Status
Please wait ...
Gene information
General information
TC005065 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7142294..7145948
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005065
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005065 RA
      MIPQVRYFY LLLFIIKCH GYQTSWNSD RRLQRIPQD 
      VMVDVVNNL ALKLLAIHN EGNDNNIAI SPYGAVSVL 
      VALKEGLRG NAAYEIQQA ANIPSDLSV IRIGLRDIH 
      RHLRSYFIP EEGFLSGLT LSHDNVTLN REYEDILKF 
      YGFDINSFN NAVFGDLFT TTVATTTTE EVTTSTTNM 
      PTTTTENMV PKEEITTQN DRLTRSEVT TTPTPSTTE 
      MVMTTTVVP TTENVVTTT KSPPTTTTV LTTQVFTSN 
      VVTTTQQVT TTPETTTTT EIKLNDETP EVTTTVKPT 
      SIETTTTNE FKLNDETPK ITTTVKPTS TEAITTTEF 
      KLNDETEEV INDNVTTEA ELTDTSATE NDSSTNEIQ 
      DETTEYLAE NETNDTLHR NTRSVVDYI IARYYDDHT 
      ITQRPPPYN SENDLYFLI NGMYKESNI NYMTYDTVL 
      PFHYIPHLN TLALRFPLD SARYYLLLL LPENPQNIN 
      KLICDLRLN GNLKYIINN LRYTHVKAI IPSFMLKGY 
      VVLTPTLQK MGIRQVFEP RQADFSQMT PDRNIYVTN 
      IEQAITVNI RNYVDPITM NNNRYLQRN NPVLFRADH 
      PFLYFVMDS EIDVTLMMG KIVNPLNSR IR
    CDS sequence
    • >TC005065 RA
      atgatcccg caagtgcgt tatttctat ttattattg 
      tttataatt aagtgtcat ggatatcaa acttcgtgg 
      aattcggat cggagactt cagagaatt ccacaagat 
      gtgatggtc gatgtggtc aacaacttg gcgttgaaa 
      ttactagcg atccacaat gaaggcaac gataacaac 
      attgcaatt tcaccatat ggtgctgta agtgtttta 
      gtagccctc aaagaaggg cttcggggg aatgcggct 
      tacgagata caacaagca gcgaatatt ccctctgac 
      ctttctgtt attcggatc ggattacga gatattcac 
      agacatctc agaagttac tttattccg gaggaaggc 
      ttcctctct ggactcact ctaagtcac gacaacgta 
      actctaaat cgcgaatat gaagatatt ttaaaattt 
      tacggcttt gatattaac agttttaac aatgcggtt 
      tttggtgat ttattcacc actactgtc gctacaacg 
      acaactgag gaagttacc acaagtaca acaaacatg 
      ccaacaacc acaactgaa aacatggtt cctaaagag 
      gaaataacg actcagaat gatagattg accagatca 
      gaagtaact acaacgcca acaccaagt acaacagag 
      atggtaatg accacaact gttgtacca acaactgag 
      aatgttgta actacgaca aaatcaccc ccgactacc 
      acaacagtt ctaacgact caagttttt acatcaaat 
      gttgtcaca acaacgcaa caagtaaca acaacccct 
      gaaacaact acgactaca gaaattaaa ttaaatgat 
      gaaacaccg gaagttaca acgacggta aaaccaacg 
      tctattgaa acaactaca actaacgaa tttaaatta 
      aacgatgaa acacccaaa attacaaca acggtaaaa 
      ccaacatct actgaagct atcacaact actgaattt 
      aaactaaac gatgaaaca gaagaagtg attaatgat 
      aatgttacc acagaagct gaactaact gatacaagt 
      gctactgaa aatgatagt tcaaccaat gaaattcag 
      gacgaaacg accgaatat ttagctgaa aacgaaact 
      aacgacact ttgcaccga aacacaagg tctgttgtg 
      gattacatc atagcgaga tattatgac gaccatact 
      attacacaa agacctccg ccgtacaat tcagaaaat 
      gatttgtac tttttaatt aacgggatg tacaaagaa 
      agcaatatt aattacatg acctacgat acggtttta 
      ccgtttcat tatatccca catttgaat actttagca 
      cttcgattt cctttagac agtgcgaga tattatctg 
      ctgttgctt cttccggaa aatccacaa aacattaac 
      aagctgatc tgtgattta agactgaat ggtaactta 
      aaatatata ataaataac ttaagatat actcatgtg 
      aaagcgatt attccgagc ttcatgttg aaaggatac 
      gttgttttg acaccgacg ttacaaaag atgggtatt 
      cggcaggtg ttcgagcca agacaggcc gatttttcc 
      cagatgacc cccgaccgg aacatttat gtaacaaac 
      atcgaacaa gccataact gtgaacatt cgaaattac 
      gtcgatcca attacgatg aataataat cgttatctg 
      caacgaaat aatccggtg ctctttcga gccgatcat 
      ccgtttctt tactttgtc atggattca gaaatcgac 
      gttacactg atgatgggc aaaattgta aatccactt 
      aactctagg attaggtaa
    mRNA sequence
    • >TC005065 RA
      aaataatat gatcccgca agtgcgtta tttctattt 
      attattgtt tataattaa gtgtcatgg atatcaaac 
      ttcgtggaa ttcggatcg gagacttca gagaattcc 
      acaagatgt gatggtcga tgtggtcaa caacttggc 
      gttgaaatt actagcgat ccacaatga aggcaacga 
      taacaacat tgcaatttc accatatgg tgctgtaag 
      tgttttagt agccctcaa agaagggct tcgggggaa 
      tgcggctta cgagataca acaagcagc gaatattcc 
      ctctgacct ttctgttat tcggatcgg attacgaga 
      tattcacag acatctcag aagttactt tattccgga 
      ggaaggctt cctctctgg actcactct aagtcacga 
      caacgtaac tctaaatcg cgaatatga agatatttt 
      aaaatttta cggctttga tattaacag ttttaacaa 
      tgcggtttt tggtgattt attcaccac tactgtcgc 
      tacaacgac aactgagga agttaccac aagtacaac 
      aaacatgcc aacaaccac aactgaaaa catggttcc 
      taaagagga aataacgac tcagaatga tagattgac 
      cagatcaga agtaactac aacgccaac accaagtac 
      aacagagat ggtaatgac cacaactgt tgtaccaac 
      aactgagaa tgttgtaac tacgacaaa atcaccccc 
      gactaccac aacagttct aacgactca agtttttac 
      atcaaatgt tgtcacaac aacgcaaca agtaacaac 
      aacccctga aacaactac gactacaga aattaaatt 
      aaatgatga aacaccgga agttacaac gacggtaaa 
      accaacgtc tattgaaac aactacaac taacgaatt 
      taaattaaa cgatgaaac acccaaaat tacaacaac 
      ggtaaaacc aacatctac tgaagctat cacaactac 
      tgaatttaa actaaacga tgaaacaga agaagtgat 
      taatgataa tgttaccac agaagctga actaactga 
      tacaagtgc tactgaaaa tgatagttc aaccaatga 
      aattcagga cgaaacgac cgaatattt agctgaaaa 
      cgaaactaa cgacacttt gcaccgaaa cacaaggtc 
      tgttgtgga ttacatcat agcgagata ttatgacga 
      ccatactat tacacaaag acctccgcc gtacaattc 
      agaaaatga tttgtactt tttaattaa cgggatgta 
      caaagaaag caatattaa ttacatgac ctacgatac 
      ggttttacc gtttcatta tatcccaca tttgaatac 
      tttagcact tcgatttcc tttagacag tgcgagata 
      ttatctgct gttgcttct tccggaaaa tccacaaaa 
      cattaacaa gctgatctg tgatttaag actgaatgg 
      taacttaaa atatataat aaataactt aagatatac 
      tcatgtgaa agcgattat tccgagctt catgttgaa 
      aggatacgt tgttttgac accgacgtt acaaaagat 
      gggtattcg gcaggtgtt cgagccaag acaggccga 
      tttttccca gatgacccc cgaccggaa catttatgt 
      aacaaacat cgaacaagc cataactgt gaacattcg 
      aaattacgt cgatccaat tacgatgaa taataatcg 
      ttatctgca acgaaataa tccggtgct ctttcgagc 
      cgatcatcc gtttcttta ctttgtcat ggattcaga 
      aatcgacgt tacactgat gatgggcaa aattgtaaa 
      tccacttaa ctctaggat taggtaaca caagtggct 
      tgtagttga gaagtttat ttaaaaata gcttaataa 
      tgaatagta gttgtaacc taatttaat aaattaata 
      ataaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY