Status
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Gene information
General information
TC031381
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC011131
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4212051..4217973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031381
    iBeetle screen iB_10705

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031381 RA
      MNFRPLTEE YANVATIPA NVAPVPGSS KAIERLLTK 
      RQNEHKSTL DFLRRECRK INLQIEDEI RNEGNSLKE 
      SLGKIKAQL DRETSVFKD SGEEELANL CIGTFETSV 
      QDLNNRRLA IIDQFYDKL QDLEKKRLR FFKRVFNEA 
      YRKTGKINH LLPYDLAYF FDQEITKTN EISLSNYGH 
      YAQIHTDLK LQIQEEIRV FFLNLHDLK ERHKVAVTN 
      MRWKEVIVP REHVIETTK LPFLGFGDP NYADVTLPM 
      TATDLAQWE QGIQTTLES LDANAKKLI SLYKMAVLQ 
      IFNRFFDDM KVITNTLTD ESDQASNHL NLEAISSEP 
      YYKSLLVDV AELYTCDIA ELQNLWTNI TTYMESNIN 
      NTNIFLKNS AHLWDNHFA RTDEIKKLV LKEMTNLIE 
      KHHKLAQNF EKKMYNALD KLREGSSTA KLNKHLKEV 
      NKFLDATGN LYSSQCAAE IKLLKKYYD LTEQECELL 
      ISELDRFLS ENADKEPIL ERKSWQNIN GLQEHEQVS 
      PTNSEDDLI PYQMKSCQY QVDAVNNWM FGLWEAIKS 
      YMVTCRSEL LNQAEQWME KVKTKLRKR LEIREATLR 
      PRYSRVKTM IYDVRLKEL ENHQTFMQK LENKVQKYV 
      ADSHQSVEL SKEKRVKIV ENFESEMKE IEEKLKNAT 
      KSFQVAACI EQTKTLSGE VRRKFDETF HENYSEIEQ 
      NRKNINAMI CHFGTSIKL FSEGGDFSR DEAKIYKKR 
      FQKIQKSVP KKFKKVYAE FEASKPPIL NTFRRTEIA 
      ILDRLNKSL QEFEHNEKT CDKIRQLRE NIRKLAYET 
      KFKVHRIDA CIDYFRDMS KIEIWKRKH FDIMRNALQ 
      ECIEKIVEI GNFVIGDGE NIGKSYIFQ LSQLIDSSF 
      EEIEQSAQV SLNAS
    CDS sequence
    • >TC031381 RA
      atgaatttt cggccctta actgaagaa tacgccaac 
      gtggctaca attcctgct aatgttgcc cctgtacca 
      ggctcgtcc aaagccatc gaaagactt ctcacaaaa 
      cgccaaaac gaacacaaa tccactttg gatttctta 
      cgacgtgaa tgccgcaaa attaactta caaatcgag 
      gacgaaatc aggaatgag ggaaacagt ctgaaggaa 
      agtttgggc aaaattaaa gcacaacta gatcgcgaa 
      actagtgtt tttaaagac tcgggggag gaagagctc 
      gctaatttg tgcattgga acgtttgaa acgtcggtg 
      caagatttg aataatcgc cgattggcg attattgac 
      cagttttac gataaactt caagatttg gagaaaaaa 
      agactgagg tttttcaaa cgtgttttc aatgaagcg 
      taccggaaa actggaaaa atcaaccac ttgttgccc 
      tatgacttg gcctatttt tttgaccag gaaatcaca 
      aaaactaac gaaatttca ttgagcaat tatggacac 
      tatgctcaa atacacact gatttgaaa ctacaaata 
      caggaagaa attcgtgtg ttttttcta aatttgcac 
      gatcttaaa gaaagacac aaagtggct gttaccaac 
      atgagatgg aaagaagtt atcgtcccc cgagagcac 
      gtaatcgag actacaaaa ttaccattt ttgggcttt 
      ggggacccg aattatgcc gatgttact cttccaatg 
      actgcgaca gatttggca cagtgggag caaggaatt 
      caaacgact ttggaaagt ttggacgca aatgccaaa 
      aaactgatc agtttgtat aaaatggca gtgcttcaa 
      atttttaac cgatttttc gacgatatg aaagttatc 
      acaaatact ctaactgat gagagtgat caggcgagt 
      aatcatctc aacttggaa gctatttct tctgagcct 
      tattataaa tccctgctt gtggatgtt gcagagctc 
      tacacttgt gatattgcg gaacttcaa aatttatgg 
      accaacatt acgacttac atggaatca aacattaac 
      aacactaac atatttctg aaaaactcg gcccacttg 
      tgggacaac cacttcgca cgtaccgac gaaataaaa 
      aaattggtt ttaaaagaa atgacaaac ttgatcgaa 
      aaacaccac aaattggcc caaaatttt gaaaaaaag 
      atgtacaat gctctggat aagttacga gaaggttcg 
      tccacagcc aaactcaat aaacatctc aaagaagtg 
      aataaattc ttggacgca actggaaat ttgtattcg 
      agccaatgc gcagctgag attaaattg ttgaagaaa 
      tattacgac ttgactgag caagaatgt gaattgttg 
      ataagcgaa cttgaccgg tttctgagc gaaaacgct 
      gataaggag ccgatactt gagaggaag agttggcaa 
      aatattaat ggtctgcaa gaacatgaa caagtttcg 
      ccgactaat tctgaagac gatttgatt ccttatcag 
      atgaaaagt tgccagtat caagtggac gctgtcaat 
      aattggatg tttggcctt tgggaagcg attaagtcg 
      tacatggtc acatgtcgt tccgaatta ttaaatcag 
      gctgaacaa tggatggaa aaagtcaaa actaaactt 
      cgaaaaagg ctcgaaatc agagaagcc actctcaga 
      ccaagatat tctcgagtc aaaacaatg atctatgac 
      gttaggttg aaagaactc gaaaatcat caaacattt 
      atgcaaaaa cttgaaaat aaggtacaa aaatacgtg 
      gctgactct caccaatcg gtcgaactt tccaaggaa 
      aaacgagtc aaaattgtc gaaaatttc gaatcagag 
      atgaaagaa attgaagaa aaattaaaa aatgcaaca 
      aaatcattc caagtggcg gcttgtatt gaacaaacg 
      aagacgctt tcgggcgaa gtgaggcga aaatttgac 
      gaaacgttt cacgaaaac tacagtgaa attgaacaa 
      aaccgcaaa aacatcaat gcaatgatt tgccacttt 
      ggcactagc atcaaatta ttttcagaa gggggcgat 
      tttagccgt gacgaagcc aaaatctac aagaaacgc 
      ttccagaaa atccaaaaa agcgttccg aaaaaattc 
      aaaaaggtt tatgcggag tttgaggct tccaagccc 
      ccgattttg aacactttt cgacgaact gagattgcg 
      attttggat cgattgaat aagtctttg caagagttc 
      gaacataat gaaaaaaca tgtgacaag attaggcaa 
      cttcgtgaa aatatccgc aagttggct tacgagacg 
      aaatttaaa gttcaccga atcgatgcc tgcattgat 
      tattttaga gatatgtcc aaaattgaa atttggaaa 
      cgcaaacat tttgatatt atgagaaat gcgctgcag 
      gagtgtatt gaaaaaatc gtcgaaatt gggaatttt 
      gtgattggc gatggtgag aatattggg aagagttac 
      atttttcaa ttgagtcaa ttgattgat tcctcgttt 
      gaagaaatt gaacagtcg gcacaggta agcttaaat 
      gcttcttaa
    mRNA sequence
    • >TC031381 RA
      atgtactag ttatttttt cgaaaaaaa ttttgtttt 
      gccattttg tgtcagtga cactcagtt atctgacaa 
      attgtcaaa aaattgagt taaaatgaa ttttcggcc 
      cttaactga agaatacgc caacgtggc tacaattcc 
      tgctaatgt tgcccctgt accaggctc gtccaaagc 
      catcgaaag acttctcac aaaacgcca aaacgaaca 
      caaatccac tttggattt cttacgacg tgaatgccg 
      caaaattaa cttacaaat cgaggacga aatcaggaa 
      tgagggaaa cagtctgaa ggaaagttt gggcaaaat 
      taaagcaca actagatcg cgaaactag tgtttttaa 
      agactcggg ggaggaaga gctcgctaa tttgtgcat 
      tggaacgtt tgaaacgtc ggtgcaaga tttgaataa 
      tcgccgatt ggcgattat tgaccagtt ttacgataa 
      acttcaaga tttggagaa aaaaagact gaggttttt 
      caaacgtgt tttcaatga agcgtaccg gaaaactgg 
      aaaaatcaa ccacttgtt gccctatga cttggccta 
      tttttttga ccaggaaat cacaaaaac taacgaaat 
      ttcattgag caattatgg acactatgc tcaaataca 
      cactgattt gaaactaca aatacagga agaaattcg 
