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Gene information
General information
TC014515 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6417225..6418772
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014515
    iBeetle screen iB_10687

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014515 RA
      MRLSRHLCK QHIGWHLKK LWANQSIKK IEDSQAEKR 
      LLEKGIPVV RAEDVLQES YREQHKFEK VEVVGFKPP 
      PIAFDNTHP NWHDRVLLT YKDNNVLLE GLKQAKILT 
      NTVELTEGL PKSRAIESN KEIERRVKE IILSSHVFD 
      AEQQKLPKL KDPERPAWN FPRVYGITQ PRVIKLLLS 
      NLLQLIENT SDLELVKNR FVIDDAHFS YSFEKYGKL 
      MQFELKGDT LLTSKSPLS PVTDQPTKE LELPDIFPI 
      EPTITLNEE NVYTVRQIY PINIQFAKS HPHTIFIPH 
      VETDVKNIF EEPVTDEQI FGRSLLKTF TVAASYAKE 
      KFGDVKILP KPVTVQCVH TNGHLFHFG VFQLNTLDL 
      EDSGVKNVW YQTNRIPLF ESCGYKVGK PVLEGYNRE 
      VMHILTSFY NNV
    CDS sequence
    • >TC014515 RA
      atgcggctt tctcggcac ctctgcaag cagcacatc 
      ggttggcac ctgaaaaaa ctgtgggcg aaccaaagc 
      attaaaaaa atcgaggat tcacaagct gagaaaagg 
      ttgttagag aaaggcatt ccggtagtt cgtgccgaa 
      gatgtcttg caagagagc tacagggaa caacacaaa 
      ttcgaaaaa gttgaggtt gtggggttt aagccgcca 
      ccaattgct tttgataac actcaccca aattggcac 
      gacagggtt ttgctcaca tataaagac aacaatgtg 
      cttttggag ggtcttaaa caggccaag attttaaca 
      aacaccgtg gaacttacg gaaggttta ccgaaaagt 
      agagcaatt gagagtaat aaggaaatt gaaaggaga 
      gtcaaggag attattctt tcgtcacac gtgtttgat 
      gcagagcag caaaaactg ccaaagttg aaggacccg 
      gagagaccg gcttggaat tttccccgg gtttatggc 
      attacgcag ccccgagtc attaagtta ctgttatca 
      aacttattg caactaatt gaaaacacg agtgacttg 
      gagttggtt aaaaaccgg tttgtaatt gacgacgca 
      catttctcg tacagtttt gaaaagtat ggcaaattg 
      atgcagttt gaattaaaa ggggacacc cttttaact 
      tcaaaatcg ccgttaagt cctgttact gaccaacca 
      accaaagag ctcgaacta cctgatatt ttcccaata 
      gaacccacc ataacacta aatgaggaa aatgtttac 
      acagttaga caaatttat cctataaac attcaattt 
      gccaaatcg caccctcac acaatattt ataccgcat 
      gtggaaacc gacgtaaaa aacatattc gaggaacct 
      gtaacagac gagcaaatt tttggacgc tcccttttg 
      aagaccttc acagtggct gcgtcatat gccaaggaa 
      aaatttggg gatgttaaa attttgccc aagcctgtg 
      acagtccag tgtgtgcac accaacggc cacttgttc 
      cactttggg gtgtttcag ttgaacaca cttgacttg 
      gaagacagt ggggtgaaa aatgtctgg taccagacc 
      aacagaatc ccgcttttt gagagttgt ggctacaaa 
      gtcggcaaa ccggtcttg gagggctac aaccgcgaa 
      gtaatgcat attttaaca agtttttat aataacgtt 
      taa
    mRNA sequence
    • >TC014515 RA
      caaattgag gttatgaag tgtcaaatt tgaggttaa 
      gttttcgtg aagtaaggt tatcttggc gcaatcagt 
      gaactaaaa tgcggcttt ctcggcacc tctgcaagc 
      agcacatcg gttggcacc tgaaaaaac tgtgggcga 
      accaaagca ttaaaaaaa tcgaggatt cacaagctg 
      agaaaaggt tgttagaga aaggcattc cggtagttc 
      gtgccgaag atgtcttgc aagagagct acagggaac 
      aacacaaat tcgaaaaag ttgaggttg tggggttta 
      agccgccac caattgctt ttgataaca ctcacccaa 
      attggcacg acagggttt tgctcacat ataaagaca 
      acaatgtgc ttttggagg gtcttaaac aggccaaga 
      ttttaacaa acaccgtgg aacttacgg aaggtttac 
      cgaaaagta gagcaattg agagtaata aggaaattg 
      aaaggagag tcaaggaga ttattcttt cgtcacacg 
      tgtttgatg cagagcagc aaaaactgc caaagttga 
      aggacccgg agagaccgg cttggaatt ttccccggg 
      tttatggca ttacgcagc cccgagtca ttaagttac 
      tgttatcaa acttattgc aactaattg aaaacacga 
      gtgacttgg agttggtta aaaaccggt ttgtaattg 
      acgacgcac atttctcgt acagttttg aaaagtatg 
      gcaaattga tgcagtttg aattaaaag gggacaccc 
      ttttaactt caaaatcgc cgttaagtc ctgttactg 
      accaaccaa ccaaagagc tcgaactac ctgatattt 
      tcccaatag aacccacca taacactaa atgaggaaa 
      atgtttaca cagttagac aaatttatc ctataaaca 
      ttcaatttg ccaaatcgc accctcaca caatattta 
      taccgcatg tggaaaccg acgtaaaaa acatattcg 
      aggaacctg taacagacg agcaaattt ttggacgct 
      cccttttga agaccttca cagtggctg cgtcatatg 
      ccaaggaaa aatttgggg atgttaaaa ttttgccca 
      agcctgtga cagtccagt gtgtgcaca ccaacggcc 
      acttgttcc actttgggg tgtttcagt tgaacacac 
      ttgacttgg aagacagtg gggtgaaaa atgtctggt 
      accagacca acagaatcc cgctttttg agagttgtg 
      gctacaaag tcggcaaac cggtcttgg agggctaca 
      accgcgaag taatgcata ttttaacaa gtttttata 
      ataacgttt aattactat aaaatgtac atataaaaa 
      ttgcaatta tcacataaa acattgata gaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY