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Gene information
General information
TC032034
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000785 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5557717..5559250
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032034
    iBeetle screen iB_10679

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032034 RA
      MYPIITFIE DLHEFLTGD RLVKGNIIR KHSKKLDRL 
GDLSFPLSF KNWSQNITI SDTNLRTIF DYLSSGQDL 
QSQLDSLVK QSEKWALTV RECKLINNS DVHLYLKRS 
DTIDLCIKK VLNERENYG LLHKCFTKK ITVSLDHTF 
ECDFAKMTL TELRALITH DVTSNVLAC LSANNSDSD 
CDIKITINS NQKQDFTLV CGSVLNENG LKDTKTTAF 
ELYRKRALD LQLMAQHKY GVQVKPSWG DYFDKLGKA 
SVVIEMLQN RLHKTIKIT LNNLQATNK GPSFIFYNC 
ARLSSLLKQ FDERVANKL YPELPLLEK IDFTLLNQP 
EEWEIVYVY ILQFPTVVI STVCDIKSG LINLHNLII 
FLSNLSSIF SVYYRRVKI LISAKEHLL PTLFARIYL 
LKAVQQVFH TALKLLNIE PVKEM
    CDS sequence
    • >TC032034 RA
      atgtacccc attatcaca tttatcgaa gacttgcac 
gaattttta acaggggac aggttggtg aagggtaac 
ataattcgt aaacattca aaaaaactt gaccgtttg 
ggagatttg agcttccct ttgagtttt aaaaattgg 
tctcaaaac attacaatt agtgacacg aatttacgc 
acaattttt gactattta tcgtcggga caagaccta 
caatctcaa ctagattca ctggtaaag caaagcgaa 
aagtgggct ttaacagta agggaatgc aaattaata 
aataacagt gatgttcat ttatacctg aaaaggtcg 
gacacgatt gatttatgc ataaaaaag gtcttgaac 
gagagagaa aattacggc ttgctgcac aaatgtttc 
actaaaaaa attactgtg tctcttgat cacaccttt 
gaatgcgat tttgcaaaa atgacttta accgaactg 
cgagcttta attactcac gatgttaca tcaaacgtt 
cttgcatgt ttaagtgca aataattct gacagtgat 
tgcgatatt aagatcaca ataaactca aatcaaaag 
caagacttc actttagtt tgtggttca gttttgaat 
gaaaatggt ctcaaagac acaaaaaca acagctttt 
gaactttac agaaagcga gctttagat ctgcaacta 
atggcccag cataaatat ggtgttcag gtcaaacca 
tcatggggt gattatttc gataagttg ggaaaagcg 
tcagttgtg attgaaatg ttgcaaaat aggttgcat 
aaaacgata aaaattacg cttaacaat ttgcaagca 
acaaataaa ggcccatcg tttattttt tacaattgt 
gcacgactg tcgtcactg ttgaagcag tttgatgag 
cgagtcgca aataaatta tatcctgag ttacctctc 
ttggaaaaa atcgatttc actttgctt aaccaacct 
gaagaatgg gaaatcgtc tacgtttat attttacaa 
tttccaact gttgttatt agcacagtg tgtgatatt 
aagtcaggt ttaataaat cttcataat ttgataatt 
tttttgtca aacttgtcg tccatcttc agcgtgtat 
tacaggagg gtaaaaata ctcattagt gccaaggaa 
caccttctg cccacgtta tttgcgaga atttattta 
ctaaaagca gtacaacaa gtatttcac actgcttta 
aaactgtta aatatagaa ccagttaaa gaaatgtaa
    mRNA sequence
    • >TC032034 RA
      tataaaata aaaaattgt gtatttttg ccccaaaat 
gtaccccat tatcacatt tatcgaaga cttgcacga 
atttttaac aggggacag gttggtgaa gggtaacat 
aattcgtaa acattcaaa aaaacttga ccgtttggg 
agatttgag cttcccttt gagttttaa aaattggtc 
tcaaaacat tacaattag tgacacgaa tttacgcac 
aatttttga ctatttatc gtcgggaca agacctaca 
atctcaact agattcact ggtaaagca aagcgaaaa 
gtgggcttt aacagtaag ggaatgcaa attaataaa 
taacagtga tgttcattt atacctgaa aaggtcgga 
cacgattga tttatgcat aaaaaaggt cttgaacga 
gagagaaaa ttacggctt gctgcacaa atgtttcac 
taaaaaaat tactgtgtc tcttgatca cacctttga 
atgcgattt tgcaaaaat gactttaac cgaactgcg 
agctttaat tactcacga tgttacatc aaacgttct 
tgcatgttt aagtgcaaa taattctga cagtgattg 
cgatattaa gatcacaat aaactcaaa tcaaaagca 
agacttcac tttagtttg tggttcagt tttgaatga 
aaatggtct caaagacac aaaaacaac agcttttga 
actttacag aaagcgagc tttagatct gcaactaat 
ggcccagca taaatatgg tgttcaggt caaaccatc 
atggggtga ttatttcga taagttggg aaaagcgtc 
agttgtgat tgaaatgtt gcaaaatag gttgcataa 
aacgataaa aattacgct taacaattt gcaagcaac 
aaataaagg cccatcgtt tatttttta caattgtgc 
acgactgtc gtcactgtt gaagcagtt tgatgagcg 
agtcgcaaa taaattata tcctgagtt acctctctt 
ggaaaaaat cgatttcac tttgcttaa ccaacctga 
agaatggga aatcgtcta cgtttatat tttacaatt 
tccaactgt tgttattag cacagtgtg tgatattaa 
gtcaggttt aataaatct tcataattt gataatttt 
tttgtcaaa cttgtcgtc catcttcag cgtgtatta 
caggagggt aaaaatact cattagtgc caaggaaca 
ccttctgcc cacgttatt tgcgagaat ttatttact 
aaaagcagt acaacaagt atttcacac tgctttaaa 
actgttaaa tatagaacc agttaaaga aatgtaata 
aaaccaatt gtatttttc tacttgaat aaattattg 
tttgacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY