Status
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Gene information
General information
TC005182 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4648304..4655131
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005182
    iBeetle screen iB_10688

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment:
    larva fragmented - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005182 RA
      MTVDFNDYF WGEKNNGFD VLYHNMKYG LVASKDLAE 
      LLRERSNIE ENNSKLLAK FAKQAGSCC NQGTFAPLW 
      QVLKTSAER LSALHMQMV QKVSDLVKE VNKYAEELH 
      KKHKTVKED ESGTLESVQ AMQNVTLMV QKCKDTYTQ 
      RGQELDKLR KDNASPKEI EKAELKMKK AQEEYKSYV 
      EKYTVIKDE FEKKMSVTC KNFQELEVA HLGHMKEFL 
      NSYAEVVEW THEQMGKVH KEFRQQCLE LTVDQLLEQ 
      FVRNKSTGL ERPGIVDIE EALMSPTQD SESSKPSKR 
      EGFLRSRRE KAKQKKSKK KVKDNVDTQ SNKEEKSDM 
      EEKDESQRT QPPPEPSAP ELDEDGYVI RPNVPHSWN 
      NEKGSFYSS SDSDTDDER ERKIHVEIK PLNNGSAPM 
      SASVDELRA TVENLYLSP TGGAYGRRG SNLDNQDLA 
      MKRSQSVSQ QLGKPSSDL LGLNFVQSP NASNSSTPT 
      SSHPYAPLQ SPSQLALNS PTLSASSRY ADLGDLLSE 
      DGEAAPGGA TKQPLRQTP TPTSGYMSI PRPPSRRSD 
      PSRHINQSN ISRADSISS LEFRTACVP VGVSRGPSP 
      LTIGMADTI PLAVAFHEI VHSYFRGAD ETRCQVKMS 
      GEMMLSFPA GIVTILANN PNPAKLVFR VKNTQRLVN 
      ILPNKQLVA IDNGQSSTD NLVLEFNMS ALSALLKKQ 
      SEQNPSASY FNVDILKYQ IKAKPGANS CPFQLVAYW 
      KCTSSHTDL KVDYKYNSH SMSSPTPLL NVTVAVPVD 
      GGVKNHQLK PAAQWLADS NRVIWKFTE LSQHSENHG 
      VGSMLARFE LENGPTNPA TISAAFNCE GTTLSGIEF 
      QLVGTGYRL SLVKRRFIS GKYICDGDL KYSSGTGTP 
      STGSAAAST VSSTGDPNC
    • >TC005182 RB
      MTVDFNDYF WGEKNNGFD VLYHNMKYG LVASKDLAE 
      LLRERSNIE ENNSKLLAK FAKQAGSCC NQGTFAPLW 
      QVLKTSAER LSALHMQMV QKVSDLVKE VNKYAEELH 
      KKHKTVKED ESGTLESVQ AMQNVTLMV QKCKDTYTQ 
      RGQELDKLR KDNASPKEI EKAELKMKK AQEEYKSYV 
      EKYTVIKDE FEKKMSVTC KNFQELEVA HLGHMKEFL 
      NSYAEVVEW THEQMGKVH KEFRQQCLE LTVDQLLEQ 
      FVRNKSTGL ERPGIVDIE EALMSPTQD SESSKPSKR 
      EGNGNSDQS VPTHAKTSR RTTSLLNLF MSNSQGSRE 
      SRAGPCSAP SSPTSPEGD QQLTRHSHR GSKWFLRSR 
      REKAKQKKS KKKVKDNVD TQSNKEEKS DMEEKDESQ 
      RTQPPPEPS APELDEDGY VIRPNVPHS WNNEKGSFY 
      SSSDSDTDD ERERKIHVE IKPLNNGSA PMSASVDEL 
      RATVENLYL SPTGGAYGR RGSNLDNQD LAMKRSQSV 
      SQQLGKPSS DLLGLNFVQ SPNASNSST PTSSHPYAP 
      LQSPSQLAL NSPTLSASS RYADLGDLL SEDGEAAPG 
      GATKQPLRQ TPTPTSGYM SIPRPPSRR SDPSRHINQ 
      SNISRADSI SSLEFRTAC VPVGVSRGP SPLTIGMAD 
      TIPLAVAFH EIVHSYFRG ADETRCQVK MSGEMMLSF 
      PAGIVTILA NNPNPAKLV FRVKNTQRL VNILPNKQL 
      VAIDNGQSS TDNLVLEFN MSALSALLK KQSEQNPSA 
      SYFNVDILK YQIKAKPGA NSCPFQLVA YWKCTSSHT 
      DLKVDYKYN SHSMSSPTP LLNVTVAVP VDGGVKNHQ 
      LKPAAQWLA DSNRVIWKF TELSQHSEN HGVGSMLAR 
      FELENGPTN PATISAAFN CEGTTLSGI EFQLVGTGY 
      RLSLVKRRF ISGKYICDG DLKYSSGTG TPSTGSAAA 
      STVSSTGDP NC
    CDS sequence
    • >TC005182 RA
      atgaccgtc gacttcaac gattatttt tggggcgag 
      aagaacaat gggttcgac gttctatac cataacatg 
      aaatatggc ctagttgct agcaaagat cttgccgaa 
      ttgctcaga gaacgatca aacattgaa gagaacaac 
      tcgaaacta cttgccaag ttcgccaag caagcagga 
      agttgttgc aatcaaggc acttttgcc ccattatgg 
      caagtcttg aagacctcg gcggagcgt ctttcggcg 
      ctccacatg caaatggtg caaaaagtg agcgacttg 
      gtcaaggaa gtgaacaaa tacgccgag gaacttcac 
      aaaaaacac aagactgtc aaggaggac gaatctggg 
      actctggag tcggtccag gccatgcag aatgtgact 
      ttaatggta caaaaatgc aaagacacc tacacccag 
      cggggacag gaattagat aagctgcgc aaagataac 
      gccagtccg aaggagata gagaaagcc gagttgaaa 
      atgaaaaaa gcgcaggag gagtacaaa tcctacgtt 
      gagaaatac acagttatc aaagacgag ttcgagaag 
      aaaatgtca gtcacttgt aagaacttc caagagctg 
      gaagttgcc catttgggc catatgaag gagttcttg 
      aattcgtac gcagaggtc gtagagtgg acacacgaa 
      caaatgggg aaggtccac aaggagttc agacagcag 
      tgtcttgag cttaccgtg gatcagttg ctcgaacag 
      ttcgttcgc aacaaatcc acgggactt gaaaggcca 
      ggtatagtt gatatagag gaggcgctg atgtcgccc 
      acgcaagac tcggagagt tcgaaacca tctaagcgc 
      gaagggttt ttacgtagt aggcgcgaa aaagctaag 
      caaaagaaa tccaaaaag aaagtcaaa gataatgta 
      gatactcaa agcaataaa gaagaaaaa tcagacatg 
      gaggaaaaa gacgaatct caacgaacc caaccacca 
      ccggagcca agtgctcct gaactggac gaggacggt 
      tacgtcata agaccaaac gttcctcat agttggaat 
      aacgaaaaa gggagtttc tactcctca tctgactcc 
      gatacagat gacgaacgc gaacgcaaa atccatgtt 
      gaaataaag cctttaaat aacggttct gcacctatg 
      tcagcttca gtagatgag ttgcgggca acagtagaa 
      aatctctat ttatcaccg actgggggc gcgtatggc 
      cgccgcggc tctaattta gacaaccaa gatctagca 
      atgaaacgt tcccaatca gtctcccaa caactcggt 
      aaacccagt agtgaccta ttgggtttg aattttgtc 
      caaagtccc aacgcatca aactcctcc actccaaca 
      agctcccat ccttacgct ccgttgcag agcccgtct 
      cagttggct ttaaactcg ccaacactt tcagcgtcc 
      tcgcgatat gctgattta ggcgacttg ttgtctgag 
      gatggtgaa gctgcacct ggaggtgca accaagcag 
      cctttgcgc caaactcca acacccact tccgggtat 
      atgtcgatt ccgagaccg ccgtctcgc cggtctgat 
      ccttcgcga catattaat caaagtaat atttcacga 
      gctgatagt attagtagt ttggaattc cgcactgct 
      tgtgttcct gtgggggtc tcaagggga ccttcgccg 
      cttaccatt ggcatggct gacacaatt ccgttagcg 
      gtcgcgttt cacgaaatt gttcattcg tacttccgg 
      ggagcagac gagaccaga tgtcaggtg aaaatgtcg 
      ggtgagatg atgttgtcg ttccctgcc ggtattgtt 
      acaatcctc gcaaataat ccgaatccg gctaaacta 
      gtctttcgg gtgaagaac acccaaagg cttgtaaat 
      atattacct aataagcaa ctagtggct attgataac 
      ggacaatca tcaactgat aatttagtc ttggaattt 
      aatatgtcc gctttaagt gcattgtta aaaaagcaa 
      tcggaacaa aaccctagc gcctcgtat ttcaatgta 
      gacatatta aaataccaa attaaagca aagcctggc 
      gcgaattcg tgcccattt caactcgtg gcctattgg 
      aaatgtaca agttcgcat acagattta aaggtggat 
      tacaaatac aattcacat tccatgagt tctcctacc 
      ccgttactc aacgtaact gtggcagtt cctgtcgat 
      ggtggtgtg aaaaaccac caattaaaa ccagcggct 
      cagtggttg gccgattca aaccgtgtg atttggaag 
      tttaccgag ttgtcccaa cattcggaa aatcatggc 
      gtcggctcg atgttagct cgtttcgaa ttagaaaac 
      ggccccact aacccggcc acgatttcc gccgcattc 
      aattgtgaa ggtaccact ctttcagga attgagttt 
      cagctggtg ggcaccggg tatcggcta tctctagtc 
      aaaaggcgg tttatatcc gggaagtat atttgtgat 
      ggcgatttg aagtatagc tcgggtacc gggacgccg 
      tccactgga tctgcggct gcgagcacg gtctcctcg 
      accggcgat ccaaactgc tag
    • >TC005182 RB
      atgaccgtc gacttcaac gattatttt tggggcgag 
      aagaacaat gggttcgac gttctatac cataacatg 
      aaatatggc ctagttgct agcaaagat cttgccgaa 
      ttgctcaga gaacgatca aacattgaa gagaacaac 
      tcgaaacta cttgccaag ttcgccaag caagcagga 
      agttgttgc aatcaaggc acttttgcc ccattatgg 
      caagtcttg aagacctcg gcggagcgt ctttcggcg 
      ctccacatg caaatggtg caaaaagtg agcgacttg 
      gtcaaggaa gtgaacaaa tacgccgag gaacttcac 
      aaaaaacac aagactgtc aaggaggac gaatctggg 
      actctggag tcggtccag gccatgcag aatgtgact 
      ttaatggta caaaaatgc aaagacacc tacacccag 
      cggggacag gaattagat aagctgcgc aaagataac 
      gccagtccg aaggagata gagaaagcc gagttgaaa 
      atgaaaaaa gcgcaggag gagtacaaa tcctacgtt 
      gagaaatac acagttatc aaagacgag ttcgagaag 
      aaaatgtca gtcacttgt aagaacttc caagagctg 
      gaagttgcc catttgggc catatgaag gagttcttg 
      aattcgtac gcagaggtc gtagagtgg acacacgaa 
      caaatgggg aaggtccac aaggagttc agacagcag 
      tgtcttgag cttaccgtg gatcagttg ctcgaacag 
      ttcgttcgc aacaaatcc acgggactt gaaaggcca 
      ggtatagtt gatatagag gaggcgctg atgtcgccc 
      acgcaagac tcggagagt tcgaaacca tctaagcgc 
      gaaggtaat ggcaatagc gaccagtcc gtgccaaca 
      catgccaaa acttcccgc cgtacaact tccctcctc 
      aatctcttc atgtctaac tcccaggga tcacgcgag 
      agccgagcc ggcccatgc agcgcgcct tcgagtccg 
      acaagtccg gaaggggac caacagctg acacgacac 
      tcccaccgt ggatctaag tggttttta cgtagtagg 
      cgcgaaaaa gctaagcaa aagaaatcc aaaaagaaa 
      gtcaaagat aatgtagat actcaaagc aataaagaa 
      gaaaaatca gacatggag gaaaaagac gaatctcaa 
      cgaacccaa ccaccaccg gagccaagt gctcctgaa 
      ctggacgag gacggttac gtcataaga ccaaacgtt 
      cctcatagt tggaataac gaaaaaggg agtttctac 
      tcctcatct gactccgat acagatgac gaacgcgaa 
      cgcaaaatc catgttgaa ataaagcct ttaaataac 
      ggttctgca cctatgtca gcttcagta gatgagttg 
      cgggcaaca gtagaaaat ctctattta tcaccgact 
      gggggcgcg tatggccgc cgcggctct aatttagac 
      aaccaagat ctagcaatg aaacgttcc caatcagtc 
      tcccaacaa ctcggtaaa cccagtagt gacctattg 
      ggtttgaat tttgtccaa agtcccaac gcatcaaac 
      tcctccact ccaacaagc tcccatcct tacgctccg 
      ttgcagagc ccgtctcag ttggcttta aactcgcca 
      acactttca gcgtcctcg cgatatgct gatttaggc 
      gacttgttg tctgaggat ggtgaagct gcacctgga 
      ggtgcaacc aagcagcct ttgcgccaa actccaaca 
      cccacttcc gggtatatg tcgattccg agaccgccg 
      tctcgccgg tctgatcct tcgcgacat attaatcaa 
      agtaatatt tcacgagct gatagtatt agtagtttg 
      gaattccgc actgcttgt gttcctgtg ggggtctca 
      aggggacct tcgccgctt accattggc atggctgac 
      acaattccg ttagcggtc gcgtttcac gaaattgtt 
      cattcgtac ttccgggga gcagacgag accagatgt 
      caggtgaaa atgtcgggt gagatgatg ttgtcgttc 
      cctgccggt attgttaca atcctcgca aataatccg 
      aatccggct aaactagtc tttcgggtg aagaacacc 
      caaaggctt gtaaatata ttacctaat aagcaacta 
      gtggctatt gataacgga caatcatca actgataat 
      ttagtcttg gaatttaat atgtccgct ttaagtgca 
      ttgttaaaa aagcaatcg gaacaaaac cctagcgcc 
      tcgtatttc aatgtagac atattaaaa taccaaatt 
      aaagcaaag cctggcgcg aattcgtgc ccatttcaa 
      ctcgtggcc tattggaaa tgtacaagt tcgcataca 
      gatttaaag gtggattac aaatacaat tcacattcc 
      atgagttct cctaccccg ttactcaac gtaactgtg 
      gcagttcct gtcgatggt ggtgtgaaa aaccaccaa 
      ttaaaacca gcggctcag tggttggcc gattcaaac 
      cgtgtgatt tggaagttt accgagttg tcccaacat 
      tcggaaaat catggcgtc ggctcgatg ttagctcgt 
      ttcgaatta gaaaacggc cccactaac ccggccacg 
      atttccgcc gcattcaat tgtgaaggt accactctt 
      tcaggaatt gagtttcag ctggtgggc accgggtat 
      cggctatct ctagtcaaa aggcggttt atatccggg 
      aagtatatt tgtgatggc gatttgaag tatagctcg 
      ggtaccggg acgccgtcc actggatct gcggctgcg 
      agcacggtc tcctcgacc ggcgatcca aactgctag
    mRNA sequence
    • >TC005182 RA
      cggctgtat caattctgt gatgatccg ccccctacg 
      atctcgtta tcgcaatta aataccgtc aaacgggtg 
      aatgtcgtt aaatgttga atggcagat taagttaag 
      gggttgatt tttgttaat ttcgattgg gaattagtt 
      gtttatgtt aaatgttgt ttgttgtgt gtaattagc 
      gttgtctgg ttgagagga agtcgtaaa cgaatgacg 
      gttgtgatt gtttatatt cgccgtata ctaatcaga 
      tcatttggc atggttata gtgcaacaa tgaccgtcg 
      acttcaacg attattttt ggggcgaga agaacaatg 
      ggttcgacg ttctatacc ataacatga aatatggcc 
      tagttgcta gcaaagatc ttgccgaat tgctcagag 
      aacgatcaa acattgaag agaacaact cgaaactac 
      ttgccaagt tcgccaagc aagcaggaa gttgttgca 
      atcaaggca cttttgccc cattatggc aagtcttga 
      agacctcgg cggagcgtc tttcggcgc tccacatgc 
      aaatggtgc aaaaagtga gcgacttgg tcaaggaag 
      tgaacaaat acgccgagg aacttcaca aaaaacaca 
      agactgtca aggaggacg aatctggga ctctggagt 
      cggtccagg ccatgcaga atgtgactt taatggtac 
      aaaaatgca aagacacct acacccagc ggggacagg 
      aattagata agctgcgca aagataacg ccagtccga 
      aggagatag agaaagccg agttgaaaa tgaaaaaag 
      cgcaggagg agtacaaat cctacgttg agaaataca 
      cagttatca aagacgagt tcgagaaga aaatgtcag 
      tcacttgta agaacttcc aagagctgg aagttgccc 
      atttgggcc atatgaagg agttcttga attcgtacg 
      cagaggtcg tagagtgga cacacgaac aaatgggga 
      aggtccaca aggagttca gacagcagt gtcttgagc 
      ttaccgtgg atcagttgc tcgaacagt tcgttcgca 
      acaaatcca cgggacttg aaaggccag gtatagttg 
      atatagagg aggcgctga tgtcgccca cgcaagact 
      cggagagtt cgaaaccat ctaagcgcg aagggtttt 
      tacgtagta ggcgcgaaa aagctaagc aaaagaaat 
      ccaaaaaga aagtcaaag ataatgtag atactcaaa 
      gcaataaag aagaaaaat cagacatgg aggaaaaag 
      acgaatctc aacgaaccc aaccaccac cggagccaa 
      gtgctcctg aactggacg aggacggtt acgtcataa 
      gaccaaacg ttcctcata gttggaata acgaaaaag 
      ggagtttct actcctcat ctgactccg atacagatg 
      acgaacgcg aacgcaaaa tccatgttg aaataaagc 
      ctttaaata acggttctg cacctatgt cagcttcag 
      tagatgagt tgcgggcaa cagtagaaa atctctatt 
      tatcaccga ctgggggcg cgtatggcc gccgcggct 
      ctaatttag acaaccaag atctagcaa tgaaacgtt 
      cccaatcag tctcccaac aactcggta aacccagta 
      gtgacctat tgggtttga attttgtcc aaagtccca 
      acgcatcaa actcctcca ctccaacaa gctcccatc 
      cttacgctc cgttgcaga gcccgtctc agttggctt 
      taaactcgc caacacttt cagcgtcct cgcgatatg 
      ctgatttag gcgacttgt tgtctgagg atggtgaag 
      ctgcacctg gaggtgcaa ccaagcagc ctttgcgcc 
      aaactccaa cacccactt ccgggtata tgtcgattc 
      cgagaccgc cgtctcgcc ggtctgatc cttcgcgac 
      atattaatc aaagtaata tttcacgag ctgatagta 
      ttagtagtt tggaattcc gcactgctt gtgttcctg 
      tgggggtct caaggggac cttcgccgc ttaccattg 
      gcatggctg acacaattc cgttagcgg tcgcgtttc 
      acgaaattg ttcattcgt acttccggg gagcagacg 
      agaccagat gtcaggtga aaatgtcgg gtgagatga 
      tgttgtcgt tccctgccg gtattgtta caatcctcg 
      caaataatc cgaatccgg ctaaactag tctttcggg 
      tgaagaaca cccaaaggc ttgtaaata tattaccta 
      ataagcaac tagtggcta ttgataacg gacaatcat 
      caactgata atttagtct tggaattta atatgtccg 
      ctttaagtg cattgttaa aaaagcaat cggaacaaa 
      accctagcg cctcgtatt tcaatgtag acatattaa 
      aataccaaa ttaaagcaa agcctggcg cgaattcgt 
      gcccatttc aactcgtgg cctattgga aatgtacaa 
      gttcgcata cagatttaa aggtggatt acaaataca 
      attcacatt ccatgagtt ctcctaccc cgttactca 
      acgtaactg tggcagttc ctgtcgatg gtggtgtga 
      aaaaccacc aattaaaac cagcggctc agtggttgg 
      ccgattcaa accgtgtga tttggaagt ttaccgagt 
      tgtcccaac attcggaaa atcatggcg tcggctcga 
      tgttagctc gtttcgaat tagaaaacg gccccacta 
      acccggcca cgatttccg ccgcattca attgtgaag 
      gtaccactc tttcaggaa ttgagtttc agctggtgg 
      gcaccgggt atcggctat ctctagtca aaaggcggt 
      ttatatccg ggaagtata tttgtgatg gcgatttga 
      agtatagct cgggtaccg ggacgccgt ccactggat 
      ctgcggctg cgagcacgg tctcctcga ccggcgatc 
      caaactgct agcattccc acatatcac acgtgcaat 
      atgtgcata tataacttt taaatttgt atgtcaact 
      atttttatt tatgactat ttccatgtt atatagcaa 
      atattatat atagtttat gaagctatt ttataatca 
      ataaagatt ttgtaaagg aattactgc ttttttatt 
      gtttgagtc agccgagtc gttgtgacc caagttgcg 
      ctctcgggc tagaaagag ttcaaattc agttccaaa 
      aaggcacta aatccttat ttttgtttt gtaccaatt 
      tctgtttat tagaaaaat aaccgtatc ttatcttag 
      ataattaca ctattttct tagggtagt aagtttagt 
      tttttgttt gatgtttca cgttcggta aataaaaca 
      tttttcatt ta
    • >TC005182 RB
      cggctgtat caattctgt gatgatccg ccccctacg 
      atctcgtta tcgcaatta aataccgtc aaacgggtg 
      aatgtcgtt aaatgttga atggcagat taagttaag 
      gggttgatt tttgttaat ttcgattgg gaattagtt 
      gtttatgtt aaatgttgt ttgttgtgt gtaattagc 
      gttgtctgg ttgagagga agtcgtaaa cgaatgacg 
      gttgtgatt gtttatatt cgccgtata ctaatcaga 
      tcatttggc atggttata gtgcaacaa tgaccgtcg 
      acttcaacg attattttt ggggcgaga agaacaatg 
      ggttcgacg ttctatacc ataacatga aatatggcc 
      tagttgcta gcaaagatc ttgccgaat tgctcagag 
      aacgatcaa acattgaag agaacaact cgaaactac 
      ttgccaagt tcgccaagc aagcaggaa gttgttgca 
      atcaaggca cttttgccc cattatggc aagtcttga 
      agacctcgg cggagcgtc tttcggcgc tccacatgc 
      aaatggtgc aaaaagtga gcgacttgg tcaaggaag 
      tgaacaaat acgccgagg aacttcaca aaaaacaca 
      agactgtca aggaggacg aatctggga ctctggagt 
      cggtccagg ccatgcaga atgtgactt taatggtac 
      aaaaatgca aagacacct acacccagc ggggacagg 
      aattagata agctgcgca aagataacg ccagtccga 
      aggagatag agaaagccg agttgaaaa tgaaaaaag 
      cgcaggagg agtacaaat cctacgttg agaaataca 
      cagttatca aagacgagt tcgagaaga aaatgtcag 
      tcacttgta agaacttcc aagagctgg aagttgccc 
      atttgggcc atatgaagg agttcttga attcgtacg 
      cagaggtcg tagagtgga cacacgaac aaatgggga 
      aggtccaca aggagttca gacagcagt gtcttgagc 
      ttaccgtgg atcagttgc tcgaacagt tcgttcgca 
      acaaatcca cgggacttg aaaggccag gtatagttg 
      atatagagg aggcgctga tgtcgccca cgcaagact 
      cggagagtt cgaaaccat ctaagcgcg aaggtaatg 
      gcaatagcg accagtccg tgccaacac atgccaaaa 
      cttcccgcc gtacaactt ccctcctca atctcttca 
      tgtctaact cccagggat cacgcgaga gccgagccg 
      gcccatgca gcgcgcctt cgagtccga caagtccgg 
      aaggggacc aacagctga cacgacact cccaccgtg 
      gatctaagt ggtttttac gtagtaggc gcgaaaaag 
      ctaagcaaa agaaatcca aaaagaaag tcaaagata 
      atgtagata ctcaaagca ataaagaag aaaaatcag 
      acatggagg aaaaagacg aatctcaac gaacccaac 
      caccaccgg agccaagtg ctcctgaac tggacgagg 
      acggttacg tcataagac caaacgttc ctcatagtt 
      ggaataacg aaaaaggga gtttctact cctcatctg 
      actccgata cagatgacg aacgcgaac gcaaaatcc 
      atgttgaaa taaagcctt taaataacg gttctgcac 
      ctatgtcag cttcagtag atgagttgc gggcaacag 
      tagaaaatc tctatttat caccgactg ggggcgcgt 
      atggccgcc gcggctcta atttagaca accaagatc 
      tagcaatga aacgttccc aatcagtct cccaacaac 
      tcggtaaac ccagtagtg acctattgg gtttgaatt 
      ttgtccaaa gtcccaacg catcaaact cctccactc 
      caacaagct cccatcctt acgctccgt tgcagagcc 
      cgtctcagt tggctttaa actcgccaa cactttcag 
      cgtcctcgc gatatgctg atttaggcg acttgttgt 
      ctgaggatg gtgaagctg cacctggag gtgcaacca 
      agcagcctt tgcgccaaa ctccaacac ccacttccg 
      ggtatatgt cgattccga gaccgccgt ctcgccggt 
      ctgatcctt cgcgacata ttaatcaaa gtaatattt 
      cacgagctg atagtatta gtagtttgg aattccgca 
      ctgcttgtg ttcctgtgg gggtctcaa ggggacctt 
      cgccgctta ccattggca tggctgaca caattccgt 
      tagcggtcg cgtttcacg aaattgttc attcgtact 
      tccggggag cagacgaga ccagatgtc aggtgaaaa 
      tgtcgggtg agatgatgt tgtcgttcc ctgccggta 
      ttgttacaa tcctcgcaa ataatccga atccggcta 
      aactagtct ttcgggtga agaacaccc aaaggcttg 
      taaatatat tacctaata agcaactag tggctattg 
      ataacggac aatcatcaa ctgataatt tagtcttgg 
      aatttaata tgtccgctt taagtgcat tgttaaaaa 
      agcaatcgg aacaaaacc ctagcgcct cgtatttca 
      atgtagaca tattaaaat accaaatta aagcaaagc 
      ctggcgcga attcgtgcc catttcaac tcgtggcct 
      attggaaat gtacaagtt cgcatacag atttaaagg 
      tggattaca aatacaatt cacattcca tgagttctc 
      ctaccccgt tactcaacg taactgtgg cagttcctg 
      tcgatggtg gtgtgaaaa accaccaat taaaaccag 
      cggctcagt ggttggccg attcaaacc gtgtgattt 
      ggaagttta ccgagttgt cccaacatt cggaaaatc 
      atggcgtcg gctcgatgt tagctcgtt tcgaattag 
      aaaacggcc ccactaacc cggccacga tttccgccg 
      cattcaatt gtgaaggta ccactcttt caggaattg 
      agtttcagc tggtgggca ccgggtatc ggctatctc 
      tagtcaaaa ggcggttta tatccggga agtatattt 
      gtgatggcg atttgaagt atagctcgg gtaccggga 
      cgccgtcca ctggatctg cggctgcga gcacggtct 
      cctcgaccg gcgatccaa actgctagc attcccaca 
      tatcacacg tgcaatatg tgcatatat aacttttaa 
      atttgtatg tcaactatt tttatttat gactatttc 
      catgttata tagcaaata ttatatata gtttatgaa 
      gctatttta taatcaata aagattttg taaaggaat 
      tactgcttt tttattgtt tgagtcagc cgagtcgtt 
      gtgacccaa gttgcgctc tcgggctag aaagagttc 
      aaattcagt tccaaaaag gcactaaat ccttatttt 
      tgttttgta ccaatttct gtttattag aaaaataac 
      cgtatctta tcttagata attacacta ttttcttag 
      ggtagtaag tttagtttt ttgtttgat gtttcacgt 
      tcggtaaat aaaacattt ttcattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY