Status
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Gene information
General information
TC033277
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012994
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15428483..15431446
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033277
    iBeetle screen iB_10677

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033277 RA
      MGSDSNKEY SVNWKNHMD HMRKAFDNL LTSNELTDV 
      TLCCEGRRI GAHKMLLSA CSTYFRDTF KDVPCQHPV 
      IILYGVEYS VLSDILHFI YNGEVSVDT SKLDSFLKT 
      AQLLKISGL TDNSTENMA SENSEQDTQ AASRREQTE 
      GAFSEQVST ASEDTKKGA KRRVQESEG APTQTKVGR 
      QESDTLFMS DVKSEPSEF VNYEDPLED PLLQADPSN 
      ENREFFVTS QGGVEDEIK NRSRLLKVE KNGRRLSLN 
      GYKYTANKR KYSKIYWRC HKYKCTKCN GRLTTDLEF 
      NLINVNEEH VHPPYLL
    • >TC033277 RB
      MGSDSNKEY SVNWKNHMD HMRKAFDNL LTSNELTDV 
      TLCCEGRRI GAHKMLLSA CSTYFRDTF KDVPCQHPV 
      IILYGVEYS VLSDILHFI YNGEVSVDT SKLDSFLKT 
      AQLLKISGL TDNSTENMA SENSEQDTQ AASRREQTE 
      GAFSEQVST ASEDTKKGA KRRVQESEG APTQTKVGR 
      QESDTLFMS DVKSEPSEF VNYEDPLED PLLQADPSN 
      ENREFFVTS QGGVEDEIK MKASKSTSW PINTVKCNI 
      CNKVFSHPY SLQQHKPVH AGNTRCPIC KAVLSRKYH 
      LKLHMKARH NLN
    • >TC033277 RC
      MGSDSNKEY SVNWKNHMD HMRKAFDNL LTSNELTDV 
      TLCCEGRRI GAHKMLLSA CSTYFRDTF KDVPCQHPV 
      IILYGVEYS VLSDILHFI YNGEVSVDT SKLDSFLKT 
      AQLLKISGL TDNSTENMA SENSEQDTQ AASRREQTE 
      GAFSEQVST ASEDTKKGA KRRVQESEG APTQTKVGR 
      QESDTLFMS DVKSEPSEF VNYEDPLED PLLQADPSN 
      ENREFFVTS QGGVEDEIK IERTSLETT TLVRDTDIK 
      VNKSNFSEE SLRCPICSK LFSHPYSLH HHKAVHSGN 
      TECSICHVV LSRKYNLKM HMKARHNL
    CDS sequence
    • >TC033277 RA
      atggggtcc gattccaat aaggaatat tcagtgaat 
      tggaaaaac cacatggac catatgcga aaagcgttc 
      gataattta ctaacttcg aacgaacta accgatgtt 
      actttgtgt tgtgagggt cgtaggatt ggggcccat 
      aaaatgctc ctctcggca tgtagtacc tactttcgg 
      gacactttt aaagacgtg ccttgccag caccctgtc 
      attattttg tacggggtg gaatatagt gttttgtca 
      gatattctt cattttatt tacaatggg gaagttagt 
      gtagatacc tcaaaactg gactcgttt ttaaaaaca 
      gcacagttg ttaaaaatt agtggactc actgataac 
      tcaactgaa aatatggca agtgaaaat agtgagcag 
      gacacacaa gcggccagt agaagggaa cagacagag 
      ggggctttc agcgaacaa gtatcaaca gcaagtgag 
      gacaccaaa aaaggggcg aagaggaga gtacaagaa 
      tcagaagga gcaccaacg cagactaaa gtgggtagg 
      caagaaagt gatacgctt tttatgtcg gatgtaaag 
      tcggaacct agtgagttt gtcaattat gaggatcct 
      ttagaagac ccactttta caagctgat ccatccaat 
      gaaaatagg gaatttttt gtgacaagt caggggggc 
      gtcgaggac gagattaaa aatagaagt cgtcttctg 
      aaagttgaa aaaaatggt cgaagattg tctttaaac 
      ggttataaa tacacagct aacaaacga aaatatagc 
      aaaatttat tggagatgt cataaatat aaatgtact 
      aaatgcaat ggacgactt actacggat ttggaattc 
      aatctaata aatgtcaat gaggagcat gtgcatccg 
      ccatactta ttgtaa
    • >TC033277 RB
      atggggtcc gattccaat aaggaatat tcagtgaat 
      tggaaaaac cacatggac catatgcga aaagcgttc 
      gataattta ctaacttcg aacgaacta accgatgtt 
      actttgtgt tgtgagggt cgtaggatt ggggcccat 
      aaaatgctc ctctcggca tgtagtacc tactttcgg 
      gacactttt aaagacgtg ccttgccag caccctgtc 
      attattttg tacggggtg gaatatagt gttttgtca 
      gatattctt cattttatt tacaatggg gaagttagt 
      gtagatacc tcaaaactg gactcgttt ttaaaaaca 
      gcacagttg ttaaaaatt agtggactc actgataac 
      tcaactgaa aatatggca agtgaaaat agtgagcag 
      gacacacaa gcggccagt agaagggaa cagacagag 
      ggggctttc agcgaacaa gtatcaaca gcaagtgag 
      gacaccaaa aaaggggcg aagaggaga gtacaagaa 
      tcagaagga gcaccaacg cagactaaa gtgggtagg 
      caagaaagt gatacgctt tttatgtcg gatgtaaag 
      tcggaacct agtgagttt gtcaattat gaggatcct 
      ttagaagac ccactttta caagctgat ccatccaat 
      gaaaatagg gaatttttt gtgacaagt caggggggc 
      gtcgaggac gagattaaa atgaaggcc agcaaaagc 
      acatcatgg ccgattaat accgtcaag tgtaacatc 
      tgtaataaa gttttttcg catccgtat agtctacaa 
      caacataaa ccggtccat gctggaaat acgcgatgt 
      ccgatttgt aaagccgtt ctttccagg aagtatcat 
      ctcaagttg cacatgaag gcgaggcat aatctgaac 
      tga
    • >TC033277 RC
      atggggtcc gattccaat aaggaatat tcagtgaat 
      tggaaaaac cacatggac catatgcga aaagcgttc 
      gataattta ctaacttcg aacgaacta accgatgtt 
      actttgtgt tgtgagggt cgtaggatt ggggcccat 
      aaaatgctc ctctcggca tgtagtacc tactttcgg 
      gacactttt aaagacgtg ccttgccag caccctgtc 
      attattttg tacggggtg gaatatagt gttttgtca 
      gatattctt cattttatt tacaatggg gaagttagt 
      gtagatacc tcaaaactg gactcgttt ttaaaaaca 
      gcacagttg ttaaaaatt agtggactc actgataac 
      tcaactgaa aatatggca agtgaaaat agtgagcag 
      gacacacaa gcggccagt agaagggaa cagacagag 
      ggggctttc agcgaacaa gtatcaaca gcaagtgag 
      gacaccaaa aaaggggcg aagaggaga gtacaagaa 
      tcagaagga gcaccaacg cagactaaa gtgggtagg 
      caagaaagt gatacgctt tttatgtcg gatgtaaag 
      tcggaacct agtgagttt gtcaattat gaggatcct 
      ttagaagac ccactttta caagctgat ccatccaat 
      gaaaatagg gaatttttt gtgacaagt caggggggc 
      gtcgaggac gagattaaa attgagagg acatctctc 
      gagacaacg acactggtg cgggatacc gacatcaaa 
      gtcaataag agtaatttt tccgaggag tcgttgagg 
      tgtcccatt tgcagcaag ttattttct catccgtac 
      agcttacac catcacaaa gccgtgcat tcgggcaac 
      actgaatgt tcgatatgt catgttgta ctctcgcga 
      aaatacaac ttgaaaatg cacatgaaa gcgcgtcac 
      aacttgtaa
    mRNA sequence
    • >TC033277 RA
      acttcgcca tttgttttt tgacatttc tcaaataaa 
      taattcgtt aataaataa atgggagcg tggaaaatc 
      gcctgttac ataacactt gacttattc tgagccctt 
      ttttcgact tgtgggtga aaaagtgat tcagtgcag 
      ttattgacc ctttttgca acagtgacc gtggccatc 
      aagttaact gtaaccgaa tgtttacgt agtcttcgt 
      tcatttgtt agtgacagt agtgataaa aaatggggt 
      ccgattcca ataaggaat attcagtga attggaaaa 
      accacatgg accatatgc gaaaagcgt tcgataatt 
      tactaactt cgaacgaac taaccgatg ttactttgt 
      gttgtgagg gtcgtagga ttggggccc ataaaatgc 
      tcctctcgg catgtagta cctactttc gggacactt 
      ttaaagacg tgccttgcc agcaccctg tcattattt 
      tgtacgggg tggaatata gtgttttgt cagatattc 
      ttcatttta tttacaatg gggaagtta gtgtagata 
      cctcaaaac tggactcgt ttttaaaaa cagcacagt 
      tgttaaaaa ttagtggac tcactgata actcaactg 
      aaaatatgg caagtgaaa atagtgagc aggacacac 
      aagcggcca gtagaaggg aacagacag agggggctt 
      tcagcgaac aagtatcaa cagcaagtg aggacacca 
      aaaaagggg cgaagagga gagtacaag aatcagaag 
      gagcaccaa cgcagacta aagtgggta ggcaagaaa 
      gtgatacgc tttttatgt cggatgtaa agtcggaac 
      ctagtgagt ttgtcaatt atgaggatc ctttagaag 
      acccacttt tacaagctg atccatcca atgaaaata 
      gggaatttt ttgtgacaa gtcaggggg gcgtcgagg 
      acgagatta aaaatagaa gtcgtcttc tgaaagttg 
      aaaaaaatg gtcgaagat tgtctttaa acggttata 
      aatacacag ctaacaaac gaaaatata gcaaaattt 
      attggagat gtcataaat ataaatgta ctaaatgca 
      atggacgac ttactacgg atttggaat tcaatctaa 
      taaatgtca atgaggagc atgtgcatc cgccatact 
      tattgtaat tttaggtac agtagaacc ccttcaaac 
      cgaatctct tgtattggg aacttttta cacattgta 
      tattttatt atctatttt tgaataaaa atacatatt 
      ttagttatt gttcatttc agtataacc ccttgcaac 
      aattagttc ggattgtag aggttttac tgtataaat 
      tgcaaagca acacaacac ttggatttg taacgtcca 
      tctgcaagc tttctgctt taattaacc caaagtttt 
      gtactacgt gattatgat ttttgtgat tatgcttgt 
      tgttacatt gtaacgtga gacttgtaa aacgtaacc 
      gaaaattga aagcgttcc ttttacaag tcttgcaga 
      tgtgagtta ccatccctg atgttgtat tgttttcca 
      gttgagagg acatctctc gagacaacg acactggtg 
      cgggatacc gacatcaaa gtcaataag agtaatttt 
      tccgaggag tcgttgagg tgtcccatt tgcagcaag 
      ttattttct catccgtac agcttacac catcacaaa 
      gccgtgcat tcgggcaac actgaatgt tcgatatgt 
      catgttgta ctctcgcga aaatacaac ttgaaaatg 
      cacatgaaa gcgcgtcac aacttgtaa aaaaatgtg 
      tggtgtttt tgcagtgaa ggccagcaa aagcacatc 
      atggccgat taataccgt caagtgtaa catctgtaa 
      taaagtttt ttcgcatcc gtatagtct acaacaaca 
      taaaccggt ccatgctgg aaatacgcg atgtccgat 
      ttgtaaagc cgttctttc caggaagta tcatctcaa 
      gttgcacat gaaggcgag gcataatct gaactgacg 
      tactgttac tgtttatta aaatcaccc ttaagtttg 
      tgtgaagtg tgatggatt ttgcacttc aaaaattgt 
      tgtttttgt ttaaaccac aaaggaata cttgtgggc 
      ttagattag attttttac atttattat aaagaaata 
      catattttt tttatagtg taacaaatt tctgttcag 
      ctttattat aatattgtt tgtggatga tgcatttat 
      gtttatgtt tatgtacag ttaccataa ttcttttca 
      ctttcagcg taagtacaa aactgttaa tcgtgttta 
      tttttaaaa tgttttttg tcgaatagg ttcttaaaa 
      ataagtctg ttgatctaa atctttgta tgtaatctt 
      tttaagaat gtcatcaga tgacatatc taataaaca 
      tttaatttg aat
    • >TC033277 RB
      acttcgcca tttgttttt tgacatttc tcaaataaa 
      taattcgtt aataaataa atgggagcg tggaaaatc 
      gcctgttac ataacactt gacttattc tgagccctt 
      ttttcgact tgtgggtga aaaagtgat tcagtgcag 
      ttattgacc ctttttgca acagtgacc gtggccatc 
      aagttaact gtaaccgaa tgtttacgt agtcttcgt 
      tcatttgtt agtgacagt agtgataaa aaatggggt 
      ccgattcca ataaggaat attcagtga attggaaaa 
      accacatgg accatatgc gaaaagcgt tcgataatt 
      tactaactt cgaacgaac taaccgatg ttactttgt 
      gttgtgagg gtcgtagga ttggggccc ataaaatgc 
      tcctctcgg catgtagta cctactttc gggacactt 
      ttaaagacg tgccttgcc agcaccctg tcattattt 
      tgtacgggg tggaatata gtgttttgt cagatattc 
      ttcatttta tttacaatg gggaagtta gtgtagata 
      cctcaaaac tggactcgt ttttaaaaa cagcacagt 
      tgttaaaaa ttagtggac tcactgata actcaactg 
      aaaatatgg caagtgaaa atagtgagc aggacacac 
      aagcggcca gtagaaggg aacagacag agggggctt 
      tcagcgaac aagtatcaa cagcaagtg aggacacca 
      aaaaagggg cgaagagga gagtacaag aatcagaag 
      gagcaccaa cgcagacta aagtgggta ggcaagaaa 
      gtgatacgc tttttatgt cggatgtaa agtcggaac 
      ctagtgagt ttgtcaatt atgaggatc ctttagaag 
      acccacttt tacaagctg atccatcca atgaaaata 
      gggaatttt ttgtgacaa gtcaggggg gcgtcgagg 
      acgagatta aaatgaagg ccagcaaaa gcacatcat 
      ggccgatta ataccgtca agtgtaaca tctgtaata 
      aagtttttt cgcatccgt atagtctac aacaacata 
      aaccggtcc atgctggaa atacgcgat gtccgattt 
      gtaaagccg ttctttcca ggaagtatc atctcaagt 
      tgcacatga aggcgaggc ataatctga actgacgta 
      ctgttactg tttattaaa atcaccctt aagtttgtg 
      tgaagtgtg atggatttt gcacttcaa aaattgttg 
      tttttgttt aaaccacaa aggaatact tgtgggctt 
      agattagat tttttacat ttattataa agaaataca 
      tattttttt tatagtgta acaaatttc tgttcagct 
      ttattataa tattgtttg tggatgatg catttatgt 
      ttatgttta tgtacagtt accataatt cttttcact 
      ttcagcgta agtacaaaa ctgttaatc gtgtttatt 
      tttaaaatg ttttttgtc gaataggtt cttaaaaat 
      aagtctgtt gatctaaat ctttgtatg taatctttt 
      taagaatgt catcagatg acatatcta ataaacatt 
      taatttgaa t
    • >TC033277 RC
      acttcgcca tttgttttt tgacatttc tcaaataaa 
      taattcgtt aataaataa atgggagcg tggaaaatc 
      gcctgttac ataacactt gacttattc tgagccctt 
      ttttcgact tgtgggtga aaaagtgat tcagtgcag 
      ttattgacc ctttttgca acagtgacc gtggccatc 
      aagttaact gtaaccgaa tgtttacgt agtcttcgt 
      tcatttgtt agtgacagt agtgataaa aaatggggt 
      ccgattcca ataaggaat attcagtga attggaaaa 
      accacatgg accatatgc gaaaagcgt tcgataatt 
      tactaactt cgaacgaac taaccgatg ttactttgt 
      gttgtgagg gtcgtagga ttggggccc ataaaatgc 
      tcctctcgg catgtagta cctactttc gggacactt 
      ttaaagacg tgccttgcc agcaccctg tcattattt 
      tgtacgggg tggaatata gtgttttgt cagatattc 
      ttcatttta tttacaatg gggaagtta gtgtagata 
      cctcaaaac tggactcgt ttttaaaaa cagcacagt 
      tgttaaaaa ttagtggac tcactgata actcaactg 
      aaaatatgg caagtgaaa atagtgagc aggacacac 
      aagcggcca gtagaaggg aacagacag agggggctt 
      tcagcgaac aagtatcaa cagcaagtg aggacacca 
      aaaaagggg cgaagagga gagtacaag aatcagaag 
      gagcaccaa cgcagacta aagtgggta ggcaagaaa 
      gtgatacgc tttttatgt cggatgtaa agtcggaac 
      ctagtgagt ttgtcaatt atgaggatc ctttagaag 
      acccacttt tacaagctg atccatcca atgaaaata 
      gggaatttt ttgtgacaa gtcaggggg gcgtcgagg 
      acgagatta aaattgaga ggacatctc tcgagacaa 
      cgacactgg tgcgggata ccgacatca aagtcaata 
      agagtaatt tttccgagg agtcgttga ggtgtccca 
      tttgcagca agttatttt ctcatccgt acagcttac 
      accatcaca aagccgtgc attcgggca acactgaat 
      gttcgatat gtcatgttg tactctcgc gaaaataca 
      acttgaaaa tgcacatga aagcgcgtc acaacttgt 
      aaaaaaatg tgtggtgtt tttgcagtg aaggccagc 
      aaaagcaca tcatggccg attaatacc gtcaagtgt 
      aacatctgt aataaagtt ttttcgcat ccgtatagt 
      ctacaacaa cataaaccg gtccatgct ggaaatacg 
      cgatgtccg atttgtaaa gccgttctt tccaggaag 
      tatcatctc aagttgcac atgaaggcg aggcataat 
      ctgaactga cgtactgtt actgtttat taaaatcac 
      ccttaagtt tgtgtgaag tgtgatgga ttttgcact 
      tcaaaaatt gttgttttt gtttaaacc acaaaggaa 
      tacttgtgg gcttagatt agatttttt acatttatt 
      ataaagaaa tacatattt tttttatag tgtaacaaa 
      tttctgttc agctttatt ataatattg tttgtggat 
      gatgcattt atgtttatg tttatgtac agttaccat 
      aattctttt cactttcag cgtaagtac aaaactgtt 
      aatcgtgtt tatttttaa aatgttttt tgtcgaata 
      ggttcttaa aaataagtc tgttgatct aaatctttg 
      tatgtaatc tttttaaga atgtcatca gatgacata 
      tctaataaa catttaatt tgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY