Status
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Gene information
General information
TC001226 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12976279..12980406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001226
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10686

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001226 RA
        MNSALKPVL TSSTPQNPP VTTQLNKPM HPQGAPANY 
        GQPSTHMPQ SLVNLPHNP TVAHTHPNP AHQSNHYSL 
        NVPNAPHYS SNNPSTIPA SNHPVPNVS QGQGGQTYS 
        PNKNVPFSG GYQPNLGHP PNQNPMPPV SQTLPATQP 
        HQPPAAADE KPQSNGTAE AKSPTKPEP QENHVSLPR 
        DKSPMPPSS PKTTEKPEE KPSPTALPE QKQPQESKP 
        VEEAKTSPE PADSPPESA PAVSDTDPK PETPDTPEA 
        KPSEPQDTK ENITEEATQ SPPPKTKEQ KPKTTPKTA 
        RKSRTPKPE GDSPKTSKA QQKSPTVAK SKRTRTKTQ 
        PYQSPLPEI EIITKITSS TPRNKTNDD KLIVFYKNE 
        FLAVRNSEG SFYICQAAQ NIYKSSPKI RIRWLSQEK 
        SEKSGEVYT PDFYDNTDF DCILTNLNL VRVDKGRYR 
        LPPAEKERT DSILKRSLA VEKGEVTSP SLTEEHPDG 
        IDVSLYKEE SQLKKRKST KRKASSALR SKSASSTTK 
        SPDQPKVRK VVKPVKKTP KKAPAPAKT KPKPEEKKP 
        IASSSRTDR AKRRSDNQN HNKVTPVVD QKKAKVLAK 
        VARKGAPKA GRNAKVAKA KKAAPKVSA KKTKRSKK
      CDS sequence
      • >TC001226 RA
        atgaattca gctttgaag ccagttttg acgtcttcg 
        acgccacag aatcctcct gtgacgact cagctaaac 
        aaaccgatg catcctcaa ggagcccca gctaattat 
        gggcaacca tcaactcac atgcctcag agtttagtt 
        aatttaccc cataaccca acggtcgcc cacacacat 
        cccaatcct gcccatcag agtaaccat tactcgtta 
        aatgtgccc aacgcgcca cattactcc agcaacaac 
        ccatcgacg atacctgcc agcaaccat ccagtaccc 
        aacgtcagt caagggcaa ggcggacag acctattca 
        cccaacaaa aatgtacca ttttcggga ggataccaa 
        ccaaacctc ggacatccg cccaatcaa aatccgatg 
        cccccggtg agccagacc ctacccgcc acgcagccc 
        caccagccc cccgcggcc gccgacgag aagccccaa 
        tcgaacggc accgccgaa gcgaaatcg ccgacgaag 
        ccggagccc caagagaac cacgtgtct ttaccgcgg 
        gacaaatcg ccgatgccc cccagttcg cccaaaacg 
        acggaaaaa cccgaagaa aaaccctcc ccgacggcc 
        ttacctgaa cagaaacaa cctcaggaa tcgaaaccg 
        gtggaagaa gctaaaacg tcccccgag ccggccgac 
        agcccccct gaaagtgcg ccggccgtg agtgacact 
        gacccgaaa cctgaaaca cccgacact cccgaggcg 
        aagccgagt gagccacaa gacactaaa gaaaacatc 
        acggaggag gcgacgcaa agcccgccc cccaaaaca 
        aaagaacaa aagcccaaa accacgccc aaaacggcg 
        agaaaatca cgaacgccc aaaccagag ggggattca 
        cccaaaacg tccaaggcc cagcagaaa tcaccgacg 
        gttgctaaa agcaaaagg acgcgaacc aagacacaa 
        ccttatcaa agtccatta cctgaaatt gaaattatc 
        actaaaatt acgtcgtca actcccagg aataaaaca 
        aatgacgat aaattgatt gttttctat aaaaacgaa 
        tttttggct gtaagaaac tcagagggt agtttttat 
        atttgtcaa gctgcgcaa aatatctat aaaagctca 
        ccaaaaatc cggattcgt tggctttcg caggagaag 
        agcgaaaag tccggcgag gtgtacaca cctgatttt 
        tacgataat acagatttt gattgtata ttaacaaac 
        ctaaatttg gtgcgcgtc gataagggt cgttacaga 
        ttgcccccc gccgaaaaa gagcgaacc gacagtatt 
        ttgaagcgg tctctggct gtggaaaag ggcgaagtg 
        acctcgccc tccctcaca gaagagcat ccagatggc 
        atagacgtg tcactttac aaggaggag agccagttg 
        aagaaacga aaatcgacc aaacgcaag gcctcctcc 
        gccctccgc tcaaagtcc gccagcagt acgacaaag 
        tcgcccgat caaccgaaa gtccgcaaa gtggtcaag 
        ccggtgaaa aaaacaccc aagaaggca ccagccccc 
        gctaaaact aagccgaag cctgaggag aaaaaaccg 
        attgcgtcg tcaagtcgg acagatcgg gcgaaacgg 
        cgaagcgac aaccagaat cacaataaa gtcacgccg 
        gtggttgat cagaagaag gccaaggtg ctggccaaa 
        gtggccagg aaaggcgcc cccaaggcg ggcagaaat 
        gcgaaagtg gcaaaggcc aagaaggcg gcgcctaag 
        gtcagcgcg aagaaaaca aagcggtcg aagaaataa
      mRNA sequence
      • >TC001226 RA
        tagttaaaa agagataga aacatggag aaatgaccg 
        acaagcgac aacagagcg gcaagagct aaacctacg 
        gcacggcga cagcgacgc cataagtat cccgaaagt 
        ttgtgctta atttcatcg aaaacaatg cgtacaaac 
        ttgtggttg ttgaatttg tgaaaagat gaattcagc 
        tttgaagcc agttttgac gtcttcgac gccacagaa 
        tcctcctgt gacgactca gctaaacaa accgatgca 
        tcctcaagg agccccagc taattatgg gcaaccatc 
        aactcacat gcctcagag tttagttaa tttacccca 
        taacccaac ggtcgccca cacacatcc caatcctgc 
        ccatcagag taaccatta ctcgttaaa tgtgcccaa 
        cgcgccaca ttactccag caacaaccc atcgacgat 
        acctgccag caaccatcc agtacccaa cgtcagtca 
        agggcaagg cggacagac ctattcacc caacaaaaa 
        tgtaccatt ttcgggagg ataccaacc aaacctcgg 
        acatccgcc caatcaaaa tccgatgcc cccggtgag 
        ccagaccct acccgccac gcagcccca ccagccccc 
        cgcggccgc cgacgagaa gccccaatc gaacggcac 
        cgccgaagc gaaatcgcc gacgaagcc ggagcccca 
        agagaacca cgtgtcttt accgcggga caaatcgcc 
        gatgccccc cagttcgcc caaaacgac ggaaaaacc 
        cgaagaaaa accctcccc gacggcctt acctgaaca 
        gaaacaacc tcaggaatc gaaaccggt ggaagaagc 
        taaaacgtc ccccgagcc ggccgacag cccccctga 
        aagtgcgcc ggccgtgag tgacactga cccgaaacc 
        tgaaacacc cgacactcc cgaggcgaa gccgagtga 
        gccacaaga cactaaaga aaacatcac ggaggaggc 
        gacgcaaag cccgccccc caaaacaaa agaacaaaa 
        gcccaaaac cacgcccaa aacggcgag aaaatcacg 
        aacgcccaa accagaggg ggattcacc caaaacgtc 
        caaggccca gcagaaatc accgacggt tgctaaaag 
        caaaaggac gcgaaccaa gacacaacc ttatcaaag 
        tccattacc tgaaattga aattatcac taaaattac 
        gtcgtcaac tcccaggaa taaaacaaa tgacgataa 
        attgattgt tttctataa aaacgaatt tttggctgt 
        aagaaactc agagggtag tttttatat ttgtcaagc 
        tgcgcaaaa tatctataa aagctcacc aaaaatccg 
        gattcgttg gctttcgca ggagaagag cgaaaagtc 
        cggcgaggt gtacacacc tgattttta cgataatac 
        agattttga ttgtatatt aacaaacct aaatttggt 
        gcgcgtcga taagggtcg ttacagatt gccccccgc 
        cgaaaaaga gcgaaccga cagtatttt gaagcggtc 
        tctggctgt ggaaaaggg cgaagtgac ctcgccctc 
        cctcacaga agagcatcc agatggcat agacgtgtc 
        actttacaa ggaggagag ccagttgaa gaaacgaaa 
        atcgaccaa acgcaaggc ctcctccgc cctccgctc 
        aaagtccgc cagcagtac gacaaagtc gcccgatca 
        accgaaagt ccgcaaagt ggtcaagcc ggtgaaaaa 
        aacacccaa gaaggcacc agcccccgc taaaactaa 
        gccgaagcc tgaggagaa aaaaccgat tgcgtcgtc 
        aagtcggac agatcgggc gaaacggcg aagcgacaa 
        ccagaatca caataaagt cacgccggt ggttgatca 
        gaagaaggc caaggtgct ggccaaagt ggccaggaa 
        aggcgcccc caaggcggg cagaaatgc gaaagtggc 
        aaaggccaa gaaggcggc gcctaaggt cagcgcgaa 
        gaaaacaaa gcggtcgaa gaaataaag tatgtatat 
        tcacatcga acttatcac attttcact gagagttgt 
        gatgtgtga gttgtgatg aagaggaat tgcgtccaa 
        tacatattt ctgacaaat caaactgat acgtagcca 
        gagagaaaa tgatgaact tgcagttta ggccttaac 
        ttttagtta ttatggaat gtgtgtttg gatgacatt 
        cttcgtatg ttgcaatgt tgatagttc tattatcta 
        ttggccatc ttcagtaac ggtatttat ttttgtgtc 
        atttttggt agagttctg attttttgt ttaagagtg 
        tttataaca ataatttaa gggcgtaat attctcttc 
        ttcaaatgc gttatttaa gttttctca attataaag 
        tctaaatag cactagtat tacttatat tttgtatcg 
        gtactcatt tgtacattt aaagctcta cttagatgt 
        aaagtcatg tgagcatat aatatagat ttaatctat 
        gaataaaat tgtttcaat gatacttag gcgaccgtt 
        gatgagtat atgtaaata tacaatgac caatataac 
        tatatattt ctttataat taacaaaag cacaggcaa 
        ttttcgatt aagttatta ggagttagt atgtgttgt 
        gcttaaaca aattaagta ttaaagaga tgtggaatg 
        gatgttgct gtagtcttg tgccagtcg aaattcgat 
        gaaagttaa attttcctg aaataattt ataaatgga 
        aataaatta ttgaataaa tc
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY