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Gene information
General information
TC013117 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13510574..13512021
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013117
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10676

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013117 RA
      MALSPRESG KLVASLSKN VKIKDEGVK KLGDLLVSE 
      IESGKLSSE KFSQVKLHP KSDDPKALD WLFVVDTLN 
      FCFWHLETE AGWTVEGYT GYFALCAAI NRAVKEKVD 
      ILNSKYYSE VSEKDLAKI FRSDNDVPI PLLQERVKC 
      LHEVGNVLL ETFDGSFEN CVKKADKSA VALLDLIVN 
      NFKCFKDEA TYKDKKIAI YKRAQILIG DIWACFKNE 
      GLGHFKDID EITMFADYR IPQSLLYFG VFEYSEDLK 
      KKLQDNKIL ENGEEDEVE IRACSIHAV ELLKEHANK 
      KLTDTKINA ILIDHFLWD FRREHNKEI VEKKLPFHK 
      TFSIYY
    CDS sequence
    • >TC013117 RA
      atggcttta tcacctcgt gagtcaggc aaactcgtt 
      gcttcgctt agcaaaaac gtgaaaatc aaagacgaa 
      ggtgtgaaa aaactgggg gatttgttg gttagcgag 
      atcgaaagc gggaaattg tccagtgag aagttcagt 
      caagtgaaa ctgcaccca aaatccgac gatcccaaa 
      gcccttgac tggttattt gtcgtggac actctgaat 
      ttttgcttc tggcactta gaaaccgaa gctggctgg 
      actgtggag ggctacact ggttatttt gccctttgt 
      gcggctatt aatcgagcc gtgaaggaa aaagtggat 
      attttgaac tcgaaatac tactctgaa gtgtccgaa 
      aaagacctt gcgaaaata ttcagaagc gacaacgat 
      gtcccaatt ccactcctc caggaacgc gtcaaatgc 
      ttgcacgaa gttgggaac gtccttctt gaaactttc 
      gacggttcg tttgaaaat tgcgtgaaa aaggctgac 
      aagagcgct gtggccctt ctggaccta atcgtcaac 
      aatttcaag tgcttcaag gacgaagcg acttacaaa 
      gacaagaaa attgcgatt tacaagcgg gcgcagatt 
      ttaattggc gacatttgg gcctgtttc aagaacgaa 
      ggacttggg cactttaaa gatatcgat gaaataaca 
      atgtttgcg gattataga atcccacaa tctctccta 
      tatttcggg gtgtttgag tacagcgaa gatttgaag 
      aagaaactg caagacaac aaaattctc gaaaacggg 
      gaagaggac gaagtggag atcagagct tgctccatc 
      catgcggtc gagctgcta aaagaacat gccaataaa 
      aaactaact gatacgaaa atcaatgcc attttgatc 
      gatcacttc ttgtgggac ttcagacgc gaacataac 
      aaggaaatc gtggagaag aagttgccg ttccacaaa 
      acgttctcc atttattac taa
    mRNA sequence
    • >TC013117 RA
      ggtagtaac gcacttcag caccagagg cgctgtatt 
      ctcgtgttg ccagtgatt tactaggga attatcact 
      tcttaacct ttactgttc tgtgatttt acaagctcc 
      gcgttttca caacatatc aagagaaaa tggctttat 
      cacctcgtg agtcaggca aactcgttg cttcgctta 
      gcaaaaacg tgaaaatca aagacgaag gtgtgaaaa 
      aactggggg atttgttgg ttagcgaga tcgaaagcg 
      ggaaattgt ccagtgaga agttcagtc aagtgaaac 
      tgcacccaa aatccgacg atcccaaag cccttgact 
      ggttatttg tcgtggaca ctctgaatt tttgcttct 
      ggcacttag aaaccgaag ctggctgga ctgtggagg 
      gctacactg gttattttg ccctttgtg cggctatta 
      atcgagccg tgaaggaaa aagtggata ttttgaact 
      cgaaatact actctgaag tgtccgaaa aagaccttg 
      cgaaaatat tcagaagcg acaacgatg tcccaattc 
      cactcctcc aggaacgcg tcaaatgct tgcacgaag 
      ttgggaacg tccttcttg aaactttcg acggttcgt 
      ttgaaaatt gcgtgaaaa aggctgaca agagcgctg 
      tggcccttc tggacctaa tcgtcaaca atttcaagt 
      gcttcaagg acgaagcga cttacaaag acaagaaaa 
      ttgcgattt acaagcggg cgcagattt taattggcg 
      acatttggg cctgtttca agaacgaag gacttgggc 
      actttaaag atatcgatg aaataacaa tgtttgcgg 
      attatagaa tcccacaat ctctcctat atttcgggg 
      tgtttgagt acagcgaag atttgaaga agaaactgc 
      aagacaaca aaattctcg aaaacgggg aagaggacg 
      aagtggaga tcagagctt gctccatcc atgcggtcg 
      agctgctaa aagaacatg ccaataaaa aactaactg 
      atacgaaaa tcaatgcca ttttgatcg atcacttct 
      tgtgggact tcagacgcg aacataaca aggaaatcg 
      tggagaaga agttgccgt tccacaaaa cgttctcca 
      tttattact aaatcgaac gacaatatc aagttaaaa 
      ttggagttg accgaatca atgtgctaa tttattagt 
      aattaatcg aaaatttca gggagcggt tggccatga 
      aactttgat atggcattt tggtggtag tcatggtta 
      caattgtta caaaccgaa ccgtacttt ttcattgcc 
      aactatgct gtcatgttt tatttcata tcagtaatt 
      tttaagatt aatcgtgtc aatgttttt aaagttaaa 
      gtattagat tatgattga aagaatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY