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Gene information
General information
TC009092 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6570886..6572330
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009092
    iBeetle screen iB_10706

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009092 RA
      MFCAVFFLH FILFVQAQW EFESTNHDL CKTPHHQDG 
      KCTPIYSCK PILDLLDQV PQPLPPEIS DYLKSYHCG 
      FDGNSPKVC CPSKPIYFN NAEEPTFPD VSRHRNTYL 
      LPRKCGQFD SLDKIVNGN KTGIFEFPW MALLSYQTR 
      NGPQFKCGG TIINPRYIL TAAHCVTNL KTPLLGVRV 
      GEHDLRTVT DCETNKDKE RFCAPPFQD LQIEKVIPH 
      QQYNAKFLT DDIALLRVA PIKTSENSR PVCLPLAES 
      RNYNFTNRN AVVTGWGMT ENGRPSPDL LKVALSVVP 
      LEECKRLYK TTANLNERQ LCAGGKNKD SCGGDSGGP 
      LQVMGRIFG ETRIVQQGI VSFGPRNCG SSYLPGVYT 
      RVAAFMDWI LDNMEP
    CDS sequence
    • >TC009092 RA
      atgttttgt gccgtgttc tttctccat ttcatcctt 
      tttgtacaa gcacaatgg gaattcgaa tcgactaat 
      cacgatctc tgcaaaaca cctcaccac caagatgga 
      aaatgcacc ccgatttat tcatgcaaa ccaatcttg 
      gatttacta gaccaggta cctcaaccc ttaccacca 
      gaaatttca gattattta aaatcatac cactgcggt 
      tttgatggc aattcaccg aaagtgtgt tgcccctca 
      aaaccaatc tacttcaac aacgcagaa gaaccaaca 
      tttcccgac gtttcgaga cacagaaat acttatctg 
      ctaccgcga aaatgtggc caatttgac tcccttgac 
      aaaattgtc aacggtaac aagactggt atttttgag 
      tttccttgg atggcttta ttgtcctac caaacccga 
      aatggtccc cagtttaag tgtggcggc acaataatc 
      aaccccaga tatatttta acagcggca cattgtgtc 
      acaaacctc aaaacacca ttattgggg gttcgagtt 
      ggtgaacac gatctaaga accgttact gactgtgaa 
      accaataaa gacaaagaa agattttgc gcacctcct 
      tttcaagat ttgcaaata gagaaagta ataccacac 
      caacaatac aacgcaaaa tttttgacc gacgacatt 
      gctcttctt cgagtggct cctattaaa acttcagaa 
      aatagccgt cctgtttgc cttccgttg gccgaaagt 
      agaaattac aactttaca aaccgaaat gcggttgtg 
      acaggatgg ggaatgact gaaaatggc cgacctagt 
      cctgatttg ttaaaagtt gccctgtct gtagttcct 
      ttggaggag tgtaaaaga ctgtacaaa accactgcc 
      aatttgaat gagcgtcag ttatgtgcc gggggaaaa 
      aacaaggac tcttgtgga ggcgatagt ggagggcct 
      ctccaagtc atggggaga atatttgga gagacacgg 
      attgtacag caaggaatt gtctcattt gggcccaga 
      aattgcggc agttcttat ttgccgggg gtttacacg 
      agagtggct gctttcatg gactggatt ctcgacaat 
      atggaaccc taa
    mRNA sequence
    • >TC009092 RA
      actccaaaa gactgttgc atagttagt ttgtagata 
      gtaggtacc tatcatcaa atcatgttt tgtgccgtg 
      ttctttctc catttcatc ctttttgta caagcacaa 
      tgggaattc gaatcgact aatcacgat ctctgcaaa 
      acacctcac caccaagat ggaaaatgc accccgatt 
      tattcatgc aaaccaatc ttggattta ctagaccag 
      gtacctcaa cccttacca ccagaaatt tcagattat 
      ttaaaatca taccactgc ggttttgat ggcaattca 
      ccgaaagtg tgttgcccc tcaaaacca atctacttc 
      aacaacgca gaagaacca acatttccc gacgtttcg 
      agacacaga aatacttat ctgctaccg cgaaaatgt 
      ggccaattt gactccctt gacaaaatt gtcaacggt 
      aacaagact ggtattttt gagtttcct tggatggct 
      ttattgtcc taccaaacc cgaaatggt ccccagttt 
      aagtgtggc ggcacaata atcaacccc agatatatt 
      ttaacagcg gcacattgt gtcacaaac ctcaaaaca 
      ccattattg ggggttcga gttggtgaa cacgatcta 
      agaaccgtt actgactgt gaaaccaat aaagacaaa 
      gaaagattt tgcgcacct ccttttcaa gatttgcaa 
      atagagaaa gtaatacca caccaacaa tacaacgca 
      aaatttttg accgacgac attgctctt cttcgagtg 
      gctcctatt aaaacttca gaaaatagc cgtcctgtt 
      tgccttccg ttggccgaa agtagaaat tacaacttt 
      acaaaccga aatgcggtt gtgacagga tggggaatg 
      actgaaaat ggccgacct agtcctgat ttgttaaaa 
      gttgccctg tctgtagtt cctttggag gagtgtaaa 
      agactgtac aaaaccact gccaatttg aatgagcgt 
      cagttatgt gccggggga aaaaacaag gactcttgt 
      ggaggcgat agtggaggg cctctccaa gtcatgggg 
      agaatattt ggagagaca cggattgta cagcaagga 
      attgtctca tttgggccc agaaattgc ggcagttct 
      tatttgccg ggggtttac acgagagtg gctgctttc 
      atggactgg attctcgac aatatggaa ccctaacta 
      gactaagca cctaaatta ttgtaacgt gttttaaga 
      aaaataaac gaaatatta cgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY