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Gene information
General information
TC007185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7881340..7882703
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007185
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10673

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007185 RA
      MLTLMVFSL IGLAHSSAI PPNNLRLDY QVFSDQEDA 
      KLSVPEIIT KYGYPCEEY HVVTPDNYI LTLHRIPHG 
      RTPKNGPKE VAYLQHGIL SSSADWIIS GPEKGLAYV 
      LADEGYDVW MGNARGNKL SRNHTYLNP DTSDEFWDF 
      SWHEIGYYD LPTMIDFVL EQTGKADLF HIGHSQGTT 
      TFYVMTSMR PDYNAKIKA HFSLAPIAY MNHMTSPLM 
      HIIAFWQKP LTVLLNLIG VREFLPSTE FMAMGGNIL 
      CGDDSLTQI LCKNALFAI CGFSPKEMN GTLLPIMSG 
      HTPAGSSTK QFMHYAQEI NSGYFRRFS YGVFQNLQK 
      YGSIWPPSY DLRKITAPV YLLYSKNDW LAGKIDVDR 
      LYKGLANVK GRFMVAEES FNHLDFVFG IRSRELVYN 
      KVISLMAKL
    CDS sequence
    • >TC007185 RA
      atgttgact ttgatggtt ttctctcta attggattg 
      gcccactct tcggccatc ccacctaac aatctccga 
      ctcgattac caggttttc agtgaccag gaagatgcc 
      aaactcagt gtgcccgaa attataacc aaatatggc 
      tatccttgc gaggagtac catgtggtt actcctgat 
      aattacatc ctgacgctt catcgaatc ccccacgga 
      cgcaccccc aaaaacggc cccaaagaa gtagcgtat 
      ctccaacac gggattctc tcctcttca gccgattgg 
      attatttca ggccctgaa aagggccta gcctatgtc 
      ctagccgat gagggttac gacgtttgg atgggcaat 
      gctcgaggg aacaaactt tcaagaaat cacacttat 
      ctgaacccg gacacgtcc gacgaattc tgggatttt 
      agttggcac gaaatcggc tattacgac ctacctaca 
      atgatcgac ttcgtcctg gaacaaacg ggaaaagcc 
      gaccttttc cacattgga cattcgcaa gggactacc 
      actttctac gtcatgacc tcaatgaga cccgactac 
      aacgccaaa atcaaggca catttcagc ctcgcaccc 
      attgcctac atgaaccat atgaccagc cccctaatg 
      cacattatc gccttctgg cagaagccc ttaactgtc 
      cttttgaat ctaattggg gttcgggag tttctacca 
      agtaccgag tttatggca atggggggc aacatcttg 
      tgcggagac gactcccta acccagatt ctttgcaaa 
      aacgcactt tttgcaatt tgtggcttc agtcccaaa 
      gaaatgaac ggtaccctc ctgcccatc atgagtggg 
      cacacacct gccgggtcc tcaaccaag caatttatg 
      cattacgcc caagaaatc aattcgggg tatttccga 
      cgtttcagt tatggtgtg ttccaaaat ttgcaaaag 
      tacggctct atctggccc ccaagctac gatcttagg 
      aaaatcaca gctccggtg tacttgctt tatagtaaa 
      aatgactgg ttggcgggt aaaatcgat gttgataga 
      ttatacaaa gggttggcc aacgtcaaa gggcggttt 
      atggtggcg gaagaatcg ttcaaccac ctggacttt 
      gtgttcggg ataaggtca cgagagctg gtttataat 
      aaagtcata agtttgatg gccaaattg tag
    mRNA sequence
    • >TC007185 RA
      ctcaaaatg ttgactttg atggttttc tctctaatt 
      ggattggcc cactcttcg gccatccca cctaacaat 
      ctccgactc gattaccag gttttcagt gaccaggaa 
      gatgccaaa ctcagtgtg cccgaaatt ataaccaaa 
      tatggctat ccttgcgag gagtaccat gtggttact 
      cctgataat tacatcctg acgcttcat cgaatcccc 
      cacggacgc acccccaaa aacggcccc aaagaagta 
      gcgtatctc caacacggg attctctcc tcttcagcc 
      gattggatt atttcaggc cctgaaaag ggcctagcc 
      tatgtccta gccgatgag ggttacgac gtttggatg 
      ggcaatgct cgagggaac aaactttca agaaatcac 
      acttatctg aacccggac acgtccgac gaattctgg 
      gattttagt tggcacgaa atcggctat tacgaccta 
      cctacaatg atcgacttc gtcctggaa caaacggga 
      aaagccgac cttttccac attggacat tcgcaaggg 
      actaccact ttctacgtc atgacctca atgagaccc 
      gactacaac gccaaaatc aaggcacat ttcagcctc 
      gcacccatt gcctacatg aaccatatg accagcccc 
      ctaatgcac attatcgcc ttctggcag aagccctta 
      actgtcctt ttgaatcta attggggtt cgggagttt 
      ctaccaagt accgagttt atggcaatg gggggcaac 
      atcttgtgc ggagacgac tccctaacc cagattctt 
      tgcaaaaac gcacttttt gcaatttgt ggcttcagt 
      cccaaagaa atgaacggt accctcctg cccatcatg 
      agtgggcac acacctgcc gggtcctca accaagcaa 
      tttatgcat tacgcccaa gaaatcaat tcggggtat 
      ttccgacgt ttcagttat ggtgtgttc caaaatttg 
      caaaagtac ggctctatc tggccccca agctacgat 
      cttaggaaa atcacagct ccggtgtac ttgctttat 
      agtaaaaat gactggttg gcgggtaaa atcgatgtt 
      gatagatta tacaaaggg ttggccaac gtcaaaggg 
      cggtttatg gtggcggaa gaatcgttc aaccacctg 
      gactttgtg ttcgggata aggtcacga gagctggtt 
      tataataaa gtcataagt ttgatggcc aaattgtag 
      aagcgttta cattaaatg cttagcaac aataaaagt 
      ggtgcgtca acagtctct ttatcacag tattaataa 
      attgttaat tataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY