Status
Please wait ...
Gene information
General information
TC006564 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11435389..11437749
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006564
    iBeetle screen iB_10675

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006564 RA
      MASGNNFPF VQEFVADPL DGITLLLDL LRAIQLSQS 
      SGTTTTGKI PPSLQRRAL LDELSCLQC LLLCCQRYN 
      EAVRKLTSS SAGLFTLAI CIMSNVTKS RILALQLLT 
      KACNNGHSA VSEALATLR LRFGEPVRF RFLVGMLMS 
      AGGQGELLV AGMKFLNTF LDTSGTMQK RIYIQAELE 
      QAGFDIATI KKQISINSA STEQLFEEL DHWEKKFLD 
      VESLSIRLE STERENDSL RDKVLLLER RVQILLEEK 
      GILISLEQC LKERCSELQ EEVHSLKSE KSQKNSSCG 
      SSKKKEDSP HEDEGISSS ERSLTPDEV LQRESSVYE 
      LYNVQNPIM KKPSKIEEE DETTIDEVI EELRNIIND 
      AETETYRTE EKKKLERKK IEEAQVASK IQMTVAIDD 
      FSKEIEIVP SNLHPQPPR RTKSLVHLF IPAEDYDYG 
      NKELFFENE TPYTSEEGS DSLLSASKY QLPRVEKGR 
      KPVHRSESF RQAKQPQNP VSKQNSFDG GYFSVGVVA 
      EDSKKIKSK SLDRIDDGL ETLVDIVVT AEHEVPKPE 
      SLLNHQKKF SDEKMKMFL PKRDNDVLY YFPRIQERK 
      NNTFLARGH NAGLYSGQV TRQNSVKKP EFVSGNNRG 
      KVTDLPSGL Y
    CDS sequence
    • >TC006564 RA
      atggcttct ggcaacaat tttcccttc gtccaagaa 
      ttcgtcgcc gatccactc gacggaatc actctcctc 
      cttgacttg ctccgagca attcaattg agtcagtcg 
      agtggcacc accaccacg ggcaaaatc cctccatcc 
      ctccagcgg cgcgccctc ctcgacgag ctctcctgc 
      ctccaatgc ctcctgctc tgctgtcaa agatacaac 
      gaagcggtc cgaaaactc acatcttcc tcagccggg 
      cttttcact ctagccatt tgcattatg agcaatgtg 
      acgaaatcg cgaattctg gcccttcag ttactgacc 
      aaggcctgt aataacggc cacagcgcg gtctccgaa 
      gccctagcc acgctcagg cttaggttt ggggagccg 
      gtcaggttt aggtttctg gtgggcatg ctcatgtcg 
      gcagggggg caaggcgag ctgctagtg gccgggatg 
      aaattcctg aatacgttc ctggacacg tccgggacc 
      atgcaaaag aggatttat atccaagcc gagttggaa 
      caggcaggt tttgatatc gcaacgatc aaaaaacaa 
      attagtatt aattcagcc tcgactgag caacttttc 
      gaggagttg gatcattgg gagaagaag tttctcgat 
      gttgaaagt ctctcaatc aggcttgag agcacggaa 
      cgtgaaaat gactcgttg agggataaa gtgttgttg 
      ttggaacga cgagttcag attcttttg gaggaaaaa 
      gggattttg atttcgctc gagcagtgt ctgaaagaa 
      cgctgcagt gaattgcag gaggaagtc cattcgttg 
      aagtccgaa aagtcgcaa aagaactct tcgtgtgga 
      agttcgaag aagaaagaa gactctccc catgaagac 
      gagggcatt tcttcatcc gagagaagc ctcacgcct 
      gacgaagtc ctccagcgc gaatcctcc gtctacgag 
      ctgtacaac gtccaaaac cccatcatg aagaaaccg 
      tcaaaaatc gaagaagaa gacgaaaca accatcgac 
      gaagtgatc gaagagtta cggaacata ataaacgac 
      gcggaaacc gagacctac cgaacggaa gaaaagaag 
      aaactcgaa cgcaaaaaa attgaagaa gcacaagtc 
      gccagcaaa atccaaatg acggtggcc atcgacgac 
      ttcagcaaa gaaatagaa atagtccct tcgaacctc 
      cacccccag ccccctcga cgcaccaaa agcctcgtc 
      cacttattt atcccagct gaagattac gattacggt 
      aacaaggag ttatttttc gaaaacgaa acaccgtac 
      actagtgaa gaaggctcc gattcgctc ctaagtgcc 
      tccaagtac caactacca cgggtggaa aaggggcgc 
      aagcccgtc caccggtcg gagtccttc agacaagcc 
      aagcagccc caaaacccc gtctcgaaa cagaacagc 
      ttcgacggg ggttatttc agcgtgggg gttgtcgcc 
      gaagactcg aaaaaaatc aagtcgaag agtttggac 
      cgaatcgac gacggttta gagactttg gttgatatt 
      gtggtcaca gcagaacac gaagtcccc aagcctgag 
      tcgctcctc aaccaccag aagaagttc agtgatgag 
      aaaatgaag atgtttttg cccaagcgc gacaatgac 
      gtgctttat tacttcccg aggatacaa gagcggaaa 
      aataacacg ttcctggcg cggggccac aacgccggg 
      ttgtactcg gggcaagtg acaagacaa aactcggtg 
      aagaaaccg gagtttgtc tcggggaat aaccggggg 
      aaagtgacg gatttaccg tcgggactt tactga
    mRNA sequence
    • >TC006564 RA
      aaagaaaaa gccatggct tctggcaac aattttccc 
      ttcgtccaa gaattcgtc gccgatcca ctcgacgga 
      atcactctc ctccttgac ttgctccga gcaattcaa 
      ttgagtcag tcgagtggc accaccacc acgggcaaa 
      atccctcca tccctccag cggcgcgcc ctcctcgac 
      gagctctcc tgcctccaa tgcctcctg ctctgctgt 
      caaagatac aacgaagcg gtccgaaaa ctcacatct 
      tcctcagcc gggcttttc actctagcc atttgcatt 
      atgagcaat gtgacgaaa tcgcgaatt ctggccctt 
      cagttactg accaaggcc tgtaataac ggccacagc 
      gcggtctcc gaagcccta gccacgctc aggcttagg 
      tttggggag ccggtcagg tttaggttt ctggtgggc 
      atgctcatg tcggcaggg gggcaaggc gagctgcta 
      gtggccggg atgaaattc ctgaatacg ttcctggac 
      acgtccggg accatgcaa aagaggatt tatatccaa 
      gccgagttg gaacaggca ggttttgat atcgcaacg 
      atcaaaaaa caaattagt attaattca gcctcgact 
      gagcaactt ttcgaggag ttggatcat tgggagaag 
      aagtttctc gatgttgaa agtctctca atcaggctt 
      gagagcacg gaacgtgaa aatgactcg ttgagggat 
      aaagtgttg ttgttggaa cgacgagtt cagattctt 
      ttggaggaa aaagggatt ttgatttcg ctcgagcag 
      tgtctgaaa gaacgctgc agtgaattg caggaggaa 
      gtccattcg ttgaagtcc gaaaagtcg caaaagaac 
      tcttcgtgt ggaagttcg aagaagaaa gaagactct 
      ccccatgaa gacgagggc atttcttca tccgagaga 
      agcctcacg cctgacgaa gtcctccag cgcgaatcc 
      tccgtctac gagctgtac aacgtccaa aaccccatc 
      atgaagaaa ccgtcaaaa atcgaagaa gaagacgaa 
      acaaccatc gacgaagtg atcgaagag ttacggaac 
      ataataaac gacgcggaa accgagacc taccgaacg 
      gaagaaaag aagaaactc gaacgcaaa aaaattgaa 
      gaagcacaa gtcgccagc aaaatccaa atgacggtg 
      gccatcgac gacttcagc aaagaaata gaaatagtc 
      ccttcgaac ctccacccc cagccccct cgacgcacc 
      aaaagcctc gtccactta tttatccca gctgaagat 
      tacgattac ggtaacaag gagttattt ttcgaaaac 
      gaaacaccg tacactagt gaagaaggc tccgattcg 
      ctcctaagt gcctccaag taccaacta ccacgggtg 
      gaaaagggg cgcaagccc gtccaccgg tcggagtcc 
      ttcagacaa gccaagcag ccccaaaac cccgtctcg 
      aaacagaac agcttcgac gggggttat ttcagcgtg 
      ggggttgtc gccgaagac tcgaaaaaa atcaagtcg 
      aagagtttg gaccgaatc gacgacggt ttagagact 
      ttggttgat attgtggtc acagcagaa cacgaagtc 
      cccaagcct gagtcgctc ctcaaccac cagaagaag 
      ttcagtgat gagaaaatg aagatgttt ttgcccaag 
      cgcgacaat gacgtgctt tattacttc ccgaggata 
      caagagcgg aaaaataac acgttcctg gcgcggggc 
      cacaacgcc gggttgtac tcggggcaa gtgacaaga 
      caaaactcg gtgaagaaa ccggagttt gtctcgggg 
      aataaccgg gggaaagtg acggattta ccgtcggga 
      ctttactga tttaatgta aataattat ttatctgtg 
      caatattag agtacacga ggttagggt ttgaggggg 
      cacttgggc cgttcttgg cgagaacgg gccccaagt 
      ggcggaatg tacaaaaag gggtagatt atgatctcg 
      agtgtctat attttaata aaatggttc tcaaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY