Status
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Gene information
General information
TC032003 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 1850204..1855794
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032003
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10695

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032003 RA
        MGDVVGFCD KIGNNLSEL GKWCVWQNI ILGQFLSLL 
        LCAINTLAH YINSGSSSV LPTGQSFPH YMFLCAIYT 
        SWLAFRRGE KGLISIIKA RGWRYLLLC LIDVQANTL 
        MSTAHQFTT LTSIQLLGC VAIPVALAL SCLVLGVRY 
        RMVHIIAVS VCLMGVGCL VWANIEDTK IDGKNQLVG 
        DMLCLCGAV LFAIVTVLQ ELSVKNTDI VEYLGLLGL 
        FGSILSGVQ MVILEKQTL ITSTWKSSS ALLSSFSAC 
        QFMFCTFSS VFLINMGTT ALHLSLLSG NFYTLIVGI 
        LLFNYKFHA LYFLSYTLS MTGVYIYAI KQTPVASRS 
        MSSNHTTQL NNTNNRSEC QLREHMISD SPERTTEIL 
        SAFGTLNSN DTFPLSHST NTTFTSFYG SHDVLNTQT 
        TSA
      CDS sequence
      • >TC032003 RA
        atgggtgac gttgtcggt ttttgcgac aaaatcggt 
        aacaatcta tcagaatta ggaaaatgg tgtgtttgg 
        caaaatata attctgggc cagttctta tcactgctt 
        ctgtgtgcg ataaacaca ctggcccac tacataaat 
        tcagggtct tcctctgtg ctaccaaca ggccaatct 
        ttccctcac tatatgttt ctgtgcgca atttacacc 
        agttggctt gcgtttcgt agaggagag aaaggtctc 
        atttctata atcaaggct agaggttgg cggtatctt 
        ttgctttgt ttgatagat gtgcaagca aacacgtta 
        atgagtact gctcaccag ttcactact ctgacaagt 
        atacagctg ctcggttgt gtcgccatc cctgtagcg 
        ttggcctta agctgtctg gtgcttggt gtgcgttac 
        cgcatggtg catattatt gctgtttct gtgtgtctt 
        atgggtgtg gggtgcctt gtgtgggct aacatcgaa 
        gacactaag attgacggt aaaaatcag ctagtggga 
        gacatgctt tgtctgtgt ggagctgtg ctattcgca 
        atagtaaca gtgctccaa gaattgtct gtgaaaaat 
        acagatatt gttgaatat ttgggtctg ttgggattg 
        ttcggaagt atacttagt ggcgttcaa atggtgatt 
        ttagaaaaa caaacactg ataacatca acatggaaa 
        agttcgagt gcattattg tcttcgttc tccgcatgt 
        cagttcatg ttttgtact ttttcatcg gtgttttta 
        ataaatatg ggaacaaca gccttacat ttatcccta 
        ctatcggga aatttttat acactaatc gtaggaatt 
        ttacttttc aattataag tttcatgcc ttatatttt 
        ctatcgtat actttatct atgactggt gtctacatc 
        tatgcaatt aagcaaaca ccagtcgct tcacgaagt 
        atgtcgtca aatcacaca acacaactc aataataca 
        aataatcga tctgagtgt cagttgagg gaacacatg 
        atttctgac agtccagaa cgtacaact gaaattctt 
        tcagctttt ggcacatta aatagcaac gatactttt 
        cccctaagt cacagtaca aacacgact tttacgtct 
        ttttatggc agtcacgat gttctcaat acccaaacg 
        accagcgca taa
      mRNA sequence
      • >TC032003 RA
        taaaaaatc aaatatggg gttgtttgg ggtgccccg 
        cccttttcc catcaaaac aaataactc ccaatgaat 
        ggatggcaa aatgggtga cgttgtcgg tttttgcga 
        caaaatcgg taacaatct atcagaatt aggaaaatg 
        gtgtgtttg gcaaaatat aattctggg ccagttctt 
        atcactgct tctgtgtgc gataaacac actggccca 
        ctacataaa ttcagggtc ttcctctgt gctaccaac 
        aggccaatc tttccctca ctatatgtt tctgtgcgc 
        aatttacac cagttggct tgcgtttcg tagaggaga 
        gaaaggtct catttctat aatcaaggc tagaggttg 
        gcggtatct tttgctttg tttgataga tgtgcaagc 
        aaacacgtt aatgagtac tgctcacca gttcactac 
        tctgacaag tatacagct gctcggttg tgtcgccat 
        ccctgtagc gttggcctt aagctgtct ggtgcttgg 
        tgtgcgtta ccgcatggt gcatattat tgctgtttc 
        tgtgtgtct tatgggtgt ggggtgcct tgtgtgggc 
        taacatcga agacactaa gattgacgg taaaaatca 
        gctagtggg agacatgct ttgtctgtg tggagctgt 
        gctattcgc aatagtaac agtgctcca agaattgtc 
        tgtgaaaaa tacagatat tgttgaata tttgggtct 
        gttgggatt gttcggaag tatacttag tggcgttca 
        aatggtgat tttagaaaa acaaacact gataacatc 
        aacatggaa aagttcgag tgcattatt gtcttcgtt 
        ctccgcatg tcagttcat gttttgtac tttttcatc 
        ggtgttttt aataaatat gggaacaac agccttaca 
        tttatccct actatcggg aaattttta tacactaat 
        cgtaggaat tttactttt caattataa gtttcatgc 
        cttatattt tctatcgta tactttatc tatgactgg 
        tgtctacat ctatgcaat taagcaaac accagtcgc 
        ttcacgaag tatgtcgtc aaatcacac aacacaact 
        caataatac aaataatcg atctgagtg tcagttgag 
        ggaacacat gatttctga cagtccaga acgtacaac 
        tgaaattct ttcagcttt tggcacatt aaatagcaa 
        cgatacttt tcccctaag tcacagtac aaacacgac 
        ttttacgtc tttttatgg cagtcacga tgttctcaa 
        tacccaaac gaccagcgc ataaaacac aactggcct 
        taactgcac tcccatgaa atgtgtggc catcaacta 
        gtttttttg tacaatatg taagctaaa ctgtaggat 
        ccgatccaa aattaacga aaaatttat taaatattt 
        tttaaaata acagaaata acttgaact ttttgtaag 
        gatttactt gattaaaat gatagatga acatcaact 
        cttaagaga tttgaaaaa ttgaaactg atcggaccc 
        taaaattta gccccattt ttttagcga cacgacata 
        atcaagaac aaaaattta cctactgta ctgtattaa 
        gagtgttgt gtatgaaaa tctttttgt gtagcattt 
        tacgcaacg ttggaatta aaatatttc gaagtgttc 
        tcactactg gtcgttatg cgaatttaa acgacaaac 
        ttttgtata tttatatac ccattttca atgtgtatt 
        agtcgattg taaccggtt ggtgtagat acagcgaga 
        cttttaata aaagttttt atttact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY