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Gene information
General information
TC003203 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 298334..304347
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003203
    iBeetle screen iB_10680

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003203 RA
      MTGTIAATN GDAKQGVQL KRELGLFSA INLILAVMI 
      GSGIFVSPA SALEHSGSV GMCLVVWTV CGIISLLGA 
      LAFAELGTV IPRSGAEYA YYMDSFGPL HKFWGHLPS 
      FIYSWVMIV IIKPAEVAV IILTFSEYL CQPLLDLMC 
      IQDDSEEVK KAVKTVALL ALGIITYIN VSSVKLYVK 
      VQNIFGGFK VFACLVVIF GGLYEIIKG NTDNLNRGF 
      EGTKYTPKS IALAFYSGL WAYDGWSAV TVITEEIKR 
      PEVNIPRSI MISVPIVTG LYVFMNMAY MTALSIPEM 
      INSPAVAVT FGERVLGPL AFLIPLGVA LATFGCALS 
      IQFGVTRVC YVAGQDGLM IKSLSFVHH ERLTPSFAV 
      VSQGILSLL FIVAGDIVE LIEFVSFLI WIFYGLAFV 
      SLLVLRRTM KDAPRPYKV PTWICVFVL LVAIYLSVT 
      PIITDPTPK YLLALAFIL VGVLVYYWF VYKKRTPEL 
      LMRKFTRFI QILFAAVPP DNPNAT
    CDS sequence
    • >TC003203 RA
      atgaccggg accatagcg gccacaaat ggcgacgcc 
      aaacaaggc gtccaactc aagcgcgaa ttgggcctt 
      ttttccgcc ataaatcta attcttgcc gtcatgata 
      ggatccgga attttcgtc tctccagct tcagcactc 
      gaacattcc ggttcagtt ggaatgtgt ctagttgta 
      tggacagta tgcggaata atctccctt ctaggagct 
      ctggctttt gccgaacta ggcactgtt atcccacgt 
      tcaggggcc gaatacgcc tactatatg gactcgttt 
      ggccccttg cacaaattc tggggccat ttgccctca 
      ttcatctac tcctgggtc atgattgtg ataatcaaa 
      ccggcagaa gtcgctgtt attatactg actttttcc 
      gaatatttg tgccaaccg ttgctagat ctgatgtgc 
      atacaggac gactctgaa gaggtgaag aaggcggtc 
      aaaacggtg gccttactc gccctaggt ataataaca 
      tacatcaac gtttcaagc gtgaaactc tacgtcaaa 
      gtgcagaac attttcggc ggatttaaa gtattcgcc 
      tgtttggtg gtgattttt ggcggcctt tacgaaatc 
      atcaaaggc aacactgac aatttaaac cggggcttc 
      gaaggcaca aagtacaca ccgaaaagc atcgctttg 
      gccttttat agcggactt tgggcttat gacggatgg 
      tctgcggtc actgtaatc actgaagag atcaaacga 
      cctgaagta aacatcccg aggagcata atgataagt 
      gttcccata gtcaccggt ttgtatgtc tttatgaac 
      atggcatac atgaccgca ttatcgata cctgagatg 
      ataaattcg cctgctgtt gctgtaact ttcggtgaa 
      agggtttta ggccctttg gcgtttttg ataccacta 
      ggagtggcg ttagctacg tttggatgt gccctaagt 
      atacaattt ggggtcact agggtgtgt tatgtggca 
      ggacaggac ggcctaatg atcaaaagt ctttccttc 
      gttcatcat gaacgtcta actccgtca ttcgctgtc 
      gtttctcag gggatttta tcgctcctt tttatcgta 
      gctggggat attgttgaa ctgatcgaa tttgttagt 
      ttcctcatt tggatattc tacgggctg gcttttgtc 
      tcgttactt gtcttaagg aggactatg aaagatgct 
      cctcgaccc tacaaagtg cctacatgg atttgtgtt 
      ttcgtttta ttggtagct atttatttg agtgttacg 
      cccattatt acggatcca actccgaaa tatttgctc 
      gctttggcg tttattttg gtgggggtt ttggtttat 
      tattggttt gtttacaag aagcgaaca ccggaattg 
      ttgatgaga aagtttaca cgttttatt cagatttta 
      ttcgcggct gtgccaccc gataatcca aatgctaca 
      taa
    mRNA sequence
    • >TC003203 RA
      ttcaaagct tacaaaaca tttcaagat tatttcgtt 
      tttcgataa aatacatga cgaaaaaca ctgacctga 
      ggtagcgtc cctcttgct tttggcgcg ttctcgagc 
      atttctcag aattcggtt ccagactgc aaccgatga 
      cgtcacctc gtcagattt ccaataaaa accttggat 
      ttcagtcat tttggcaca aaacacaca cacaacact 
      tttgtacac accacaaaa tcaaaaaac ttgaggacg 
      ctctgacgt gaaaatgcg tccccacca ctctaatgc 
      gtgacatca tggcacgac gtctcattg gttgacgcc 
      gttgacgta tgaccgaca ggcgcgcga tttgaaatt 
      tgtgttctc tggatttat caatatttg acgtcgatt 
      ttcgactgt ttcttagtc acaaccttt ccaattaac 
      cgaatttct caaaaacga gccataaaa tcgttcatt 
      tcacaggtt atgaaataa ttttcggga aaaaaacat 
      tataaatta aaaaaaaaa tagacaaaa attcgaaag 
      cactattta ttatatttt gtgtgacag tgttggaaa 
      caaatttgt ttgccccat tattatttt aaaatcgac 
      tctatcgat aaaacttcc acttgattt attttttac 
      acattttaa gaccccagt tacagataa gccgatgaa 
      tgatgattc cttatcatt gcgaaaaaa tcgatatta 
      caattcatc tcacacaac acttgacct taataatta 
      cttaattta ttagttact agagtgcaa gtcaatgca 
      aaaggaagc ctcgaatta ccactctct ttatataat 
      aattacgcc aattaccct tcacaatca ccacacata 
      atattttgg gaaaatttc aactcgtcc attgcttaa 
      catcagatg tttatagga gtcatcagt atttgttta 
      ctaatcacc acattgttt aaatttcga tattgcaca 
      cgatattga acagacaac ataattaac attatcgct 
      tttattact atatgtatt acaaatcgg agatttttg 
      gagtaattt gtgcgcgta ttggtcata attttcacg 
      cattattgt acaagcaat tttcttatt tgttcagac 
      gttgtagtg tgtagtagc gctcaactt gaaattgtt 
      gctatctct tatcctctt taaactaaa caagagatt 
      actgatacg atttcgata tctgtaatt taaaattaa 
      tgtgaatga acggttgtc agtgctcac aatagttta 
      ttcgaatct ggtgaacgc acaccacac acttcttca 
      gtattaaag caatgcagt aatttcaat ttttcgttt 
      ttcaagtgt aatttcatt aaaaatgac cgggaccat 
      agcggccac aaatggcga cgccaaaca aggcgtcca 
      actcaagcg cgaattggg ccttttttc cgccataaa 
      tctaattct tgccgtcat gataggatc cggaatttt 
      cgtctctcc agcttcagc actcgaaca ttccggttc 
      agttggaat gtgtctagt tgtatggac agtatgcgg 
      aataatctc ccttctagg agctctggc ttttgccga 
      actaggcac tgttatccc acgttcagg ggccgaata 
      cgcctacta tatggactc gtttggccc cttgcacaa 
      attctgggg ccatttgcc ctcattcat ctactcctg 
      ggtcatgat tgtgataat caaaccggc agaagtcgc 
      tgttattat actgacttt ttccgaata tttgtgcca 
      accgttgct agatctgat gtgcataca ggacgactc 
      tgaagaggt gaagaaggc ggtcaaaac ggtggcctt 
      actcgccct aggtataat aacatacat caacgtttc 
      aagcgtgaa actctacgt caaagtgca gaacatttt 
      cggcggatt taaagtatt cgcctgttt ggtggtgat 
      ttttggcgg cctttacga aatcatcaa aggcaacac 
      tgacaattt aaaccgggg cttcgaagg cacaaagta 
      cacaccgaa aagcatcgc tttggcctt ttatagcgg 
      actttgggc ttatgacgg atggtctgc ggtcactgt 
      aatcactga agagatcaa acgacctga agtaaacat 
      cccgaggag cataatgat aagtgttcc catagtcac 
      cggtttgta tgtctttat gaacatggc atacatgac 
      cgcattatc gatacctga gatgataaa ttcgcctgc 
      tgttgctgt aactttcgg tgaaagggt tttaggccc 
      tttggcgtt tttgatacc actaggagt ggcgttagc 
      tacgtttgg atgtgccct aagtataca atttggggt 
      cactagggt gtgttatgt ggcaggaca ggacggcct 
      aatgatcaa aagtctttc cttcgttca tcatgaacg 
      tctaactcc gtcattcgc tgtcgtttc tcaggggat 
      tttatcgct cctttttat cgtagctgg ggatattgt 
      tgaactgat cgaatttgt tagtttcct catttggat 
      attctacgg gctggcttt tgtctcgtt acttgtctt 
      aaggaggac tatgaaaga tgctcctcg accctacaa 
      agtgcctac atggatttg tgttttcgt tttattggt 
      agctattta tttgagtgt tacgcccat tattacgga 
      tccaactcc gaaatattt gctcgcttt ggcgtttat 
      tttggtggg ggttttggt ttattattg gtttgttta 
      caagaagcg aacaccgga attgttgat gagaaagtt 
      tacacgttt tattcagat tttattcgc ggctgtgcc 
      acccgataa tccaaatgc tacataaga aattattta 
      ttgagatgt tatgtaatt tataattgc agttgtaat 
      aaaatgaaa tataaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY