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Gene information
General information
TC034023
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005862 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6263725..6264472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC034023
      iBeetle screen iB_10694

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Fertility
      mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

      head not present - Penetrance (percentage of animals/eggs on slide): more than 80%

      legs randomsize decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs segment fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax segment constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
      thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
      abdomen segment posterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment posterior dorsalbent - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen bristle potentially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment posterior everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      abdomen bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034023 RA
        MKVLQFLNV PLVLATLDL NFAELKYLS 
DHLLPEECR RLVAAAHFK SYMLPNELW 
QAEQALPKD ESCIDLLLH WNSAVGEGR 
GDTHETLEH RLRQMGRYD LADWLGRTV 
FHQLGEDLE RSLTEGLGD LANETTAAD 
NFSMPTLVP FVEYSDPSG DWGPFDTIC 
LLLVGALII AIFGICCYT LYLNICGKF 
KNR
      CDS sequence
      • >TC034023 RA
        atgaaagtc cttcaattt ttgaatgtg cccctcgtc 
ttggccact ctagactta aattttgcc gaactgaaa 
tacctctcc gaccacctc ctgcctgaa gaatgccgc 
cgtttggtg gcggccgcc catttcaaa tcgtacatg 
ctgccaaac gagttatgg caagccgaa caagcattg 
ccgaaagat gagtcttgt attgacctc ctcctacac 
tggaactct gctgtgggt gaaggtaga ggggacacc 
catgaaact ttggaacac agacttagg caaatgggg 
cgctatgac cttgctgac tggttgggg agaactgtt 
tttcaccaa ttgggtgaa gatttagag aggagtttg 
acggaaggt ttgggggat ttggcaaat gaaacaact 
gcagcagat aatttttca atgccgact ctggtccca 
tttgtggaa tatagtgat ccaagtggc gactgggga 
ccgtttgat acgatctgt ttgctgttg gttggtgct 
ttgataatc gcaattttt ggtatttgt tgttacacc 
ctgtatttg aatatttgc ggcaaattt aaaaacagg 
taa
      mRNA sequence
      • >TC034023 RA
        tgacgcagt cagaatgaa agtccttca atttttgaa 
tgtgcccct cgtcttggc cactctaga cttaaattt 
tgccgaact gaaatacct ctccgacca cctcctgcc 
tgaagaatg ccgccgttt ggtggcggc cgcccattt 
caaatcgta catgctgcc aaacgagtt atggcaagc 
cgaacaagc attgccgaa agatgagtc ttgtattga 
cctcctcct acactggaa ctctgctgt gggtgaagg 
tagagggga cacccatga aactttgga acacagact 
taggcaaat ggggcgcta tgaccttgc tgactggtt 
ggggagaac tgtttttca ccaattggg tgaagattt 
agagaggag tttgacgga aggtttggg ggatttggc 
aaatgaaac aactgcagc agataattt ttcaatgcc 
gactctggt cccatttgt ggaatatag tgatccaag 
tggcgactg gggaccgtt tgatacgat ctgtttgct 
gttggttgg tgctttgat aatcgcaat ttttggtat 
ttgttgtta caccctgta tttgaatat ttgcggcaa 
atttaaaaa caggtaatt ttaaaatac tggatgctg 
acaaggaat ttgtacttc cgggcgagt gtagattgt 
caaaaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY