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Gene information
General information
TC014985 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5416422..5419901
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014985
    iBeetle screen iB_10701

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (13 dpi)eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014985 RA
      MLVSGFSTI DCSVYNSLT GFGEPMICS 
      NSPESGLGT SHTSLPGVD SDPDQYDGH 
      AVDPESEYY EADLLAPMG ICKALYPFE 
      ATSEGSIPM AQDEELHLI EEDQGDGWT 
      RVRRMHGEF EEGFVPTSY IETTLFNSK
    CDS sequence
    • >TC014985 RA
      atgttagtc agcgggttt tcaaccatt gattgctct 
      gtctataat tcactaact ggttttgga gaaccgatg 
      atttgttca aacagtccc gaaagtgga ttgggtaca 
      tcacacaca tctctccca ggggttgac tcagatccc 
      gaccaatac gacggacac gctgtcgac ccagaaagc 
      gaatattac gaagccgat cttctcgcc ccgatgggc 
      atttgcaaa gccttgtac ccatttgaa gctacaagt 
      gaagggagc atacctatg gcacaagac gaagaactg 
      catttgatt gaagaagac cagggcgat gggtggact 
      cgtgtgcgg agaatgcat ggagagttt gaagaaggt 
      ttcgttccg acttcttat atcgaaacg actttgttc 
      aacagcaag taa
    mRNA sequence
    • >TC014985 RA
      tagaaacgt caatagaca aaacgaaat gttagtcag 
      cgggttttc aaccattga ttgctctgt ctataattc 
      actaactgg ttttggaga accgatgat ttgttcaaa 
      cagtcccga aagtggatt gggtacatc acacacatc 
      tctcccagg ggttgactc agatcccga ccaatacga 
      cggacacgc tgtcgaccc agaaagcga atattacga 
      agccgatct tctcgcccc gatgggcat ttgcaaagc 
      cttgtaccc atttgaagc tacaagtga agggagcat 
      acctatggc acaagacga agaactgca tttgattga 
      agaagacca gggcgatgg gtggactcg tgtgcggag 
      aatgcatgg agagtttga agaaggttt cgttccgac 
      ttcttatat cgaaacgac tttgttcaa cagcaagta 
      atctcgtgt gtgcgtatc tcgccagaa atatatatt 
      tttatattt tatttatgt ttttgaaac aataattct 
      tctatttat ctagttagg ttcgttagt gtaaaaatt 
      gctcagtca ttatttgtg tttgtgacc acacacgtc 
      cataaacag gcgagtctg ctaatattt ttaccattc 
      tcaaaaact taacacacg atattcata ctatatttt 
      ttctacaat taaattaaa aaattcttg ccattctaa 
      aacaaacga aaacaactt tttttaagt attgtttgc 
      aatttgttt aaaatgata ctagaaatc aatcaatat 
      tttattaat attagaaaa cttgtacgc tttttgttt 
      ttagttttc gagttgtgg taaaaagta cttgcagtc 
      tcaccttag attgctttc ggcgtaatt gagttgatt 
      aagaagttc gcttagcac aattgttac tgtatatat 
      gctcatata ctaatacta gctagctgt gagaacaat 
      ttaagcaaa ttgtaatat aaacaaata gttgtaatt 
      ttataaccg ttttttaat tgcattcat gtttcttgt 
      cacagacct gttagggcg ttaaccttt catcatagt 
      tctagtaat taaaaattc attttcaat attcgtgct 
      acaaatgta ttatttttt ctatgaaaa actcatcaa 
      gacttttaa ggacatttc tgattggtt taaaccggc 
      tgttttttc tcaacttgg tgctacatg tagccaaat 
      ggcagctgc agatttgtt aatttagaa tggttgcat 
      atcagttat ttaagcttg tccttgaaa gcttgttca 
      tgtcttgtg aaaactcgg ataaatcat aacattaat 
      gtgcatata tgactgact actgcatta taaatatca 
      acaattaaa gtgtttttt cctgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY