Status
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Gene information
General information
TC003067 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2810112..2813496
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003067
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10674

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003067 RA
      MACEYKRQL SQIFDDSLV SEQQIENAI SIVLDNNPL 
      TPEENLEEM INLLVESGD STLSTSEGA AGVTQPNSR 
      QFSQCFDRL TMLFPDICP NYLTQFCQN YDPFDFDQV 
      VEDLSKTDY VKVDKDPLD VWEHLKQML PNADPIYLR 
      NQARVLVNR PTGELEQFI ENAIQENNY PTMQEYLKN 
      KELAAGVAL YEKNFSVDA YLKEIPNPV EIFTNPNRK 
      SPLVDENDL EFALNFLYN QFLFLRKRE IVKIFDHFG 
      KNLVKTCHK LKTLTKAFR HPRPAVELN ECKNIELLK 
      EVAFIKYEK QIRQMLKQQ DDKYRLALE AAREQGLLQ 
      TCACCFDDN LIPEECYFC IKGCIFCKD CVRSGAENV 
      IGKEETRFP CLADCDSEF DYSTLHMVL DRKVFQGVW 
      HRKQIEEIR NANIDGLET CPFCDFCMI PDEGDKIFK 
      CANIECMAE TCRLCRHRS HVPKRCNEI EYDEDVKMR 
      TFIENKMTE ALLRKCWKC SKQFYKESG CNKMTCVCG 
      AMMCYVCGE AVTDYRHFG AGRCPLHTE NLQQFHLTR 
      VLEGAQSAK NELGITGNP NLLKFDPTR GIENQVA
    CDS sequence
    • >TC003067 RA
      atggcttgt gaatacaag cggcagttg agtcaaatc 
      tttgatgat tctctcgta tcagaacaa caaatagaa 
      aacgcgatt agtattgtt ctggacaac aatccttta 
      acacctgag gaaaattta gaggaaatg attaatctt 
      ctggtagag tcaggagac tcaacactt tctacttct 
      gagggggct gctggggtc actcagccg aacagtagg 
      cagttcagt cagtgcttt gataggctg acaatgctg 
      ttccctgat atttgcccc aattattta acccaattt 
      tgccaaaac tacgaccct ttcgacttt gaccaagtt 
      gtggaggac ttatctaaa actgattac gttaaagtt 
      gataaagac ccgttggac gtttgggaa cacctcaag 
      caaatgctg ccaaacgct gatccaatt tatttaaga 
      aaccaagcg agagtttta gtcaataga cctacagga 
      gagttggaa cagttcata gaaaatgcg attcaagaa 
      aacaattat cccacaatg caggaatat ttaaagaat 
      aaggagttg gcagctggg gtggctttg tacgaaaaa 
      aacttttca gttgatgct tatctcaag gaaattcca 
      aatccggtt gaaattttc acgaacccc aatcgcaaa 
      tctccttta gtggacgaa aatgatcta gagtttgcc 
      ttaaatttt ctctataac cagttttta ttcctacgt 
      aagcgcgaa attgtcaag attttcgac cactttggc 
      aaaaatttg gtgaaaact tgtcacaaa ctgaaaact 
      ttgaccaaa gcatttaga caccctagg ccggctgtt 
      gagctaaac gagtgcaaa aatatcgag ttactgaaa 
      gaggttgcg ttcattaaa tacgagaaa cagattaga 
      caaatgttg aaacaacaa gatgataag taccgactt 
      gccttggag gcagcaaga gaacaaggg cttttgcaa 
      acttgtgct tgctgcttc gacgataat ttaattcca 
      gaagagtgt tatttttgc atcaaggga tgtattttt 
      tgcaaggat tgtgtcaga tctggggct gagaacgta 
      atagggaag gaggaaacg aggtttccc tgccttgca 
      gattgtgat tcagagttt gattattcg actctacac 
      atggttttg gaccgtaaa gtgttccaa ggcgtttgg 
      caccgaaaa caaatcgaa gaaatacgc aatgccaac 
      attgacggt ttggaaact tgtccattc tgcgatttc 
      tgtatgatt cctgacgaa ggagacaag atttttaag 
      tgtgccaac attgaatgc atggctgaa acatgccgc 
      ctttgtcgc cataggtcc cacgtgcct aaaagatgc 
      aacgaaatc gaatacgac gaagatgtt aaaatgcga 
      actttcatc gaaaataaa atgactgag gcacttctc 
      aggaagtgt tggaagtgc tctaaacaa ttttacaaa 
      gaatcaggc tgtaataaa atgacttgc gtctgtggt 
      gccatgatg tgctatgtt tgtggggaa gctgtgacg 
      gattatagg cactttgga gctggacgg tgtcctttg 
      catactgaa aatttgcaa cagtttcac ctaactagg 
      gtactagaa ggtgcccaa tcggccaaa aatgagttg 
      ggaattaca ggaaatcca aatctgctt aaatttgat 
      ccaacaagg ggcatcgag aatcaagtg gcttag
    mRNA sequence
    • >TC003067 RA
      aatgtgatg atggtggaa ggttaagtt tagtttaat 
      ttgttactt tttttgcta atttttttt acaatcatg 
      gcttgtgaa tacaagcgg cagttgagt caaatcttt 
      gatgattct ctcgtatca gaacaacaa atagaaaac 
      gcgattagt attgttctg gacaacaat cctttaaca 
      cctgaggaa aatttagag gaaatgatt aatcttctg 
      gtagagtca ggagactca acactttct acttctgag 
      ggggctgct ggggtcact cagccgaac agtaggcag 
      ttcagtcag tgctttgat aggctgaca atgctgttc 
      cctgatatt tgccccaat tatttaacc caattttgc 
      caaaactac gaccctttc gactttgac caagttgtg 
      gaggactta tctaaaact gattacgtt aaagttgat 
      aaagacccg ttggacgtt tgggaacac ctcaagcaa 
      atgctgcca aacgctgat ccaatttat ttaagaaac 
      caagcgaga gttttagtc aatagacct acaggagag 
      ttggaacag ttcatagaa aatgcgatt caagaaaac 
      aattatccc acaatgcag gaatattta aagaataag 
      gagttggca gctggggtg gctttgtac gaaaaaaac 
      ttttcagtt gatgcttat ctcaaggaa attccaaat 
      ccggttgaa attttcacg aaccccaat cgcaaatct 
      cctttagtg gacgaaaat gatctagag tttgcctta 
      aattttctc tataaccag tttttattc ctacgtaag 
      cgcgaaatt gtcaagatt ttcgaccac tttggcaaa 
      aatttggtg aaaacttgt cacaaactg aaaactttg 
      accaaagca tttagacac cctaggccg gctgttgag 
      ctaaacgag tgcaaaaat atcgagtta ctgaaagag 
      gttgcgttc attaaatac gagaaacag attagacaa 
      atgttgaaa caacaagat gataagtac cgacttgcc 
      ttggaggca gcaagagaa caagggctt ttgcaaact 
      tgtgcttgc tgcttcgac gataattta attccagaa 
      gagtgttat ttttgcatc aagggatgt attttttgc 
      aaggattgt gtcagatct ggggctgag aacgtaata 
      gggaaggag gaaacgagg tttccctgc cttgcagat 
      tgtgattca gagtttgat tattcgact ctacacatg 
      gttttggac cgtaaagtg ttccaaggc gtttggcac 
      cgaaaacaa atcgaagaa atacgcaat gccaacatt 
      gacggtttg gaaacttgt ccattctgc gatttctgt 
      atgattcct gacgaagga gacaagatt tttaagtgt 
      gccaacatt gaatgcatg gctgaaaca tgccgcctt 
      tgtcgccat aggtcccac gtgcctaaa agatgcaac 
      gaaatcgaa tacgacgaa gatgttaaa atgcgaact 
      ttcatcgaa aataaaatg actgaggca cttctcagg 
      aagtgttgg aagtgctct aaacaattt tacaaagaa 
      tcaggctgt aataaaatg acttgcgtc tgtggtgcc 
      atgatgtgc tatgtttgt ggggaagct gtgacggat 
      tataggcac tttggagct ggacggtgt cctttgcat 
      actgaaaat ttgcaacag tttcaccta actagggta 
      ctagaaggt gcccaatcg gccaaaaat gagttggga 
      attacagga aatccaaat ctgcttaaa tttgatcca 
      acaaggggc atcgagaat caagtggct tagatgcat 
      tgttttaaa cttttggtt tttgtctat catgctgtg 
      gccttattc ttctaataa tagctgtga tcagaatgt 
      tttcctgct caaaataat gtgtttgta gttaggttt 
      ttctcaggc aatagttgt tctaactgt ttatagaat 
      tacaagttt catacggtt tatagattt tatagaata 
      ttgatcatt gttttctta cgatttgta ttagctgtt 
      aaattataa agattgtat gattattgc tcatgtatt 
      tttaataaa ttgtttcta tgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY