Status
Please wait ...
Gene information
General information
TC014132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6415501..6419366
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014132
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014132 RA
      MSNDKFFTH DENRGTWAS KSEFLLSCM GYAIGIGNV 
      WRFPYLCYR NGGGAFLVP YLLMLFLCG IPLFFLETS 
      LGQFASTGC ITLFKIAPL FKGVGFAIV IVNAICTSY 
      YNVIIAYPL FFLANCFQS KLPWTNCDN SWNTEHCVK 
      LGGRSAVKS HANETFLSH QNETFENRM KTPADEFFH 
      YHILQISDS IEEQGSIVW PLLLCTVIA WIITYLCII 
      KGVKTVGKV VYFTATFPF FVLFILLIR GLTLPGAWD 
      GVFFYIYPQ WHQLTNLKV WADAACQIF FSLGPGWGG 
      IVNMASYNN FRNNNQLDS ILVPILNCG TSIFAGFVV 
      FSVIGFMSH ETGLPISTV ATGGPGLAF VTYPEAITM 
      LPWPHIWAI LFFLMLFFL GLDSCFVQI EAIISSVID 
      EFPALRKHK MMVTLVSCL VMMFLSFLF VTNGGMYLL 
      QLFDWYAAS ISVILICLL EVFIVGWTY GIQQFVKDL 
      EFMIQRKIH WWWIISWKI ITPLILSFI FVTTIAFNT 
      RVTYRGIAF PDWSITLGW CSCGVSIIC IPLYMGYRL 
      LYLEEGDLI ARIQNALKP TIDWGPANK EHRREWFKA 
      VHYDKIDAV TRRTDPYHE QFIQLATMN T
    CDS sequence
    • >TC014132 RA
      atgtccaac gacaaattt tttacacac gatgaaaat 
      cgtggaacc tgggctagc aaatccgaa tttttacta 
      tcttgcatg ggctacgcc ataggtata ggaaacgtg 
      tggcgattt ccatacttg tgttacagg aatggagga 
      ggtgctttt ctcgttcct tatttgtta atgctgttt 
      ctttgcggt attccgctt ttttttctg gaaacctct 
      ctaggacaa tttgcaagc actggatgt atcacttta 
      tttaaaatt gctccatta tttaaaggg gttggcttt 
      gccattgta attgtgaat gctatttgc acctcttat 
      tataacgtt attattgca taccctctg ttctttctg 
      gcaaattgt tttcaatca aaattgcct tggaccaac 
      tgtgacaat tcgtggaac actgaacac tgcgttaag 
      ttaggtggt cgtagcgct gtgaaaagt cacgcgaac 
      gaaactttc ctttcgcac caaaatgaa acatttgaa 
      aataggatg aagactcca gccgacgag tttttccac 
      taccacatt ttgcaaata tcggatagc attgaggaa 
      caaggaagt atcgtttgg ccacttctt ttgtgcact 
      gtaatagct tggatcata acttacctt tgcataata 
      aagggtgtg aaaactgtt ggcaaagtt gtatatttc 
      acagcaacg tttcctttt ttcgtctta ttcattctt 
      ctaataaga gggttaaca ttacctggc gcttgggac 
      ggagtattt ttttacatc tatccacag tggcatcaa 
      cttacaaat ttaaaggtg tgggctgat gctgcttgt 
      caaatcttt ttctctttg ggtccaggc tggggcgga 
      attgttaac atggcaagt tacaacaac tttaggaac 
      aataatcag ctagattca attcttgtg ccaattttg 
      aactgtgga actagtatt tttgctggg ttcgttgtg 
      ttttccgtt atcggtttc atgtctcat gagaccggt 
      ttgccgata tcaacagtc gcaacaggc ggccccgga 
      cttgctttc gtcacatat ccggaagca atcacgatg 
      ttgccttgg cctcacatt tgggcgatt ttgtttttt 
      cttatgctt ttcttccta ggtctggac agctgcttc 
      gttcaaata gaagcgatc atttctagc gtcattgac 
      gagtttcct gcattaagg aaacacaag atgatggta 
      actttggtc tcctgtttg gttatgatg tttctgtcg 
      tttctgttt gtcactaat ggaggaatg tacctttta 
      cagttattt gactggtat gctgcatca atttcggtg 
      attttaatt tgtttatta gaagtgttt attgttgga 
      tggacatat ggtatacaa cagtttgtt aaagattta 
      gaatttatg atacaaagg aaaattcac tggtggtgg 
      attatttca tggaaaatc attactcct ttaattctt 
      tcgtttatt tttgtaacc accatagca tttaacaca 
      agagttaca tacagaggt attgctttc ccggactgg 
