc 
        agcaagaat ttagaatag aaagtcgta gctgttcat 
        tcacagaaa cccacaaac agattcttt tggacctgt 
        ttgaactaa gtgtgtcgc gatatagcg aggtgagaa 
        tttcagcac agtcgaagc tattttctg tagtgtcat 
        acttcccat actcttaca ccgttctca catcgcgta 
        tattcgaaa tcttaaaac taaactcag aagaacacc 
        atgagaagt tcaagggtt gtttataca ttttgataa 
        gtccgaaaa atacaccaa tagtggcac gtcacgact 
        ggaatttta ttcgccaat tgtggccac ttatcatca 
        agtcgatgc catccgaaa aaccctcaa ttgtcaatt 
        tgataaatg cgatagtcg acgctgtta accgatttt 
        tcccgacca ttgccatta acttggttt ttccgcaaa 
        gatgtctag acgatgcca ctttgctaa gtcaaaatg 
        tgcccaaat gcgacctga cccacttcc aaaaatata 
        tggacgagg gcgagaaaa cgctacaaa ttaaatgcc 
        aaaagcgcg cagttgcgt acaatgaga cagttgatg 
        ggcttttgg atgcttgta ttaatggaa atttgcact 
        ggaggtatc actcaatgg ctgttacgt tcctaaagg 
        tgttagtac actatgacg ttaaataca acgtgagga 
        tagtgaagt attttctac aagccttaa taaatgagt 
        ctataacca ttagaaaaa cggcattaa gagtctctc 
        tgtttattt taattcaga ataaaccca aatttacga 
        aaattgaaa ttgatccct acggtttct ccaacagta 
        aaagaaata accacaagt tgaggcccg gaataagag 
        aagacaata aatggttat tgtacgaca gtaaaacgg 
        tcccgaaag attcagaag cgtgggaaa aaccgcgat 
        ttttgcacg atcacagca tagggtatt acgcttggc 
        ctacgaaag tcgaaatat acgcacccc caagcgaac 
        aaccccaca tagacaaaa ggttcccaa aactgagcg 
        aagaggaaa aagagattt acgagtttt tcagcaatg 
        cggaaaatt tagaccaat tcattaaat atttctcaa 
        tggaggaga agaaaggga aagaccagt tcaacgctt 
        acaagttcc tcctgttca agaacctgt tcaaacttt 
        tcgaagaca agctccttg accccatca ttccccact 
        tggagaagc tcgtggctg ataaaaacg agtcgtcgc 
        aacgttgcg ctgctgaga taatcagcg gtttaatcc 
        gcggttcga agcattgga gttttgata aaatcacca 
        aactttggt cagtcctga tcccgatcc tcgaaactg 
        cagttgtga atatgtgta gcgaaacca tgaccgact 
        ggggcctca gtatggcga tggctttgg actccagag 
        accccaacc gctaccatt ggatcttgg aatacctgg 
        tcaaagatc cactctccg accaaacat cgtttgtgg 
        cctgtgggc gcctccaca tgctccatg cagccctta 
        accaacaaa cttggagaa ataacgaac tgtcagttc 
        gcttgttcg actacttca aacagcact tgtcgcacc 
        ctttccaga atatcaggg acaaaatta gtagttgtc 
        tgacgatga ttttttcca aagatttga ttttccccg 
        atgatgcta acgatacga attcggcgg gaattaagg 
        cgtttttca aggagataa tgcccacac ttgacaatt 
        tgtacagtt acactttga gcaaaatcg aaagtaagg 
        ataatccaa tggatacgt ctgaagtat caaaagata 
        tgcaaggac tgtcgctcg aaaacgacg aagttcgag 
        aaaatgcca tgaggttgt tcaaactag tttcaaaat 
        acgtgacat cgagtgttg taagaatga actactcca 
        cgctgcctg aatatttcg agattttgc cactgtcgt 
        gtgtgcttc aaagcaacg acaccgacg acgaaatcg 
        ccccaattt gctcgagtt tgctcgcag ttttggcct 
        acaccataa ctattgaca aatacatgc cttcggccc 
        ttgaagcaa taagaaaag ttgccactt gtccgttct 
        ggtcggctc gtgcggtta taaccgaat tcatgtccg 