      tgtgttttt tctaaattt gcacgatct taaagaaag 
      acacaaagt ggctgttac caacatgag atggaaaga 
      agttatcgt cccccgaga gcacgtaat cgagactac 
      aaaattacc atttttggg ctttgggga cccgaatta 
      tgccgatgt tactcttcc aatgactgc gacagattt 
      ggcacagtg ggagcaagg aattcaaac gactttgga 
      aagtttgga cgcaaatgc caaaaaact gatcagttt 
      gtataaaat ggcagtgct tcaaatttt taaccgatt 
      tttcgacga tatgaaagt tatcacaaa tactctaac 
      tgatgagag tgatcaggc gagtaatca tctcaactt 
      ggaagctat ttcttctga gccttatta taaatccct 
      gcttgtgga tgttgcaga gctctacac ttgtgatat 
      tgcggaact tcaaaattt atggaccaa cattacgac 
      ttacatgga atcaaacat taacaacac taacatatt 
      tctgaaaaa ctcggccca cttgtggga caaccactt 
      cgcacgtac cgacgaaat aaaaaaatt ggttttaaa 
      agaaatgac aaacttgat cgaaaaaca ccacaaatt 
      ggcccaaaa ttttgaaaa aaagatgta caatgctct 
      ggataagtt acgagaagg ttcgtccac agccaaact 
      caataaaca tctcaaaga agtgaataa attcttgga 
      cgcaactgg aaatttgta ttcgagcca atgcgcagc 
      tgagattaa attgttgaa gaaatatta cgacttgac 
      tgagcaaga atgtgaatt gttgataag cgaacttga 
      ccggtttct gagcgaaaa cgctgataa ggagccgat 
      acttgagag gaagagttg gcaaaatat taatggtct 
      gcaagaaca tgaacaagt ttcgccgac taattctga 
      agacgattt gattcctta tcagatgaa aagttgcca 
      gtatcaagt ggacgctgt caataattg gatgtttgg 
      cctttggga agcgattaa gtcgtacat ggtcacatg 
      tcgttccga attattaaa tcaggctga acaatggat 
      ggaaaaagt caaaactaa acttcgaaa aaggctcga 
      aatcagaga agccactct cagaccaag atattctcg 
      agtcaaaac aatgatcta tgacgttag gttgaaaga 
      actcgaaaa tcatcaaac atttatgca aaaacttga 
      aaataaggt acaaaaata cgtggctga ctctcacca 
      atcggtcga actttccaa ggaaaaacg agtcaaaat 
      tgtcgaaaa tttcgaatc agagatgaa agaaattga 
      agaaaaatt aaaaaatgc aacaaaatc attccaagt 
      ggcggcttg tattgaaca aacgaagac gctttcggg 
      cgaagtgag gcgaaaatt tgacgaaac gtttcacga 
      aaactacag tgaaattga acaaaaccg caaaaacat 
      caatgcaat gatttgcca ctttggcac tagcatcaa 
      attattttc agaaggggg cgattttag ccgtgacga 
      agccaaaat ctacaagaa acgcttcca gaaaatcca 
      aaaaagcgt tccgaaaaa attcaaaaa ggtttatgc 
      ggagtttga ggcttccaa gcccccgat tttgaacac 
      ttttcgacg aactgagat tgcgatttt ggatcgatt 
      gaataagtc tttgcaaga gttcgaaca taatgaaaa 
      aacatgtga caagattag gcaacttcg tgaaaatat 
      ccgcaagtt ggcttacga gacgaaatt taaagttca 
      ccgaatcga tgcctgcat tgattattt tagagatat 
      gtccaaaat tgaaatttg gaaacgcaa acattttga 
      tattatgag aaatgcgct gcaggagtg tattgaaaa 
      aatcgtcga aattgggaa ttttgtgat tggcgatgg 
      tgagaatat tgggaagag ttacatttt tcaattgag 
      tcaattgat tgattcctc gtttgaaga aattgaaca 
      gtcggcaca ggtaagctt aaatgcttc ttaaaatac 
      ttaaaatat tgccacaac aattgcaat atcacgagc 
      atatgaaca aaacctcgt taaaaacag tcccaaaaa 
      ttttctttt tttgataaa aacctcact tattttacg 
      gaaaataat gtccaaatt tgagattga aaattgaaa 
      tggtcattt tgtgggtga aaataaata attttgcga 
      aat
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