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Gene information
General information
TC010133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14202869..14207366
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010133
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10671

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010133 RA
      MSYKISMKN GINCTEAQW IKSNNLVDT 
      SVKLAGEEA KDRLYEPKH KKKVVRVLT 
      VIAYVFFVS LAAIMLSLY YVFLWNGGQ 
      KTLPKYPIQ REAQMKCDI VAEMQQQIT 
      NVATNEVFS TPSSTEENT TSPGFGLSF 
      DNDEFEKTS TFMKKIMYK YPYDEND
    CDS sequence
    • >TC010133 RA
      atgtcttac aaaatctcc atgaaaaat ggcatcaac 
      tgcacggaa gcacagtgg attaagagc aacaatttg 
      gtggacact agtgttaaa cttgcgggc gaggaagcc 
      aaagaccga ctttacgag cccaagcac aaaaagaag 
      gttgtgaga gtgttaaca gtcatagct tatgttttt 
      ttcgtttcg ttggcggca attatgctc tcgctgtat 
      tacgttttc ttgtggaac ggaggacaa aagactttg 
      cccaaatat ccgatacag cgcgaagcg caaatgaaa 
      tgcgacatt gttgcagag atgcaacaa cagatcacc 
      aacgttgca acaaacgag gttttttcc actccctcg 
      tccacggag gaaaacacc acgtcgccg ggcttcgga 
      ctcagcttc gacaatgat gaattcgag aaaacaagc 
      acatttatg aaaaaaatt atgtacaaa tacccctac 
      gatgaaaac gactag
    mRNA sequence
    • >TC010133 RA
      agaacttca ggaactcgt aaattttca cattcgatg 
      gtttggcgc ttcctggcg ttaaaattt gaattttgt 
      aatgcattt gcgaggtaa gaatttcaa attttttca 
      aactttgga cgaaagtgg cagttttag cggaatgaa 
      ccggtagac ggaaatgtc atcggtgga ggacataaa 
      tgtcgacga ctcgccgga atcgcctgt ggattcaaa 
      ggacaatgc acttttcga gccaaaagc tcagccgaa 
      gattattcg ggtactcac cgtgttagc ctacatact 
      cagcgtgtc catggcagc catcctctt atcaatcta 
      ttatatctt tatgtggaa cgaacacaa aacttatgc 
      cctgcataa tgaatttgt gccccccgg cgccgcatc 
      ttcgcttat ggaggaagc caactttac cactcctac 
      cttcaacac agaccaact tccacaaat agatacaaa 
      ttcagattc agacaaaag acttcactc tgataaaaa 
      tttaaaata tcaattggt agtgggtac ttttgttgt 
      actacgtaa gaaatacga gtatttcga taaaataaa 
      cgcaataat ttttgaaaa aagaaaatg gcaattgtt 
      tggtctatg aaaaatgag agaacacct attttaagc 
      gtgcttgca taaaagtaa aaatatctt cctcgacgg 
      ttgttacat tactactga actgtgttc gagcgaatt 
      ccaatcaca caagatatt tttcttaaa atatcggtc 
      tttggattt taaataaaa aaaaatgga aaacccctt 
      ttgaaaatt gtggaggtt gcgcatgga atccttgtc 
      ctggtttgc tccaaacag ctgtaattt attgattga 
      cccggtgtg aaagatgcg aaaaccaaa acaatagtg 
      tcctgattt gaaaagcaa gtagcaagc gactgtttc 
      gaacgctgt aaaatgatc gattgttgc aaagaatcc 
      ccccaagca gacaaacgt aaacgaata gaaataact 
      cataataac cagaccgca ttagtgtac attattatc 
      aaaaagcag taattcagc ccaatccat cagcgtggc 
      gtgtaaatt cctcaaagc tctcctcat tttcaaatc 
      aaaaatgtc ttacaaaat ctccatgaa aaatggcat 
      caactgcac ggaagcaca gtggattaa gagcaacaa 
      tttggtgga cactagtgt taaacttgc gggcgagga 
      agccaaaga ccgacttta cgagcccaa gcacaaaaa 
      gaaggttgt gagagtgtt aacagtcat agcttatgt 
      ttttttcgt ttcgttggc ggcaattat gctctcgct 
      gtattacgt tttcttgtg gaacggagg acaaaagac 
      tttgcccaa atatccgat acagcgcga agcgcaaat 
      gaaatgcga cattgttgc agagatgca acaacagat 
      caccaacgt tgcaacaaa cgaggtttt ttccactcc 
      ctcgtccac ggaggaaaa caccacgtc gccgggctt 
      cggactcag cttcgacaa tgatgaatt cgagaaaac 
      aagcacatt tatgaaaaa aattatgta caaataccc 
      ctacgatga aaacgacta gggcaaaca aatacttcc 
      tgtttgctc ccatgacgt gtttacatt atacgcatt 
      acttcgtaa tagggttta ttctgtatt tttagctaa 
      cattttgat atcatcatt aatttattt ttataaaag 
      atattgcgg cgttttacc aaattatgg taggttcta 
      gaatagaaa aaaaggttt gcgacttga ttatttata 
      aggcttgag gataaaatc ttttattga aggttgatt 
      ggttacagt tttcttttt ttttctgga aaagttttc 
      acaggtctc gaattttgt gtaaatttt tccactatt 
      agtcacagg attaaaaag aaaaacagt ttccagagc 
      gtttacagt attttagaa tgaaggtat tggtaacat 
      aaatcatct tcatccaaa tgtttatca tcgctcaac 
      tttcattca ctcatgtgt gagcttata aacagatgg 
      acacacgtg caatacttc aaaattaat ccaacgcgc 
      attatgtaa gattgcgaa aattaaaaa aaaaagtta 
      tgaaagaga ttactatta tcttatgac ggaggtttg 
      caattgaag ggaaccgga agtctgttc aacttaaag 
      aggttcgaa aattgattt ctattagaa aaaacaaaa 
      cacttcgac agattattg aaatttcgg gatctaaaa 
      gttcaactt atcaaggtt ccactgtag ttatttatt 
      attatgtgt tctacaggt cgccatttt tattaaagc 
      actcttcca aattttggc gattttttg acattttat 
      cgtgtcttc agtcttatt tggcaaaag tttgaccat 
      tttgtcgta ggcatacgg tttttaaac aagcttttg 
      gcagaatta agtgtggga attcaaatt tggcgccag 
      tttcattat agcactcat ttaaatttt gacgatttt 
      ctcccattt ttttataga aacacggtt cctgaatgc 
      agtatttgg cagaaagtt gcatatttt ctttaattt 
      tggcaattt tctgttact tattaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY