Status
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Gene information
General information
TC007632 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1997634..2000217
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007632
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10702

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007632 RA
      MPGKSHFVM FERLLIGLA EKGHNVDIA THFPTKDPP 
      ARYNQLSLV GSLPIFVNN LSLNAFDGF TNFHMIHFM 
      TKICGVDVC AATLNTPVL RDLKKTKKK YDLVVTEIF 
      GSDCMLGFG HYFKAPTVS LISSISLPW AGDRIGNPD 
      NPSYIPNYF VSTTAKMSL FERVENTIM LLYTKFLYH 
      YFSSKESNK IAKEFFGPE LPSLEELAQ NTSLVIVNS 
      HFSISHSRP TVPNFIEVG GLHIHEPKP LSKYFENLV 
      TSDANKGII YLTMGSMIM TETFDPEKL QGMFDAFSE 
      LPYKVLWKA KRENFPQGL KIPKNIHFE NWMPQMDIL 
      CHPNVKLFV SHGGLMGSQ EAVYCGVPR LGIPLFADQ 
      DNNIRASER MGLTIKVAY DDISKKTIL EASKKLLED 
      PTYKQNAER VSKQFKDRP LSALDTAIY WVEYVIRHK 
      GAPQLRSAG ADLAWYQYY LVDVVVILA SGLFVFLLL 
      TTYVLKLVL RFIYGNHKH KIE
    • >TC007632 RB
      MRSVFFIVI STFAIGQTA NILGIFPMP GKSHFVMFE 
      RLLIGLAEK GHNVDIATH FPTKDPPAR YNQLSLVGS 
      LPIFVNNLS LNAFDGFTN FHMIHFMTK ICGVDVCAA 
      TLNTPVLRD LKKTKKKYD LVVTEIFGS DCMLGFGHY 
      FKAPTVSLI SSISLPWAG DRIGNPDNP SYIPNYFVS 
      TTAKMSLFE RVENTIMLL YTKFLYHYF SSKESNKIA 
      KEFFGPELP SLEELAQNT SLVIVNSHF SISHSRPTV 
      PNFIEVGGL HIHEPKPLS KYFENLVTS DANKGIIYL 
      TMGSMIMTE TFDPEKLQG MFDAFSELP YKVLWKAKR 
      ENFPQGLKI PKNIHFENW MPQMDILCH PNVKLFVSH 
      GGLMGSQEA VYCGVPRLG IPLFADQDN NIRASERMG 
      LTIKVAYDD ISKKTILEA SKKLLEDPT YKQNAERVS 
      KQFKDRPLS ALDTAIYWV EYVIRHKGA PQLRSAGAD 
      LAWYQYYLV DVVVILASG LFVFLLLTT YVLKLVLRF 
      IYGNHKHKI E
    CDS sequence
    • >TC007632 RA
      atgcctggc aaaagccac ttcgttatg tttgaacgc 
      ctgttaata ggccttgca gaaaaaggc cacaatgtt 
      gatattgcc acccatttt ccgacaaag gatccacct 
      gccaggtat aatcagcta agtctagtc ggaagtctg 
      ccgattttc gtcaataat ttatccctc aatgcattt 
      gacggcttc acaaacttc cacatgatt cacttcatg 
      accaaaatt tgtggcgtt gatgtttgt gcagcgact 
      ctaaacacg ccagttttg cgcgactta aagaaaacc 
      aaaaaaaag tacgatctt gtggttact gaaattttc 
      ggctcggat tgtatgctg ggctttggt cactatttt 
      aaagcccca actgtctct ctaataagt agtataagt 
      ttgccatgg gccggggac aggattggg aatccagac 
      aacccctcc tacattcca aactatttt gtttcaacc 
      accgcaaaa atgagcctc tttgagaga gtcgaaaac 
      acaattatg ttgctttat accaagttt ttgtaccac 
      tacttctcg tcaaaggaa tcgaacaaa atcgcaaag 
      gaatttttc gggcccgag ttaccatct ctggaagag 
      ttggcccaa aacactagt cttgtgata gtaaatagt 
      cacttcagt atttcgcat tcaagacca actgtacct 
      aattttatt gaagtcggg gggctccac atccatgag 
      cctaaacca ttatccaaa tattttgaa aatctggtc 
      acaagtgac gccaacaaa ggcataatt tacctaaca 
      atggggtcc atgatcatg actgaaacc ttcgacccg 
      gaaaaactg caaggaatg tttgatgct ttttccgaa 
      ctcccgtac aaagtttta tggaaagcg aaacgcgaa 
      aacttccct caggggcta aaaatcccc aaaaacatt 
      catttcgaa aactggatg ccccagatg gacatttta 
      tgccacccg aacgttaag ttatttgtc tcccatggc 
      ggtttgatg gggagtcag gaagccgtc tactgtggg 
      gttccccgt ttgggaatc ccacttttc gccgatcag 
      gacaacaac atccgtgct tctgaacgc atgggactt 
      accattaaa gtggcatac gacgatatc agcaagaaa 
      actatactt gaggcttcg aaaaaatta ctagaagac 
      ccaacgtac aagcaaaat gctgaacgc gtttcgaaa 
      cagtttaaa gatcggcct ttgtctgcc ttagacacg 
      gccatttat tgggtggaa tatgtgata aggcataag 
      ggagctccg caattgcga agtgcaggg gcggacttg 
      gcctggtat cagtattat ttggtggat gttgttgtt 
      attctagct tctggactt ttcgtgttt ttgttattg 
      accacgtac gtcttgaaa ttagtgctt cgctttatt 
      tacggtaac cacaaacat aaaattgag tga
    • >TC007632 RB
      atgcgttcg gtatttttt attgttatt tcgactttc 
      gcaatcgga caaactgcc aacattctc ggaattttc 
      cccatgcct ggcaaaagc cacttcgtt atgtttgaa 
      cgcctgtta ataggcctt gcagaaaaa ggccacaat 
      gttgatatt gccacccat tttccgaca aaggatcca 
      cctgccagg tataatcag ctaagtcta gtcggaagt 
      ctgccgatt ttcgtcaat aatttatcc ctcaatgca 
      tttgacggc ttcacaaac ttccacatg attcacttc 
      atgaccaaa atttgtggc gttgatgtt tgtgcagcg 
      actctaaac acgccagtt ttgcgcgac ttaaagaaa 
      accaaaaaa aagtacgat cttgtggtt actgaaatt 
      ttcggctcg gattgtatg ctgggcttt ggtcactat 
      tttaaagcc ccaactgtc tctctaata agtagtata 
      agtttgcca tgggccggg gacaggatt gggaatcca 
      gacaacccc tcctacatt ccaaactat tttgtttca 
      accaccgca aaaatgagc ctctttgag agagtcgaa 
      aacacaatt atgttgctt tataccaag tttttgtac 
      cactacttc tcgtcaaag gaatcgaac aaaatcgca 
      aaggaattt ttcgggccc gagttacca tctctggaa 
      gagttggcc caaaacact agtcttgtg atagtaaat 
      agtcacttc agtatttcg cattcaaga ccaactgta 
      cctaatttt attgaagtc ggggggctc cacatccat 
      gagcctaaa ccattatcc aaatatttt gaaaatctg 
      gtcacaagt gacgccaac aaaggcata atttaccta 
      acaatgggg tccatgatc atgactgaa accttcgac 
      ccggaaaaa ctgcaagga atgtttgat gctttttcc 
      gaactcccg tacaaagtt ttatggaaa gcgaaacgc 
      gaaaacttc cctcagggg ctaaaaatc cccaaaaac 
      attcatttc gaaaactgg atgccccag atggacatt 
      ttatgccac ccgaacgtt aagttattt gtctcccat 
      ggcggtttg atggggagt caggaagcc gtctactgt 
      ggggttccc cgtttggga atcccactt ttcgccgat 
      caggacaac aacatccgt gcttctgaa cgcatggga 
      cttaccatt aaagtggca tacgacgat atcagcaag 
      aaaactata cttgaggct tcgaaaaaa ttactagaa 
      gacccaacg tacaagcaa aatgctgaa cgcgtttcg 
      aaacagttt aaagatcgg cctttgtct gccttagac 
      acggccatt tattgggtg gaatatgtg ataaggcat 
      aagggagct ccgcaattg cgaagtgca ggggcggac 
      ttggcctgg tatcagtat tatttggtg gatgttgtt 
      gttattcta gcttctgga cttttcgtg tttttgtta 
      ttgaccacg tacgtcttg aaattagtg cttcgcttt 
      atttacggt aaccacaaa cataaaatt gagtga
    mRNA sequence
    • >TC007632 RA
      attattact tcagtttgt ggcagagca gtatttaaa 
      aactcatag aaaagtatg tgctacatt tttttttgt 
      tatagttag ttgaagatt ttaggcgac tctctacaa 
      atcgtttgt gctgtgtca tctttaaaa taataggag 
      aaactaagt gccccaaca cgcaacaag ccaattact 
      cgccaacat cttatataa cgatcaaag cagaggatc 
      acctagtta tcacagtga gatttgcaa taaatttac 
      aatgcgttc ggtattttt tattgttat ttcgacttt 
      cgcaatcgg acaaactgc caacattct cggaatttt 
      ccccatgcc tggcaaaag ccacttcgt tatgtttga 
      acgcctgtt aataggcct tgcagaaaa aggccacaa 
      tgttgatat tgccaccca ttttccgac aaaggatcc 
      acctgccag gtataatca gctaagtct agtcggaag 
      tctgccgat tttcgtcaa taatttatc cctcaatgc 
      atttgacgg cttcacaaa cttccacat gattcactt 
      catgaccaa aatttgtgg cgttgatgt ttgtgcagc 
      gactctaaa cacgccagt tttgcgcga cttaaagaa 
      aaccaaaaa aaagtacga tcttgtggt tactgaaat 
      tttcggctc ggattgtat gctgggctt tggtcacta 
      ttttaaagc cccaactgt ctctctaat aagtagtat 
      aagtttgcc atgggccgg ggacaggat tgggaatcc 
      agacaaccc ctcctacat tccaaacta ttttgtttc 
      aaccaccgc aaaaatgag cctctttga gagagtcga 
      aaacacaat tatgttgct ttataccaa gtttttgta 
      ccactactt ctcgtcaaa ggaatcgaa caaaatcgc 
      aaaggaatt tttcgggcc cgagttacc atctctgga 
      agagttggc ccaaaacac tagtcttgt gatagtaaa 
      tagtcactt cagtatttc gcattcaag accaactgt 
      acctaattt tattgaagt cggggggct ccacatcca 
      tgagcctaa accattatc caaatattt tgaaaatct 
      ggtcacaag tgacgccaa caaaggcat aatttacct 
      aacaatggg gtccatgat catgactga aaccttcga 
      cccggaaaa actgcaagg aatgtttga tgctttttc 
      cgaactccc gtacaaagt tttatggaa agcgaaacg 
      cgaaaactt ccctcaggg gctaaaaat ccccaaaaa 
      cattcattt cgaaaactg gatgcccca gatggacat 
      tttatgcca cccgaacgt taagttatt tgtctccca 
      tggcggttt gatggggag tcaggaagc cgtctactg 
      tggggttcc ccgtttggg aatcccact tttcgccga 
      tcaggacaa caacatccg tgcttctga acgcatggg 
      acttaccat taaagtggc atacgacga tatcagcaa 
      gaaaactat acttgaggc ttcgaaaaa attactaga 
      agacccaac gtacaagca aaatgctga acgcgtttc 
      gaaacagtt taaagatcg gcctttgtc tgccttaga 
      cacggccat ttattgggt ggaatatgt gataaggca 
      taagggagc tccgcaatt gcgaagtgc aggggcgga 
      cttggcctg gtatcagta ttatttggt ggatgttgt 
      tgttattct agcttctgg acttttcgt gtttttgtt 
      attgaccac gtacgtctt gaaattagt gcttcgctt 
      tatttacgg taaccacaa acataaaat tgagtgatt 
      tattggttc ctggtattg tacataatt tgtttttgt 
      atttttttg aatattcat taactgcgt caaactata 
      aatgtaggt tttgtcgta tttttaagg ctcaacatt 
      attaaaaac acttaaact tt
    • >TC007632 RB
      attattact tcagtttgt ggcagagca gtatttaaa 
      aactcatag aaaaaagac tcatggctt ttatcaatc 
      tacaaacat gatagtcac tcgaacatc caaaactaa 
      aagcttttg taagcgcag tctttaatt tccgtgcag 
      ttggtcata atttttcat aattgaaag ttaattagt 
      gatctcacg agtactcac tacacgata attagttaa 
      ttgtgttag tatagaagc tatattttt tggtagact 
      ctctacaaa tcgtttgtg ctgtgtcat ctttaaaat 
      aataggaga aactaagtg ccccaacac gcaacaagc 
      caattactc gccaacatc ttatataac gatcaaagc 
      agaggatca cctagttat cacagtgag atttgcaat 
      aaatttaca atgcgttcg gtatttttt attgttatt 
      tcgactttc gcaatcgga caaactgcc aacattctc 
      ggaattttc cccatgcct ggcaaaagc cacttcgtt 
      atgtttgaa cgcctgtta ataggcctt gcagaaaaa 
      ggccacaat gttgatatt gccacccat tttccgaca 
      aaggatcca cctgccagg tataatcag ctaagtcta 
      gtcggaagt ctgccgatt ttcgtcaat aatttatcc 
      ctcaatgca tttgacggc ttcacaaac ttccacatg 
      attcacttc atgaccaaa atttgtggc gttgatgtt 
      tgtgcagcg actctaaac acgccagtt ttgcgcgac 
      ttaaagaaa accaaaaaa aagtacgat cttgtggtt 
      actgaaatt ttcggctcg gattgtatg ctgggcttt 
      ggtcactat tttaaagcc ccaactgtc tctctaata 
      agtagtata agtttgcca tgggccggg gacaggatt 
      gggaatcca gacaacccc tcctacatt ccaaactat 
      tttgtttca accaccgca aaaatgagc ctctttgag 
      agagtcgaa aacacaatt atgttgctt tataccaag 
      tttttgtac cactacttc tcgtcaaag gaatcgaac 
      aaaatcgca aaggaattt ttcgggccc gagttacca 
      tctctggaa gagttggcc caaaacact agtcttgtg 
      atagtaaat agtcacttc agtatttcg cattcaaga 
      ccaactgta cctaatttt attgaagtc ggggggctc 
      cacatccat gagcctaaa ccattatcc aaatatttt 
      gaaaatctg gtcacaagt gacgccaac aaaggcata 
      atttaccta acaatgggg tccatgatc atgactgaa 
      accttcgac ccggaaaaa ctgcaagga atgtttgat 
      gctttttcc gaactcccg tacaaagtt ttatggaaa 
      gcgaaacgc gaaaacttc cctcagggg ctaaaaatc 
      cccaaaaac attcatttc gaaaactgg atgccccag 
      atggacatt ttatgccac ccgaacgtt aagttattt 
      gtctcccat ggcggtttg atggggagt caggaagcc 
      gtctactgt ggggttccc cgtttggga atcccactt 
      ttcgccgat caggacaac aacatccgt gcttctgaa 
      cgcatggga cttaccatt aaagtggca tacgacgat 
      atcagcaag aaaactata cttgaggct tcgaaaaaa 
      ttactagaa gacccaacg tacaagcaa aatgctgaa 
      cgcgtttcg aaacagttt aaagatcgg cctttgtct 
      gccttagac acggccatt tattgggtg gaatatgtg 
      ataaggcat aagggagct ccgcaattg cgaagtgca 
      ggggcggac ttggcctgg tatcagtat tatttggtg 
      gatgttgtt gttattcta gcttctgga cttttcgtg 
      tttttgtta ttgaccacg tacgtcttg aaattagtg 
      cttcgcttt atttacggt aaccacaaa cataaaatt 
      gagtgattt attggttcc tggtattgt acataattt 
      gtttttgta tttttttga atattcatt aactgcgtc 
      aaactataa atgtaggtt ttgtcgtat ttttaaggc 
      tcaacatta ttaaaaaca cttaaactt t
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