        gttttgtat tccacaata tgcccacga ttatttcga 
        aaaaggagt gggtactag atatacaac acatggtgc 
        tagaactac tcgaagatg ttcaacctg aagttagag 
        ttagtgcta gtcaagttt taagcggtc ttttgcact 
        gccaattta ttccaaacc ctcaagaat tattagcgc 
        aatttaaac agaaagcga aaactaaat tgaagaaga 
        aaaatttga acaattcaa acagtttga atcttagta 
        ctttaagat ttagacatg ctggagttt tgggcttgt 
        gtgctttta tcaatagcc atccatacg acgtacctg 
        actttttac cggatgttt ttggacaat taggacccc 
        atttgaacg acccccaac ccattcctg caacaattc 
        gcaaaacct tgggagatt tcaagcgaa cgcatcacg 
        aaaactggg aaatccaca aactcaaat ttactgagg 
        aagaactgt tagttttaa gtgatctca cagtgcctc 
        ccagttatt acgcctagg caataattt tccgattcc 
        agtcaattt acaatttaa ttttttact agacaatag 
        gtgaaggaa ggtttaagg tagcaaaaa tttacgcgt 
        caaatgttt tttattttt aaccttgtt cattatatt 
        ttt
      • >TC013292 RB
        ttggcatga gatggcact ttggaaaaa tcggccgga 
        ttggttgaa aaagtgcca ctttgtatg aaacaaata 
        tgtctgcaa atgactagt acaaaacaa taaaaatag 
        caagttgtt aaacaatcg tgcattgtt aatctatcg 
        ttgtggttg ttaaagtgt gtgtgatag gttcgaggt 
        ttgcgcgaa tcgcacgct catggatga aaatgttag 
        caaaagacg ttacgaaaa actgggctt taaacccca 
        aaaggagaa tgtttacaa caaattact gccctatgc 
        cgagaaatt ggacgagga atcgacgca actgtacgc 
        cgatattaa aactaacct tatcaagtc ggttctagc 
        acgtgagat gagaccagg atgcgcctt atggacctc 
        acgtttgaa caagtacat taaaattta tggaatgaa 
        attctcaaa ggaagacca catttgttt tattaaact 
        gttttacga tctactgac cattcctga tttagaacc 
        gaccagaat caataaatg cgcttcaac gttaaccca 
        attgctcaa gaagaagta tttgatatc tagagatga 
        attacagtt agaatggcg acctttata cgacttgtg 
        tatcagagt aatggagaa gagcaaaac cgatatagg 
        aatgtatcg ttgcttttc aacattaga ggggaccct 
        aatgaacgt tatcagact agctagaat ctacttccc 
        tgcatcagc cactcagga aattttaga cgagttcag 
        gcctcgctt atgtccaat gagcagccc cacaatcct 
        ctcagccat ccaatacct tgaaatttt cttacctgt 
        ttgcgtcaa acccgagga agctgccat tagttacga 
        tttatggtt tgaagaact tatgacgct gtggaacgc 
        ttgtcataa cgcaagtgc ttgggaaga tcacatgat 
        gtggatgat tgcgcgact atccagcta caccatggg 
        gtatatcaa ttgggagcc ctacattcc catgatgtt 
        tgtgcgatt ttcgcgcag ttttcagtt acctgtagc 
        ttatagaca gaaacaaac cggcaaaca atacaaaat 
        cgaaatttc cgcaatggc gatttggat aacatgcgc 
        tttgaacgg tgataaaaa ttcgacttt ctttcattt 
        ggaaaagtt catgcaagc gctggaatc ctactacta 
        tccggcaaa tatgggccg atggtcgat gaagttgag 
        ggaattgtt gcgaaaact tgcctatta tttcgtgca 
        aagggtgca ttgtgaacg atacaagaa aaaaacgtg 
        ggatacgaa cattccaga tcattataa attgaccga 
        cgaggatat tgatagatt cgtcaatat tatgaagcc 
        gtgtcttga gcaggcaat gttttcgag acaaggggg 
        gcaagatat catcctagc cttgcaata tctggggtc 
        cctaagacc aaacctggt cattccaat gactttgga 
        taaattgta tgcttcaat ggatagtct cacagagcc 
        gcacaaact gacgtcgtc catgatggg cgtgacggc 
        agtggcaag gtacatggt acaaggggc gcgcagcaa 
        ctacccaga aggattcac ccatgtgct tccactcct 
        aatggcctt actcccagg gattgaccc gaacgatat 
        ccgaaagtg tttagtgac tttcaactt catggttca 
        ttttattaa catgattcc gttgatcaa ttgttccga 
        agcgagtaa atatcacga tgacttgac ggaagaaga 
        acacatagt ttgcgaagc cagcgatgg tttcgaaga 
        tttcgtttt gcaattttt tgacagact gtgtctttg 
        ggtcgagtc taattccct agactttgt tagactaga 
        acaggatgc taacagcaa caataataa aaatcgggc 
        cgaagtgat cgccgaaac ggccctttt ctcgatcat 
        ttcaacagt tttacacca gtgctcgcc agaaatttt 
        cacagccgc tcttaaaaa agtgttcaa tttcgtaag 
        tgacaaagt aatggagat acaaatttc gggcaaaat 
        gacggcgat tatatgcca aggctttgc caaagtcaa 
        tcctaagga aaccctgaa actgttcat tccacactt 
        gtgtgatat catcgaagg cttaatgaa cgaaaaccc 
        aaacataga aaaagagga acatctcga cgctgaatt 
        gctttacaa tttgcaaat tttatcaga agttgtgga 
        tgccaggac cgaacttgt tacgtattc cgacaggtt 
        gatgaaaat ccttgatag gactttaca catgagttc 
        gttgagtgg tagtcagat ggcatcacg catgcttga 
        agttatgat gacctcact gacttacat actgccaac 
        tgaataccg aagttgttc gacaccgta tgacacaca 
        tgttaagga ctttctctc gataagaga gtggggcaa 
        agctcaaaa tatcaaaga tctggaaat ctcgtggta 
        cgtccctgg agataagga aatcgcctt agttcagtc 
        actcattaa taagtattt ggtacccga agtttcaaa 
        acttagcaa acacgcctc tggagagtc agtcctgac 
        gagagaaga gctgcgaag gtcgcttag gatagtaat 
        ttcgatatt gtcatcaca tccagtgtt gccaatctg 
        ggacgaacc tgcgataca gctggtcga ctcggtttt 
        agatccgtg ggctttcaa tctaatcgt gagcgattc 
        tcatgctgt aaatatgcc agacggctc aaacatccg 
        aaaagtgat agcagattt gatgcataa agtccagaa 
        aaaaatcct agaagtgga cgaaggtga cactaaaag 
        catccacaa cattgtcca tatctacaa tatacttct 
        tttcaacaa atcacgtgt tcatgattt cgagtatca 
        atggaagaa cttccactt ggtgaaaaa actactcga 
        agaccgttt acaccaaaa taaactgca cttgcgaca 
        tatccttat caacagagt gatgttgca gcaagaatt 
        tagaataga aagtcgtag ctgttcatt cacagaaac 
        ccacaaaca gattctttt ggacctgtt tgaactaag 
        tgtgtcgcg atatagcga ggtgagaat ttcagcaca 
        gtcgaagct attttctgt agtgtcata cttcccata 
        ctcttacac cgttctcac atcgcgtat attcgaaat 
        cttaaaact aaactcaga agaacacca tgagaagtt 
        caagggttg tttatacat tttgataag tccgaaaaa 
        tacaccaat agtggcacg tcacgactg gaattttat 
        tcgccaatt gtggccact tatcatcaa gtcgatgcc 
        atccgaaaa accctcaat tgtcaattt gataaatgc 
        gatagtcga cgctgttaa ccgattttt cccgaccat 
        tgccattaa cttggtttt tccgcaaag atgtctaga 
        cgatgccac tttgctaag tcaaaatgt gcccaaatg 
        cgacctgac ccacttcca aaaatatat ggacgaggg 
        cgagaaaac gctacaaat taaatgcca aaagcgcgc 
        agttgcgta caatgagac agttgatgg gcttttgga 
        tgcttgtat taatggaaa tttgcactg gaggtatca 
        ctcaatggc tgttacgtt cctaaaggt gttagtaca 
        ctatgacgt taaatacaa cgtgaggat agtgaagta 
        ttttctaca agccttaat aaatgagtc tataaccat 
        tagaaaaac ggcattaag agtctctct gtttatttt 
        aattcagaa taaacccaa atttacgaa aattgaaat 
        tgatcccta cggtttctc caacagtaa aagaaataa 
        ccacaagtt gaggcccgg aataagaga agacaataa 
        atggttatt gtacgacag taaaacggt cccgaaaga 
        ttcagaagc gtgggaaaa accgcgatt tttgcacga 
        tcacagcat agggtatta cgcttggcc tacgaaagt 
        cgaaatata cgcaccccc aagcgaaca accccacat 
        agacaaaag gttcccaaa actgagcga agaggaaaa 
        agagattta cgagttttt cagcaatgc ggaaaattt 
        agaccaatt cattaaata tttctcaat ggaggagaa 
        gaaagggaa agaccagtt caacgctta caagttcct 
        cctgttcaa gaacctgtt caaactttt cgaagacaa 
        gctccttga ccccatcat tccccactt ggagaagct 
        cgtggctga taaaaacga gtcgtcgca acgttgcgc 
        tgctgagat aatcagcgg tttaatccg cggttcgaa 
        gcattggag ttttgataa aatcaccaa actttggtc 
        agtcctgat cccgatcct cgaaactgc agttgtgaa 
        tatgtgtag cgaaaccat gaccgactg gggcctcag 
        tatggcgat ggctttgga ctccagaga ccccaaccg 
        ctaccattg gatcttgga atacctggt caaagatcc 
        actctccga ccaaacatc gtttgtggc ctgtgggcg 
        cctccacat gctccatgc agcccttaa ccaacaaac 
        ttggagaaa taacgaact gtcagttcg cttgttcga 
        ctacttcaa acagcactt gtcgcaccc tttccagaa 
        tatcaggga caaaattag tagttgtct gacgatgat 
        tttttccaa agatttgat tttccccga tgatgctaa 
        cgatacgaa ttcggcggg aattaaggc gtttttcaa 
        ggagataat gcccacact tgacaattt gtacagtta 
        cactttgag caaaatcga aagtaagga taatccaat 
        ggatacgtc tgaagtatc aaaagatat gcaaggact 
        gtcgctcga aaacgacga agttcgaga aaatgccat 
        gaggttgtt caaactagt ttcaaaata cgtgacatc 
        gagtgttgt aagaatgaa ctactccac gctgcctga 
        atatttcga gattttgcc actgtcgtg tgtgcttca 
        aagcaacga caccgacga cgaaatcgc cccaatttg 
        ctcgagttt gctcgcagt tttggccta caccataac 
        tattgacaa atacatgcc ttcggccct tgaagcaat 
        aagaaaagt tgccacttg tccgttctg gtcggctcg 
        tgcggttat aaccgaatt catgtccgg ttttgtatt 
        ccacaatat gcccacgat tatttcgaa aaaggagtg 
        ggtactaga tatacaaca catggtgct agaactact 
        cgaagatgt tcaacctga agttagagt tagtgctag 
        tcaagtttt aagcggtct tttgcactg ccaatttat 
        tccaaaccc tcaagaatt attagcgca atttaaaca 
        gaaagcgaa aactaaatt gaagaagaa aaatttgaa 
        caattcaaa cagtttgaa tcttagtac tttaagatt 
        tagacatgc tggagtttt gggcttgtg tgcttttat 
        caatagcca tccatacga cgtacctga ctttttacc 
        ggatgtttt tggacaatt aggacccca tttgaacga 
        cccccaacc cattcctgc aacaattcg caaaacctt 
        gggagattt caagcgaac gcatcacga aaactggga 
        aatccacaa actcaaatt tactgagga agaactgtt 
        agttttaag tgatctcac agtgcctcc cagttatta 
        cgcctaggc aataatttt ccgattcca gtcaattta 
        caatttaat tttttacta gacaatagg tgaaggaag 
        gtttaaggt agcaaaaat ttacgcgtc aaatgtttt 
        ttattttta accttgttc attatattt tt
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