      tctattaca ttaggttgg tgttcctgc ggtgtctca 
      attatatgc attcctctt tatatgggc tacagactg 
      ttatatcta gaagaaggt gatttaatt gctcgaatt 
      caaaatgct ttaaaacca actatagac tggggtccc 
      gcaaacaag gaacataga agggaatgg tttaaagct 
      gttcattat gacaaaatt gacgctgta acaagacgt 
      accgatcca tatcacgaa cagtttatt caacttgcg 
      acaatgaat acttaa
    mRNA sequence
    • >TC014132 RA
      gcaaagtaa actacttaa atgtccaac gacaaattt 
      tttacacac gatgaaaat cgtggaacc tgggctagc 
      aaatccgaa tttttacta tcttgcatg ggctacgcc 
      ataggtata ggaaacgtg tggcgattt ccatacttg 
      tgttacagg aatggagga ggtgctttt ctcgttcct 
      tatttgtta atgctgttt ctttgcggt attccgctt 
      ttttttctg gaaacctct ctaggacaa tttgcaagc 
      actggatgt atcacttta tttaaaatt gctccatta 
      tttaaaggg gttggcttt gccattgta attgtgaat 
      gctatttgc acctcttat tataacgtt attattgca 
      taccctctg ttctttctg gcaaattgt tttcaatca 
      aaattgcct tggaccaac tgtgacaat tcgtggaac 
      actgaacac tgcgttaag ttaggtggt cgtagcgct 
      gtgaaaagt cacgcgaac gaaactttc ctttcgcac 
      caaaatgaa acatttgaa aataggatg aagactcca 
      gccgacgag tttttccac taccacatt ttgcaaata 
      tcggatagc attgaggaa caaggaagt atcgtttgg 
      ccacttctt ttgtgcact gtaatagct tggatcata 
      acttacctt tgcataata aagggtgtg aaaactgtt 
      ggcaaagtt gtatatttc acagcaacg tttcctttt 
      ttcgtctta ttcattctt ctaataaga gggttaaca 
      ttacctggc gcttgggac ggagtattt ttttacatc 
      tatccacag tggcatcaa cttacaaat ttaaaggtg 
      tgggctgat gctgcttgt caaatcttt ttctctttg 
      ggtccaggc tggggcgga attgttaac atggcaagt 
      tacaacaac tttaggaac aataatcag ctagattca 
      attcttgtg ccaattttg aactgtgga actagtatt 
      tttgctggg ttcgttgtg ttttccgtt atcggtttc 
      atgtctcat gagaccggt ttgccgata tcaacagtc 
      gcaacaggc ggccccgga cttgctttc gtcacatat 
      ccggaagca atcacgatg ttgccttgg cctcacatt 
      tgggcgatt ttgtttttt cttatgctt ttcttccta 
      ggtctggac agctgcttc gttcaaata gaagcgatc 
      atttctagc gtcattgac gagtttcct gcattaagg 
      aaacacaag atgatggta actttggtc tcctgtttg 
      gttatgatg tttctgtcg tttctgttt gtcactaat 
      ggaggaatg tacctttta cagttattt gactggtat 
      gctgcatca atttcggtg attttaatt tgtttatta 
      gaagtgttt attgttgga tggacatat ggtatacaa 
      cagtttgtt aaagattta gaatttatg atacaaagg 
      aaaattcac tggtggtgg attatttca tggaaaatc 
      attactcct ttaattctt tcgtttatt tttgtaacc 
      accatagca tttaacaca agagttaca tacagaggt 
      attgctttc ccggactgg tctattaca ttaggttgg 
      tgttcctgc ggtgtctca attatatgc attcctctt 
      tatatgggc tacagactg ttatatcta gaagaaggt 
      gatttaatt gctcgaatt caaaatgct ttaaaacca 
      actatagac tggggtccc gcaaacaag gaacataga 
      agggaatgg tttaaagct gttcattat gacaaaatt 
      gacgctgta acaagacgt accgatcca tatcacgaa 
      cagtttatt caacttgcg acaatgaat acttaaatt 
      taattttgt tttacatca aaataacgt tttcacact 
      gttgtcttt acaattagc attaagatg aagacgacc 
      caggaagcg ttataattt aatattacc cattttaat 
      gcaataatt gcggaaatt attggacgt gtaaacata 
      cctcagttt tgcaactag ctgccagtt aatgaacgg 
      tttacgaag gatatctgc gtgcaagta aagaaaatc 
      aagaataaa attttgcat gattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY